Abstract:
:Major skin burns are difficult to treat. Patients often require special care and long-term hospitalization. Besides specific complications associated with the wounds themselves, there may be impairment of the immune system and of other organs. Mesenchymal stromal cells (MSCs) are a recent therapeutic alternative to treat burns, mainly aiming to accelerate the healing process. Several MSC properties favor their use as therapeutic approach, as they promote angiogenesis, stimulate regeneration, and enhance the immunoregulatory function. Moreover, since patients with extensive burns require urgent treatment and because the expansion of autologous MSCs is a time-consuming process, in this present study we chose to evaluate the therapeutic potential of xenogeneic MSCs in the treatment of severe burns in rats. MSCs were isolated from mouse bone marrow, expanded in vitro, and intradermally injected in the periphery of burn wounds. MSC-treated rats presented higher survival rates (76.19%) than control animals treated with PBS (60.86%, p < 0.05). In addition, 60 days after the thermal injury, the MSC-treated group showed larger proportion of healed areas within the burn wounds (90.81 ± 5.05%) than the PBS-treated group (76.11 ± 3.46%, p = 0.03). We also observed that CD4(+) and CD8(+) T cells in spleens and in damaged skin, as well as the percentage of neutrophils in the burned area, were modulated by MSC treatment. Plasma cytokine (TGF-β, IL-10, IL-6, and CINC-1) levels were also altered in the MSC-treated rats, when compared to controls. Number of injected GFP(+) MSCs progressively decreased over time, and 60 days after injection, few MSCs were still detected in the skin of treated animals. This study demonstrates the therapeutic effectiveness of intradermal application of MSCs in a rat model of deep burns, providing basis for future regenerative therapies in patients suffering from deep burn injuries.
journal_name
Cell Transplantjournal_title
Cell transplantationauthors
Caliari-Oliveira C,Yaochite JN,Ramalho LN,Palma PV,Carlos D,Cunha Fde Q,De Souza DA,Frade MA,Covas DT,Malmegrim KC,Oliveira MC,Voltarelli JCdoi
10.3727/096368915X688128subject
Has Abstractpub_date
2016-01-01 00:00:00pages
201-15issue
2eissn
0963-6897issn
1555-3892pii
content-CT-1389_Caliari-Oliveira_et_aljournal_volume
25pub_type
杂志文章abstract::There is conflicting evidence favoring both the use of human serum (HS) and of human serum albumin (HSA) in human islet culture. We evaluated the effects of HS versus HSA supplementation on 1) in vitro β-cell viability and function and 2) in vivo islet graft revascularization, islet viability, β-cell death, and metabo...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368915X688119
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abstract::The formation of a new microvasculature is essential for the long-term survival and function of the islet graft. In this study we examined endothelium of isolated pancreatic islets by stimulation with growth factors, different culture conditions, and genetic modification. We also inspected the effect of immunosuppress...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000006783981774
更新日期:2006-01-01 00:00:00
abstract::Hepatocyte transplantation and artificial organ hepatic support require a number of functionally mature hepatocytes. However, their growth activity and functional behaviors are much smaller in culture after isolation from the liver. We examined whether continuously differentiating hepatocytes from multipotent hepatic ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000003108747028
更新日期:2003-01-01 00:00:00
abstract::Exenatide is an analog of the incretin hormone glucagon-like peptide (GLP-1) that is used for the treatment of T2D for their metabolic effects. In addition to its insulinotropic effects, exenatide increases functional islet mass and improves their survival. Improved outcomes have been reported in recent clinical isle...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368911X576027
更新日期:2012-01-01 00:00:00
abstract::Magnetic targeting has the potential to enhance the therapeutic effects of stem cells through increasing retention of transplanted cells. To investigate the effects of magnetic targeting intensities on cell transplantation, we performed different magnetic intensities for mesenchymal stem cell (MSC)-targeting therapy i...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368914X685302
更新日期:2015-01-01 00:00:00
abstract::International standards for stem cell treatment of neurological disorders have not yet been established. In particular, specific quantitative methods have not yet been adopted to assess the effectiveness of stem cell treatment. The aim of this study is to evaluate the functional changes detectable by conventional neu...
journal_title:Cell transplantation
pub_type: 临床试验,杂志文章
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更新日期:2012-01-01 00:00:00
abstract::Sciatic nerve injuries, not uncommon in trauma with a limited degree of functional recovery, are considered a persistent clinical, social, and economic problem worldwide. Accumulating evidence suggests that stem cells can promote the tissue regeneration through various mechanisms. The aim of the present study was to i...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689720919438
更新日期:2020-01-01 00:00:00
abstract::During in vivo intracerebral infusions, the ability to perform accurate targeting towards a 3D-specific point allows control of the anatomical variable and identification of the effects of variations in other factors. Intraoperative MRI navigation systems are currently being used in the clinic, yet their use in nonhum...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368910X514323
更新日期:2010-01-01 00:00:00
abstract::A population of Hoechst 33342-stained cells, termed side population (SP) cells, can reconstitute the hematopoietic system of syngeneic mice. This study examined whether limiting numbers of SP cells can repopulate mice across a xenogeneic MHC class I barrier. SP cells were isolated from HLA.B7 and HLA.A2.1 transgenic m...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
abstract::The integrity of the blood-brain barrier (BBB) plays a vital role in affecting the prognosis of subarachnoid hemorrhage (SAH). This study aimed to investigate activation of the Tropomyosin-related kinase receptor B (TrkB) and its downstream signaling pathway on preserving BBB breakdown after experimental SAH. An endov...
