Abstract:
:In this study, we assessed the ability of miR-26b-5p to regulate T cell factor 4 (TCF-4) expression and thereby control human adipose-derived mesenchymal stem cell (hADMSC) adipogenic differentiation. Adipogenic medium was used to induce hADMSC differentiation over a 6-d period. The ability of miR-26b-5p to interact with the TCF-4 mRNA was confirmed through both predictive bioinformatics analyses and luciferase reporter assays. Immunofluorescent staining was used to visualize the impact of miR-26b-5p inhibition or overexpression on TCF-4 and β-catenin levels in hADMSCs. Further functional analyses were conducted by transfecting these cells with siRNAs specific for TCF-4 and β-catenin. Adipogenic marker and Wnt/β-catenin pathway gene expression levels were assessed via real-time polymerase chain reaction and western blotting. β-catenin localization was assessed via immunofluorescent staining. As expected, our adipogenic media induced the adipocytic differentiation of hADMSCs. In addition, we confirmed that TCF-4 is an miR-26b-5p target gene in these cells, and that protein levels of both TCF-4 and β-catenin were reduced when these cells were transfected with miR-26b-5p mimics. Overexpression of this microRNA also enhanced hADMSC adipogenesis, whereas TCF-4 and β-catenin overexpression inhibited this process. The enhanced hADMSC adipogenic differentiation that was observed following TCF-4 or β-catenin knockdown was partially reversed when miR-26b-5p expression was inhibited. We found that miR-26b-5p serves as a direct negative regulator of TCF-4 expression within hADMSCs, leading to inactivation of the Wnt/β-catenin pathway and thereby promoting the adipogenic differentiation of these cells in vitro.
journal_name
Cell Transplantjournal_title
Cell transplantationauthors
Luo Y,Ji H,Cao Y,Ding X,Li M,Song H,Li S,WaTableng C,Wu H,Meng J,Du Hdoi
10.1177/0963689720934418subject
Has Abstractpub_date
2020-01-01 00:00:00pages
963689720934418eissn
0963-6897issn
1555-3892journal_volume
29pub_type
杂志文章abstract::The development of new cell replacement strategies using neural stem cells (NSC) may provide an alternative and unlimited cell source for clinical neural transplantation in neurodegenerative diseases such as Parkinson's and Huntington's disease. The clinical application of neural transplantation using NSC will therefo...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000006783464426
更新日期:2006-01-01 00:00:00
abstract::Stem cell transplants into damaged myocardium may have the potential to improve cardiac function. We investigated the safety of transplanting unmanipulated autologous bone marrow into infarcted myocardium of patients undergoing coronary bypass surgery and assessed its efficacy to improve cardiac function. Fourteen pat...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000004772664842
更新日期:2004-01-01 00:00:00
abstract::Clinically, many candidates for islet transplantation are already immunized, which increases their risk of graft rejection. Encapsulation of pancreatic islets using the TheraCyte™ device has been shown to protect against allograft rejection in nonimmunized recipients. However, the capacity of the TheraCyte™ device to ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368912X657486
更新日期:2013-01-01 00:00:00
abstract::Addition of rIL-6 to IL-6-dependent B9 cells starved for IL-6 for 16-20 h stimulated a vigorous proliferative response. Glucocorticoids (GCs), in a concentration-dependent manner, inhibited rIL-6-stimulated proliferation of B9 cells This inhibition was specific for the GCs, evident by the capacity of the GCs, dexameth...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:
更新日期:2001-03-01 00:00:00
abstract::Despite the high prevalence and devastating outcome, there remain a few options for treatment of ischemic stroke. Currently available treatments are limited by a short time window for treatment and marginal efficacy when used. We have tested a human umbilical cord blood-derived stem cell line that has been shown to re...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368916X691277
更新日期:2016-01-01 00:00:00
abstract::In previous work, we engineered functional cell sheets using bone marrow-derived mesenchymal stem cells (BM-MSCs) to promote islet graft survival. In the present study, we hypothesized that a cell sheet using dermal fibroblasts could be an alternative to MSCs, and then we aimed to evaluate the effects of this cell she...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368916X690854
更新日期:2016-01-01 00:00:00
abstract::Ischemic stroke remains a serious threat to human life. There are limited effective therapies for the treatment of stroke. We have previously demonstrated that angiogenesis and neurogenesis in the brain play an important role in functional recovery following ischemic stroke. Recent studies indicate that increased arte...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689718771889
更新日期:2018-04-01 00:00:00
abstract::The anatomical specificity of axon growth from fetal pig septal xenografts was studied by transplanting septal cells from E30-35 pig fetuses into cholinergic deafferented (192-IgG-saporin-infused) rats or into aged rats (> 18 months). Cell suspensions (100,000 cells/microl) were injected bilaterally into the dorsal an...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368979900800104
