Abstract:
:In Alzheimer's disease cholinergic neurons degenerate, resulting in loss of hippocampal acetylcholine. The fimbria-fornix aspiration is a well-known animal model mimicking hippocampal cholinergic deficiency. The aim of the present study was to use in vivo lipid-mediated gene transfer to introduce an expression vector coding for the acetylcholine synthesizing enzyme choline acetyltransferase into the hippocampus to replace the loss of enzyme activity after unilateral fimbria-fornix aspiration. Our data show that the lipid FuGene is useful to transfer DNA in vitro into 3T3 fibroblasts, C6 glioma cells, and primary astroglia and to express the respective enzyme. Lipid-mediated gene transfer in vivo resulted in a marked but transient expression of green fluorescent protein below the injection site peaking 5 days after the injection. Unilateral fimbria-fornix aspiration led to a marked reduction in the activity of choline acetyltransferase in the hippocampus, which was completely replaced 5 days after lipid-mediated gene transfer of the choline acetyltransferase vector. In conclusion, our data provide evidence that lipid-mediated gene transfer using FuGene is a useful tool to replace loss of choline acetyltranseferase activity in the hippocampus after fimbria-fornix aspiration; however, the lack of good gene transfer efficiency and the transient nature of expression limit its use for clinical applications.
journal_name
Cell Transplantjournal_title
Cell transplantationauthors
Weis C,Kaufmann WA,Humpel Csubject
Has Abstractpub_date
2001-01-01 00:00:00pages
681-8issue
8eissn
0963-6897issn
1555-3892journal_volume
10pub_type
杂志文章abstract::Adipose tissue contains a mesenchymal stem cell (MSC) population known as adipose-derived stem cells (ASCs) capable of differentiating into different cell types. Our aim was to induce hepatic transdifferentiation of ASCs by sequential exposure to several combinations of cytokines, growth factors, and hormones. The mos...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368909X12483162197321
更新日期:2009-01-01 00:00:00
abstract::In this study, we assessed the ability of miR-26b-5p to regulate T cell factor 4 (TCF-4) expression and thereby control human adipose-derived mesenchymal stem cell (hADMSC) adipogenic differentiation. Adipogenic medium was used to induce hADMSC differentiation over a 6-d period. The ability of miR-26b-5p to interact w...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689720934418
更新日期:2020-01-01 00:00:00
abstract::"Proof-of-principle" that cell replacement therapy works for neurodegeneration has been reported, but only using donor cells collected from fetal brain tissue obtained from surgical terminations of pregnancy. Surgical terminations of pregnancy represent an increasingly limited supply of donor cells due to the tendenc...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368910X546580
更新日期:2011-01-01 00:00:00
abstract::Several types of hair loss result from the inability of hair follicles to initiate the anagen phase of the hair regeneration cycle. Modulating signaling pathways in the hair follicle niche can stimulate entry into the anagen phase. Despite much effort, stem cell-based or pharmacological therapies to activate the hair ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368916X691466
更新日期:2016-01-01 00:00:00
abstract::Experiments on spinal cord injury (SCI) have largely focused on the transplantation of stem cells into injured spinal cords for motor recovery while neglecting to investigate bladder dysfunction. The present study was performed to investigate the effect of B10 human mesenchymal stem cells (hMSCs) directly transplanted...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368914X682125
更新日期:2015-01-01 00:00:00
abstract::Cell and tissue culture techniques have improved considerably since the first attempts to maintain explants of animal tissue in vitro. The two major developments that have allowed these improvements are the ability to produce continuous cell lines, thus allowing reproducible results to be obtained, and the definition ...
journal_title:Cell transplantation
pub_type: 杂志文章,评审
doi:10.1016/0963-6897(95)02005-5
更新日期:1996-01-01 00:00:00
abstract::The revascularization of islets of Langerhans transplanted in heterotopic sites like the liver by portal vein embolization or the renal subcapsular space is a major process necessary for the viability of grafted cells. This process has been extensively studied by different techniques and the results have shown that is...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1016/s0963-6897(97)00099-7
更新日期:1997-11-01 00:00:00
abstract::This study was designed to investigate functional recovery after the transplantation of mesenchymal stem cells (MSCs) or neurally differentiated MSCs (NMSCs) derived from bone marrow in a rat model of spinal cord injury (SCI). Sprague-Dawley rats were subjected to incomplete SCI using an NYU impactor to create a free ...
