Survival and function of islets during culture.

abstract：:The estimated frequency of MSCs in BM is about 0.001-0.01% of total nucleated cells. Most commonly, one applied therapeutic cell dose is about 1-5 million MSCs/kg body weight, necessitating a reliable, fast, and safe expansion system. The limited availability of MSCs demands for an extensive ex vivo amplification step...

journal_title：Cell transplantation

authors： Rojewski MT,Fekete N,Baila S,Nguyen K,Fürst D,Antwiler D,Dausend J,Kreja L,Ignatius A,Sensebé L,Schrezenmeier H

更新日期：2013-01-01 00:00:00

abstract：:Cloned murine endothelial cells (cEC) were used as a carrier system for introducing a foreign gene into the microvascular bed of the hind limb of inbred mice. cEC were transfected with a beta-galactosidase-neo fusion construct, which enables both selection for DNA uptake in the presence of G 418 and the staining of ce...

journal_title：Cell transplantation

authors： Schwärzler C,Webersinke G,Fässler R,Lametschwandtner A,Bauer H,Bauer HC

更新日期：1997-01-01 00:00:00

abstract：:As an alternative to drug immunosuppression, attempts at inducing donor-specific tolerance by intrathymic (IT) inoculations to transplant recipient of donor origin alloantigenic products has proven very promising. Using fiber optic thoracoscopy, a technique for the study of this phenomena was developed for the dog. We...

journal_title：Cell transplantation

authors： Schachner RD,Qian T,Strasser S,Brendel MD,Alejandro R,Mintz DH

更新日期：1994-07-01 00:00:00

abstract：:Simple and efficient freezing methods with maximal postthawing recovery form the basis of ideal cryopreservation. Taurine (2-amino ethanesulfonic acid), an end-product of sulphur amino acid metabolism, is one of the most abundant free amino acids in the body. The membrane stabilizing, free radical scavenging, and osmo...

journal_title：Cell transplantation

authors： Hardikar AA,Risbud MV,Remacle C,Reusens B,Hoet JJ,Bhonde RR

更新日期：2001-01-01 00:00:00

abstract：:Ischemia-reperfusion and immunosuppressive therapy are a major cause of progressive renal failure after kidney transplantation. Recent studies have shown that administration of bone marrow mesenchymal stem cells (MSCs) improves kidney functional recovery in the acute phase of post ischemia-reperfusion injury. In the ...

journal_title：Cell transplantation

authors： Alfarano C,Roubeix C,Chaaya R,Ceccaldi C,Calise D,Mias C,Cussac D,Bascands JL,Parini A

更新日期：2012-01-01 00:00:00

abstract：:In human neonates, immature GABAergic interneurons are markedly affected by an excitotoxic insult. While in adults the interest of cell transplantation has been demonstrated in several neurological disorders, few data are available regarding the immature brain. The low survival rate constitutes a strong limitation in ...

journal_title：Cell transplantation

authors： Roux C,Lesueur C,Aligny C,Brasse-Lagnel C,Genty D,Marret S,Laquerrière A,Bekri S,Gonzalez BJ

更新日期：2014-01-01 00:00:00

abstract：:Protocols of donor bone marrow infusion for tolerance induction are receiving increasing attention in clinical trials of organ allotransplantation. We report pulmonary embolism with bone fragments following vertebral body marrow infusion in a recipient of a liver and intestinal transplant. Even though pulmonary emboli...

journal_title：Cell transplantation

authors： Fernandez LA,Romaguera R,Viciana AL,Ruiz P,Tzakis AG,Ricordi C

更新日期：1996-07-01 00:00:00

abstract：:The ability to replace damaged myocardial tissue with new striated muscle would constitute a major advance in the treatment of diseases that irreversibly injure cardiac muscle cells. The creation of focal grafts of skeletal muscle has been reported following the intramural injection of skeletal myoblasts into both nor...

journal_title：Cell transplantation

authors： Robinson SW,Cho PW,Levitsky HI,Olson JL,Hruban RH,Acker MA,Kessler PD

更新日期：1996-01-01 00:00:00

abstract：:Facioscapulohumeral muscular dystrophy (FSHD) is the third most frequent inherited myopathy. We previously demonstrated that mesoangioblasts can be efficiently isolated from FSHD muscles, although their differentiation ability into skeletal muscle was variably impaired. This correlates with overall disease severity a...

journal_title：Cell transplantation

authors： Morosetti R,Gidaro T,Broccolini A,Gliubizzi C,Sancricca C,Tonali PA,Ricci E,Mirabella M

更新日期：2011-01-01 00:00:00

abstract：:Superparamagnetic iron oxide nanoparticles (MNPs) together with magnetic resonance imaging (MRI) are the preferred tools for monitoring the fate and biodistribution of administered cells in stem cell therapy studies. Commercial MNPs need transfection agents and long incubation times for sufficient cell labeling and fu...

journal_title：Cell transplantation

authors： Argibay B,Trekker J,Himmelreich U,Beiras A,Topete A,Taboada P,Pérez-Mato M,Iglesias-Rey R,Sobrino T,Rivas J,Campos F,Castillo J