journal_title:Cell transplantation
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doi:10.1177/0963689719857649
更新日期:2019-08-01 00:00:00
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journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689720950226
更新日期:2020-01-01 00:00:00
abstract::The incidence of cell transplant-transmitted infection is unknown and can only be inferred from prospective studies--that have not yet been performed and reported. The possibility of donor-to-recipient disease transmission through cell transplant therapy can be considered by reviewing the risk associated with other tr...
journal_title:Cell transplantation
pub_type: 杂志文章,评审
doi:10.1016/0963-6897(95)00035-v
更新日期:1995-09-01 00:00:00
abstract::The present study aimed to elucidate the mechanisms by which hydrogen sulfide (H2S) attenuates left ventricular remodeling after myocardial infarction (MI). MI was created in mice by left coronary artery ligation. One group of mice received injections of the H2S donor sodium hydrosulfide (NaHS) immediately before and ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368917X695029
更新日期:2017-05-09 00:00:00
abstract::Insufficient oxygenation can limit the long-term survival of encapsulated islets in subcutaneous tissue. Transplantation of coencapsulated pig islets with adipose or bone marrow mesenchymal stem cells (AMSCs or BM-MSCs, respectively) was investigated with regard to implant vascularization, oxygenation, and diabetes co...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368913X663550
更新日期:2014-01-01 00:00:00
abstract::Stem cells are unique cells in the ability that can self-renew and differentiate into a wide variety of cell types, suggesting that a specific molecular control network underlies these features. To date, stem cells have been applied to many clinical therapeutic approaches. For example, hematopoietic stem cells (HSCs) ...
journal_title:Cell transplantation
pub_type: 杂志文章,评审
doi:10.3727/096368909X471224
更新日期:2009-01-01 00:00:00
abstract::Cellular transplantation strategies utilizing intraspinal or intrathecal olfactory ensheathing cells (OECs) have been reported as beneficial for spinal cord injury (SCI). However, there are many disadvantages of these methods, including additional trauma to the spinal cord parenchyma and technical challenges. Therefor...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689719883842
更新日期:2019-12-01 00:00:00
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journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000003108746858
更新日期:2003-01-01 00:00:00
abstract::Achieving good islet isolation is one of the most important factors for successful islet transplantation. Porcine pancreas is suitable for islet isolation research due to its anatomical and physiological similarities to human pancreas. In this study, we evaluated a new porcine islet isolation method designed to maximi...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000005783982512
更新日期:2005-01-01 00:00:00
abstract::Although emerging evidence links mesenchymal stem cells (MSCs) with cancer metastasis, the underlying mechanisms are poorly understood. In the present study, we found that human umbilical cord-derived MSCs (UC-MSCs) promoted MCF-7 cell migration in vitro and metastasis in vivo. To explore the mechanisms, the character...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368915X687462
更新日期:2015-01-01 00:00:00
abstract::Cell escape occurs after intramyocardial injection for treatment of myocardial infarction (MI) and then the migrated cells might be entrapped by extracardiac organs. We investigated the fate of migrated bone marrow-derived mesenchymal stromal cells (MSCs) and their impact on lung, liver, and spleen. MI model was creat...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368910X513982
更新日期:2010-01-01 00:00:00
abstract::ET-Kyoto solution (ET-K) is an extracellular-type organ preservation solution containing the cytoprotective disaccharide, trehalose. A previous study reported the supplement of dibutyryl cyclic adenosine monophosphate (db-cAMP) in conventional ET-K to attenuate lung ischemia-reperfusion injury. In this study, the effi...
journal_title:Cell transplantation
pub_type: 杂志文章
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更新日期:2008-01-01 00:00:00
abstract::Human islet allografts are well known to induce full and sustained remission of hyperglycemia, with complete normalization of key metabolic parameters. Nevertheless, acquiring human islets, even from cadaveric human donor pancreases, remains a significant impediment to successful transplantation therapy for diabetes. ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000006783982223
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journal_title:Cell transplantation
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journal_title:Cell transplantation
pub_type: 临床试验,杂志文章
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更新日期:2013-01-01 00:00:00
abstract::The use of regenerative medicine to treat nervous system disorders like ataxia has been proposed to either replace or support degenerating neurons. In this study, we assessed the ability of human neural progenitor cells (hNPCs) to repair and restore the function of dying neurons within the spastic Han-Wistar rat (sHW)...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689717723637
更新日期:2017-11-01 00:00:00
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journal_title:Cell transplantation
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
abstract::The aim of this study was to evaluate the qualitative change in reparative cartilage after autologous chondrocyte implantation (ACI). Ten knees of 10 patients were studied. The signal intensities of reparative and normal cartilage were evaluated by fat-suppressed three-dimensional spoiled-gradient recalled (FS 3D-SPGR...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000005783982666
更新日期:2005-01-01 00:00:00
abstract::In this study the time course of homing and the body distribution of systemically delivered bone marrow mesenchymal stem cells (BM-MSCs) after myocardial infarction (MI) were evaluated. BM-MSCs were isolated from Wistar rats, expanded in vitro, and their phenotypical characterization was performed by flow cytometer. R...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368909X479677
更新日期:2010-01-01 00:00:00
abstract::A new extracorporeal bioartificial liver using alginate-entrapped hepatocytes was developed and evaluated for its ability to correct the lack of bilirubin conjugation in the Gunn rat. Hepatocytes were harvested from Sprague-Dawley rats by the two-step collagenase perfusion method and then immobilized in Ca(++)-alginat...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368979300200603
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journal_title:Cell transplantation
pub_type: 杂志文章,评审
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