Abstract:
:Mesenchymal stem cells (MSCs) hold great promise for bone regeneration. However, the power of mesenchymal stem cells has not been applied to structural bone allografts in clinical practice. This study designed a new strategy to enhance the efficiency of allografts for segmental bone regeneration. Isolated MSCs were cultured to form a cell sheet. The MSC sheet was then wrapped onto structural allografts. The assembled structures were cultured in vitro to evaluate the differentiation potential of MSC sheet. The assembled structures were implanted subcutaneously into nude mice as well as into the segmental radius defect of rabbits to investigate the efficiency of MSC sheets to repopulate allografts for bone repair. MSC sheets, upon assembling on bone grafts, showed similar differentiation properties to the in situ periosteum in vitro. After implantation the MSC sheets accelerated the repopulation of bone grafts in nude mice. Moreover, MSC sheets induced thicker cortical bone formation and more efficient graft-to-bone end fusion at the segmental bone defects in rabbits. This study thus presented a novel, more efficient, and practical strategy for large weight-bearing bone reconstruction by using MSC sheets to deliver large number of MSCs to repopulate the bone allografts.
journal_name
Cell Transplantjournal_title
Cell transplantationauthors
Zou XH,Cai HX,Yin Z,Chen X,Jiang YZ,Hu H,Ouyang HWdoi
10.3727/096368909788809839subject
Has Abstractpub_date
2009-01-01 00:00:00pages
433-41issue
4eissn
0963-6897issn
1555-3892journal_volume
18pub_type
杂志文章abstract::Persistent corneal wounds result from numerous eye disorders, and to date, available treatments often fail to accelerate reepithelialization, the key initial step in wound healing. To speed reepithelialization, we explored a cell-transfer transplant method utilizing polydimethylsiloxane (PDMS) contact lenses to delive...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000005783982783
更新日期:2005-01-01 00:00:00
abstract::Traumatic brain injury (TBI), a major cause of mortality and morbidity, affects 10 million people worldwide, with limited treatment options. We have previously shown that (-)-phenserine (Phen), an acetylcholinesterase inhibitor originally designed and tested in clinical phase III trials for Alzheimer's disease, can re...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689719854693
更新日期:2019-09-01 00:00:00
abstract::Bone marrow (BM) cells may interact with coronary endothelium and modulate coronary atherosclerosis. We investigated the time course of coronary luminal loss and changes in conductance after intracoronary injection of enriched hematopoietic BM stem cells in patients with previous myocardial infarction (MI). Among 24 p...
journal_title:Cell transplantation
pub_type: 杂志文章,随机对照试验
doi:10.3727/096368907783338244
更新日期:2007-01-01 00:00:00
abstract::Nerve growth factor (NGF) exerts both trophic (cell survival) and tropic (axonal growth-promoting) effects on several neuronal populations. In particular, its robust ability to prevent lesion-induced and spontaneous age-related basal forebrain cholinergic neuronal degeneration, and to promote mnemonic recovery, has su...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368970000900508
更新日期:2000-09-01 00:00:00
abstract::Oligodendrocyte (OL) replacement can be a promising strategy for spinal cord injury (SCI) repair. However, the poor posttransplantation survival and inhibitory properties to axonal regeneration are two major challenges that limit their use as donor cells for repair of CNS injuries. Therefore, strategies aimed at enhan...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368912X661373
更新日期:2013-01-01 00:00:00
abstract::The clinical consequences of acute liver failure are associated with high mortality. Intensive medical intervention is required to treat the symptoms of liver failure, including coagulopathy, metabolic instability, and encephalopathy. Providing temporary liver support with an extracorporeal liver assist device could s...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368970000900101
更新日期:2000-01-01 00:00:00
abstract::The main purpose of this work has been to establish a new culturing technique to improve the chondrogenic commitment of isolated adult human chondrocytes, with the aim of being used during cell-based therapies or tissue engineering strategies. By using a rather novel technique to generate scaffold-free three-dimension...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368908786576499
更新日期:2008-01-01 00:00:00
abstract::Epithelial wound repair assures the recovery of the epithelial barrier after wounding. During wound healing epithelial cells migrate to cover the wound surface. The presented experiments were carried out to compare the migration of human keratinocytes from primary and secondary culture on polystyrene, collagen, and fi...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
