Abstract：
:Critical limb ischemia in diabetic patients is associated with high rates of morbidity and mortality. Suboptimal responses to the available medical and surgical treatments are common in these patients, who also demonstrate limited vascular homeostasis. Neovasculogenesis induced by stem cell therapy could be a useful approach for these patients. Neovasculogenesis and clinical improvement were compared at baseline and at 3 and 12 months after autologous bone marrow-derived mononuclear cell (BMMNC) transplantation in diabetic patients with peripheral artery disease. We conducted a prospective study to evaluate the safety and efficacy of intra-arterial administration of autologous BMMNCs (100-400 × 10(6) cells) in 20 diabetic patients with severe below-the-knee arterial ischemia. Although the time course of clinical effects differed among patients, after 12 months of follow-up all patients presented a notable improvement in the Rutherford-Becker classification, the University of Texas diabetic wound scales, and the Ankle-Brachial Index in the target limb. The clinical outcome was consistent with neovasculogenesis, which was assessed at 3 months by digital subtraction angiography and quantified by MetaMorph software. Unfortunately, local cell therapy in the target limb had no beneficial effect on the high mortality rate in these patients. In diabetic patients with critical limb ischemia, intra-arterial perfusion of BMMNCs is a safe procedure that generates a significant increase in the vascular network in ischemic areas and promotes remarkable clinical improvement.
journal_name
Cell Transplantjournal_title
Cell transplantationauthors
Ruiz-Salmeron R,de la Cuesta-Diaz A,Constantino-Bermejo M,Pérez-Camacho I,Marcos-Sánchez F,Hmadcha A,Soria Bdoi
10.3727/096368910X0177subject
Has Abstractpub_date
2011-01-01 00:00:00pages
1629-39issue
10eissn
0963-6897issn
1555-3892journal_volume
20pub_type
杂志文章abstract：:The major limitation of nonhuman primate (NHP) embryonic stem (ES) cell research is inefficient genetic modification and limited knowledge of differentiation mechanisms. A genetically modified NHP-ES cell with biomarkers, such as green fluorescent protein (GFP), that allow noninvasive monitoring of transgenic cells, i...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/000000002783985350
更新日期：2002-01-01 00:00:00
abstract：:Adipose tissue contains a mesenchymal stem cell (MSC) population known as adipose-derived stem cells (ASCs) capable of differentiating into different cell types. Our aim was to induce hepatic transdifferentiation of ASCs by sequential exposure to several combinations of cytokines, growth factors, and hormones. The mos...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368909X12483162197321
更新日期：2009-01-01 00:00:00
abstract：:Regenerative medicine is a new field primarily based on the concept of transplanting exogenous or stimulating endogenous stem cells to generate biological substitutes and improve tissue functions. Recently, amnion-derived cells have been reported to have multipotent differentiation ability, and these cells have attrac...
journal_title：Cell transplantation
pub_type： 杂志文章,评审
doi：10.3727/096368909788341243
更新日期：2009-01-01 00:00:00
abstract：:Despite recent advances and promising results in children, liver cell transplantation (LCT) should still be regarded as an experimental therapy. Several substantial complications are known from animal studies and individual patients. However, safety data on liver cell infusion in children are scarce. We used LCT in fo...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368909X485058
更新日期：2010-01-01 00:00:00
abstract：:The choice of enzyme blend is critical for successful islet isolation. Islet yield, viability, integrity, and function are important factors that influence the outcome of islet transplantation. Liberase HI has been used as a standard enzyme for pancreas digestion and has successfully produced islets that reversed dia...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368911X623826
更新日期：2012-01-01 00:00:00
abstract：:Attaining consistent robust engraftment in the structurally normal liver is an obstacle for cellular transplantation. Most experimental approaches to increase transplanted cells' engraftment involve recipient-centered deleterious methods such as partial hepatectomy or irradiation which may be unsuitable in the clinic....
