Abstract：
:Previously, we found that hepatocyte growth factor receptor (c-Met)-and alpha-fetoprotein (AFP)-expressing cells were present in adult rat bone marrow, and that these cells also expressed hematopoietic stem cell markers, such as CD34, Thy-1, and c-Kit. When bone marrow cells were cultured in a hepatocyte growth medium (HGM) with HGF and EGF, colonies composed of polygonal cells resembling mature hepatocytes appeared by 2 weeks and grew very slowly because of overgrowth of stromal cells. At days 34-41, 2-mm2 sheets of hepatocyte-like cells were cut out of their colonies by scratching with an injection needle under observation with a phase contrast microscope, transferred into wells of 24-well plates, and cultured in the HGM medium in the presence or absence of HGF and EGF. When cells reached confluence, cells were detached with trypsin and EDTA and transferred step by step into bigger culture vessels. Thus, hepatocyte-like cells were expanded 1000-fold during less than 4 months. These cells were immunocytochemically stained for albumin and also for AFP and the hematopoietic stem cell markers described above, showing characteristics of oval cells. By RT-PCR, we detected mRNAs of tryptophan-2,3-dioxygenase and tyrosine aminotransferase, markers of hepatocytes at a terminal differentiation stage. The present culture system may be useful for supply of hepatocyte resources for cell transplantation therapy.
journal_name
Cell Transplantjournal_title
Cell transplantationauthors
Miyazaki M,Masaka T,Akiyama I,Nakashima E,Sakaguchi M,Huh NHdoi
10.3727/000000004783983800subject
Has Abstractpub_date
2004-01-01 00:00:00pages
385-91issue
4eissn
0963-6897issn
1555-3892journal_volume
13pub_type
杂志文章abstract：:Hematopoietic stem and progenitor cells reside within the bone marrow (BM) microenvironment. By a well-balanced interplay between self-renewal and differentiation, they ensure a lifelong supply of mature blood cells. Physiologically, multiple different cell types contribute to the regulation of stem and progenitor cel...
journal_title：Cell transplantation
pub_type： 杂志文章,评审
doi：10.1177/0963689717745890
更新日期：2018-05-01 00:00:00
abstract：:Tissue damage by ischemia/reperfusion (I/R) results from a temporary cessation of blood flow followed by the restoration of circulation. The injury depresses mitochondrial respiration, increases the production of reactive oxygen species (ROS), decreases the mitochondrial transmembrane potential, and stimulates invasio...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368912X659862
更新日期：2014-02-01 00:00:00
abstract：:Mechanical thrombectomy (MT) is effective in managing patients with acute ischemic stroke (AIS) caused by large-vessel occlusions and allows for valuable histological analysis of thrombi. However, whether bridging therapy (pretreatment with intravenous thrombolysis before MT) provides additional benefits in patients w...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1177/0963689718823206
更新日期：2019-06-01 00:00:00
abstract：:Membrane integrity fluorescent staining is used routinely to evaluate islet viability. Results are used as one of the determining factors in islet product release criteria, and are used to assess the efficacy of different culture conditions. Recently, it has been observed that there is variation in the viability stain...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/000000004783983701
更新日期：2004-01-01 00:00:00
abstract：:Pancreatic islet transplantation is limited by shortage of donor organs. Although beta-cell lines could be used, their secretion of insulin is characteristically glucose independent and immunoisolation is required. Here we show that intrasplenic transplantation of encapsulated glucose-responsive mouse insulinoma cells...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/000000005783982990
更新日期：2005-01-01 00:00:00
abstract：:Intrauterine inflammation is a significant cause of injury to the developing fetal brain. Using a preterm fetal sheep model of in utero infection, we asked whether human amnion epithelial cells (hAECs) were able to reduce inflammation-induced fetal brain injury. Surgery was undertaken on pregnant sheep at ∼105 days ge...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368916X693572
更新日期：2017-04-13 00:00:00
abstract：:The aim of this work was to determine the effect of nicotine desensitization on dopamine (DA) release in the dorsal striatum and shell of the nucleus accumbens (NAc) from brain slices. In vitro fast-scan cyclic voltammetry analysis was used to evaluate dopamine release in the dorsal striatum and the NAc shell of Sprag...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1177/0963689718775382
更新日期：2019-03-01 00:00:00