abstract::Several types of hair loss result from the inability of hair follicles to initiate the anagen phase of the hair regeneration cycle. Modulating signaling pathways in the hair follicle niche can stimulate entry into the anagen phase. Despite much effort, stem cell-based or pharmacological therapies to activate the hair ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368916X691466
更新日期:2016-01-01 00:00:00
abstract::We examined a possible protective effect of the nonpeptidyl low molecular weight radical scavenger IAC [bis(1-hydroxy-2,2,6,6-tetramethyl-4-piperidinyl)decanedioate di-hydrochloride] on isolated human islet cells against isolation and culture oxidative stress. Islets isolated from pancreases of nondiabetic multiorgan ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368908787236639
更新日期:2008-01-01 00:00:00
abstract::A new extracorporeal bioartificial liver using alginate-entrapped hepatocytes was developed and evaluated for its ability to correct the lack of bilirubin conjugation in the Gunn rat. Hepatocytes were harvested from Sprague-Dawley rats by the two-step collagenase perfusion method and then immobilized in Ca(++)-alginat...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368979300200603
更新日期:1993-11-01 00:00:00
abstract::The prevalence of HIV-associated neurocognitive disorders (HAND) remains high despite combination antiretroviral therapy (cART). There is evidence that neural stem cells (NSCs) can migrate to sites of brain injury such as those caused by inflammation and oxidative stress, which are pathological features of HAND. Thus,...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368916X691457
更新日期:2016-11-01 00:00:00
abstract::A large proportion of islets are lost after transplantation partly due to a lack of functional vasculature. Islets revascularize from host tissue but the process takes up to 2 weeks and has been suggested to result in reduced vascular density in engrafted islets. We describe a method for observing and quantifying the ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000007783464993
更新日期:2007-01-01 00:00:00
abstract::Procurement of multipotential neuroglial stem cells is possible with the addition of epidermal growth factor (EGF). Stem cells will differentiate into neurons and glia upon the removal of EGF from the culture medium. We have previously characterized the neuronal differentiation of stem cells derived from long-term cul...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1016/0963-6897(95)02043-8
更新日期:1996-03-01 00:00:00
abstract::Human embryonic stem (hES) cells have the ability to differentiate into a variety of different cell lineages and potentially provide a source of differentiated cells for many therapeutic uses. Here we investigated an efficient method of hepatic differentiation from hES cells. A human ES cell line, KhES-1, was used and...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000006783981305
更新日期:2006-01-01 00:00:00
abstract::Circular RNAs (circRNAs) could sponge micro-RNAs (miRNAs) to regulate tumor progression of hepatocellular carcinoma (HCC). Hsa_circ_104566 contributes to papillary thyroid carcinoma progression. However, the tumorigenic mechanism of hsa_circ_104566 on HCC remains enigmatic. The role of hsa_circ_104566 on HCC was there...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689720963948
更新日期:2020-01-01 00:00:00
abstract::Electrostimulation represents a well-known trophic factor for different tissues. In vitro electrostimulation of non-stem and stem cells induces myogenic predifferentiation and may be a powerful tool to generate cells with the capacity to respond to local areas of injury. We evaluated the effects of in vivo electrostim...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368912X652977
更新日期:2013-01-01 00:00:00
abstract::Persistent fetal vasculature (PFV) is a potentially serious developmental anomaly in human eyes, which results from a failure of the primary vitreous and the hyaloid vascular systems to regress during development. Recent findings from our laboratory indicate that fibrovascular membranes harvested from subjects with P...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368912X647153
更新日期:2012-01-01 00:00:00
abstract::Major skin burns are difficult to treat. Patients often require special care and long-term hospitalization. Besides specific complications associated with the wounds themselves, there may be impairment of the immune system and of other organs. Mesenchymal stromal cells (MSCs) are a recent therapeutic alternative to tr...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368915X688128
