Abstract:
:Nerve growth factor (NGF) exerts both trophic (cell survival) and tropic (axonal growth-promoting) effects on several neuronal populations. In particular, its robust ability to prevent lesion-induced and spontaneous age-related basal forebrain cholinergic neuronal degeneration, and to promote mnemonic recovery, has suggested its potential use as a therapeutic agent in Alzheimer's disease. When infused intracerebroventricularly, however, NGF is associated with several adverse effects that make this delivery route impractical. The present study examined whether intraparenchymal infusions of NGF adjacent to cholinergic neuronal soma are an effective and well-tolerated means of providing NGF to degenerating cholinergic neurons. Cholinergic neuronal rescue together with axonal sprouting responses and local tissue damage in the brain were assessed in adult rats that underwent complete unilateral fornix transections, followed by intraparenchymal infusions of recombinant human NGF for a 2-week period. Intraparenchymal NGF infusions prevented the degeneration of 94.7+/-6.6% of basal forebrain cholinergic neurons compared to 21.7+/-2.6% in vehicle-infused animals (p < 0.0001). Cholinergic axons sprouted toward the intraparenchymal NGF source in an apparent gradient-dependent manner. Glial responses to intraparenchymal infusions were minimal, and no apparent toxic effects of the infusions were observed. Thus, when infused intraparenchymally, NGF rescues basal forebrain cholinergic neurons, alters the topography of axonal sprouting responses, and does not induce adverse affects over a 2-week infusion period. Intraparenchymal NGF delivery merits further study at longer term time points as a means of treating the cholinergic component of neuronal loss in Alzheimer's disease.
journal_name
Cell Transplantjournal_title
Cell transplantationauthors
Tuszynski MHdoi
10.1177/096368970000900508subject
Has Abstractpub_date
2000-09-01 00:00:00pages
629-36issue
5eissn
0963-6897issn
1555-3892journal_volume
9pub_type
杂志文章abstract::Experiments on spinal cord injury (SCI) have largely focused on the transplantation of stem cells into injured spinal cords for motor recovery while neglecting to investigate bladder dysfunction. The present study was performed to investigate the effect of B10 human mesenchymal stem cells (hMSCs) directly transplanted...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368914X682125
更新日期:2015-01-01 00:00:00
abstract::As an alternative to drug immunosuppression, attempts at inducing donor-specific tolerance by intrathymic (IT) inoculations to transplant recipient of donor origin alloantigenic products has proven very promising. Using fiber optic thoracoscopy, a technique for the study of this phenomena was developed for the dog. We...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368979400300413
更新日期:1994-07-01 00:00:00
abstract::As a putative marker of cerebral small vessel disease, cerebral microbleeds (CMBs) have been associated with vascular cognitive impairment. Both iron accumulation and amyloid protein precursor (APP) dysregulation are recognized as pathological hallmarks underlying the progression of CMBs, but their cross-talk is not y...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689719831707
更新日期:2019-08-01 00:00:00
abstract::Gene therapy as well as methods capable of returning cells to a pluripotent state (iPS) have enabled the correction of genetic deficiencies in syngenic adult progenitors, reducing the need for immunosuppression in cell therapy approaches. However, in diseases involving mutations that lead to the complete lack of a pr...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368910X545068
更新日期:2011-01-01 00:00:00
abstract::Recently, we described a diffusion chamber for a bioartificial endocrine pancreas (Bio-AEP). Pancreatic islet cells in the Bio-AEP device were isolated from the immune system of the host by an artificial barrier, while nutrients, electrolytes, oxygen, and bioactive secretory products were exchanged across this barrier...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368979900800409
更新日期:1999-07-01 00:00:00
abstract::Schwann cells naturally support axonal regeneration after injury in the peripheral nervous system, and have also shown a significant, albeit limited, ability to support axonal growth and remyelination after grafting to the central nervous system (CNS). It is possible that Schwann cell-induced axonal growth in the CNS ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1016/s0963-6897(97)00166-8
更新日期:1998-03-01 00:00:00
abstract::Cell therapy is a promising treatment modality to improve heart function in acute myocardial infarction. However, the mechanisms of action and the most suitable cell type have not been finally determined. We performed a study to compare the effects of mesenchymal stem cells (MSCs) harvested from different tissues on ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368911X627462
更新日期:2012-01-01 00:00:00
abstract::Cardiovascular diseases are related to many risk factors, such as diabetes, high blood pressure, smoking, and obesity. Myocardial infarction (MI), a cardiovascular disease, is the most common cause of cardiomyocyte death. In MI, hypoxia induces cardiomyocyte apoptosis; in particular, diabetes combined with MI has a sy...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368915X686995
更新日期:2015-01-01 00:00:00
abstract::During the past 15 years, a variety of peptides, known as protein transduction domains (PTDs), or cell-penetrating peptides (CPPs), have been characterized for their ability to translocate into live cells. There are now numerous examples of biologically active full-length proteins and peptides that have been successfu...