journal_title:Cell transplantation
pub_type: 杂志文章,已发布勘误
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更新日期:2016-07-01 00:00:00
abstract::Currently, different techniques to expand human hepatocytes in vitro are being investigated to generate enough cells for liver-directed cell therapies. However, based on observations in fibroblasts and other cell types, telomere attrition limits the proliferative capacity of normal somatic cells. Therefore, we explore...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000003771000138
更新日期:2003-01-01 00:00:00
abstract::The technologies for adipose tissue harvesting, processing, and transplantation have substantially evolved in the past two decades. Clinically driven advancements have paralleled a significant improvement in the understanding of cellular, molecular, and immunobiological events surrounding cell and tissue transplantati...
journal_title:Cell transplantation
pub_type: 杂志文章,评审
doi:10.3727/096368910X507187
更新日期:2010-01-01 00:00:00
abstract::Islet transplantation as a therapy or cure for type 1 diabetes has significant promise but has been limited by islet mass requirements and long-term graft failure. The intrahepatic and intravascular site may be responsible for significant loss of transplanted islets. Nonencapsulating biomaterial scaffolds provide a st...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368912X636966
更新日期:2013-01-01 00:00:00
abstract::Xenotransplantation of porcine fetal ventral mesencephalic (pfVM) cells to overcome the dopamine shortage in the striatum of patients with Parkinson's disease seems a viable alternative to allotransplantion of human fetal donor tissue, especially because the latter is complicated by both practical and ethical issues. ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000006783981846
更新日期:2006-01-01 00:00:00
abstract::Exenatide is an analog of the incretin hormone glucagon-like peptide (GLP-1) that is used for the treatment of T2D for their metabolic effects. In addition to its insulinotropic effects, exenatide increases functional islet mass and improves their survival. Improved outcomes have been reported in recent clinical isle...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368911X576027
更新日期:2012-01-01 00:00:00
abstract::A fresh osteochondral allograft is one of the most effective treatments for cartilage defects of the knee. Despite the clinical success, fresh osteochondral allografts have great limitations in relation to the short storage time that cartilage tissues can be well-preserved. Fresh osteochondral grafts are generally sto...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368970901805-604
更新日期:2009-01-01 00:00:00
abstract::Antibodies to the ligand for CD40 (CD154) have been shown to exert profound effects on the development of cell-mediated immune responses in mice. The present study shows that an antibody to human CD154 (hCD40L) inhibits in vivo Tetanus toxoid (TT) specific secondary antibody responses in hu-PBL-scid mice, as well as t...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1016/s0963-6897(97)00113-9
更新日期:1998-01-01 00:00:00
abstract::The clinical consequences of acute liver failure are associated with high mortality. Intensive medical intervention is required to treat the symptoms of liver failure, including coagulopathy, metabolic instability, and encephalopathy. Providing temporary liver support with an extracorporeal liver assist device could s...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368970000900101
更新日期:2000-01-01 00:00:00
abstract::Hypoxia is an adverse condition that can jeopardize the function of a bioartificial pancreatic construct. In this study we have investigated the effects of short-term hypoxic exposure (up to 24 h) on the bioenergetic status, metabolism, and insulin secretion of perfused pancreatic constructs composed of alginate/poly-...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368970000900312
更新日期:2000-05-01 00:00:00
abstract::Direct reprogramming of an adult cell into another differentiated lineage-such as fibroblasts into neurons, cardiomyocytes, or blood cells-without passage through an undifferentiated pluripotent stage is a new area of research that has recently emerged alongside stem cell technology and induced pluripotent stem cell r...
