Abstract:
:Since donor T-cells' allorecognition of host antigens is a prerequisite for the onset of graft-versus-host disease (GVHD), blocking their cellular signaling pathways can decrease the severity of GVHD. We hypothesized that epigallocatechin-3-gallate (EGCG), due to its strong affinity to macromolecules, would adhere to surface molecules of donor T cells, inhibit their allorecognition, and attenuate GVHD in the recipient. We tested the hypothesis by treating donor splenocytes with EGCG in both in vitro and in vivo murine GVHD models. EGCG treatment decreased the proliferation of donor cells in MLR cultures and secretion of IL-2 and INF-γ. It also reduced the epitope detection of CD3ɛ, CD4, and CD28 but did not downregulate the protein expression of these molecules, suggesting blockage of cell surface stimulatory signals. Similarly, EGCG treatment did not decrease mRNA expression for some of these molecules but decreased mitogen-induced cell proliferation, indicating that EGCG did not interfere the transcription of these genes but affected cell proliferation pathways. Furthermore, EGCG-treated donor splenocytes, when transplanted into immunocompromized recipient mice, decreased of proliferation, and the treatment extended the recipients' survival at least during the early stage of GVHD. These results strongly suggest that EGCG attenuates GVHD by both blocking specific cell surface molecules and affecting the donor T-cell proliferation pathways.
journal_name
Cell Transplantjournal_title
Cell transplantationauthors
Kanamune J,Iwanaga Y,Kina T,Noguchi H,Matsumura K,Uemoto S,Hyon SHdoi
10.3727/096368911X623934subject
Has Abstractpub_date
2012-01-01 00:00:00pages
909-18issue
5eissn
0963-6897issn
1555-3892pii
ct0486kanamunejournal_volume
21pub_type
杂志文章abstract::Spinal cord injury (SCI) is a devastating neurological disease. The pathophysiological mechanisms of SCI have been reported to be relevant to central nervous system injury such as brain injury. In this study, gene expression of the brain after SCI was elucidated using transcriptome analysis to characterize the tempora...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689717715822
更新日期:2017-07-01 00:00:00
abstract::Techniques for medical tissue regeneration require an abundant source of human adult stem cells. There is increasing evidence that adipose stem cells contribute to restoration of tissue vascularization and organ function. The object of our study was to isolate and characterize adult adipose-derived stem cells from pat...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/09638910X519265
更新日期:2010-01-01 00:00:00
abstract::Cell transplantation is now being explored as a new therapeutic strategy to restore function in the diseased human central nervous system. Neural grafts show long-term survival and function in patients with Parkinson's disease but the symptomatic relief needs to be increased. Cell transplantation seems justified in pa...
journal_title:Cell transplantation
pub_type: 杂志文章,评审
doi:10.1016/0963-6897(95)00022-p
更新日期:1995-07-01 00:00:00
abstract::Researchers have accumulated a decade of experience with autologous cell therapy in the treatment of critical limb ischemia (CLI). We conducted a systematic review of clinical trials in the literature to determine the safety and efficacy of cell therapy in CLI. We searched the literature for clinical trials of autolog...
journal_title:Cell transplantation
pub_type: 杂志文章,meta分析,评审
doi:10.3727/096368912X636777
更新日期:2013-01-01 00:00:00
abstract::The midbrain inferior colliculus (IC), a major integrating center for auditory processing, provides a model for structural, functional, and behavioral recovery. The present study examined the role of IC in spatial sound detection, and the effects of neural transplantation in sparing of behavioral performance. Hooded r...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1016/0963-6897(95)02036-5
更新日期:1996-03-01 00:00:00
abstract::Ischemia-reperfusion and immunosuppressive therapy are a major cause of progressive renal failure after kidney transplantation. Recent studies have shown that administration of bone marrow mesenchymal stem cells (MSCs) improves kidney functional recovery in the acute phase of post ischemia-reperfusion injury. In the ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368912X640448
更新日期:2012-01-01 00:00:00
abstract::Prognosis of patients with heart failure remains poor despite improved conventional and interventional treatment regimens. The improvement of neovascularization and repair processes by administration of bone marrow-derived cells modestly improved the recovery after acute myocardial infarction. However, circulating pat...
