Abstract：
:Porcine neonatal islet-like cell clusters (NICCs) may be an attractive source of insulin-producing tissue for xenotransplantation in type I diabetic patients. We examined the functional and immunohistochemical outcome of the islet grafts in vitro during long-term culture and in vivo after transplantation to athymic nude mice. On average we obtained 29,000 NICCs from each pancreas. In a perifusion system, NICCs responded poorly to a glucose challenge alone, but 10 mmol/L arginine elicited a fourfold increase in insulin secretion and 16.7 mmol/L glucose + 10 mmol/L arginine caused a sevenfold increase in insulin section indicating some sensitivity towards glucose. Hormone content as well as the number of hormone-containing cells increased for the first 14 days of culture. When NICCs were stained for hormones, proliferation (Ki67), and duct cells (CK7), some insulin- and glucagon-positive cells co-stained for proliferation. However no co-staining was observed between insulin- and glucagon-positive cells or between hormone-and CK-positive cells. Following transplantation of 2000 NICCs under the renal capsule of diabetic nude mice, BG levels were normalized within an average of 13 weeks. Oral and IP glucose tolerance tests revealed a normal or even faster clearance of a glucose load compared with normal controls. Immunohistochemical examination of the grafts revealed primarily insulin-positive cells. In summary, in vitro, NICCs responded to a challenge including glucose and arginine. There was a potential for expansion of the beta-cell mass of NICCs in vitro as well as in vivo where NICCs eventually may normalize blood glucose of diabetic mice.
journal_name
Cell Transplantjournal_title
Cell transplantationauthors
Nielsen TB,Yderstraede KB,Schrøder HD,Holst JJ,Brusgaard K,Beck-Nielsen Hdoi
10.3727/000000003783985142subject
Has Abstractpub_date
2003-01-01 00:00:00pages
13-25issue
1eissn
0963-6897issn
1555-3892journal_volume
12pub_type
杂志文章abstract：:Cell transplantation is now being explored as a new therapeutic strategy to restore function in the diseased human central nervous system. Neural grafts show long-term survival and function in patients with Parkinson's disease but the symptomatic relief needs to be increased. Cell transplantation seems justified in pa...
journal_title：Cell transplantation
pub_type： 杂志文章,评审
doi：10.1016/0963-6897(95)00022-p
更新日期：1995-07-01 00:00:00
abstract：:Hepatocyte-based biological therapies are increasingly envisioned for temporary support in acute liver failure and provision of specific-liver functions in liver-based metabolic deficiency. One of the hurdles to develop such therapies is severe shortage of human livers for hepatocyte isolation. To address the issue, w...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/000000002783985693
更新日期：2002-07-01 00:00:00
abstract：:Previous studies in humans with type 1 diabetes mellitus (T1D) and in nonobese diabetic mice have investigated the beneficial immunomodulatory potential of aerobic physical activity. Performing high volume of aerobic exercise may favorably regulate autoimmunity in diabetes. We tested whether increased physical activit...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368916X693022
更新日期：2017-01-24 00:00:00
abstract：:We previously demonstrated that mesenchymal cells from human amniotic membrane (hAMTCs) inhibit the generation and maturation of monocyte-derived dendritic cells (DCs) in vitro. Considering the crucial role of DCs in the immune response and that epithelial cells of the human amniotic membrane (hAECs) share some of the...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368914X684033
更新日期：2015-01-01 00:00:00
abstract：:Astrocytes protection and functional regulation are important strategies to protect against neuronal damage caused by ischemia. Activation of the delta opioid receptor (DOR) could reduce astrocytes damage, although the mechanism remains unclear. The present study aimed to test the effect of DOR activation on autophagy...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1177/0963689719825619
更新日期：2019-06-01 00:00:00
abstract：:Liver fibrosis is characterized by excessive accumulation of extracellular matrix components in the liver parenchyma that distorts the normal architecture and hepatic function. Progressive fibrosis could end in the advanced stage known as cirrhosis, resulting in the need to resort to liver transplantation. Amniotic me...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368916X692645
更新日期：2016-12-13 00:00:00
abstract：:The long-term durability of agarose microencapsulated islets against autoimmunity was evaluated in NOD mice. Islets were isolated from 6-8-week-old prediabetic male NOD mice and microencapsulated in 5% agarose hydrogel. Microencapsulated or nonencapsulated islets were transplanted into the omental pouch of spontaneous...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/000000006783981954