更新日期:1999-01-01 00:00:00
abstract::In a companion article, we describe the engineering and characterization of pituitary GH3 cell clones stably transfected with a furin-cleavable human insulin cDNA (InsGH3 cells). This article describes the performance of InsGH3 (clones 1 and 7) cell grafts into streptozotocin (STZ)-induced diabetic nude mice. Subcutan...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368970000900610
更新日期:2000-11-01 00:00:00
abstract::Head and neck squamous cell carcinoma (HNSCC) is a malignant tumor of the upper aerodigestive tract affecting the oral cavity, lips, paranasal sinuses, larynx, and nasopharynx. Proteogenomics combines proteomics and genomics and employs mass spectrometry and high-throughput sequencing technologies to identify novel pe...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689720929308
更新日期:2020-01-01 00:00:00
abstract::Spinal cord injury (SCI) causes loss of neural functions below the level of the lesion due to interruption of spinal pathways and secondary neurodegenerative processes. The transplant of neural stem cells (NSCs) is a promising approach for the repair of SCI. Reprogramming of adult somatic cells into induced pluripoten...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368916X691312
更新日期:2016-10-01 00:00:00
abstract::We attempted to regenerate bone in a significant osseous defect with various stem cells from deciduous teeth, extracted from puppies, and grafted them into a parent canine mandible as an allograft, parent dental pulp, and bone marrow by tissue engineering and regenerative medicine technology using platelet-rich plasm...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368910X539128
更新日期:2011-01-01 00:00:00
abstract::Ischemia-reperfusion and immunosuppressive therapy are a major cause of progressive renal failure after kidney transplantation. Recent studies have shown that administration of bone marrow mesenchymal stem cells (MSCs) improves kidney functional recovery in the acute phase of post ischemia-reperfusion injury. In the ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368912X640448
更新日期:2012-01-01 00:00:00
abstract::Potential labels for identifying embryonic raphe neurons and a clonal, neuronally differentiating, raphe-derived cell line, RN33B, in CNS transplantation studies were tested by first characterizing the labels in vitro. The labels that were tested included 4',6-diamidino-2-phenylindole hydrochloride, 1,1'-dioctadecyl-3...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368979300200207
更新日期:1993-03-01 00:00:00
abstract::Traumatic brain injury (TBI), a major cause of mortality and morbidity, affects 10 million people worldwide, with limited treatment options. We have previously shown that (-)-phenserine (Phen), an acetylcholinesterase inhibitor originally designed and tested in clinical phase III trials for Alzheimer's disease, can re...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689719854693
更新日期:2019-09-01 00:00:00
abstract::Cloned murine endothelial cells (cEC) were used as a carrier system for introducing a foreign gene into the microvascular bed of the hind limb of inbred mice. cEC were transfected with a beta-galactosidase-neo fusion construct, which enables both selection for DNA uptake in the presence of G 418 and the staining of ce...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:
更新日期:1997-01-01 00:00:00
abstract::In diabetic patients cardiovascular morbidity and mortality is still a major problem. Our aim was to study the effect of kidney-pancreas transplantation on survival, cardiovascular events, and causes of death in diabetic type 1 uremic patients. Three hundred and thirty-three uremic IDDM patients were enrolled in our w...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368970000900621
更新日期:2000-11-01 00:00:00
abstract::We previously demonstrated that mesenchymal cells from human amniotic membrane (hAMTCs) inhibit the generation and maturation of monocyte-derived dendritic cells (DCs) in vitro. Considering the crucial role of DCs in the immune response and that epithelial cells of the human amniotic membrane (hAECs) share some of the...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368914X684033
更新日期:2015-01-01 00:00:00
abstract::Human islets are subjected to a number of stresses before and during their isolation that may influence their survival and engraftment after transplantation. Apoptosis is likely to be activated in response to these stresses. Apoptosis due to intrinsic stresses is regulated by pro- and antiapoptotic members of the Bcl...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368911X566262
更新日期:2012-01-01 00:00:00
abstract::Gammopathies, multiple myeloma, and amyloidosis are plasma dyscrasias characterized by clonal proliferation and immunoglobulin overproduction. Renal impairment is the most common and serious complication with an incidence of 20-30% patients at the diagnosis. Kidney transplant has not been considered feasible in the pr...