journal_title:Cell transplantation
pub_type: 杂志文章,评审
doi:10.3727/096368910X508744
更新日期:2010-01-01 00:00:00
abstract::Periosteal autografts have the potential to regenerate articular cartilage defects, but this potential is limited by the patient's age. Allograft transplantation from a young donor to an older recipient might bypass this limitation. The effect of the time delay, between death and harvesting of a periosteal graft, on t...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368979900800607
更新日期:1999-11-01 00:00:00
abstract::A human embryonic stem cell (HESC) line, H1, was studied after differentiation to a dopaminergic phenotype in vitro in order to carry out in vivo studies in Parkinsonian monkeys. To identify morphological characteristics of transplanted donor cells, HESCs were transfected with a GFP lentiviral vector. Gene expression ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368913X664865
更新日期:2014-01-01 00:00:00
abstract:REGISTRATION NUMBER OF CLINICAL TRIALS:ChiCTR1900021317. ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689720931428
更新日期:2020-01-01 00:00:00
abstract::Recent advances in tissue engineering technologies have highlighted the ability to create functional liver systems using isolated hepatocytes in vivo. Considering the serious shortage of donor livers that can be used for hepatocyte isolation, it has remained imperative to establish a hepatocyte propagation protocol t...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368911X605330
更新日期:2012-01-01 00:00:00
abstract::In adults, hematopoiesis takes places in the bone marrow, where specialized niches containing mesenchymal nonhematopoietic cells (stroma) harbor the hematopoietic stem cell (HSC). These niches are responsible and essential for the maintenance of HSCs. Attempts to expand HSCs fail to keep the general properties of ste...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368912X636993
更新日期:2012-01-01 00:00:00
abstract::Cord blood is an attractive cell source in regenerative medicine and represents an alternative to bone marrow. The aim of this study was to investigate whether human umbilical cord blood mononuclear (HUCBM) cells might be valuable in hepatic regenerative medicine. HUCBM cells differentiated in vitro into hepatocytes, ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368908786516837
更新日期:2008-01-01 00:00:00
abstract::One of the serious obstacles of the aortopathies research is a considerable shortage of human aortic smooth muscle cells (SMCs), which can be used to model the disease. SMC in most cases come from the whole aorta of transplant donors, which are rather difficult to access. In the course of coronary artery bypass graft ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689717721226
更新日期:2017-10-01 00:00:00
abstract::Multiple system atrophy (MSA) is a neurodegenerative disorder that occurs sporadically and causes parkinsonism, cerebellar, autonomic, urinary, and pyramidal dysfunction in many combinations. Progressive L-dopa-unresponsive parkinsonism due to underlying striatonigral degeneration dominates the clinical syndrome in th...