更新日期:2016-01-01 00:00:00
abstract::In human neonates, immature GABAergic interneurons are markedly affected by an excitotoxic insult. While in adults the interest of cell transplantation has been demonstrated in several neurological disorders, few data are available regarding the immature brain. The low survival rate constitutes a strong limitation in ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368913X670174
更新日期:2014-01-01 00:00:00
abstract::Spinal cord injury (SCI) causes loss of neural functions below the level of the lesion due to interruption of spinal pathways and secondary neurodegenerative processes. The transplant of neural stem cells (NSCs) is a promising approach for the repair of SCI. Reprogramming of adult somatic cells into induced pluripoten...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368916X691312
更新日期:2016-10-01 00:00:00
abstract::Gene therapy as well as methods capable of returning cells to a pluripotent state (iPS) have enabled the correction of genetic deficiencies in syngenic adult progenitors, reducing the need for immunosuppression in cell therapy approaches. However, in diseases involving mutations that lead to the complete lack of a pr...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368910X545068
更新日期:2011-01-01 00:00:00
abstract::The nonobese diabetic (NOD) mouse model of type 1 diabetes (T1D) was discovered by coincidence in the 1980s and has since been widely used in the investigation of T1D and diabetic complications. The current in vivo study was originally designed to prospectively assess whether hyperglycemia onset is associated with phy...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689720939127
更新日期:2020-01-01 00:00:00
abstract::Cell therapy is a promising treatment modality to improve heart function in acute myocardial infarction. However, the mechanisms of action and the most suitable cell type have not been finally determined. We performed a study to compare the effects of mesenchymal stem cells (MSCs) harvested from different tissues on ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368911X627462
更新日期:2012-01-01 00:00:00
abstract::Ex vivo embryonic liver explant culture is a novel and attractive approach to obtain abundant hepatic and hematopoietic stem cells. Gene therapy of autologous hepatic and hematopoietic stem cells represents an alternative therapeutic approach to liver transplantation for genetic and metabolic disorders. In this study ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000001783986945
更新日期:2001-01-01 00:00:00
abstract::The biohybrid artificial pancreas is designed to enclose pancreatic tissues with a selectively permeable membrane that immunoisolates the graft from the host immune system, allowing those endocrine tissues to survive and control glucose metabolism for an extended period of time. The pancreatic B cell line MIN6 is esta...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1016/s0963-6897(97)00074-2
更新日期:1997-09-01 00:00:00
abstract::As a featured ocular inflammatory disease, autoimmune uveitis is the major cause of blindness in the clinic. Although current immunosuppressive regimens can alleviate the progression of autoimmune uveitis, they have serious side effects. Therefore, an alternative therapeutic strategy is urgently required. The present ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689718796196
更新日期:2018-10-01 00:00:00
abstract::The third-generation NOD/LtSz-scid/IL2Rγ(null) (NOD/SCID IL2Rγ(null)) mouse represents a significantly improved xenograft model allowing high levels of human leukocyte engraftment over extended follow up. One remaining limitation of this mouse model, however, is the low level of circulating human erythrocytes. We esta...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368910X314161
更新日期:2010-01-01 00:00:00
abstract::Mouse embryonal carcinoma cell line P19 could be differentiated to neurons or glial cells by appropriate induction. We introduced the lacZ gene driven by a calmodulin promoter into P19 cells, and cloned the transfectant CaM-Z that showed the strong expression of lacZ gene after the induction of differentiation. CaM-Z ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1016/0963-6897(96)00030-9
更新日期:1996-09-01 00:00:00
abstract::Posttransplant infusion of viable donor bone marrow cells (DBMC) has been shown in our previous studies to promote acceptance of incompatible kidney allografts in rhesus monkeys after treatment with polyclonal antithymocyte globulin to deplete peripheral T-lymphocytes. In this nonhuman primate model, the infusion of t...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1016/s0963-6897(97)00005-5
更新日期:1997-07-01 00:00:00