更新日期：2006-01-01 00:00:00
abstract：:Mononuclear stem cells have been studied for their potential in myocardial ischemia. In our previous published article, ReACT(®) phase I/II clinical trial, our results suggest that a certain cell population, promonocytes, directly correlated with the perceived angiogenesis in refractory angina patients. This study is ...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368914X681595
更新日期：2015-01-01 00:00:00
abstract：:Human skin contains keratinocytes in the epidermis. Such cells share their ectodermal origin with the central nervous system (CNS). Recent studies have demonstrated that terminally differentiated somatic cells can adopt a pluripotent state, or can directly convert its phenotype to neurons, after ectopic expression of ...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1177/0963689720978219
更新日期：2021-01-01 00:00:00
abstract：:Angiopoietin-1 (Ang1) mediates vascular maturation and immune response. Diabetes decreases Ang1 expression and disrupts Ang1/Tie2 signaling activity. Vasculotide is an Ang1 mimetic peptide, and has anti-inflammatory effects. In this study, we test the hypothesis that vasculotide treatment induces neuroprotection and d...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1177/0963689718791568
更新日期：2018-12-01 00:00:00
abstract：:Bone marrow-derived mesenchymal stem cells (MSCs) are multipotent cells characterized by their self-renewal and differentiation potential. Accumulating clinical and preclinical evidence indicate MSCs are a promising cell source for regenerative medical therapies. However, undesirable immortalization, spontaneous trans...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368909788534906
更新日期：2009-01-01 00:00:00
abstract：:The nonobese diabetic (NOD) mouse model of type 1 diabetes (T1D) was discovered by coincidence in the 1980s and has since been widely used in the investigation of T1D and diabetic complications. The current in vivo study was originally designed to prospectively assess whether hyperglycemia onset is associated with phy...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1177/0963689720939127
更新日期：2020-01-01 00:00:00
abstract：:Efficient repopulation by transplanted hepatocytes in the severely injured liver is essential for their clinical application in the treatment of acute hepatic failure. We studied here whether and how the transplanted hepatocytes are able to efficiently repopulate the toxin-induced acute injured liver. Male dipeptidyl ...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368909X12483162196647
更新日期：2009-01-01 00:00:00
abstract：:The mechanisms of donor hepatocyte integration into recipient liver are not fully understood. We investigated mechanisms of both the integration and interaction of transplanted hepatocytes with host liver cells as well as the repopulation of the host organ following intraportal transplantation. Mature hepatocytes were...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/000000005783983322
更新日期：2005-01-01 00:00:00
abstract：:Despite recent advances and promising results in children, liver cell transplantation (LCT) should still be regarded as an experimental therapy. Several substantial complications are known from animal studies and individual patients. However, safety data on liver cell infusion in children are scarce. We used LCT in fo...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368909X485058
更新日期：2010-01-01 00:00:00
abstract：:Cell escape occurs after intramyocardial injection for treatment of myocardial infarction (MI) and then the migrated cells might be entrapped by extracardiac organs. We investigated the fate of migrated bone marrow-derived mesenchymal stromal cells (MSCs) and their impact on lung, liver, and spleen. MI model was creat...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368910X513982
更新日期：2010-01-01 00:00:00
abstract：:During in vivo intracerebral infusions, the ability to perform accurate targeting towards a 3D-specific point allows control of the anatomical variable and identification of the effects of variations in other factors. Intraoperative MRI navigation systems are currently being used in the clinic, yet their use in nonhum...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368910X514323
更新日期：2010-01-01 00:00:00
abstract：:Cell therapies consist in transplanting healthy cells into a disabled tissue with the goal to repopulate it and restore its function at least partially. In muscular diseases, most of the time, myoblasts are chosen for their expansion capacity in culture. Nevertheless, cell transplantation has limitations, among them,...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368912X655028
更新日期：2012-01-01 00:00:00