journal_title:Cell transplantation
pub_type: 杂志文章,评审
doi:10.1177/096368970000900213
更新日期:2000-03-01 00:00:00
abstract::"Proof-of-principle" that cell replacement therapy works for neurodegeneration has been reported, but only using donor cells collected from fetal brain tissue obtained from surgical terminations of pregnancy. Surgical terminations of pregnancy represent an increasingly limited supply of donor cells due to the tendenc...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368910X546580
更新日期:2011-01-01 00:00:00
abstract::Rare cells are present in human umbilical cord blood that do not express the hematopoietic marker CD45 and in culture do not produce cells of hematopoietic lineage. These umbilical cord multipotent stem cells (UC-MC) behave as multilineage progenitor cells (stem cells) and can be expanded in tissue culture. Exposure t...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096020197390022
更新日期:2002-04-01 00:00:00
abstract::In previous work, we engineered functional cell sheets using bone marrow-derived mesenchymal stem cells (BM-MSCs) to promote islet graft survival. In the present study, we hypothesized that a cell sheet using dermal fibroblasts could be an alternative to MSCs, and then we aimed to evaluate the effects of this cell she...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368916X690854
更新日期:2016-01-01 00:00:00
abstract::We have recently reported that small-sized bone marrow cells (BMCs) isolated by counterflow centrifugal elutriation and depleted of lineage markers (Fr25lin(-)) have the capacity to differentiate and contribute to regeneration of injured islets. In this study, we assess some of the characteristics of these cells compa...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368913X676899
更新日期:2015-01-01 00:00:00
abstract::Transplanted embryonic stem (ES) cells, following myocardial infarction (MI), contribute to limited cardiac repair and regeneration with improved function. Therefore, novel strategies are still needed to understand the effects of genetically modified transplanted stem cells on cardiac remodeling. The present study ev...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368911X627561
更新日期:2012-01-01 00:00:00
abstract::Hepatocyte transplantation (HT) has become an effective therapy for patients with metabolic inborn errors. We report the clinical outcome of four children with metabolic inborn errors that underwent HT, describing the cell infusion protocol and the metabolic outcome of transplanted patients. Cryopreserved hepatocytes...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368912X637505
更新日期:2012-01-01 00:00:00
abstract::Traumatic brain injury (TBI), a major cause of mortality and morbidity, affects 10 million people worldwide, with limited treatment options. We have previously shown that (-)-phenserine (Phen), an acetylcholinesterase inhibitor originally designed and tested in clinical phase III trials for Alzheimer's disease, can re...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689719854693
更新日期:2019-09-01 00:00:00
abstract::Apoptosis is a vital pathological factor that accounts for the poor prognosis of traumatic spinal cord injury (t-SCI). The 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFKFB3) is a critical regulator for energy metabolism and proven to have antiapoptotic effects. This study aimed to investigate the neuroprote...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689720950226
更新日期:2020-01-01 00:00:00
abstract::Oligodendrocyte (OL) replacement can be a promising strategy for spinal cord injury (SCI) repair. However, the poor posttransplantation survival and inhibitory properties to axonal regeneration are two major challenges that limit their use as donor cells for repair of CNS injuries. Therefore, strategies aimed at enhan...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368912X661373
更新日期:2013-01-01 00:00:00
abstract::Bone marrow-derived mesenchymal stem cells (MSCs) are multipotent cells characterized by their self-renewal and differentiation potential. Accumulating clinical and preclinical evidence indicate MSCs are a promising cell source for regenerative medical therapies. However, undesirable immortalization, spontaneous trans...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368909788534906
更新日期:2009-01-01 00:00:00
abstract::Epithelial wound repair assures the recovery of the epithelial barrier after wounding. During wound healing epithelial cells migrate to cover the wound surface. The presented experiments were carried out to compare the migration of human keratinocytes from primary and secondary culture on polystyrene, collagen, and fi...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
abstract::Major skin burns are difficult to treat. Patients often require special care and long-term hospitalization. Besides specific complications associated with the wounds themselves, there may be impairment of the immune system and of other organs. Mesenchymal stromal cells (MSCs) are a recent therapeutic alternative to tr...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368915X688128
更新日期:2016-01-01 00:00:00
abstract::Huntington's disease (HD) is a devastating neurodegenerative disorder that occurs in patients with a mutation in the huntingtin or IT15 gene. Patients are plagued by early cognitive signs, motor deficits, and psychiatric disturbances. Symptoms are attributed to cell death in the striatum and disruption of cortical-str...
journal_title:Cell transplantation
pub_type: 杂志文章,评审
doi:10.3727/000000007783464687
更新日期:2007-01-01 00:00:00