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1177/0963689717737320
更新日期：2017-12-01 00:00:00
abstract：:Hematopoietic stem cells (HSCs) migrate to injury sites and aid in tissue repair. However, clinical success is poor and is partially due to limited HSC recruitment. We hypothesized that HSC pretreatment with H2O2 would enhance their recruitment to injured gut. As HSCs are rare cells, the number of primary cells obtain...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368912X653192
更新日期：2013-01-01 00:00:00
abstract：:In cartilage tissue engineering using stem cells, it is important to stimulate proliferation and control the differentiation of stem cells to specific lineages. Here we reported a combined technique for articular cartilage repair, consisting of bone marrow mesenchymal stem cells (BMMSCs) transfected with connective ti...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368913X669770
更新日期：2014-04-01 00:00:00
abstract：:The main purpose of this work has been to establish a new culturing technique to improve the chondrogenic commitment of isolated adult human chondrocytes, with the aim of being used during cell-based therapies or tissue engineering strategies. By using a rather novel technique to generate scaffold-free three-dimension...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368908786576499
更新日期：2008-01-01 00:00:00
abstract：:The midbrain inferior colliculus (IC), a major integrating center for auditory processing, provides a model for structural, functional, and behavioral recovery. The present study examined the role of IC in spatial sound detection, and the effects of neural transplantation in sparing of behavioral performance. Hooded r...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1016/0963-6897(95)02036-5
更新日期：1996-03-01 00:00:00
abstract：:During the isolation of human islets of Langerhans the digest has repeated direct contact with the ambient atmosphere. In order to fulfill GMP requirements in clinical applications, the entire cell preparation must be performed in clean room facilities. We hypothesized that the use of a closed system, which avoids the...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/000000007783465091
更新日期：2007-01-01 00:00:00
abstract：BACKGROUND:Extracellular vesicles (EVs) released from mesenchymal stem/stromal cells (MSCs) mediate their paracrine effect, but their efficacy to protect the microcirculation of the kidney is unknown. Using a novel swine model of unilateral renovascular disease (RVD) complicated by metabolic syndrome (MetS), we tested ...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1177/0963689718780942
更新日期：2018-07-01 00:00:00
abstract：:Protocols of donor bone marrow infusion for tolerance induction are receiving increasing attention in clinical trials of organ allotransplantation. We report pulmonary embolism with bone fragments following vertebral body marrow infusion in a recipient of a liver and intestinal transplant. Even though pulmonary emboli...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1016/0963-6897(96)00026-7
更新日期：1996-07-01 00:00:00
abstract：:The anatomical specificity of axon growth from fetal pig septal xenografts was studied by transplanting septal cells from E30-35 pig fetuses into cholinergic deafferented (192-IgG-saporin-infused) rats or into aged rats (> 18 months). Cell suspensions (100,000 cells/microl) were injected bilaterally into the dorsal an...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1177/096368979900800104
更新日期：1999-01-01 00:00:00
abstract：:Epithelial wound repair assures the recovery of the epithelial barrier after wounding. During wound healing epithelial cells migrate to cover the wound surface. The presented experiments were carried out to compare the migration of human keratinocytes from primary and secondary culture on polystyrene, collagen, and fi...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：:Although hepatic stem cells are believed to exist and play a critical role in developing and regenerating liver, little is known about their cell surface specificity or differentiation capabilities. To make prospective studies of hepatic stem cells possible, we established an in vitro culture system for identification...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：:Pancreatic beta cell lines may offer an abundant source of cells for beta-cell replacement in type I diabetes. Using regulatory elements of the bacterial tetracycline (tet) operon for conditional expression of SV40 T antigen oncoprotein in transgenic mouse beta cells, we have shown that reversible immortalization is a...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：:Potential labels for identifying embryonic raphe neurons and a clonal, neuronally differentiating, raphe-derived cell line, RN33B, in CNS transplantation studies were tested by first characterizing the labels in vitro. The labels that were tested included 4',6-diamidino-2-phenylindole hydrochloride, 1,1'-dioctadecyl-3...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1177/096368979300200207
更新日期：1993-03-01 00:00:00