abstract：:Current good manufacturing practice (cGMP) islet processing facilities provide an ultraclean environment for the safe production of clinical grade islets for transplantation into immunosuppressed diabetic recipients. The objective of this study was to monitor the rate of microbial contamination in islet products after...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：
更新日期：2007-01-01 00:00:00
abstract：:Harvesting, expansion, and directed differentiation of human bone marrow-derived mesenchymal stem cells (BM-MSCs) could provide an autologous source of surrogate β-cells that would alleviate the limitations of availability and/or allogenic rejection following pancreatic or islet transplantation. Bone marrow cells were...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368912X647162
更新日期：2013-01-01 00:00:00
abstract：:Autologous brain cell transplantation might be useful for repairing lesions and restoring function of the central nervous system. We have demonstrated that adult monkey brain cells, obtained from cortical biopsy and kept in culture for a few weeks, exhibit neural progenitor characteristics that make them useful for br...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368909X470847
更新日期：2009-01-01 00:00:00
abstract：:The main challenge of cell transplantation as a treatment of myopathies is the large amount of tissue to treat. Intravascular delivery of cells may be an ideal route if proven to be effective and safe. Given the importance of nonhuman primates for preclinical research in transplantation, we tested the intra-arterial i...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368914X685032
更新日期：2014-01-01 00:00:00
abstract：:Multiple sclerosis (MS) is a severe debilitating disorder characterized by progressive demyelination and axonal damage of the central nervous system (CNS). Current therapies for MS inhibit the immune response and demonstrate reasonable benefits if applied during the early phase of relapsing–remitting MS (RRMS) while t...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368912X657404
更新日期：2013-01-01 00:00:00
abstract：:Several clinical trials of bone marrow cell infusion in patients with liver cirrhosis (LC) have shown clinical improvement, despite conflicting results from animal models. We investigated serial pathological features and the clinical impact after autologous bone marrow infusion (ABMI) in patients with advanced LC. Ten...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368910X506863
更新日期：2010-01-01 00:00:00
abstract：:Simple and efficient freezing methods with maximal postthawing recovery form the basis of ideal cryopreservation. Taurine (2-amino ethanesulfonic acid), an end-product of sulphur amino acid metabolism, is one of the most abundant free amino acids in the body. The membrane stabilizing, free radical scavenging, and osmo...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/000000001783986756
更新日期：2001-01-01 00:00:00
abstract：:Huntington's disease (HD) is a devastating neurodegenerative disorder that occurs in patients with a mutation in the huntingtin or IT15 gene. Patients are plagued by early cognitive signs, motor deficits, and psychiatric disturbances. Symptoms are attributed to cell death in the striatum and disruption of cortical-str...
journal_title：Cell transplantation
pub_type： 杂志文章,评审
doi：10.3727/000000007783464687
更新日期：2007-01-01 00:00:00
abstract：:The integrity of the blood-brain barrier (BBB) plays a vital role in affecting the prognosis of subarachnoid hemorrhage (SAH). This study aimed to investigate activation of the Tropomyosin-related kinase receptor B (TrkB) and its downstream signaling pathway on preserving BBB breakdown after experimental SAH. An endov...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1177/0963689719857649
更新日期：2019-08-01 00:00:00
abstract：:Techniques for medical tissue regeneration require an abundant source of human adult stem cells. There is increasing evidence that adipose stem cells contribute to restoration of tissue vascularization and organ function. The object of our study was to isolate and characterize adult adipose-derived stem cells from pat...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/09638910X519265
更新日期：2010-01-01 00:00:00
abstract：:Huntington's disease (HD) produces severe neurodegeneration in the striatum leading to disabling motor impairments, including the loss of control of skilled reaching movements. Fetal GABAergic transplants can physically replace the lost striatal cells but with only partial success in functional recovery. Here, we aime...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368912X657918
更新日期：2013-01-01 00:00:00
abstract：:Peripheral blood samples from HIV-seropositive individuals enrolled in a pilot clinical trial investigating the use of allogeneic dendritic cell therapy were evaluated for mixed chimerism. In this study, dendritic cells from HLA-identical, HIV-seronegative siblings were used. Patients received an infusion of dendritic...
journal_title：Cell transplantation
pub_type： 临床试验,杂志文章,随机对照试验
doi：
更新日期：2000-05-01 00:00:00
