Abstract:
:Although emerging evidence links mesenchymal stem cells (MSCs) with cancer metastasis, the underlying mechanisms are poorly understood. In the present study, we found that human umbilical cord-derived MSCs (UC-MSCs) promoted MCF-7 cell migration in vitro and metastasis in vivo. To explore the mechanisms, the characteristics of MCF-7 cells cocultured with UC-MSCs were assessed. The expression and secretion of interleukin-8 (IL-8) and IL-6 were induced in MCF-7 cells cocultured with UC-MSCs. However, neutralization of IL-8 or IL-6 secreted by UC-MSCs could attenuate the enhanced expression of IL-8 and IL-6 in MCF-7 cells cocultured with UC-MSCs, which subsequently alleviated the enhanced migration. Similar to UC-MSCs, exogenous human recombinant IL-8 or IL-6 also promoted IL-8 and IL-6 expression and MCF-7 cell migration. In addition to enhanced IL-8 and IL-6 expression, MCF-7 cells cocultured with UC-MSCs displayed enhanced mammosphere-forming ability and increased percentage of CD44(+)/CD24(-) cells. However, epithelial-to-mesenchymal transition (EMT) was not observed in MCF-7 cells cocultured with UC-MSCs. Taken together, these results suggested that IL-8 and IL-6 secreted by UC-MSCs activated the autocrine IL-8 and IL-6 signaling in MCF-7 cells and induced CD44(+)/CD24(-) cells, which subsequently promoted MCF-7 cell migration in vitro and metastasis in vivo.
journal_name
Cell Transplantjournal_title
Cell transplantationauthors
Ma F,Chen D,Chen F,Chi Y,Han Z,Feng X,Li X,Han Zdoi
10.3727/096368915X687462subject
Has Abstractpub_date
2015-01-01 00:00:00pages
2585-99issue
12eissn
0963-6897issn
1555-3892pii
content-CT-1210_Ma_et_aljournal_volume
24pub_type
杂志文章abstract::Several previous studies, suggesting the potential use of embryonic xenografts in the treatment of neurological disorders, indicate that neural growth and axonal guidance factors may function across species. In this light, blocks of fetal porcine neocortex were grafted into small cortical lesion cavities made in newbo...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000003108747343
更新日期:2003-01-01 00:00:00
abstract::In order to fully characterize and determine the therapeutic potential of adult neural progenitor cells (NPCs), it is important to be able to isolate and study NPCs from animals such as rats, in which there are existing models of brain injury and disease. The focus of this study was to characterize the cultivation, di...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000007783465253
更新日期:2007-01-01 00:00:00
abstract::We examined whether hypoxic exposure prior to the event of transplantation would have a positive or negative effect upon later islet graft function. Mouse islets exposed to hypoxic culture were transplanted into syngeneic recipients. Islet graft function, β-cell physiology, as well as molecular changes were examined. ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368912X658728
更新日期:2013-01-01 00:00:00
abstract::Previous studies in rodents have indicated that function and survival of transplanted islets can be substantially improved by mesenchymal stem cells (MSC). The few human islet studies to date have confirmed these findings but have not determined whether physical contact between MSC and islets is required or whether th...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689720952332
更新日期:2020-01-01 00:00:00
abstract::Facioscapulohumeral muscular dystrophy (FSHD) is the third most frequent inherited myopathy. We previously demonstrated that mesoangioblasts can be efficiently isolated from FSHD muscles, although their differentiation ability into skeletal muscle was variably impaired. This correlates with overall disease severity a...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368910X546571
更新日期:2011-01-01 00:00:00
abstract::During the isolation of human islets of Langerhans the digest has repeated direct contact with the ambient atmosphere. In order to fulfill GMP requirements in clinical applications, the entire cell preparation must be performed in clean room facilities. We hypothesized that the use of a closed system, which avoids the...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000007783465091
更新日期:2007-01-01 00:00:00
abstract::Magnetic targeting has the potential to enhance the therapeutic effects of stem cells through increasing retention of transplanted cells. To investigate the effects of magnetic targeting intensities on cell transplantation, we performed different magnetic intensities for mesenchymal stem cell (MSC)-targeting therapy i...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368914X685302
更新日期:2015-01-01 00:00:00
abstract::Cerebral palsy (CP) is a common disability which results in permanent chronic motor disability appearing in early childhood. Recently human umbilical cord blood mesenchymal stem cell (hUCB-MSC) infusion has emerged as a promising therapeutic strategy for CP, and the treatment efficacy remains to be confirmed by clinic...