abstract：:It is difficult to normalize plasma glucose for a prolonged period of time by s.c. injection in experimental animals. The goal of this study was to determine the feasibility and the dosage of insulin needed to maintain 24-h normoglycemia in streptozotocin-diabetic Syrian hamsters with a s.c.-implanted osmotic minipump...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1177/096368979900800511
更新日期：1999-09-01 00:00:00
abstract：:Head and neck squamous cell carcinoma (HNSCC) is a malignant tumor of the upper aerodigestive tract affecting the oral cavity, lips, paranasal sinuses, larynx, and nasopharynx. Proteogenomics combines proteomics and genomics and employs mass spectrometry and high-throughput sequencing technologies to identify novel pe...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1177/0963689720929308
更新日期：2020-01-01 00:00:00
abstract：:Stem cell transplants into damaged myocardium may have the potential to improve cardiac function. We investigated the safety of transplanting unmanipulated autologous bone marrow into infarcted myocardium of patients undergoing coronary bypass surgery and assessed its efficacy to improve cardiac function. Fourteen pat...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/000000004772664842
更新日期：2004-01-01 00:00:00
abstract：:Myoblast transplantation is being considered as a potential strategy to improve muscle function in myopathies; hence, it is important to identify the transplanted cells and to have available efficient reagents to track these cells. We first validated a human to mouse xenotransplantation model warranting the complete a...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368908786991588
更新日期：2008-01-01 00:00:00
abstract：:Experiments are described using rats with two kinds of brain damage and consequent cognitive deficit (in the Morris water maze, three-door runway, and radial maze): 1) ischemic damage to the CA1 hippocampal cell field after four-vessel occlusion (4VO), and 2) damage to the forebrain cholinergic projection system by lo...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1177/096368970000900203
更新日期：2000-03-01 00:00:00
abstract：:Peripheral blood samples from HIV-seropositive individuals enrolled in a pilot clinical trial investigating the use of allogeneic dendritic cell therapy were evaluated for mixed chimerism. In this study, dendritic cells from HLA-identical, HIV-seronegative siblings were used. Patients received an infusion of dendritic...
journal_title：Cell transplantation
pub_type： 临床试验,杂志文章,随机对照试验
doi：
更新日期：2000-05-01 00:00:00
abstract：:Multiple sclerosis is a disease characterized by inflammation and demyelination located in the central nervous system. Experimental autoimmune encephalomyelitis (EAE) is the most common animal model for multiple sclerosis (MS). Although the roles of T cells in MS/EAE have been well investigated, little is known about ...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1177/0963689719852354
更新日期：2019-09-01 00:00:00
abstract：:Cellular transplantation strategies utilizing intraspinal or intrathecal olfactory ensheathing cells (OECs) have been reported as beneficial for spinal cord injury (SCI). However, there are many disadvantages of these methods, including additional trauma to the spinal cord parenchyma and technical challenges. Therefor...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1177/0963689719883842
更新日期：2019-12-01 00:00:00
abstract：:The common use of the marrow autograft mononuclear cell (MNC) count derives from positive correlative studies following allogeneic transplantation and from earlier conflicting data regarding the value of the bone marrow autograft colony-forming unit granulocyte-macrophage (CFU-GM) assay for prediction hematologic reco...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：
更新日期：1997-09-01 00:00:00
abstract：:Ensheathing glia have been demonstrated to have neuroregenerative properties but this cell type from human sources has not been extensively studied because tissue samples are not easily obtained, primary cultures are slow growing, and human cell lines are not available. We previously isolated immortalized ensheathing ...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368910X522108
更新日期：2011-01-01 00:00:00
abstract：:Studies of human neurodevelopmental disorders and stem cell-based regenerative transplants have been hampered by the lack of a model of the developing human brain. Stem cell-derived neurons suffer major limitations, including the ability to recapitulate the 3-dimensional architecture of a brain tissue and the represen...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1177/0963689717752946
更新日期：2018-03-01 00:00:00
abstract：:The development of cell- and gene-based strategies for regenerative medicine offers a therapeutic option for the repair and potential regeneration of damaged cardiac tissue post-myocardial infarction (MI). Human umbilical cord subepithelial cell-derived stem cells (hUC-SECs), human bone marrow-derived mesenchymal stem...
