Abstract:
:We examined a possible protective effect of the nonpeptidyl low molecular weight radical scavenger IAC [bis(1-hydroxy-2,2,6,6-tetramethyl-4-piperidinyl)decanedioate di-hydrochloride] on isolated human islet cells against isolation and culture oxidative stress. Islets isolated from pancreases of nondiabetic multiorgan donors by collagenase digestion were purified by density gradient centrifugation. After the isolation, islets were either exposed or not exposed for 7 days to 10 micromol/L IAC. We found that IAC markedly reduced oxidative stress and ameliorated islets function. These results suggest that the use of IAC could be an interesting pharmacological approach for the treatment of the islets before transplantation.
journal_name
Cell Transplantjournal_title
Cell transplantationauthors
Mancarella R,Del Guerra S,Masini M,Bugliani M,Valgimigli L,Pedulli GF,Paolini M,Canistro D,Armando A,Soleti A,Filipponi F,Mosca F,Boggi U,Del Prato S,Marchetti P,Lupi Rdoi
10.3727/096368908787236639subject
Has Abstractpub_date
2008-01-01 00:00:00pages
1271-6issue
10-11eissn
0963-6897issn
1555-3892journal_volume
17pub_type
杂志文章abstract::Cord blood is an attractive cell source in regenerative medicine and represents an alternative to bone marrow. The aim of this study was to investigate whether human umbilical cord blood mononuclear (HUCBM) cells might be valuable in hepatic regenerative medicine. HUCBM cells differentiated in vitro into hepatocytes, ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368908786516837
更新日期:2008-01-01 00:00:00
abstract::Prairie voles show strong pair bonding with their mating partners, and they demonstrate parental behavior toward their infants, indicating that the prairie vole is a unique animal model for analysis of molecular mechanisms of social behavior. Until a recent study, the signaling pathway of oxytocin was thought to be cr...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368916X690502
更新日期:2016-01-01 00:00:00
abstract::Porcine islets show notoriously low insulin secretion levels in response to glucose stimulation. While this is somehow expected in the case of immature islets isolated from fetal and neonatal pigs, disappointingly low secretory responses are frequently reported in studies using in vitro-maturated fetal and neonatal is...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368916X693798
更新日期:2017-05-09 00:00:00
abstract::To examine the effects of autologous sural nerve and adrenal medullary tissue intrastriatal cografts upon voluntary motor performance in parkinsonism, a non-human primate 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) model was employed to quantitatively assess skilled hand movements. Motor performance was studie...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1016/0963-6897(95)92155-r
更新日期:1995-01-01 00:00:00
abstract::The immunomodulatory characteristics of mesenchymal stromal cells (MSCs) may lead to multifaceted strategies in rejection of organ transplantation. This study was designed to investigate, first, the effect of the donor-type MSCs from Wistar rats on the immune system of immunocompetent Lewis rats and, second, the rejec...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368917X695010
更新日期:2017-06-09 00:00:00
abstract::Early graft loss in islet transplantation means that a large amount of donor islets is required. Endothelial cells and endothelial colony-forming cells (ECFCs) have been reported to improve instant blood-mediated inflammatory reaction (IBMIR) in vitro. In this study, we examined if ECFC-coated porcine islets would pre...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368912X661364
更新日期:2014-03-01 00:00:00
abstract::Transplanted embryonic stem (ES) cells, following myocardial infarction (MI), contribute to limited cardiac repair and regeneration with improved function. Therefore, novel strategies are still needed to understand the effects of genetically modified transplanted stem cells on cardiac remodeling. The present study ev...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368911X627561
更新日期:2012-01-01 00:00:00
abstract::The hypogonadal (HPG) mouse is a mutant that lacks a functional gonadotropin-releasing hormone (GnRH) gene. In this study, female HPG mice received bilateral intrahypothalamic implants of an immortalized GnRH-secreting cell line (GT1-7). Nine mice were tested 42- 65 days after implantation to determine whether these c...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368979300200309
更新日期:1993-05-01 00:00:00
abstract::Multiple sclerosis is a disease characterized by inflammation and demyelination located in the central nervous system. Experimental autoimmune encephalomyelitis (EAE) is the most common animal model for multiple sclerosis (MS). Although the roles of T cells in MS/EAE have been well investigated, little is known about ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689719852354
更新日期:2019-09-01 00:00:00
abstract::Previous studies in rodents have indicated that function and survival of transplanted islets can be substantially improved by mesenchymal stem cells (MSC). The few human islet studies to date have confirmed these findings but have not determined whether physical contact between MSC and islets is required or whether th...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689720952332
更新日期:2020-01-01 00:00:00
abstract::Bone marrow (BM) cells may interact with coronary endothelium and modulate coronary atherosclerosis. We investigated the time course of coronary luminal loss and changes in conductance after intracoronary injection of enriched hematopoietic BM stem cells in patients with previous myocardial infarction (MI). Among 24 p...