更新日期：2016-10-01 00:00:00

abstract：:Islet transplantation as a therapy or cure for type 1 diabetes has significant promise but has been limited by islet mass requirements and long-term graft failure. The intrahepatic and intravascular site may be responsible for significant loss of transplanted islets. Nonencapsulating biomaterial scaffolds provide a st...

journal_title：Cell transplantation

authors： Gibly RF,Zhang X,Lowe WL Jr,Shea LD

更新日期：2013-01-01 00:00:00

abstract：:Current good manufacturing practice (cGMP) islet processing facilities provide an ultraclean environment for the safe production of clinical grade islets for transplantation into immunosuppressed diabetic recipients. The objective of this study was to monitor the rate of microbial contamination in islet products after...

journal_title：Cell transplantation

authors： Kin T,Rosichuk S,Shapiro AM,Lakey JR

更新日期：2007-01-01 00:00:00

abstract：:In diabetic patients cardiovascular morbidity and mortality is still a major problem. Our aim was to study the effect of kidney-pancreas transplantation on survival, cardiovascular events, and causes of death in diabetic type 1 uremic patients. Three hundred and thirty-three uremic IDDM patients were enrolled in our w...

journal_title：Cell transplantation

authors： La Rocca E,Fiorina P,Astorri E,Rossetti C,Lucignani G,Fazio F,Giudici D,Castoldi R,Bianchi G,Di Carlo V,Pozza G,Secchi A

更新日期：2000-11-01 00:00:00

abstract：:The purpose of this study was to investigate the clinical efficacy of neural stem/progenitor cell (NS/PC) transplantation to treat severe cortical visual impairment (CVI), a sequela of neonatal brain injury. Fifty-two patients with cerebral injury and CVI were randomly divided into two groups: the treatment group (n =...

journal_title：Cell transplantation

authors： Luan Z,Qu S,Du K,Liu W,Yang Y,Wang Z,Cui Y,Du Q

更新日期：2013-01-01 00:00:00

abstract：:It is widely believed that human embryonic stem (huES) cells may represent a valid alternative to donor pancreata as a source of islets for transplantation. Much is known about the transcription factors whose sequential activation results in the generation of islets during pancreatic development. This knowledge has be...

journal_title：Cell transplantation

authors： Dominguez-Bendala J,Pastori RL,Ricordi C,Inverardi L

更新日期：2006-01-01 00:00:00

abstract：:Pluripotent stem cells, including human embryonic stem cells and induced pluripotent stem cells, have generated much excitement about their prospects for use in cell transplantation therapies. This is largely attributable to their virtually unlimited growth potential, their ability to be precisely genetically altered ...

journal_title：Cell transplantation

authors： Robinson AJ,Zhao G,Rathjen J,Rathjen PD,Hutchinson RG,Eyre HJ,Hemsley KM,Hopwood JJ

更新日期：2010-01-01 00:00:00

abstract：:Airway epithelial cell injury is a key triggering event to activate allergic airway inflammation, such as asthma. We previously reported that administration of mesenchymal stem cells (MSCs) significantly alleviated allergic inflammation in a mouse model of asthma, and the mmu-miR-21/ACVR2A axis may be involved. Howeve...

journal_title：Cell transplantation

authors： Li CL,Xu ZB,Fan XL,Chen HX,Yu QN,Fang SB,Wang SY,Lin YD,Fu QL

更新日期：2018-03-01 00:00:00

abstract：:Isolation of islets from market-sized pigs is costly, with considerable islet losses from fragmentation occurring during isolation and tissue culture. Fetal and neonatal pigs yield insulin unresponsive islet-like cell clusters that become glucose-responsive after extended periods of time. Both issues impact clinical a...

journal_title：Cell transplantation

authors： Lamb M,Laugenour K,Liang O,Alexander M,Foster CE,Lakey JR

更新日期：2014-03-01 00:00:00

abstract：:Nowadays, cardiac regenerative medicine is facing many limitations because of the complexity to find the most suitable stem cell source and to understand the regenerative mechanisms involved. Mesenchymal stem cells (MSCs) have shown great regenerative potential due to their intrinsic properties and ability to restore ...

journal_title：Cell transplantation

authors： Siciliano C,Chimenti I,Ibrahim M,Napoletano C,Mangino G,Scafetta G,Zoccai GB,Rendina EA,Calogero A,Frati G,De Falco E

更新日期：2015-01-01 00:00:00

abstract：:Radiotherapy is an effective treatment for brain tumors but often results in cognitive deficits in survivors. Transplantation of embryonic or brain-derived neural stem/progenitor cells (BNSPCs) ameliorated cognitive impairment after irradiation (IR) in animal models. However, such an approach in patients requires a cl...

journal_title：Cell transplantation

authors： Osman AM,Zhou K,Zhu C,Blomgren K

更新日期：2014-01-01 00:00:00

abstract：:The choice of enzyme blend is critical for successful islet isolation. Islet yield, viability, integrity, and function are important factors that influence the outcome of islet transplantation. Liberase HI has been used as a standard enzyme for pancreas digestion and has successfully produced islets that reversed dia...

