Abstract:
:It is widely believed that human embryonic stem (huES) cells may represent a valid alternative to donor pancreata as a source of islets for transplantation. Much is known about the transcription factors whose sequential activation results in the generation of islets during pancreatic development. This knowledge has been used to articulate the theoretical possibility that such process might be recapitulated in vitro from stem cells. However, our understanding of the extracellular signals that prompt the developing pancreas to follow this sequence of molecular events is very limited. Also, the simplicity of in vitro systems makes it difficult, if not impossible, to mimic the complex signaling pattern observed in living embryos. Protein transduction (PT) technology may provide researchers with a new powerful tool to sequentially induce stem cell differentiation, entirely bypassing the need for unraveling the signaling pattern that drives the process in vivo. Here we discuss this novel application of the flourishing PT technology, which may revolutionize the way we direct stem cells along any specific lineage.
journal_name
Cell Transplantjournal_title
Cell transplantationauthors
Dominguez-Bendala J,Pastori RL,Ricordi C,Inverardi Ldoi
10.3727/000000006783982359subject
Has Abstractpub_date
2006-01-01 00:00:00pages
S85-90eissn
0963-6897issn
1555-3892journal_volume
15 Suppl 1pub_type
杂志文章abstract::For isolated pancreatic islet cell preparations, it is important to be able to reliably assess their mass and quality, and for clinical applications, it is part of the regulatory requirement. Accurate assessment, however, is difficult because islets are spheroid-like cell aggregates of different sizes (<50 to 500 μm) ...
journal_title:Cell transplantation
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pub_type: 杂志文章,meta分析,评审
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journal_title:Cell transplantation
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