journal_title:Cell transplantation
pub_type: 杂志文章,随机对照试验
doi:10.3727/096368907783338244
更新日期:2007-01-01 00:00:00
abstract::The purpose of this study was to investigate the clinical efficacy of neural stem/progenitor cell (NS/PC) transplantation to treat severe cortical visual impairment (CVI), a sequela of neonatal brain injury. Fifty-two patients with cerebral injury and CVI were randomly divided into two groups: the treatment group (n =...
journal_title:Cell transplantation
pub_type: 杂志文章,随机对照试验
doi:10.3727/096368913X672163
更新日期:2013-01-01 00:00:00
abstract::Stem cell transplants into damaged myocardium may have the potential to improve cardiac function. We investigated the safety of transplanting unmanipulated autologous bone marrow into infarcted myocardium of patients undergoing coronary bypass surgery and assessed its efficacy to improve cardiac function. Fourteen pat...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000004772664842
更新日期:2004-01-01 00:00:00
abstract::Bone marrow-derived mesenchymal stem cells (MSCs) are multipotent cells characterized by their self-renewal and differentiation potential. Accumulating clinical and preclinical evidence indicate MSCs are a promising cell source for regenerative medical therapies. However, undesirable immortalization, spontaneous trans...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368909788534906
更新日期:2009-01-01 00:00:00
abstract::Granulocyte colony-stimulating factor (G-CSF) mobilizes peripheral blood stem cells (PBSCs) derived from bone marrow. We hypothesized that intraspinal transplantation of PBSCs mobilized by G-CSF could promote functional recovery after spinal cord injury. Spinal cords of adult nonobese diabetes/severe immunodeficiency ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368915X688146
更新日期:2016-01-01 00:00:00
abstract::Achieving good islet isolation is one of the most important factors for successful islet transplantation. Porcine pancreas is suitable for islet isolation research due to its anatomical and physiological similarities to human pancreas. In this study, we evaluated a new porcine islet isolation method designed to maximi...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000005783982512
更新日期:2005-01-01 00:00:00
abstract::There are conflicting reports on the survival and immune reaction to allografts and xenografts of cultured skin substitutes (CSS). In this study, we investigated the allogeneic and xenogeneic responses to CSS of human keratinocytes and genetically engineered CSS expressing keratinocyte growth factor (KGF) that forms a...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000004783983594
更新日期:2004-01-01 00:00:00
abstract::Neural progenitor cells (NPCs) overexpressing fibroblast growth factor 2 (FGF-2) have the distinct tendency to associate with the vasculature and establish multiple proliferative clusters in the perivascular environment after transplantation into the cerebral cortex. Strikingly, the vascular clusters of progenitor cel...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368916X690421
更新日期:2016-01-01 00:00:00
abstract::Human embryonic stem (hES) cells have the ability to differentiate into a variety of different cell lineages and potentially provide a source of differentiated cells for many therapeutic uses. Here we investigated an efficient method of hepatic differentiation from hES cells. A human ES cell line, KhES-1, was used and...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000006783981305
更新日期:2006-01-01 00:00:00
abstract::Hepatocyte transplantation is an alternative therapy to orthotopic liver transplantation for the treatment of liver diseases. However, the supply of hepatocytes is limited given the shortage of organs available to isolate good-functioning quality cells. Neonatal livers may be a potential source alternative to adult li...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368913X669743
更新日期:2014-01-01 00:00:00
