Abstract：
:Isolation of islets from market-sized pigs is costly, with considerable islet losses from fragmentation occurring during isolation and tissue culture. Fetal and neonatal pigs yield insulin unresponsive islet-like cell clusters that become glucose-responsive after extended periods of time. Both issues impact clinical applicability and commercial scale-up. We have focused our efforts on a cost-effective scalable method of isolating viable insulin-responsive islets. Young Yorkshire pigs (mean age 20 days, range 4-30 days) underwent rapid pancreatectomy (<5 min) and partial digestion using low-dose collagenase, followed by in vitro culture at 37°C and 5% CO2 for up to 14 days. Islet viability was assessed using FDA/PI or Newport Green, and function was assessed using a glucose-stimulated insulin release (GSIR) assay. Islet yield was performed using enumeration of dithizone-stained aliquots. The young porcine (YP) islet yield at dissociation was 12.6 ± 2.1 × 10(3) IEQ (mean ± SEM) per organ and increased to 33.3 ± 6.4 × 10(3) IEQ after 7 days of in vitro culture. Viability was 97.3 ± 7% at dissociation and remained over 90% viable after 11 days in tissue culture (n = ns). Glucose responsiveness increased throughout maturation in culture. The stimulation index (SI) of the islets increased from 1.7 ± 2 on culture day 3 to 2.58 ± 0.5 on culture day 7. These results suggest that this method is both efficient and scalable for isolating and maturing insulin-responsive porcine islets in culture.
journal_name
Cell Transplantjournal_title
Cell transplantationauthors
Lamb M,Laugenour K,Liang O,Alexander M,Foster CE,Lakey JRdoi
10.3727/096368912X662372subject
Has Abstractpub_date
2014-03-01 00:00:00pages
263-72issue
3eissn
0963-6897issn
1555-3892pii
ct0986lambjournal_volume
23pub_type
杂志文章abstract：:In this study, we assessed the ability of miR-26b-5p to regulate T cell factor 4 (TCF-4) expression and thereby control human adipose-derived mesenchymal stem cell (hADMSC) adipogenic differentiation. Adipogenic medium was used to induce hADMSC differentiation over a 6-d period. The ability of miR-26b-5p to interact w...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1177/0963689720934418
更新日期：2020-01-01 00:00:00
abstract：:Huntington's disease (HD) produces severe neurodegeneration in the striatum leading to disabling motor impairments, including the loss of control of skilled reaching movements. Fetal GABAergic transplants can physically replace the lost striatal cells but with only partial success in functional recovery. Here, we aime...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368912X657918
更新日期：2013-01-01 00:00:00
abstract：:Human islet allografts are well known to induce full and sustained remission of hyperglycemia, with complete normalization of key metabolic parameters. Nevertheless, acquiring human islets, even from cadaveric human donor pancreases, remains a significant impediment to successful transplantation therapy for diabetes. ...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/000000006783982223
更新日期：2006-01-01 00:00:00
abstract：:Adipose tissue contains a mesenchymal stem cell (MSC) population known as adipose-derived stem cells (ASCs) capable of differentiating into different cell types. Our aim was to induce hepatic transdifferentiation of ASCs by sequential exposure to several combinations of cytokines, growth factors, and hormones. The mos...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368909X12483162197321
更新日期：2009-01-01 00:00:00
abstract：:Current good manufacturing practice (cGMP) islet processing facilities provide an ultraclean environment for the safe production of clinical grade islets for transplantation into immunosuppressed diabetic recipients. The objective of this study was to monitor the rate of microbial contamination in islet products after...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：
更新日期：2007-01-01 00:00:00
abstract：:Cerebral palsy (CP) is a common disability which results in permanent chronic motor disability appearing in early childhood. Recently human umbilical cord blood mesenchymal stem cell (hUCB-MSC) infusion has emerged as a promising therapeutic strategy for CP, and the treatment efficacy remains to be confirmed by clinic...