journal_title：Cell transplantation

authors： Iglesias I,Valiente L,Shiang KD,Ichii H,Kandeel F,Al-Abdullah IH

更新日期：2012-01-01 00:00:00

abstract：:Successful repair of damage in cerebral palsy (CP) needs effective clinical interventions other than simply symptomatic treatments. To elucidate the feasibility of using olfactory ensheathing cells (OECs) to treat CP in children and adolescents, we conducted a randomized controlled clinical trial (RCT) on 33 patients....

journal_title：Cell transplantation

authors： Chen L,Huang H,Xi H,Xie Z,Liu R,Jiang Z,Zhang F,Liu Y,Chen D,Wang Q,Wang H,Ren Y,Zhou C

更新日期：2010-01-01 00:00:00

abstract：:The development of new cell replacement strategies using neural stem cells (NSC) may provide an alternative and unlimited cell source for clinical neural transplantation in neurodegenerative diseases such as Parkinson's and Huntington's disease. The clinical application of neural transplantation using NSC will therefo...

journal_title：Cell transplantation

authors： McLeod M,Hong M,Sen A,Sadi D,Ulalia R,Behie LA,Mendez I

更新日期：2006-01-01 00:00:00

abstract：:Cell-based therapy for the bladder has its beginnings in the 1990s with the successful isolation and culture of bladder smooth muscle cells. Since then, several attempts have been made to artificially implant native cell types and stem cell-derived cells into damaged bladders in the form of single-cell injectables or ...

journal_title：Cell transplantation

authors： Gopinath C,Ponsaerts P,Wyndaele JJ

更新日期：2015-01-01 00:00:00

abstract：:Hepatocyte transplantation (HT) has become an effective therapy for patients with metabolic inborn errors. We report the clinical outcome of four children with metabolic inborn errors that underwent HT, describing the cell infusion protocol and the metabolic outcome of transplanted patients. Cryopreserved hepatocytes...

journal_title：Cell transplantation

authors： Ribes-Koninckx C,Ibars EP,Calzado Agrasot MÁ,Bonora-Centelles A,Miquel BP,Vila Carbó JJ,Aliaga ED,Pallardó JM,Gómez-Lechón MJ,Castell JV

更新日期：2012-01-01 00:00:00

abstract：:Bone marrow-derived mesenchymal stem cells (MSCs) are multipotent cells characterized by their self-renewal and differentiation potential. Accumulating clinical and preclinical evidence indicate MSCs are a promising cell source for regenerative medical therapies. However, undesirable immortalization, spontaneous trans...

journal_title：Cell transplantation

authors： Furlani D,Li W,Pittermann E,Klopsch C,Wang L,Knopp A,Jungebluth P,Thedinga E,Havenstein C,Westien I,Ugurlucan M,Li RK,Ma N,Steinhoff G

更新日期：2009-01-01 00:00:00

abstract：:Mesenchymal stem cells (MSCs) hold great promise for bone regeneration. However, the power of mesenchymal stem cells has not been applied to structural bone allografts in clinical practice. This study designed a new strategy to enhance the efficiency of allografts for segmental bone regeneration. Isolated MSCs were cu...

journal_title：Cell transplantation

authors： Zou XH,Cai HX,Yin Z,Chen X,Jiang YZ,Hu H,Ouyang HW

更新日期：2009-01-01 00:00:00

abstract：:The need for chronic immune suppression (IS) is one of the hurdles precluding widespread use of islet cell transplantation to restore glycemic control in patients with type 1 diabetes. We report the case of a healthy nonhuman primate (NHP) treated on and off for over 2.5 years with steroid-free IS, consisting of dacli...

journal_title：Cell transplantation

authors： Berman DM,Ruiz P Jr,Blandino-Rosano M,Bernal-Mizrachi E,Kenyon NS

更新日期：2019-03-01 00:00:00

abstract：:The transplantation of porcine islets of Langerhans to treat type 1 diabetes may provide a solution to the demand for insulin-producing cells. Porcine islets encapsulated in agarose-agarose macrobeads have been shown to function in nonimmunosuppressed xenogeneic models of both streptozotocin-induced and autoimmune typ...

journal_title：Cell transplantation

authors： Holdcraft RW,Gazda LS,Circle L,Adkins H,Harbeck SG,Meyer ED,Bautista MA,Martis PC,Laramore MA,Vinerean HV,Hall RD,Smith BH

更新日期：2014-01-01 00:00:00

abstract：:The cytology of peritoneal washing fluids for gastric cancer is the most basic method for judging peritoneal micrometastasis. However, the clinical value of this method is not clear at present. A retrospective analysis was performed on 277 patients with pathologically proven and surgically treated gastric cancer. The ...

journal_title：Cell transplantation

authors： Jiang RP,Xiong XJ,Qiu XS,Wang EH,Wu GP

更新日期：2019-11-01 00:00:00