Abstract：
:We examined whether hypoxic exposure prior to the event of transplantation would have a positive or negative effect upon later islet graft function. Mouse islets exposed to hypoxic culture were transplanted into syngeneic recipients. Islet graft function, β-cell physiology, as well as molecular changes were examined. Expression of hypoxia-response genes in human islets pre- and posttransplant was examined by microarray. Hypoxia-preexposed murine islet grafts provided poor glycemic control in their syngeneic recipients, marked by persistent hyperglycemia and pronounced glucose intolerance with failed first- and second-phase glucose-stimulated insulin secretion in vivo. Mechanistically, hypoxic preexposure stabilized HIF-1α with a concomitant increase in hypoxic-response genes including LDHA, and a molecular gene set, which would favor glycolysis and lactate production and impair glucose sensing. Indeed, static incubation studies showed that hypoxia-exposed islets exhibited dysregulated glucose responsiveness with elevated basal insulin secretion. Isolated human islets, prior to transplantation, express a characteristic hypoxia-response gene expression signature, including high levels of LDHA, which is maintained posttransplant. Hypoxic preexposure of an islet graft drives a HIF-dependent switch to glycolysis with subsequent poor glycemic control and loss of GSIS. Early intervention to reverse or prevent these hypoxia-induced metabolic gene changes may improve clinical islet transplantation.
journal_name
Cell Transplantjournal_title
Cell transplantationauthors
Cantley J,Walters SN,Jung MH,Weinberg A,Cowley MJ,Whitworth TP,Kaplan W,Hawthorne WJ,O'Connell PJ,Weir G,Grey STdoi
10.3727/096368912X658728subject
Has Abstractpub_date
2013-01-01 00:00:00pages
2147-59issue
11eissn
0963-6897issn
1555-3892pii
ct0834cantleyjournal_volume
22pub_type
杂志文章abstract：:The incidence of cell transplant-transmitted infection is unknown and can only be inferred from prospective studies--that have not yet been performed and reported. The possibility of donor-to-recipient disease transmission through cell transplant therapy can be considered by reviewing the risk associated with other tr...
journal_title：Cell transplantation
pub_type： 杂志文章,评审
doi：10.1016/0963-6897(95)00035-v
更新日期：1995-09-01 00:00:00
abstract：:Head and neck squamous cell carcinoma (HNSCC) is a malignant tumor of the upper aerodigestive tract affecting the oral cavity, lips, paranasal sinuses, larynx, and nasopharynx. Proteogenomics combines proteomics and genomics and employs mass spectrometry and high-throughput sequencing technologies to identify novel pe...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1177/0963689720929308
更新日期：2020-01-01 00:00:00
abstract：:Stroke is one of the most devastating diseases worldwide. In recent years, a great number of studies have focused on the effects of microRNAs (miRNAs) on stroke and the results demonstrated that the expressions of miRNAs are associated with the prognosis of stroke. In the present study, we review relevant articles reg...
journal_title：Cell transplantation
pub_type： 杂志文章,评审
doi：10.1177/0963689718773361
更新日期：2018-12-01 00:00:00
abstract：:ZNF281 is one of the core transcription factors in embryonic stem cells (ESCs) and has activation and repression roles in the transcription of ESC genes. A known target molecule of Zfp281 (the mouse homologue of ZNF281) is Nanog. However, NANOG is not expressed in most human multipotent stem cells (hMSCs). Here, we in...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368912X654948
更新日期：2013-01-01 00:00:00
abstract：:In order to fully characterize and determine the therapeutic potential of adult neural progenitor cells (NPCs), it is important to be able to isolate and study NPCs from animals such as rats, in which there are existing models of brain injury and disease. The focus of this study was to characterize the cultivation, di...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/000000007783465253
更新日期：2007-01-01 00:00:00
abstract：:It is currently said that CO has anti-inflammatory and antiapoptosis effects and it has attracted attention as a medical gas. We used CO for rat hearts and conducted a preservation experiment. We isolated rat hearts, placed them into a specially made chamber, filled the chamber with a gas mixture of PCO (4,000 hPa) a...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368911X605547
更新日期：2012-01-01 00:00:00
abstract：:The transplantation of encapsulated dopamine-secreting cells into the striatum represents one potential means of treating Parkinson's disease. The present study investigated the ability of encapsulated PC12 cells, which are derived from rat pheochromocytoma, to supply L-dopa and dopamine into the primate brain in the ...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1177/096368970000900519
更新日期：2000-09-01 00:00:00
abstract：:Acute kidney injury (AKI) is a major clinical problem that still has no established treatment. We investigated the efficacy of cultured human peripheral blood mononuclear cells (PBMNCs) for AKI. Ischemia/reperfusion injury (IRI) was used to induce AKI in male nonobese diabetic (NOD/severe combined immunodeficiency) mi...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1177/0963689717753186
更新日期：2018-03-01 00:00:00
abstract：:The development of cell- and gene-based strategies for regenerative medicine offers a therapeutic option for the repair and potential regeneration of damaged cardiac tissue post-myocardial infarction (MI). Human umbilical cord subepithelial cell-derived stem cells (hUC-SECs), human bone marrow-derived mesenchymal stem...
