Abstract:
:Peripheral blood samples from HIV-seropositive individuals enrolled in a pilot clinical trial investigating the use of allogeneic dendritic cell therapy were evaluated for mixed chimerism. In this study, dendritic cells from HLA-identical, HIV-seronegative siblings were used. Patients received an infusion of dendritic cells pulsed with HIV MN gp160 protein or with peptides from HLA-A2 restricted epitopes of env, gag, and pol proteins every month for 6-9 months. Of the five allogeneic dendritic cell recipients, two showed increases in HIV antigen-specific immune responses. Allele-specific polymorphisms were identified in three sib-pairs that allowed infused donor cells to be detected using sensitive PCR-based molecular methods. Analysis of blood samples from patients showed similar patterns of donor cell persistence after the first infusion, in that cells were detectable for at least 1 week. Also, differences were observed in the kinetics of cell survival between the first and subsequent infusion cycles in all three patients. This suggests variation in HIV-specific immune responses detected among these three patients was not due to differences in persistence of infused donor cells.
journal_name
Cell Transplantjournal_title
Cell transplantationauthors
Shapero MH,Kundu SK,Engleman E,Laus R,van Schooten WC,Merigan TCsubject
Has Abstractpub_date
2000-05-01 00:00:00pages
307-17issue
3eissn
0963-6897issn
1555-3892journal_volume
9pub_type
临床试验,杂志文章,随机对照试验abstract::Human marrow stromal cells (hMSCs) provide functional benefit in rats subjected to stroke. Astrocytes are coupled into a cellular network via gap junction channels, predominantly composed of connexin-43 (Cx43) proteins. Astrocytes are believed to play a vital role in neuroprotection by providing energy substrates to n...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000005783983205
更新日期:2005-01-01 00:00:00
abstract::Pluripotent stem cells, including human embryonic stem cells and induced pluripotent stem cells, have generated much excitement about their prospects for use in cell transplantation therapies. This is largely attributable to their virtually unlimited growth potential, their ability to be precisely genetically altered ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368910X498944
更新日期:2010-01-01 00:00:00
abstract::Autism spectrum disorders (ASDs) are heterogeneous complex neurodevelopmental pathologies defined by behavioral symptoms, but which have well-characterized genetic, immunological, and physiological comorbidities. Despite extensive research efforts, there are presently no agreed upon therapeutic approaches for either t...
journal_title:Cell transplantation
pub_type: 临床试验,杂志文章
doi:10.3727/096368914X684916
更新日期:2014-01-01 00:00:00
abstract::Expression of a fluorescent reporter gene has been studied using two alternate promoters to transcribe the green fluorescent protein (gfp) from Aequorea victoria. The human cytomegalovirus (CMV) enhancer/ promoter or the human muscle-specific creatine kinase promoter (CKM) were inserted along with the gfp cDNA into a ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1016/0963-6897(95)02026-8
更新日期:1996-05-01 00:00:00
abstract::There is still a lack of sufficient research on the mechanism behind neurogenic bladder (NB) treatment. The aim of this study was to explore the effect of overexpressed stromal cell-derived factor-1 (SDF-1) secreted by engineered immortalized mesenchymal stem cells (imMSCs) on the NB. In this study, primary bone marro...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689720902466
更新日期:2020-01-01 00:00:00
abstract::Near infrared fluorescence (NIRF) optical imaging is a technique particularly powerful when studying in vivo processes at the molecular level in preclinical animal models. We recently demonstrated liver irradiation under the additional stimulus of partial hepatectomy as being an effective primer in the rat liver repop...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:
更新日期:2009-01-01 00:00:00
abstract::Multilineage-differentiating stress-enduring (Muse) cells are endogenous pluripotent stem cells that can be isolated based on stage-specific embryonic antigen-3 (SSEA-3), a pluripotent stem cell-surface marker. However, their capacities for survival, neurotrophic factor secretion, and neuronal and glial differentiatio...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689719863809
更新日期:2019-09-01 00:00:00
abstract::Testicular germ cell tumors (TGCTs) are highly prevalent in young men aged 20-40 years and are one of the most common lethal solid tumors in men of this age. Due to the current unclear mechanism of tumor development, there is a lack of effective treatment, and therefore in-depth research of the molecular mechanism of ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689720946653
更新日期:2020-01-01 00:00:00
abstract::Multiple system atrophy (MSA) is a neurodegenerative disorder that occurs sporadically and causes parkinsonism, cerebellar, autonomic, urinary, and pyramidal dysfunction in many combinations. Progressive L-dopa-unresponsive parkinsonism due to underlying striatonigral degeneration dominates the clinical syndrome in th...