journal_title:Cell transplantation
pub_type: 杂志文章,评审
doi:10.3727/096368915X688560
更新日期:2016-01-01 00:00:00
abstract::Cell and tissue culture techniques have improved considerably since the first attempts to maintain explants of animal tissue in vitro. The two major developments that have allowed these improvements are the ability to produce continuous cell lines, thus allowing reproducible results to be obtained, and the definition ...
journal_title:Cell transplantation
pub_type: 杂志文章,评审
doi:10.1016/0963-6897(95)02005-5
更新日期:1996-01-01 00:00:00
abstract::A new extracorporeal bioartificial liver using alginate-entrapped hepatocytes was developed and evaluated for its ability to correct the lack of bilirubin conjugation in the Gunn rat. Hepatocytes were harvested from Sprague-Dawley rats by the two-step collagenase perfusion method and then immobilized in Ca(++)-alginat...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368979300200603
更新日期:1993-11-01 00:00:00
abstract::Liver transplantation is the only existing modality for treating decompensated liver cirrhosis. Several factors, such as nonavailability of donors, combined with operative risks, complications associated with rejection, usage of immunosuppressive agents, and cost intensiveness, make this strategy available to only a f...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368910X498241
更新日期:2010-01-01 00:00:00
abstract::Pancreatic beta cell lines may offer an abundant source of cells for beta-cell replacement in type I diabetes. Using regulatory elements of the bacterial tetracycline (tet) operon for conditional expression of SV40 T antigen oncoprotein in transgenic mouse beta cells, we have shown that reversible immortalization is a...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
abstract::Cell-based therapy for the bladder has its beginnings in the 1990s with the successful isolation and culture of bladder smooth muscle cells. Since then, several attempts have been made to artificially implant native cell types and stem cell-derived cells into damaged bladders in the form of single-cell injectables or ...
journal_title:Cell transplantation
pub_type: 杂志文章,评审
doi:10.3727/096368914X685050
更新日期:2015-01-01 00:00:00
abstract::Efficient maintenance of the undifferentiated status of embryonic stem cells (ESCs) may be important for preparation of high-quality cell sources that can be successfully used for stem cell research and therapy. Here we tried to identify a compound that can enhance the quality of pluripotent stem cells. Treatment of E...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368914X683511
更新日期:2015-01-01 00:00:00
abstract::We examined whether hypoxic exposure prior to the event of transplantation would have a positive or negative effect upon later islet graft function. Mouse islets exposed to hypoxic culture were transplanted into syngeneic recipients. Islet graft function, β-cell physiology, as well as molecular changes were examined. ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368912X658728
更新日期:2013-01-01 00:00:00
abstract::Bisphosphonates (BPs) are widely used to treat several metabolic and oncological diseases affecting the skeletal system. Despite BPs' well-known therapeutic potential, they also displayed important side effects, among which is BPs-related osteonecrosis of the jaw, by targeting osteoclast activities, osteoblast, and os...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689720948497
更新日期:2020-01-01 00:00:00
abstract::Adequate regulation of glucose levels by a microencapsulated pancreatic islet graft requires a minute-to-minute regulation of blood glucose. To design such a transplant, it is mandatory to have sufficient insight in factors influencing the kinetics of insulin secretion by encapsulated islets. The present study investi...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000003108747226
更新日期:2003-01-01 00:00:00
abstract::The third-generation NOD/LtSz-scid/IL2Rγ(null) (NOD/SCID IL2Rγ(null)) mouse represents a significantly improved xenograft model allowing high levels of human leukocyte engraftment over extended follow up. One remaining limitation of this mouse model, however, is the low level of circulating human erythrocytes. We esta...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368910X314161
更新日期:2010-01-01 00:00:00