Abstract:
:Xenotransplantation of porcine fetal ventral mesencephalic (pfVM) cells to overcome the dopamine shortage in the striatum of patients with Parkinson's disease seems a viable alternative to allotransplantion of human fetal donor tissue, especially because the latter is complicated by both practical and ethical issues. There is, however, little known about the xenospecific immune responses involved in such an intracerebral xenotransplantation. The aim of our study was to investigate whether (1) naive human peripheral blood mononuclear cells (PMBC) display cytotoxicity against pfVM cells of E28 pig fetuses, and (2) priming of human PBMC by xenogeneic antigen presenting cells (APC) modulates pfVM-directed cellular cytotoxicity. For this purpose fresh PMBC from nine individual donors were primed by incubation with either irradiated pfVM cells or porcine spleen cells (PSC) as APC in the presence of IL-2 for 1 week before assessing cytotoxicity in a 51Cr release assay. Also, direct NK reactivity and antibody-dependent cellular cytotoxicity (ADCC) of fresh PMBC against pfVM cells was assessed. No direct cytotoxicity of naive cells (either NK reactivity or ADCC) against pfVM cells could be determined. Only PMBC primed with PSC were capable of lysing pfVM cells. PBMC primed with pfVM cells did not show cytolytic capacity towards pfVM. Interestingly, large differences in xenospecific T-cell responses exist between individual donor PBMC. Thus, human T cells are capable of killing pfVM cells in a xenoreactive response, but only after priming by donor APC. The large interindividual differences between human donors in their xenoreactive response may influence patient selection for xenotransplantation and chances of graft survival for individual patients.
journal_name
Cell Transplantjournal_title
Cell transplantationauthors
Koopmans J,de Haan A,Bruin E,van der Gun I,van Dijk H,Rozing J,de Leij L,Staal Mdoi
10.3727/000000006783981846subject
Has Abstractpub_date
2006-01-01 00:00:00pages
381-7issue
5eissn
0963-6897issn
1555-3892journal_volume
15pub_type
杂志文章abstract::The revascularization of islets of Langerhans transplanted in heterotopic sites like the liver by portal vein embolization or the renal subcapsular space is a major process necessary for the viability of grafted cells. This process has been extensively studied by different techniques and the results have shown that is...
journal_title:Cell transplantation
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journal_title:Cell transplantation
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journal_title:Cell transplantation
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journal_title:Cell transplantation
pub_type: 杂志文章,评审
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journal_title:Cell transplantation
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journal_title:Cell transplantation
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pub_type: 临床试验,杂志文章
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journal_title:Cell transplantation
pub_type: 杂志文章
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更新日期:2017-10-01 00:00:00
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journal_title:Cell transplantation
pub_type: 杂志文章
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更新日期:2003-01-01 00:00:00
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journal_title:Cell transplantation
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journal_title:Cell transplantation
pub_type: 杂志文章
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更新日期:2006-01-01 00:00:00
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journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1016/0963-6897(95)02036-5
更新日期:1996-03-01 00:00:00
abstract::Pancreatic islet transplantation is limited by shortage of donor organs. Although beta-cell lines could be used, their secretion of insulin is characteristically glucose independent and immunoisolation is required. Here we show that intrasplenic transplantation of encapsulated glucose-responsive mouse insulinoma cells...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000005783982990
更新日期:2005-01-01 00:00:00
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journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000004783983378
更新日期:2004-01-01 00:00:00
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journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689720943608
更新日期:2020-01-01 00:00:00
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journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368916X693798
更新日期:2017-05-09 00:00:00
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journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689720929308
更新日期:2020-01-01 00:00:00
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journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368909X485058
更新日期:2010-01-01 00:00:00
abstract::Addition of embryonic striatal tissue, usually as a combination of the lateral and medial ganglionic eminences, to intrastriatal mesencephalic grafts has previously been reported to enhance recovery of drug-induced rotational behavior in the host and to modify axonal fiber outgrowth from the grafted dopaminergic neuro...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:
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abstract::We investigated the effect of the activation of complement in human serum on isolated adult porcine islets using an in vitro model of pig-to-human islet transplantation. Pancreata were obtained from slaughterhouse pigs (6-8 mo old). Islets were prepared by intraductal collagenase digestion followed by purification on ...
journal_title:Cell transplantation
pub_type: 杂志文章
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更新日期:1996-09-01 00:00:00
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journal_title:Cell transplantation
pub_type: 杂志文章,评审
doi:10.1016/0963-6897(95)02005-5
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journal_title:Cell transplantation
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pub_type: 杂志文章,多中心研究,随机对照试验
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更新日期:2017-02-16 00:00:00
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