Abstract:
:Sepsis is a life-threatening disease that results in excessive stimulation of the host's immune cells. In the animal study, the purpose was to investigate the roles of fresh frozen plasma (FFP) transfusion in shaping the CD4+ T lymphocytes immune response through modulating the secreted exosome protein Galectin-9 in mice with severe sepsis. By using Western blot analysis, we first identified that the protein Galectin-9 is highly accumulated in the blood plasma of severe sepsis mice, and with transmission electron microscopy (TEM) and protein analysis, we found that Galectin-9 is a secreted exosome protein. Thereafter, we treated the severe sepsis mice with the antibiotic Cefuroxime Axetil; one group of mice received FFP transfusion and the other group of mice received normal saline. Surprisingly, the FFP transfusion reduced the secretion of exosome protein Galectin-9 and there was crosstalking between the exosome protein Galectin-9 and CD4+ T lymphocytes in mice with severe sepsis. Results showed that the proliferation of T helper (Th) cells (Th1 and Th17) was promoted, and regulatory T (Treg) cells' maintenance was inhibited in the sepsis mice after receiving FFP transfusion. Correspondingly, this immune reprogrammed activity shaped the inflammatory cytokine secretion with an increase in the interleukin (IL)-1β, IL-6, and interferon-gamma levels, while it decreased IL-10 levels. Taken together, it was suggested that FFP transfusion promoted reprogramming of CD4+ T lymphocytes' immune response through inhibiting the secretion of exosome protein Galectin-9 in mice with severe sepsis to relieve immunosuppression.
journal_name
Cell Transplantjournal_title
Cell transplantationauthors
Zhang L,Zhang JP,Liu Y,Wang H,Cheng Y,Wang JH,Zhang WJ,Li ZZ,Guo JRdoi
10.1177/0963689720947347subject
Has Abstractpub_date
2020-01-01 00:00:00pages
963689720947347eissn
0963-6897issn
1555-3892journal_volume
29pub_type
杂志文章abstract::Somatic stem cells can be isolated from a variety of sources. Although some studies have suggested that somatic stem cells may represent a cell population that is very similar to embryonic stem (ES) cells, it remains unclear whether somatic stem cells retain the potential to differentiate into any cell type derived fr...
journal_title:Cell transplantation
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abstract::Critical limb ischemia in diabetic patients is associated with high rates of morbidity and mortality. Suboptimal responses to the available medical and surgical treatments are common in these patients, who also demonstrate limited vascular homeostasis. Neovasculogenesis induced by stem cell therapy could be a useful ...
journal_title:Cell transplantation
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journal_title:Cell transplantation
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journal_title:Cell transplantation
pub_type: 杂志文章,评审
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journal_title:Cell transplantation
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journal_title:Cell transplantation
pub_type: 杂志文章
doi:
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journal_title:Cell transplantation
pub_type: 杂志文章
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journal_title:Cell transplantation
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abstract::The major limitation of nonhuman primate (NHP) embryonic stem (ES) cell research is inefficient genetic modification and limited knowledge of differentiation mechanisms. A genetically modified NHP-ES cell with biomarkers, such as green fluorescent protein (GFP), that allow noninvasive monitoring of transgenic cells, i...
journal_title:Cell transplantation
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journal_title:Cell transplantation
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journal_title:Cell transplantation
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doi:10.3727/096368912X657404
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journal_title:Cell transplantation
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doi:10.1177/0963689719857085
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journal_title:Cell transplantation
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doi:10.3727/096368913X670174
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doi:10.1177/0963689719831707
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journal_title:Cell transplantation
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journal_title:Cell transplantation
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journal_title:Cell transplantation
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