Abstract:
:The recent advent of endoscopy has enabled the endoscopic submucosal dissection (ESD) of superficial nonampullary duodenal epithelial tumors. However, the substantially thin wall and presence of bile and pancreatic juice make it technically difficult to perform duodenal ESD without perforation, which leads to lethal complications. The present study evaluated the efficacy of autologous myoblast sheet transplantation for the prevention of late perforation after duodenal ESD in a porcine model. Two weeks before ESD, skeletal muscle was surgically excised from the femur of pigs, and myoblasts were isolated and seeded in temperature-responsive culture dishes to prepare sheets. Immediately after ESD, the autologous myoblast sheets were attached to the serosal surface at the ESD site with omentopexy. The pigs were divided into two groups: the autologous myoblast sheet group (n = 5), where the myoblast cell sheet was attached to the ESD ulcer part from the duodenal serous side, and the Omentum group (n = 5), where only the omentum was used. The pigs were sacrificed and analyzed macroscopically and histologically on postoperative day 3. The macroscopic examination of the abdominal cavity revealed perforation in the ESD ulcer area and leakage of bile in the Omentum group but no perforation in the Sheet group. A histopathological examination revealed that continuity of the duodenal wall at the ESD site was maintained with dense connective tissue in the Sheet group. In conclusion, autologous myoblast sheets were useful for preventing perforation after duodenal ESD.
journal_name
Cell Transplantjournal_title
Cell transplantationauthors
Matsumoto R,Kanetaka K,Maruya Y,Yamaguchi S,Kobayashi S,Miyamoto D,Ohnita K,Sakai Y,Hashiguchi K,Nakao K,Eguchi Sdoi
10.1177/0963689720963882subject
Has Abstractpub_date
2020-01-01 00:00:00pages
963689720963882eissn
0963-6897issn
1555-3892journal_volume
29pub_type
杂志文章abstract::Bone marrow (BM) cells may interact with coronary endothelium and modulate coronary atherosclerosis. We investigated the time course of coronary luminal loss and changes in conductance after intracoronary injection of enriched hematopoietic BM stem cells in patients with previous myocardial infarction (MI). Among 24 p...
journal_title:Cell transplantation
pub_type: 杂志文章,随机对照试验
doi:10.3727/096368907783338244
更新日期:2007-01-01 00:00:00
abstract::Pancreas procurement for islet isolation and transplantation is limited by concerns for the detrimental effects of postmortem ischemia. Hypothermic machine perfusion (HMP) preservation technology has had a major impact in circumventing ischemic injury in clinical kidney transplantation and is applied here to the prese...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368910X486316
更新日期:2010-01-01 00:00:00
abstract::Neuroblastoma, a malignant tumor of the sympathetic nervous system, is an aggressive extracranial tumor in childhood. Long noncoding RNAs (lncRNAs) have been discovered to play a key role in the eukaryotic regulatory gene network and be involved in a wide variety of biological processes. We observed that the expressio...
journal_title:Cell transplantation
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abstract::The nonobese diabetic (NOD) mouse model of type 1 diabetes (T1D) was discovered by coincidence in the 1980s and has since been widely used in the investigation of T1D and diabetic complications. The current in vivo study was originally designed to prospectively assess whether hyperglycemia onset is associated with phy...
journal_title:Cell transplantation
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更新日期:2020-01-01 00:00:00
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journal_title:Cell transplantation
pub_type: 杂志文章
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abstract::Diabetes affects millions of people worldwide, and β-cell replacement is one of the promising new strategies for treatment. Induced pluripotent stem cells (iPSCs) can differentiate into any cell type, including pancreatic β cells, providing a potential treatment for diabetes. However, the molecular mechanisms underlyi...
journal_title:Cell transplantation
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journal_title:Cell transplantation
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更新日期:2014-01-01 00:00:00
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journal_title:Cell transplantation
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abstract::Nonhuman primate models of Parkinson's disease (PD) have been invaluable to our understanding of the human disease and in the advancement of novel therapies for its treatment. In this review, we attempt to give a brief overview of the animal models of PD currently used, with a more comprehensive focus on the advantage...
journal_title:Cell transplantation
pub_type: 杂志文章,评审
doi:10.3727/000000006783982025
更新日期:2006-01-01 00:00:00
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journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368979400300413
更新日期:1994-07-01 00:00:00
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journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368908786991588
更新日期:2008-01-01 00:00:00
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journal_title:Cell transplantation
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doi:10.1177/0963689718784878
更新日期:2018-08-01 00:00:00
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journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689719883842
更新日期:2019-12-01 00:00:00
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journal_title:Cell transplantation
pub_type: 杂志文章,随机对照试验
doi:10.3727/096368914X678562
更新日期:2014-01-01 00:00:00
abstract::Peripheral blood samples from HIV-seropositive individuals enrolled in a pilot clinical trial investigating the use of allogeneic dendritic cell therapy were evaluated for mixed chimerism. In this study, dendritic cells from HLA-identical, HIV-seronegative siblings were used. Patients received an infusion of dendritic...
journal_title:Cell transplantation
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:2000-05-01 00:00:00
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journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1016/0963-6897(95)00001-e
更新日期:1995-05-01 00:00:00
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journal_title:Cell transplantation
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doi:10.3727/096368913X669770
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doi:10.3727/096368911X605330
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journal_title:Cell transplantation
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更新日期:2015-01-01 00:00:00
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journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000008783906955
更新日期:2008-01-01 00:00:00
abstract::Protocols of donor bone marrow infusion for tolerance induction are receiving increasing attention in clinical trials of organ allotransplantation. We report pulmonary embolism with bone fragments following vertebral body marrow infusion in a recipient of a liver and intestinal transplant. Even though pulmonary emboli...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1016/0963-6897(96)00026-7
更新日期:1996-07-01 00:00:00
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更新日期:2013-01-01 00:00:00
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journal_title:Cell transplantation
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abstract::In this study the time course of homing and the body distribution of systemically delivered bone marrow mesenchymal stem cells (BM-MSCs) after myocardial infarction (MI) were evaluated. BM-MSCs were isolated from Wistar rats, expanded in vitro, and their phenotypical characterization was performed by flow cytometer. R...
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更新日期:2010-01-01 00:00:00
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