journal_title:Cell transplantation
pub_type: 杂志文章,随机对照试验
doi:10.1177/0963689717729379
更新日期:2018-02-01 00:00:00
abstract::One of the goals of islet transplantation is to transplant viable islets without host immunosuppression. The present study was designed to determine whether pretreatment of islets with mitomycin-C (MMC) followed by culture enhances islet survival in a rat-to-mouse xenogeneic combination. WS(RT1k) rat islets pretreated...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368908786092720
更新日期:2008-01-01 00:00:00
abstract::A population of Hoechst 33342-stained cells, termed side population (SP) cells, can reconstitute the hematopoietic system of syngeneic mice. This study examined whether limiting numbers of SP cells can repopulate mice across a xenogeneic MHC class I barrier. SP cells were isolated from HLA.B7 and HLA.A2.1 transgenic m...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
abstract::A fresh osteochondral allograft is one of the most effective treatments for cartilage defects of the knee. Despite the clinical success, fresh osteochondral allografts have great limitations in relation to the short storage time that cartilage tissues can be well-preserved. Fresh osteochondral grafts are generally sto...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368970901805-604
更新日期:2009-01-01 00:00:00
abstract::Articular cartilage is an avascular tissue composed of chondrocytes, a unique cell type responsible for abundant matrix synthesis and maintenance. When damaged, it never heals spontaneously under physiological circumstances. Therefore, the delivery of mesenchymal stem cells using hydrogel has been considered for cart...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368910X557191
更新日期:2011-01-01 00:00:00
abstract::Insufficient oxygenation can limit the long-term survival of encapsulated islets in subcutaneous tissue. Transplantation of coencapsulated pig islets with adipose or bone marrow mesenchymal stem cells (AMSCs or BM-MSCs, respectively) was investigated with regard to implant vascularization, oxygenation, and diabetes co...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368913X663550
更新日期:2014-01-01 00:00:00
abstract::Regenerative medicine aims to repair and regenerate damaged cells, tissues, and organs in order to restore function. Regeneration can be obtained either by cell replacement or by stimulating the body's own repair mechanisms. Importantly, a favorable environment is required before any regenerative signal can stimulate ...
journal_title:Cell transplantation
pub_type: 杂志文章,评审
doi:10.3727/096368916X693699
更新日期:2017-04-13 00:00:00
abstract::After a unilateral striatal lesion, animals have generally been seen to have a bilateral impairment in paw reaching, with the contralateral paw being more affected. However, most studies to date have not used a pretraining paradigm to assess maximal capacity for paw reaching, to compare with any lesion-induced loss. T...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000003108746812
更新日期:2003-01-01 00:00:00
abstract::Acute kidney injury (AKI) is a major clinical problem that still has no established treatment. We investigated the efficacy of cultured human peripheral blood mononuclear cells (PBMNCs) for AKI. Ischemia/reperfusion injury (IRI) was used to induce AKI in male nonobese diabetic (NOD/severe combined immunodeficiency) mi...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689717753186
更新日期:2018-03-01 00:00:00
abstract::Human skin contains keratinocytes in the epidermis. Such cells share their ectodermal origin with the central nervous system (CNS). Recent studies have demonstrated that terminally differentiated somatic cells can adopt a pluripotent state, or can directly convert its phenotype to neurons, after ectopic expression of ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689720978219
更新日期:2021-01-01 00:00:00
abstract::Adrenal medullary tissue including chromaffin cells was grafted intrathecally in cancer patients to relieve intractable pain. The central nervous system (CNS) is considered an immune privileged site. Therefore, non-HLA-matched and unencapsulated tissue was grafted in 15 patients and 1 sham control in a series of at le...
