Abstract：
:This study examined how the difference in pore size of porous scaffolds affected the in vitro chondrogenic differentiation of seeded adipose stem cells (ASCs) and the in vivo cartilage repair of ASC/scaffold construct. ASCs were isolated from 18 rabbits and seeded in a porous poly (ε-caprolactone) (PCL) scaffold with different pore sizes (100, 200, 400 μm). The ASCs underwent in vitro chondrogenic induction under TGF-β2 and BMP-7 for 21 days before analysis. The ASC/scaffold construct was also implanted on the osteochondral defect created on the distal femur of the same rabbits, and the quality of cartilage regeneration was analyzed after 8 weeks. At day 21, the ASCs proliferated and spread on the surface of the scaffolds with a pore size 100 and 200 μm, whereas there were many lumps of conglomerated ASCs on those with a pore size of 400 μm. The DNA content was significantly lower in the scaffold with a pore size of 400 μm than in that with a pore size of 100 or 200 μm. Proteoglycan production was significantly greater in the scaffold with a pore size of 400 and 200 μm than in that with a pore size of 100 μm. The chondrogenic marker gene expression including SOX9 and COL2A1 was greatest in the scaffold with a pore size of 400 μm followed by 200 μm. Immunofluorescent imaging showed that, while SOX9 was localized to nucleus, type II collagen was observed on the cytoplasm and secreted matrix around the cells most abundantly in the scaffold with a pore size of 400 μm followed by 200 μm. The gross and histological findings from the osteochondral defects showed that the cartilage repair was better in the scaffold with a pore size of 400 and 200 μm than in that with a pore size of 100 μm.
journal_name
Cell Transplantjournal_title
Cell transplantationauthors
Im GI,Ko JY,Lee JHdoi
10.3727/096368912X638865subject
Has Abstractpub_date
2012-01-01 00:00:00pages
2397-405issue
11eissn
0963-6897issn
1555-3892pii
ct0629imjournal_volume
21pub_type
杂志文章abstract：:Facioscapulohumeral muscular dystrophy (FSHD) is the third most frequent inherited myopathy. We previously demonstrated that mesoangioblasts can be efficiently isolated from FSHD muscles, although their differentiation ability into skeletal muscle was variably impaired. This correlates with overall disease severity a...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368910X546571
更新日期：2011-01-01 00:00:00
abstract：:The main purpose of this work has been to establish a new culturing technique to improve the chondrogenic commitment of isolated adult human chondrocytes, with the aim of being used during cell-based therapies or tissue engineering strategies. By using a rather novel technique to generate scaffold-free three-dimension...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368908786576499
更新日期：2008-01-01 00:00:00
abstract：:The cytology of peritoneal washing fluids for gastric cancer is the most basic method for judging peritoneal micrometastasis. However, the clinical value of this method is not clear at present. A retrospective analysis was performed on 277 patients with pathologically proven and surgically treated gastric cancer. The ...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1177/0963689719864318
更新日期：2019-11-01 00:00:00
abstract：:Ten mongrel dogs were used in this study. Diabetes was chemically induced in 7 dogs, and 3 dogs served as normal controls. For each diabetic dog, 5 million human bone marrow-derived mesenchymal stem cells/kg were differentiated to form insulin-producing cells using a trichostatin-based protocol. Cells were then loaded...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1177/0963689718759913
更新日期：2018-06-01 00:00:00
abstract：:Pancreatic beta cell lines may offer an abundant source of cells for beta-cell replacement in type I diabetes. Using regulatory elements of the bacterial tetracycline (tet) operon for conditional expression of SV40 T antigen oncoprotein in transgenic mouse beta cells, we have shown that reversible immortalization is a...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：:Embryonic stem (ES) cells have the ability to generate teratomas when transplanted into immunodeficient mice, but conditions affecting the generation remain to be elucidated. Nonhuman primate cynomolgus ES cells were transplanted into immunodeficient mice under different conditions; the number of transplanted cells, p...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368908786991560
更新日期：2008-09-01 00:00:00
abstract：:Sciatic nerve injuries, not uncommon in trauma with a limited degree of functional recovery, are considered a persistent clinical, social, and economic problem worldwide. Accumulating evidence suggests that stem cells can promote the tissue regeneration through various mechanisms. The aim of the present study was to i...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1177/0963689720919438
更新日期：2020-01-01 00:00:00