journal_title：Cell transplantation
pub_type： 杂志文章,评审
doi：10.3727/096368916X691501
更新日期：2016-01-01 00:00:00
abstract：:The use of regenerative medicine to treat nervous system disorders like ataxia has been proposed to either replace or support degenerating neurons. In this study, we assessed the ability of human neural progenitor cells (hNPCs) to repair and restore the function of dying neurons within the spastic Han-Wistar rat (sHW)...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1177/0963689717723637
更新日期：2017-11-01 00:00:00
abstract：:A fresh osteochondral allograft is one of the most effective treatments for cartilage defects of the knee. Despite the clinical success, fresh osteochondral allografts have great limitations in relation to the short storage time that cartilage tissues can be well-preserved. Fresh osteochondral grafts are generally sto...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1177/096368970901805-604
更新日期：2009-01-01 00:00:00
abstract：:Spinal cord injury (SCI) is a devastating neurological disease. The pathophysiological mechanisms of SCI have been reported to be relevant to central nervous system injury such as brain injury. In this study, gene expression of the brain after SCI was elucidated using transcriptome analysis to characterize the tempora...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1177/0963689717715822
更新日期：2017-07-01 00:00:00
abstract：:To investigate potential cures for spinal cord injury (SCI), several researchers have transplanted neural stem/progenitor cells (NS/PCs) into the injured spinal cord by different procedures, including intralesional (IL), intrathecal (IT), and intravenous (IV) injection. However, there are no reports quantifying or com...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368910X536554
更新日期：2011-01-01 00:00:00
abstract：:Early graft loss in islet transplantation means that a large amount of donor islets is required. Endothelial cells and endothelial colony-forming cells (ECFCs) have been reported to improve instant blood-mediated inflammatory reaction (IBMIR) in vitro. In this study, we examined if ECFC-coated porcine islets would pre...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368912X661364
更新日期：2014-03-01 00:00:00
abstract：:Hepatocyte-based biological therapies are increasingly envisioned for temporary support in acute liver failure and provision of specific-liver functions in liver-based metabolic deficiency. One of the hurdles to develop such therapies is severe shortage of human livers for hepatocyte isolation. To address the issue, w...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/000000002783985693
更新日期：2002-07-01 00:00:00
abstract：:Our purpose was to evaluate whether or not and when phenotypic modulation of smooth muscle cells (SMCs) in hybrid vascular prostheses preincorporated with SMCs occurs upon implantation. Two types of hybrid vascular grafts incorporated with vascular cells derived from canine jugular veins were prepared: grafts containi...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：
更新日期：1995-11-01 00:00:00
abstract：:Xenotransplantation of porcine fetal ventral mesencephalic (pfVM) cells to overcome the dopamine shortage in the striatum of patients with Parkinson's disease seems a viable alternative to allotransplantion of human fetal donor tissue, especially because the latter is complicated by both practical and ethical issues. ...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/000000006783981846
更新日期：2006-01-01 00:00:00
abstract：:Posttransplant infusion of viable donor bone marrow cells (DBMC) has been shown in our previous studies to promote acceptance of incompatible kidney allografts in rhesus monkeys after treatment with polyclonal antithymocyte globulin to deplete peripheral T-lymphocytes. In this nonhuman primate model, the infusion of t...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1016/s0963-6897(97)00005-5
更新日期：1997-07-01 00:00:00
abstract：:Multiple sclerosis (MS) is a severe debilitating disorder characterized by progressive demyelination and axonal damage of the central nervous system (CNS). Current therapies for MS inhibit the immune response and demonstrate reasonable benefits if applied during the early phase of relapsing–remitting MS (RRMS) while t...