journal_title:Cell transplantation
pub_type: 临床试验,杂志文章
doi:10.1177/096368970000900111
更新日期:2000-01-01 00:00:00
abstract::A newly developed immunosuppressant, FTY720, has a unique mechanism that is quite different from those of conventional immunosuppressants, and is presumed to be mediated through decreases in the number of peripheral lymphocytes, especially helper T cells. This study was performed to ascertain whether this innovative d...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1016/s0963-6897(98)00021-9
更新日期:1998-07-01 00:00:00
abstract::Tumor recurrence is the main limitation of liver transplantation (LT) in patients with hepatocellular carcinoma (HCC) and can be promoted by immunosuppressants. However, there is no prevention or treatment for HCC recurrence after LT. Here we describe a clinical-scale method for an adoptive immunotherapy approach that...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368911X627589
更新日期:2012-01-01 00:00:00
abstract::In Alzheimer's disease cholinergic neurons degenerate, resulting in loss of hippocampal acetylcholine. The fimbria-fornix aspiration is a well-known animal model mimicking hippocampal cholinergic deficiency. The aim of the present study was to use in vivo lipid-mediated gene transfer to introduce an expression vector ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
abstract::As a featured ocular inflammatory disease, autoimmune uveitis is the major cause of blindness in the clinic. Although current immunosuppressive regimens can alleviate the progression of autoimmune uveitis, they have serious side effects. Therefore, an alternative therapeutic strategy is urgently required. The present ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689718796196
更新日期:2018-10-01 00:00:00
abstract::It is widely believed that human embryonic stem (huES) cells may represent a valid alternative to donor pancreata as a source of islets for transplantation. Much is known about the transcription factors whose sequential activation results in the generation of islets during pancreatic development. This knowledge has be...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000006783982359
更新日期:2006-01-01 00:00:00
abstract::Cell therapy has attracted considerable interest as a promising therapeutic alternative for patients with Parkinson's disease (PD). Clinical studies have shown that grafted fetal neural tissue can achieve considerable biochemical and clinical improvements in PD. However, the source of fetal tissue grafts is limited an...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368916X691682
更新日期:2016-11-01 00:00:00
abstract::We have recently documented that oral Linomide (quinoline-3-carboxamide) prevents autoimmune insulitis, islet destruction, and diabetes in NOD mice treated at an early stage (5 wk of age) of the disease. In this report, we show that treatment of female NOD mice with advanced disease (age 23-24 wk) by syngeneic islet t...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1016/s0963-6897(96)00085-1
更新日期:1996-11-01 00:00:00
abstract::Adipose tissue contains a mesenchymal stem cell (MSC) population known as adipose-derived stem cells (ASCs) capable of differentiating into different cell types. Our aim was to induce hepatic transdifferentiation of ASCs by sequential exposure to several combinations of cytokines, growth factors, and hormones. The mos...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368909X12483162197321
更新日期:2009-01-01 00:00:00
abstract::Cell therapy is a promising treatment modality to improve heart function in acute myocardial infarction. However, the mechanisms of action and the most suitable cell type have not been finally determined. We performed a study to compare the effects of mesenchymal stem cells (MSCs) harvested from different tissues on ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368911X627462
更新日期:2012-01-01 00:00:00
abstract::The hypogonadal (HPG) mouse is a mutant that lacks a functional gonadotropin-releasing hormone (GnRH) gene. In this study, female HPG mice received bilateral intrahypothalamic implants of an immortalized GnRH-secreting cell line (GT1-7). Nine mice were tested 42- 65 days after implantation to determine whether these c...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368979300200309
更新日期:1993-05-01 00:00:00
abstract::Cloned murine endothelial cells (cEC) were used as a carrier system for introducing a foreign gene into the microvascular bed of the hind limb of inbred mice. cEC were transfected with a beta-galactosidase-neo fusion construct, which enables both selection for DNA uptake in the presence of G 418 and the staining of ce...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:
更新日期:1997-01-01 00:00:00
abstract::Cardiovascular diseases are related to many risk factors, such as diabetes, high blood pressure, smoking, and obesity. Myocardial infarction (MI), a cardiovascular disease, is the most common cause of cardiomyocyte death. In MI, hypoxia induces cardiomyocyte apoptosis; in particular, diabetes combined with MI has a sy...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368915X686995
更新日期:2015-01-01 00:00:00