journal_title:Cell transplantation
pub_type: 杂志文章,随机对照试验
doi:10.3727/096368912X654957
更新日期:2012-01-01 00:00:00
abstract::We transplanted adult whole bone marrow prelabeled with bromodeoxyuridine (BrdU) into the ischemic boundary zone of the adult rat brain at 1 day after 2 h of middle cerebral artery occlusion (MCAo). Approximately 3.3% of 10(6) transplanted bone marrow cells were BrdU reactive at 14 days after MCAo. BrdU-reactive cells...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
abstract::An important challenge in pancreatic islet transplantation in association with type 1 diabetes is to define automatic high-throughput assays for evaluation of human islet function. The physiological techniques presently used are amenable to small-scale experimental samples and produce descriptive results. The postgeno...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:
更新日期:2008-01-01 00:00:00
abstract::Mesenchymal stem cells (MSCs) hold great promise for bone regeneration. However, the power of mesenchymal stem cells has not been applied to structural bone allografts in clinical practice. This study designed a new strategy to enhance the efficiency of allografts for segmental bone regeneration. Isolated MSCs were cu...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368909788809839
更新日期:2009-01-01 00:00:00
abstract::Testis-derived Sertoli cells have been used to create an immune "privileged" site outside of the testis to facilitate cell transplantation protocols for diabetes and neurodegenerative diseases. In addition to secreting immunoprotective factors, Sertoli cells also secrete growth and trophic factors that appear to enhan...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1016/s0963-6897(97)00022-5
更新日期:1997-03-01 00:00:00
abstract::The purpose of this study was to investigate the clinical efficacy of neural stem/progenitor cell (NS/PC) transplantation to treat severe cortical visual impairment (CVI), a sequela of neonatal brain injury. Fifty-two patients with cerebral injury and CVI were randomly divided into two groups: the treatment group (n =...
journal_title:Cell transplantation
pub_type: 杂志文章,随机对照试验
doi:10.3727/096368913X672163
更新日期:2013-01-01 00:00:00
abstract::The effect of tensile stress on the orientation and phenotype of arterial smooth muscle cells (SMCs) cultured in three-dimensional (3D) type I collagen gels was morphologically investigated. Ring-shaped hybrid tissues were prepared by thermal gelation of a cold mixed solution of type I collagen and SMCs derived from b...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368979400300605
更新日期:1994-11-01 00:00:00
abstract::The human neuronal cell-1 (HCN-1) line has recently been established. Under favorable conditions, these cells differentiate into mature neuronal phenotypes. Here we report on further characterization of these cells. Cultured HCN-1 cells express fibronectin immunoreactivity and grow well on fibronectin substrate but do...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368979200100104
更新日期:1992-01-01 00:00:00
abstract::Membrane integrity fluorescent staining is used routinely to evaluate islet viability. Results are used as one of the determining factors in islet product release criteria, and are used to assess the efficacy of different culture conditions. Recently, it has been observed that there is variation in the viability stain...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000004783983701
更新日期:2004-01-01 00:00:00
abstract::Major skin burns are difficult to treat. Patients often require special care and long-term hospitalization. Besides specific complications associated with the wounds themselves, there may be impairment of the immune system and of other organs. Mesenchymal stromal cells (MSCs) are a recent therapeutic alternative to tr...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368915X688128
更新日期:2016-01-01 00:00:00
abstract::Traumatic brain injury (TBI), a major cause of mortality and morbidity, affects 10 million people worldwide, with limited treatment options. We have previously shown that (-)-phenserine (Phen), an acetylcholinesterase inhibitor originally designed and tested in clinical phase III trials for Alzheimer's disease, can re...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689719854693
更新日期:2019-09-01 00:00:00
abstract::In Alzheimer's disease cholinergic neurons degenerate, resulting in loss of hippocampal acetylcholine. The fimbria-fornix aspiration is a well-known animal model mimicking hippocampal cholinergic deficiency. The aim of the present study was to use in vivo lipid-mediated gene transfer to introduce an expression vector ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