journal_title：Cell transplantation
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更新日期：2013-01-01 00:00:00
abstract：:Testicular germ cell tumors (TGCTs) are highly prevalent in young men aged 20-40 years and are one of the most common lethal solid tumors in men of this age. Due to the current unclear mechanism of tumor development, there is a lack of effective treatment, and therefore in-depth research of the molecular mechanism of ...
journal_title：Cell transplantation
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doi：10.1177/0963689720946653
更新日期：2020-01-01 00:00:00
abstract：:Persistent corneal wounds result from numerous eye disorders, and to date, available treatments often fail to accelerate reepithelialization, the key initial step in wound healing. To speed reepithelialization, we explored a cell-transfer transplant method utilizing polydimethylsiloxane (PDMS) contact lenses to delive...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/000000005783982783
更新日期：2005-01-01 00:00:00
abstract：:Osteoarthritis (OA) is degenerative disease, leading to pain and functional disability. It is reported that polydeoxyribonucleotide (PDRN) is a suitable therapy for OA. However, the therapeutic mechanisms of PDRN in OA are not fully understood. To investigate the effect of PDRN in an in vitro model of OA, interleukin ...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1177/0963689718804130
更新日期：2018-11-01 00:00:00
abstract：:Several types of hair loss result from the inability of hair follicles to initiate the anagen phase of the hair regeneration cycle. Modulating signaling pathways in the hair follicle niche can stimulate entry into the anagen phase. Despite much effort, stem cell-based or pharmacological therapies to activate the hair ...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368916X691466
更新日期：2016-01-01 00:00:00
abstract：:Development of a tissue-engineered neo-kidney augment (NKA) requires evaluation of defined, therapeutically relevant cell and cell/biomaterial composites (NKA constructs) for regenerative potential in mammalian kidney. Previous work identified primary renal cell populations that extended survival and improved renal f...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368911X566172
更新日期：2011-01-01 00:00:00
abstract：:Transplantation of human cells after isolation and culture has become an important alternative for treatment of acute or chronic skin wounds. To increase the efficacy and reduce cost for transplantation of skin cells, more efficient and accurate techniques for evaluation of cell proliferation are needed. Hemocytometer...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/000000006783982115
更新日期：2006-01-01 00:00:00
abstract：:Pluripotent stem cells, including human embryonic stem cells and induced pluripotent stem cells, have generated much excitement about their prospects for use in cell transplantation therapies. This is largely attributable to their virtually unlimited growth potential, their ability to be precisely genetically altered ...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368910X498944
更新日期：2010-01-01 00:00:00
abstract：REGISTRATION NUMBER OF CLINICAL TRIALS:ChiCTR1900021317. ...
journal_title：Cell transplantation
pub_type： 杂志文章
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更新日期：2020-01-01 00:00:00
abstract：:Although hepatic stem cells are believed to exist and play a critical role in developing and regenerating liver, little is known about their cell surface specificity or differentiation capabilities. To make prospective studies of hepatic stem cells possible, we established an in vitro culture system for identification...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：:International standards for stem cell treatment of neurological disorders have not yet been established. In particular, specific quantitative methods have not yet been adopted to assess the effectiveness of stem cell treatment. The aim of this study is to evaluate the functional changes detectable by conventional neu...
journal_title：Cell transplantation
pub_type： 临床试验,杂志文章
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更新日期：2012-01-01 00:00:00
abstract：:Since donor T-cells' allorecognition of host antigens is a prerequisite for the onset of graft-versus-host disease (GVHD), blocking their cellular signaling pathways can decrease the severity of GVHD. We hypothesized that epigallocatechin-3-gallate (EGCG), due to its strong affinity to macromolecules, would adhere to...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368911X623934
更新日期：2012-01-01 00:00:00