abstract：:During the isolation of human islets of Langerhans the digest has repeated direct contact with the ambient atmosphere. In order to fulfill GMP requirements in clinical applications, the entire cell preparation must be performed in clean room facilities. We hypothesized that the use of a closed system, which avoids the...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/000000007783465091
更新日期：2007-01-01 00:00:00
abstract：:Multiple sclerosis is a disease characterized by inflammation and demyelination located in the central nervous system. Experimental autoimmune encephalomyelitis (EAE) is the most common animal model for multiple sclerosis (MS). Although the roles of T cells in MS/EAE have been well investigated, little is known about ...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1177/0963689719852354
更新日期：2019-09-01 00:00:00
abstract：:The midbrain inferior colliculus (IC), a major integrating center for auditory processing, provides a model for structural, functional, and behavioral recovery. The present study examined the role of IC in spatial sound detection, and the effects of neural transplantation in sparing of behavioral performance. Hooded r...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1016/0963-6897(95)02036-5
更新日期：1996-03-01 00:00:00
abstract：:Clinically, many candidates for islet transplantation are already immunized, which increases their risk of graft rejection. Encapsulation of pancreatic islets using the TheraCyte™ device has been shown to protect against allograft rejection in nonimmunized recipients. However, the capacity of the TheraCyte™ device to ...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368912X657486
更新日期：2013-01-01 00:00:00
abstract：:This study was designed to investigate functional recovery after the transplantation of mesenchymal stem cells (MSCs) or neurally differentiated MSCs (NMSCs) derived from bone marrow in a rat model of spinal cord injury (SCI). Sprague-Dawley rats were subjected to incomplete SCI using an NYU impactor to create a free ...
journal_title：Cell transplantation
pub_type： 杂志文章,已发布勘误
doi：10.3727/096368916X692078
更新日期：2016-07-01 00:00:00
abstract：:Nonhuman primate models of Parkinson's disease (PD) have been invaluable to our understanding of the human disease and in the advancement of novel therapies for its treatment. In this review, we attempt to give a brief overview of the animal models of PD currently used, with a more comprehensive focus on the advantage...
journal_title：Cell transplantation
pub_type： 杂志文章,评审
doi：10.3727/000000006783982025
更新日期：2006-01-01 00:00:00
abstract：:Porcine neonatal islet-like cell clusters (NICCs) may be an attractive source of insulin-producing tissue for xenotransplantation in type I diabetic patients. We examined the functional and immunohistochemical outcome of the islet grafts in vitro during long-term culture and in vivo after transplantation to athymic nu...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/000000003783985142
更新日期：2003-01-01 00:00:00
abstract：:Intrauterine inflammation is a significant cause of injury to the developing fetal brain. Using a preterm fetal sheep model of in utero infection, we asked whether human amnion epithelial cells (hAECs) were able to reduce inflammation-induced fetal brain injury. Surgery was undertaken on pregnant sheep at ∼105 days ge...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368916X693572
更新日期：2017-04-13 00:00:00
abstract：:Cardiovascular diseases are related to many risk factors, such as diabetes, high blood pressure, smoking, and obesity. Myocardial infarction (MI), a cardiovascular disease, is the most common cause of cardiomyocyte death. In MI, hypoxia induces cardiomyocyte apoptosis; in particular, diabetes combined with MI has a sy...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368915X686995
更新日期：2015-01-01 00:00:00
abstract：:Airway epithelial cell injury is a key triggering event to activate allergic airway inflammation, such as asthma. We previously reported that administration of mesenchymal stem cells (MSCs) significantly alleviated allergic inflammation in a mouse model of asthma, and the mmu-miR-21/ACVR2A axis may be involved. Howeve...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1177/0963689718767159
更新日期：2018-03-01 00:00:00
abstract：:The nonobese diabetic (NOD) mouse model of type 1 diabetes (T1D) was discovered by coincidence in the 1980s and has since been widely used in the investigation of T1D and diabetic complications. The current in vivo study was originally designed to prospectively assess whether hyperglycemia onset is associated with phy...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1177/0963689720939127
更新日期：2020-01-01 00:00:00
abstract：:Hematopoietic stem cells (HSCs) migrate to injury sites and aid in tissue repair. However, clinical success is poor and is partially due to limited HSC recruitment. We hypothesized that HSC pretreatment with H2O2 would enhance their recruitment to injured gut. As HSCs are rare cells, the number of primary cells obtain...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368912X653192