abstract::Neuronal progenitor cells (NPCs) derived from human embryonic stem cells (hESCs) are an excellent cell source for transplantation therapy due to their availability and ethical acceptability. However, the traditional method of expansion and differentiation of hESCs into NPCs in monolayer cultures requires a long time, ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368915X690378
更新日期:2016-01-01 00:00:00
abstract::Several molecular therapies require the implantation of cells that secrete biotherapeutic molecules and imaging the location and microenvironment of the cellular implant to ascertain its function. We demonstrate noninvasive in vivo magnetic resonance imaging (MRI) of self-assembled microcontainers that are capable of ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000007783464803
更新日期:2007-01-01 00:00:00
abstract::The nonobese diabetic (NOD) mouse model of type 1 diabetes (T1D) was discovered by coincidence in the 1980s and has since been widely used in the investigation of T1D and diabetic complications. The current in vivo study was originally designed to prospectively assess whether hyperglycemia onset is associated with phy...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689720939127
更新日期:2020-01-01 00:00:00
abstract::Previously, we found that hepatocyte growth factor receptor (c-Met)-and alpha-fetoprotein (AFP)-expressing cells were present in adult rat bone marrow, and that these cells also expressed hematopoietic stem cell markers, such as CD34, Thy-1, and c-Kit. When bone marrow cells were cultured in a hepatocyte growth medium...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000004783983800
更新日期:2004-01-01 00:00:00
abstract::Studies of human neurodevelopmental disorders and stem cell-based regenerative transplants have been hampered by the lack of a model of the developing human brain. Stem cell-derived neurons suffer major limitations, including the ability to recapitulate the 3-dimensional architecture of a brain tissue and the represen...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689717752946
更新日期:2018-03-01 00:00:00
abstract::Adipose tissue contains a mesenchymal stem cell (MSC) population known as adipose-derived stem cells (ASCs) capable of differentiating into different cell types. Our aim was to induce hepatic transdifferentiation of ASCs by sequential exposure to several combinations of cytokines, growth factors, and hormones. The mos...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368909X12483162197321
更新日期:2009-01-01 00:00:00
abstract::Liver fibrosis is characterized by excessive accumulation of extracellular matrix components in the liver parenchyma that distorts the normal architecture and hepatic function. Progressive fibrosis could end in the advanced stage known as cirrhosis, resulting in the need to resort to liver transplantation. Amniotic me...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368916X692645
更新日期:2016-12-13 00:00:00
abstract::The integrity of the blood-brain barrier (BBB) plays a vital role in affecting the prognosis of subarachnoid hemorrhage (SAH). This study aimed to investigate activation of the Tropomyosin-related kinase receptor B (TrkB) and its downstream signaling pathway on preserving BBB breakdown after experimental SAH. An endov...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689719857649
更新日期:2019-08-01 00:00:00
abstract::An in vivo tracer technique that uses radiolabeled insulin as the tracer molecule has been developed to assess the rate of chemical transport between the cell transplantation chamber of an implantable bioartificial device and the host's circulatory system. The device considered here employs site-directed neovasculariz...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1016/0963-6897(95)90033-0
更新日期:1995-03-01 00:00:00
abstract::Despite the high prevalence and devastating outcome, there remain a few options for treatment of ischemic stroke. Currently available treatments are limited by a short time window for treatment and marginal efficacy when used. We have tested a human umbilical cord blood-derived stem cell line that has been shown to re...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368916X691277
更新日期:2016-01-01 00:00:00