journal_title：Cell transplantation
pub_type： 杂志文章,随机对照试验
doi：10.1177/0963689717729379
更新日期：2018-02-01 00:00:00
abstract：:Cell therapy has attracted considerable interest as a promising therapeutic alternative for patients with Parkinson's disease (PD). Clinical studies have shown that grafted fetal neural tissue can achieve considerable biochemical and clinical improvements in PD. However, the source of fetal tissue grafts is limited an...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368916X691682
更新日期：2016-11-01 00:00:00
abstract：:International standards for stem cell treatment of neurological disorders have not yet been established. In particular, specific quantitative methods have not yet been adopted to assess the effectiveness of stem cell treatment. The aim of this study is to evaluate the functional changes detectable by conventional neu...
journal_title：Cell transplantation
pub_type： 临床试验,杂志文章
doi：10.3727/096368912x633761
更新日期：2012-01-01 00:00:00
abstract：:Expression of a fluorescent reporter gene has been studied using two alternate promoters to transcribe the green fluorescent protein (gfp) from Aequorea victoria. The human cytomegalovirus (CMV) enhancer/ promoter or the human muscle-specific creatine kinase promoter (CKM) were inserted along with the gfp cDNA into a ...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1016/0963-6897(95)02026-8
更新日期：1996-05-01 00:00:00
abstract：:The neurorestorative effects of exogenous neurturin (NTN) delivered directly into the putamen via multiport catheters were studied in 10 MPTP-lesioned rhesus monkeys expressing stable parkinsonism. The parkinsonian animals were blindly assigned to receive coded solutions containing either vehicle (n = 5) or NTN (n = 5...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：
更新日期：2008-01-01 00:00:00
abstract：:Bone nonunion is a pathological condition in which all bone healing processes have stopped, resulting in abnormal mobility between 2 bone segments. The incidence of bone-related injuries will increase in an aging population, leading to such injuries reaching epidemic proportions. Tissue engineering and cell therapy us...
journal_title：Cell transplantation
pub_type： 杂志文章,评审
doi：10.1177/0963689717721203
更新日期：2017-09-01 00:00:00
abstract：:Porcine islets show notoriously low insulin secretion levels in response to glucose stimulation. While this is somehow expected in the case of immature islets isolated from fetal and neonatal pigs, disappointingly low secretory responses are frequently reported in studies using in vitro-maturated fetal and neonatal is...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368916X693798
更新日期：2017-05-09 00:00:00
abstract：:The requirement for more effective cryopreservation (CP) methodologies in support of the emerging fields of cell bioprocessing and cell therapy is now critical. Current CP strategies appropriately focus on minimizing the damaging actions of physicochemical stressors and membrane disruption associated with extra- and i...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：:Schwann cells naturally support axonal regeneration after injury in the peripheral nervous system, and have also shown a significant, albeit limited, ability to support axonal growth and remyelination after grafting to the central nervous system (CNS). It is possible that Schwann cell-induced axonal growth in the CNS ...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1016/s0963-6897(97)00166-8
更新日期：1998-03-01 00:00:00
abstract：:We examined a possible protective effect of the nonpeptidyl low molecular weight radical scavenger IAC [bis(1-hydroxy-2,2,6,6-tetramethyl-4-piperidinyl)decanedioate di-hydrochloride] on isolated human islet cells against isolation and culture oxidative stress. Islets isolated from pancreases of nondiabetic multiorgan ...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368908787236639
更新日期：2008-01-01 00:00:00
abstract：:Glioblastoma multiforme (GBM) displays high resistance to radiation and chemotherapy, due to the presence of a fraction of GBM stem-like cells (GSLCs), which are thus representing the target for GBM elimination. Since mesenchymal stem cells (MSCs) display high tumor tropism, we examined possible antitumor effects of t...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368915X687787
更新日期：2015-01-01 00:00:00
abstract：:The immunomodulatory characteristics of mesenchymal stromal cells (MSCs) may lead to multifaceted strategies in rejection of organ transplantation. This study was designed to investigate, first, the effect of the donor-type MSCs from Wistar rats on the immune system of immunocompetent Lewis rats and, second, the rejec...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368917X695010
更新日期：2017-06-09 00:00:00
abstract：:Mesenchymal stem cells (MSCs) hold great promise for bone regeneration. However, the power of mesenchymal stem cells has not been applied to structural bone allografts in clinical practice. This study designed a new strategy to enhance the efficiency of allografts for segmental bone regeneration. Isolated MSCs were cu...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368909788809839