更新日期：2013-01-01 00:00:00
abstract：:Schwann cells naturally support axonal regeneration after injury in the peripheral nervous system, and have also shown a significant, albeit limited, ability to support axonal growth and remyelination after grafting to the central nervous system (CNS). It is possible that Schwann cell-induced axonal growth in the CNS ...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1016/s0963-6897(97)00166-8
更新日期：1998-03-01 00:00:00
abstract：:The present study aimed to elucidate the mechanisms by which hydrogen sulfide (H2S) attenuates left ventricular remodeling after myocardial infarction (MI). MI was created in mice by left coronary artery ligation. One group of mice received injections of the H2S donor sodium hydrosulfide (NaHS) immediately before and ...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368917X695029
更新日期：2017-05-09 00:00:00
abstract：:Magnetic targeting has the potential to enhance the therapeutic effects of stem cells through increasing retention of transplanted cells. To investigate the effects of magnetic targeting intensities on cell transplantation, we performed different magnetic intensities for mesenchymal stem cell (MSC)-targeting therapy i...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368914X685302
更新日期：2015-01-01 00:00:00
abstract：:A human embryonic stem cell (HESC) line, H1, was studied after differentiation to a dopaminergic phenotype in vitro in order to carry out in vivo studies in Parkinsonian monkeys. To identify morphological characteristics of transplanted donor cells, HESCs were transfected with a GFP lentiviral vector. Gene expression ...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368913X664865
更新日期：2014-01-01 00:00:00
abstract：:Superparamagnetic iron oxide nanoparticles (MNPs) together with magnetic resonance imaging (MRI) are the preferred tools for monitoring the fate and biodistribution of administered cells in stem cell therapy studies. Commercial MNPs need transfection agents and long incubation times for sufficient cell labeling and fu...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368916X691303
更新日期：2016-10-01 00:00:00
abstract：:Transplantation therapies aimed at repairing neurodegenerative and neuropathological conditions of the central nervous system (CNS) have utilized and tested a variety of cell candidates, each with its own unique set of advantages and disadvantages. The use and popularity of each cell type is guided by a number of fact...
journal_title：Cell transplantation
pub_type： 杂志文章,评审
doi：10.3727/096368912X655208
更新日期：2013-01-01 00:00:00
abstract：:Glucocorticoids (GCs) are used as immunosuppressive and antiinflammatory agents in organ transplantation and in treating autoimmune diseases and inflammatory disorders. GCs were shown to exert their antiproliferative effects directly through blockade of certain elements of an early membrane-associated signal transduct...
journal_title：Cell transplantation
pub_type： 杂志文章,评审
doi：10.1016/s0963-6897(98)00042-6
更新日期：1998-11-01 00:00:00
abstract：:Neural progenitor cells (NPCs) overexpressing fibroblast growth factor 2 (FGF-2) have the distinct tendency to associate with the vasculature and establish multiple proliferative clusters in the perivascular environment after transplantation into the cerebral cortex. Strikingly, the vascular clusters of progenitor cel...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368916X690421
更新日期：2016-01-01 00:00:00
abstract：:The recent advent of endoscopy has enabled the endoscopic submucosal dissection (ESD) of superficial nonampullary duodenal epithelial tumors. However, the substantially thin wall and presence of bile and pancreatic juice make it technically difficult to perform duodenal ESD without perforation, which leads to lethal c...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1177/0963689720963882
更新日期：2020-01-01 00:00:00
abstract：:Liver fibrosis is characterized by excessive accumulation of extracellular matrix components in the liver parenchyma that distorts the normal architecture and hepatic function. Progressive fibrosis could end in the advanced stage known as cirrhosis, resulting in the need to resort to liver transplantation. Amniotic me...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368916X692645
更新日期：2016-12-13 00:00:00
abstract：:The effect of tensile stress on the orientation and phenotype of arterial smooth muscle cells (SMCs) cultured in three-dimensional (3D) type I collagen gels was morphologically investigated. Ring-shaped hybrid tissues were prepared by thermal gelation of a cold mixed solution of type I collagen and SMCs derived from b...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1177/096368979400300605
更新日期：1994-11-01 00:00:00