journal_title：Cell transplantation
pub_type： 杂志文章,评审
doi：10.3727/096368916X691501
更新日期：2016-01-01 00:00:00
abstract：:Porcine neonatal islet-like cell clusters (NICCs) may be an attractive source of insulin-producing tissue for xenotransplantation in type I diabetic patients. We examined the functional and immunohistochemical outcome of the islet grafts in vitro during long-term culture and in vivo after transplantation to athymic nu...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/000000003783985142
更新日期：2003-01-01 00:00:00
abstract：:During the past 15 years, a variety of peptides, known as protein transduction domains (PTDs), or cell-penetrating peptides (CPPs), have been characterized for their ability to translocate into live cells. There are now numerous examples of biologically active full-length proteins and peptides that have been successfu...
journal_title：Cell transplantation
pub_type： 杂志文章,评审
doi：10.3727/096368910X508744
更新日期：2010-01-01 00:00:00
abstract：:Recent advances in islet transplantation using highly purified islets and effective immunosuppression strategies have resulted in substantial improvement in achieving insulin independence in type 1 diabetes patients. However, there are side effects from long-term immunosuppression, and transplant rejection and/or the ...
journal_title：Cell transplantation
pub_type： 杂志文章,评审
doi：10.3727/000000008783907107
更新日期：2008-01-01 00:00:00
abstract：:There is still a lack of sufficient research on the mechanism behind neurogenic bladder (NB) treatment. The aim of this study was to explore the effect of overexpressed stromal cell-derived factor-1 (SDF-1) secreted by engineered immortalized mesenchymal stem cells (imMSCs) on the NB. In this study, primary bone marro...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1177/0963689720902466
更新日期：2020-01-01 00:00:00
abstract：:We examined whether hypoxic exposure prior to the event of transplantation would have a positive or negative effect upon later islet graft function. Mouse islets exposed to hypoxic culture were transplanted into syngeneic recipients. Islet graft function, β-cell physiology, as well as molecular changes were examined. ...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368912X658728
更新日期：2013-01-01 00:00:00
abstract：:Oligodendrocyte (OL) replacement can be a promising strategy for spinal cord injury (SCI) repair. However, the poor posttransplantation survival and inhibitory properties to axonal regeneration are two major challenges that limit their use as donor cells for repair of CNS injuries. Therefore, strategies aimed at enhan...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368912X661373
更新日期：2013-01-01 00:00:00
abstract：:The main purpose of this work has been to establish a new culturing technique to improve the chondrogenic commitment of isolated adult human chondrocytes, with the aim of being used during cell-based therapies or tissue engineering strategies. By using a rather novel technique to generate scaffold-free three-dimension...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368908786576499
更新日期：2008-01-01 00:00:00
abstract：:Testis-derived Sertoli cells have been used to create an immune "privileged" site outside of the testis to facilitate cell transplantation protocols for diabetes and neurodegenerative diseases. In addition to secreting immunoprotective factors, Sertoli cells also secrete growth and trophic factors that appear to enhan...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1016/s0963-6897(97)00022-5
更新日期：1997-03-01 00:00:00
abstract：:Epithelial wound repair assures the recovery of the epithelial barrier after wounding. During wound healing epithelial cells migrate to cover the wound surface. The presented experiments were carried out to compare the migration of human keratinocytes from primary and secondary culture on polystyrene, collagen, and fi...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：:Hepatocyte transplantation (HT) has become an effective therapy for patients with metabolic inborn errors. We report the clinical outcome of four children with metabolic inborn errors that underwent HT, describing the cell infusion protocol and the metabolic outcome of transplanted patients. Cryopreserved hepatocytes...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368912X637505
更新日期：2012-01-01 00:00:00
abstract：:Circular RNAs (circRNAs) could sponge micro-RNAs (miRNAs) to regulate tumor progression of hepatocellular carcinoma (HCC). Hsa_circ_104566 contributes to papillary thyroid carcinoma progression. However, the tumorigenic mechanism of hsa_circ_104566 on HCC remains enigmatic. The role of hsa_circ_104566 on HCC was there...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1177/0963689720963948
更新日期：2020-01-01 00:00:00