更新日期：2009-01-01 00:00:00
abstract：:Neuronal progenitor cells (NPCs) derived from human embryonic stem cells (hESCs) are an excellent cell source for transplantation therapy due to their availability and ethical acceptability. However, the traditional method of expansion and differentiation of hESCs into NPCs in monolayer cultures requires a long time, ...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368915X690378
更新日期：2016-01-01 00:00:00
abstract：:Ten mongrel dogs were used in this study. Diabetes was chemically induced in 7 dogs, and 3 dogs served as normal controls. For each diabetic dog, 5 million human bone marrow-derived mesenchymal stem cells/kg were differentiated to form insulin-producing cells using a trichostatin-based protocol. Cells were then loaded...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1177/0963689718759913
更新日期：2018-06-01 00:00:00
abstract：:During the isolation of human islets of Langerhans the digest has repeated direct contact with the ambient atmosphere. In order to fulfill GMP requirements in clinical applications, the entire cell preparation must be performed in clean room facilities. We hypothesized that the use of a closed system, which avoids the...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/000000007783465091
更新日期：2007-01-01 00:00:00
abstract：:A large proportion of islets are lost after transplantation partly due to a lack of functional vasculature. Islets revascularize from host tissue but the process takes up to 2 weeks and has been suggested to result in reduced vascular density in engrafted islets. We describe a method for observing and quantifying the ...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/000000007783464993
更新日期：2007-01-01 00:00:00
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journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368910X539128
更新日期：2011-01-01 00:00:00
abstract：:Testis-derived Sertoli cells have been used to create an immune "privileged" site outside of the testis to facilitate cell transplantation protocols for diabetes and neurodegenerative diseases. In addition to secreting immunoprotective factors, Sertoli cells also secrete growth and trophic factors that appear to enhan...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1016/s0963-6897(97)00022-5
更新日期：1997-03-01 00:00:00
abstract：:The aim of this work was to determine the effect of nicotine desensitization on dopamine (DA) release in the dorsal striatum and shell of the nucleus accumbens (NAc) from brain slices. In vitro fast-scan cyclic voltammetry analysis was used to evaluate dopamine release in the dorsal striatum and the NAc shell of Sprag...
journal_title：Cell transplantation
pub_type： 杂志文章
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更新日期：2019-03-01 00:00:00
abstract：:The aim of this study was to evaluate the qualitative change in reparative cartilage after autologous chondrocyte implantation (ACI). Ten knees of 10 patients were studied. The signal intensities of reparative and normal cartilage were evaluated by fat-suppressed three-dimensional spoiled-gradient recalled (FS 3D-SPGR...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/000000005783982666
更新日期：2005-01-01 00:00:00
abstract：:The formation of a new microvasculature is essential for the long-term survival and function of the islet graft. In this study we examined endothelium of isolated pancreatic islets by stimulation with growth factors, different culture conditions, and genetic modification. We also inspected the effect of immunosuppress...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/000000006783981774
更新日期：2006-01-01 00:00:00
abstract：:Since donor T-cells' allorecognition of host antigens is a prerequisite for the onset of graft-versus-host disease (GVHD), blocking their cellular signaling pathways can decrease the severity of GVHD. We hypothesized that epigallocatechin-3-gallate (EGCG), due to its strong affinity to macromolecules, would adhere to...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368911X623934
更新日期：2012-01-01 00:00:00
abstract：:Epithelial wound repair assures the recovery of the epithelial barrier after wounding. During wound healing epithelial cells migrate to cover the wound surface. The presented experiments were carried out to compare the migration of human keratinocytes from primary and secondary culture on polystyrene, collagen, and fi...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：:Ischemia-reperfusion and immunosuppressive therapy are a major cause of progressive renal failure after kidney transplantation. Recent studies have shown that administration of bone marrow mesenchymal stem cells (MSCs) improves kidney functional recovery in the acute phase of post ischemia-reperfusion injury. In the ...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368912X640448
更新日期：2012-01-01 00:00:00