journal_title:Cell transplantation
pub_type: 杂志文章,评审
doi:10.1177/096368970000900213
更新日期:2000-03-01 00:00:00
abstract::Alzheimer's disease (AD) is a type of neurodegenerative disorder and the most common form of dementia. MicroRNA (miRNA) has been shown to play a role in various diseases, including AD. It also has been reported to regulate autophagy. We extracted miRNA from blood samples and constructed an miRNA-101a lentivirus vector...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689719857085
更新日期:2019-08-01 00:00:00
abstract::Multiple sclerosis (MS) is a severe debilitating disorder characterized by progressive demyelination and axonal damage of the central nervous system (CNS). Current therapies for MS inhibit the immune response and demonstrate reasonable benefits if applied during the early phase of relapsing–remitting MS (RRMS) while t...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368912X657404
更新日期:2013-01-01 00:00:00
abstract::The anatomical specificity of axon growth from fetal pig septal xenografts was studied by transplanting septal cells from E30-35 pig fetuses into cholinergic deafferented (192-IgG-saporin-infused) rats or into aged rats (> 18 months). Cell suspensions (100,000 cells/microl) were injected bilaterally into the dorsal an...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368979900800104
更新日期:1999-01-01 00:00:00
abstract:BACKGROUND:Extracellular vesicles (EVs) released from mesenchymal stem/stromal cells (MSCs) mediate their paracrine effect, but their efficacy to protect the microcirculation of the kidney is unknown. Using a novel swine model of unilateral renovascular disease (RVD) complicated by metabolic syndrome (MetS), we tested ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689718780942
更新日期:2018-07-01 00:00:00
abstract::Hepatocyte transplantation has been proposed as a technique for bridging patients to whole-organ transplantation, for providing metabolic support during liver failure, and for replacing whole-organ transplantation in certain metabolic liver diseases. Assessment of hepatocyte engraftment has been difficult to measure, ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000004783983945
更新日期:2004-01-01 00:00:00
abstract::Transplantation of human cells after isolation and culture has become an important alternative for treatment of acute or chronic skin wounds. To increase the efficacy and reduce cost for transplantation of skin cells, more efficient and accurate techniques for evaluation of cell proliferation are needed. Hemocytometer...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000006783982115
更新日期:2006-01-01 00:00:00
abstract::Osteoarthritis (OA) is degenerative disease, leading to pain and functional disability. It is reported that polydeoxyribonucleotide (PDRN) is a suitable therapy for OA. However, the therapeutic mechanisms of PDRN in OA are not fully understood. To investigate the effect of PDRN in an in vitro model of OA, interleukin ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689718804130
更新日期:2018-11-01 00:00:00
abstract::The therapeutic use of stem cells to treat diseases and injuries is a promising tool in regenerative medicine. The umbilical cord provides a rich source of stem cells; we have previously reported a population of stem cells isolated from Wharton's jelly. In this report, we aimed to isolate a novel cell population that ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368912X655064
更新日期:2013-01-01 00:00:00
abstract::It is widely believed that human embryonic stem (huES) cells may represent a valid alternative to donor pancreata as a source of islets for transplantation. Much is known about the transcription factors whose sequential activation results in the generation of islets during pancreatic development. This knowledge has be...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000006783982359
更新日期:2006-01-01 00:00:00
abstract::Membrane integrity fluorescent staining is used routinely to evaluate islet viability. Results are used as one of the determining factors in islet product release criteria, and are used to assess the efficacy of different culture conditions. Recently, it has been observed that there is variation in the viability stain...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000004783983701
更新日期:2004-01-01 00:00:00