abstract：:Antibodies to the ligand for CD40 (CD154) have been shown to exert profound effects on the development of cell-mediated immune responses in mice. The present study shows that an antibody to human CD154 (hCD40L) inhibits in vivo Tetanus toxoid (TT) specific secondary antibody responses in hu-PBL-scid mice, as well as t...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1016/s0963-6897(97)00113-9
更新日期：1998-01-01 00:00:00
abstract：:Bisphosphonates (BPs) are widely used to treat several metabolic and oncological diseases affecting the skeletal system. Despite BPs' well-known therapeutic potential, they also displayed important side effects, among which is BPs-related osteonecrosis of the jaw, by targeting osteoclast activities, osteoblast, and os...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1177/0963689720948497
更新日期：2020-01-01 00:00:00
abstract：:Clinically, many candidates for islet transplantation are already immunized, which increases their risk of graft rejection. Encapsulation of pancreatic islets using the TheraCyte™ device has been shown to protect against allograft rejection in nonimmunized recipients. However, the capacity of the TheraCyte™ device to ...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368912X657486
更新日期：2013-01-01 00:00:00
abstract：:Mouse embryonal carcinoma cell line P19 could be differentiated to neurons or glial cells by appropriate induction. We introduced the lacZ gene driven by a calmodulin promoter into P19 cells, and cloned the transfectant CaM-Z that showed the strong expression of lacZ gene after the induction of differentiation. CaM-Z ...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1016/0963-6897(96)00030-9
更新日期：1996-09-01 00:00:00
abstract：:The aim of this work was to determine the effect of nicotine desensitization on dopamine (DA) release in the dorsal striatum and shell of the nucleus accumbens (NAc) from brain slices. In vitro fast-scan cyclic voltammetry analysis was used to evaluate dopamine release in the dorsal striatum and the NAc shell of Sprag...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1177/0963689718775382
更新日期：2019-03-01 00:00:00
abstract：:Changes in morphology, metabolism, myosin heavy chain gene expression, and functional performances in damaged rabbit muscles with or without transplantation of primary satellite cells were investigated. For this purpose, we damaged bilaterally the fast muscle tibialis anterior (TA) with either 1.5 or 2.6 ml cardiotoxi...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：
更新日期：2002-01-01 00:00:00
abstract：:In previous work, we engineered functional cell sheets using bone marrow-derived mesenchymal stem cells (BM-MSCs) to promote islet graft survival. In the present study, we hypothesized that a cell sheet using dermal fibroblasts could be an alternative to MSCs, and then we aimed to evaluate the effects of this cell she...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368916X690854
更新日期：2016-01-01 00:00:00
abstract：:We have recently reported that small-sized bone marrow cells (BMCs) isolated by counterflow centrifugal elutriation and depleted of lineage markers (Fr25lin(-)) have the capacity to differentiate and contribute to regeneration of injured islets. In this study, we assess some of the characteristics of these cells compa...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368913X676899
更新日期：2015-01-01 00:00:00
abstract：:Wharton's jelly-derived mesenchymal stromal cells (WJ-MSCs) have distinct immunomodulatory and protective effects against kidney, liver, or heart injury. Limited studies have shown that WJ-MSCs attenuates oxygen-glucose deprivation-mediated inflammation in hippocampal slices. The neuroprotective effect of intracerebra...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1177/0963689718802754
更新日期：2018-11-01 00:00:00
abstract：:ZNF281 is one of the core transcription factors in embryonic stem cells (ESCs) and has activation and repression roles in the transcription of ESC genes. A known target molecule of Zfp281 (the mouse homologue of ZNF281) is Nanog. However, NANOG is not expressed in most human multipotent stem cells (hMSCs). Here, we in...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368912X654948
更新日期：2013-01-01 00:00:00
abstract：:Transplantation therapies aimed at repairing neurodegenerative and neuropathological conditions of the central nervous system (CNS) have utilized and tested a variety of cell candidates, each with its own unique set of advantages and disadvantages. The use and popularity of each cell type is guided by a number of fact...
journal_title：Cell transplantation
pub_type： 杂志文章,评审
doi：10.3727/096368912X655208
更新日期：2013-01-01 00:00:00
abstract：:Mesenchymal stem cells (MSCs) hold great promise for bone regeneration. However, the power of mesenchymal stem cells has not been applied to structural bone allografts in clinical practice. This study designed a new strategy to enhance the efficiency of allografts for segmental bone regeneration. Isolated MSCs were cu...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368909788809839
更新日期：2009-01-01 00:00:00