abstract::Cell therapy has attracted considerable interest as a promising therapeutic alternative for patients with Parkinson's disease (PD). Clinical studies have shown that grafted fetal neural tissue can achieve considerable biochemical and clinical improvements in PD. However, the source of fetal tissue grafts is limited an...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368916X691682
更新日期:2016-11-01 00:00:00
abstract::A large proportion of islets are lost after transplantation partly due to a lack of functional vasculature. Islets revascularize from host tissue but the process takes up to 2 weeks and has been suggested to result in reduced vascular density in engrafted islets. We describe a method for observing and quantifying the ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000007783464993
更新日期:2007-01-01 00:00:00
abstract::Granulocyte colony-stimulating factor (G-CSF) mobilizes peripheral blood stem cells (PBSCs) derived from bone marrow. We hypothesized that intraspinal transplantation of PBSCs mobilized by G-CSF could promote functional recovery after spinal cord injury. Spinal cords of adult nonobese diabetes/severe immunodeficiency ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368915X688146
更新日期:2016-01-01 00:00:00
abstract::Addition of embryonic striatal tissue, usually as a combination of the lateral and medial ganglionic eminences, to intrastriatal mesencephalic grafts has previously been reported to enhance recovery of drug-induced rotational behavior in the host and to modify axonal fiber outgrowth from the grafted dopaminergic neuro...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:
更新日期:1997-05-01 00:00:00
abstract::Autism spectrum disorders (ASDs) are heterogeneous complex neurodevelopmental pathologies defined by behavioral symptoms, but which have well-characterized genetic, immunological, and physiological comorbidities. Despite extensive research efforts, there are presently no agreed upon therapeutic approaches for either t...
journal_title:Cell transplantation
pub_type: 临床试验,杂志文章
doi:10.3727/096368914X684916
更新日期:2014-01-01 00:00:00
abstract::There is conflicting evidence favoring both the use of human serum (HS) and of human serum albumin (HSA) in human islet culture. We evaluated the effects of HS versus HSA supplementation on 1) in vitro β-cell viability and function and 2) in vivo islet graft revascularization, islet viability, β-cell death, and metabo...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368915X688119
更新日期:2016-01-01 00:00:00
abstract::The mechanisms of donor hepatocyte integration into recipient liver are not fully understood. We investigated mechanisms of both the integration and interaction of transplanted hepatocytes with host liver cells as well as the repopulation of the host organ following intraportal transplantation. Mature hepatocytes were...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000005783983322
更新日期:2005-01-01 00:00:00
abstract::Several molecular therapies require the implantation of cells that secrete biotherapeutic molecules and imaging the location and microenvironment of the cellular implant to ascertain its function. We demonstrate noninvasive in vivo magnetic resonance imaging (MRI) of self-assembled microcontainers that are capable of ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000007783464803
更新日期:2007-01-01 00:00:00
abstract::During the isolation of human islets of Langerhans the digest has repeated direct contact with the ambient atmosphere. In order to fulfill GMP requirements in clinical applications, the entire cell preparation must be performed in clean room facilities. We hypothesized that the use of a closed system, which avoids the...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000007783465091
更新日期:2007-01-01 00:00:00
abstract::The hypogonadal (HPG) mouse is a mutant that lacks a functional gonadotropin-releasing hormone (GnRH) gene. In this study, female HPG mice received bilateral intrahypothalamic implants of an immortalized GnRH-secreting cell line (GT1-7). Nine mice were tested 42- 65 days after implantation to determine whether these c...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368979300200309
更新日期:1993-05-01 00:00:00
abstract::Persistent corneal wounds result from numerous eye disorders, and to date, available treatments often fail to accelerate reepithelialization, the key initial step in wound healing. To speed reepithelialization, we explored a cell-transfer transplant method utilizing polydimethylsiloxane (PDMS) contact lenses to delive...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000005783982783
更新日期:2005-01-01 00:00:00
abstract::Experiments on spinal cord injury (SCI) have largely focused on the transplantation of stem cells into injured spinal cords for motor recovery while neglecting to investigate bladder dysfunction. The present study was performed to investigate the effect of B10 human mesenchymal stem cells (hMSCs) directly transplanted...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368914X682125
更新日期:2015-01-01 00:00:00