abstract::This invited concise review was written for the special issue of Cell Transplantation to celebrate the 25th anniversary of the American Society for Neural Therapy and Repair (ASNTR). I aimed to present a succinct summary of two interweaved lines of research work carried out by my team members and collaborators over th...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689719850088
更新日期:2019-04-01 00:00:00
abstract::The aim of this study was to evaluate the qualitative change in reparative cartilage after autologous chondrocyte implantation (ACI). Ten knees of 10 patients were studied. The signal intensities of reparative and normal cartilage were evaluated by fat-suppressed three-dimensional spoiled-gradient recalled (FS 3D-SPGR...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000005783982666
更新日期:2005-01-01 00:00:00
abstract::The hypogonadal (HPG) mouse is a mutant that lacks a functional gonadotropin-releasing hormone (GnRH) gene. In this study, female HPG mice received bilateral intrahypothalamic implants of an immortalized GnRH-secreting cell line (GT1-7). Nine mice were tested 42- 65 days after implantation to determine whether these c...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368979300200309
更新日期:1993-05-01 00:00:00
abstract::Diabetes affects millions of people worldwide, and β-cell replacement is one of the promising new strategies for treatment. Induced pluripotent stem cells (iPSCs) can differentiate into any cell type, including pancreatic β cells, providing a potential treatment for diabetes. However, the molecular mechanisms underlyi...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689717720281
更新日期:2017-08-01 00:00:00
abstract::Exenatide is an analog of the incretin hormone glucagon-like peptide (GLP-1) that is used for the treatment of T2D for their metabolic effects. In addition to its insulinotropic effects, exenatide increases functional islet mass and improves their survival. Improved outcomes have been reported in recent clinical isle...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368911X576027
更新日期:2012-01-01 00:00:00
abstract::Acute kidney injury (AKI) is a major clinical problem that still has no established treatment. We investigated the efficacy of cultured human peripheral blood mononuclear cells (PBMNCs) for AKI. Ischemia/reperfusion injury (IRI) was used to induce AKI in male nonobese diabetic (NOD/severe combined immunodeficiency) mi...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689717753186
更新日期:2018-03-01 00:00:00
abstract::Several previous studies, suggesting the potential use of embryonic xenografts in the treatment of neurological disorders, indicate that neural growth and axonal guidance factors may function across species. In this light, blocks of fetal porcine neocortex were grafted into small cortical lesion cavities made in newbo...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000003108747343
更新日期:2003-01-01 00:00:00
abstract::The use of biomaterials has been demonstrated as a viable strategy to promote cell survival and cardiac repair. However, limitations on combinational cell-biomaterial therapies exist, as cellular behavior is influenced by the microenvironment and physical characteristics of the material. Among the different scaffolds ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368915X689794
更新日期:2016-01-01 00:00:00
abstract::Acute myocardial infarction (AMI) results in ischemic damage and death of cardiomyocytes and loss of vasculature. Stem cell therapy has emerged as a potentially promising strategy for maximizing cardiac function following ischemic injury. Issues of cell source, delivery, and quantification of response have challenged...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368911X603657
更新日期:2012-01-01 00:00:00
abstract::Stem cell transplants into damaged myocardium may have the potential to improve cardiac function. We investigated the safety of transplanting unmanipulated autologous bone marrow into infarcted myocardium of patients undergoing coronary bypass surgery and assessed its efficacy to improve cardiac function. Fourteen pat...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000004772664842
更新日期:2004-01-01 00:00:00
abstract::Three-dimensional culture procedures have attracted attention in various fields of cell biology. A newly developed cell array assisted in the formation of hepatocyte spheroids by two innovations: 1) micropatterning by a hydrophilic polymer, and 2) the use of bovine carotid artery-derived HH cells as feeder cells. The ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368970901805-624
更新日期:2009-01-01 00:00:00