journal_title:Cell transplantation
pub_type: 杂志文章,评审
doi:10.3727/096368912X657477
更新日期:2013-01-01 00:00:00
abstract::Maintenance of freshly isolated porcine liver cells in vitro is limited for a short period of time. Therefore, establishment of easy handling cell lines is extremely important for in vitro study for liver cells and their possible utilization for cell differentiation and growth of stem cells. Porcine liver cells were t...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368910X508979
更新日期:2010-01-01 00:00:00
abstract::An in vivo tracer technique that uses radiolabeled insulin as the tracer molecule has been developed to assess the rate of chemical transport between the cell transplantation chamber of an implantable bioartificial device and the host's circulatory system. The device considered here employs site-directed neovasculariz...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1016/0963-6897(95)90033-0
更新日期:1995-03-01 00:00:00
abstract::Pancreatic islet transplantation has the potential to be an effective treatment for type 1 diabetes mellitus. While recent improvements have improved 1-year outcomes, follow-up studies show a persistent loss of graft function/survival over 5 years. One possible cause of islet transplant failure is the immunosuppressan...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368909X471198
更新日期:2009-01-01 00:00:00
abstract::A population of Hoechst 33342-stained cells, termed side population (SP) cells, can reconstitute the hematopoietic system of syngeneic mice. This study examined whether limiting numbers of SP cells can repopulate mice across a xenogeneic MHC class I barrier. SP cells were isolated from HLA.B7 and HLA.A2.1 transgenic m...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
abstract::Hepatocyte transplantation (HT) has become an effective therapy for patients with metabolic inborn errors. We report the clinical outcome of four children with metabolic inborn errors that underwent HT, describing the cell infusion protocol and the metabolic outcome of transplanted patients. Cryopreserved hepatocytes...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368912X637505
更新日期:2012-01-01 00:00:00
abstract::Mesenchymal stem cells (MSCs) may be of value in regeneration of renal tissue after damage; however, lack of biological knowledge and variability of results in animal models limit their utilization. We studied the effects of MSCs on podocytes in vitro and in vivo utilizing adriamycin (ADR) as a model of renal toxicity...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368908787236567
更新日期:2008-01-01 00:00:00
abstract::Stem cells from dental tissues have been isolated and established for tooth regenerative applications. However, basic characterization on their biological properties still needs to be investigated before employing them for effective clinical trials. In this study, we compared the telomere length, relative telomerase ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368911X565001
更新日期:2011-01-01 00:00:00
abstract::Cloned murine endothelial cells (cEC) were used as a carrier system for introducing a foreign gene into the microvascular bed of the hind limb of inbred mice. cEC were transfected with a beta-galactosidase-neo fusion construct, which enables both selection for DNA uptake in the presence of G 418 and the staining of ce...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:
更新日期:1997-01-01 00:00:00
abstract::Porcine neonatal islet-like cell clusters (NICCs) may be an attractive source of insulin-producing tissue for xenotransplantation in type I diabetic patients. We examined the functional and immunohistochemical outcome of the islet grafts in vitro during long-term culture and in vivo after transplantation to athymic nu...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000003783985142
更新日期:2003-01-01 00:00:00
abstract::Electrostimulation represents a well-known trophic factor for different tissues. In vitro electrostimulation of non-stem and stem cells induces myogenic predifferentiation and may be a powerful tool to generate cells with the capacity to respond to local areas of injury. We evaluated the effects of in vivo electrostim...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368912X652977
更新日期:2013-01-01 00:00:00
abstract::Huntington's disease (HD) produces severe neurodegeneration in the striatum leading to disabling motor impairments, including the loss of control of skilled reaching movements. Fetal GABAergic transplants can physically replace the lost striatal cells but with only partial success in functional recovery. Here, we aime...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368912X657918
更新日期:2013-01-01 00:00:00
abstract::Hematopoietic stem cells (HSCs) migrate to injury sites and aid in tissue repair. However, clinical success is poor and is partially due to limited HSC recruitment. We hypothesized that HSC pretreatment with H2O2 would enhance their recruitment to injured gut. As HSCs are rare cells, the number of primary cells obtain...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368912X653192
更新日期:2013-01-01 00:00:00