Abstract:
:A fresh osteochondral allograft is one of the most effective treatments for cartilage defects of the knee. Despite the clinical success, fresh osteochondral allografts have great limitations in relation to the short storage time that cartilage tissues can be well-preserved. Fresh osteochondral grafts are generally stored in culture medium at 4 degrees C. While the viability of articular cartilage stored in culture medium is significantly diminished within 1 week, appropriate serology testing to minimize the chances for the disease transmission requires a minimum of 2 weeks. (-)-Epigallocatechin-3-O-gallate (EGCG) has differential effects on the proliferation of cancer and normal cells, thus a cytotoxic effect on various cancer cells, but a cytopreservative effect on normal cells. Therefore, a storage solution containing EGCG might extend the storage duration of articular cartilages. Rabbit osteochondral allografts were performed with osteochondral grafts stored at 4 degrees C in culture medium containing EGCG for 2 weeks and then the clinical effects were examined with macroscopic and histological assessment after 4 weeks. The cartilaginous structure of an osteochondral graft stored with EGCG was well-preserved with high cell viability and glycosaminoglycan (GAG) content of the extracellular matrix (ECM). After an osteochondral allograft, the implanted osteochondral grafts stored with EGCG also provided a significantly better retention of the articular cartilage with viability and metabolic activity. These data suggest that EGCG can be an effective storage agent that allows long-term preservation of articular cartilage under cold storage conditions.
journal_name
Cell Transplantjournal_title
Cell transplantationauthors
Bae JY,Matsumura K,Wakitani S,Kawaguchi A,Tsutsumi S,Hyon SHdoi
10.1177/096368970901805-604subject
Has Abstractpub_date
2009-01-01 00:00:00pages
505-12issue
5eissn
0963-6897issn
1555-3892journal_volume
18pub_type
杂志文章abstract::Mesenchymal stem cells (MSCs) hold great promise for bone regeneration. However, the power of mesenchymal stem cells has not been applied to structural bone allografts in clinical practice. This study designed a new strategy to enhance the efficiency of allografts for segmental bone regeneration. Isolated MSCs were cu...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368909788809839
更新日期:2009-01-01 00:00:00
abstract::Hepatocyte transplantation and artificial organ hepatic support require a number of functionally mature hepatocytes. However, their growth activity and functional behaviors are much smaller in culture after isolation from the liver. We examined whether continuously differentiating hepatocytes from multipotent hepatic ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000003108747028
更新日期:2003-01-01 00:00:00
abstract::It is difficult to normalize plasma glucose for a prolonged period of time by s.c. injection in experimental animals. The goal of this study was to determine the feasibility and the dosage of insulin needed to maintain 24-h normoglycemia in streptozotocin-diabetic Syrian hamsters with a s.c.-implanted osmotic minipump...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368979900800511
更新日期:1999-09-01 00:00:00
abstract::Human skin contains keratinocytes in the epidermis. Such cells share their ectodermal origin with the central nervous system (CNS). Recent studies have demonstrated that terminally differentiated somatic cells can adopt a pluripotent state, or can directly convert its phenotype to neurons, after ectopic expression of ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689720978219
更新日期:2021-01-01 00:00:00
abstract::The choice of enzyme blend is critical for successful islet isolation. Islet yield, viability, integrity, and function are important factors that influence the outcome of islet transplantation. Liberase HI has been used as a standard enzyme for pancreas digestion and has successfully produced islets that reversed dia...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368911X623826
更新日期:2012-01-01 00:00:00
abstract::This invited concise review was written for the special issue of Cell Transplantation to celebrate the 25th anniversary of the American Society for Neural Therapy and Repair (ASNTR). I aimed to present a succinct summary of two interweaved lines of research work carried out by my team members and collaborators over th...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689719850088
更新日期:2019-04-01 00:00:00
abstract::Granulocyte colony-stimulating factor (G-CSF) mobilizes peripheral blood stem cells (PBSCs) derived from bone marrow. We hypothesized that intraspinal transplantation of PBSCs mobilized by G-CSF could promote functional recovery after spinal cord injury. Spinal cords of adult nonobese diabetes/severe immunodeficiency ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368915X688146
更新日期:2016-01-01 00:00:00
abstract::Although cells isolated from fetal liver are one of the major sources for liver tissue engineering, it is still very difficult to induce them to fully differentiate in vitro into mature hepatocytes. We therefore investigated the effects of nicotinamide (NA), dimethyl sulfoxide (DMSO), and oncostatin M (OSM) on differe...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
abstract::The revascularization of islets of Langerhans transplanted in heterotopic sites like the liver by portal vein embolization or the renal subcapsular space is a major process necessary for the viability of grafted cells. This process has been extensively studied by different techniques and the results have shown that is...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1016/s0963-6897(97)00099-7
更新日期:1997-11-01 00:00:00
abstract::Pancreatic beta cell lines may offer an abundant source of cells for beta-cell replacement in type I diabetes. Using regulatory elements of the bacterial tetracycline (tet) operon for conditional expression of SV40 T antigen oncoprotein in transgenic mouse beta cells, we have shown that reversible immortalization is a...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
abstract::Mouse embryonal carcinoma cell line P19 could be differentiated to neurons or glial cells by appropriate induction. We introduced the lacZ gene driven by a calmodulin promoter into P19 cells, and cloned the transfectant CaM-Z that showed the strong expression of lacZ gene after the induction of differentiation. CaM-Z ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1016/0963-6897(96)00030-9
更新日期:1996-09-01 00:00:00
abstract::Rare cells are present in human umbilical cord blood that do not express the hematopoietic marker CD45 and in culture do not produce cells of hematopoietic lineage. These umbilical cord multipotent stem cells (UC-MC) behave as multilineage progenitor cells (stem cells) and can be expanded in tissue culture. Exposure t...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096020197390022
更新日期:2002-04-01 00:00:00
abstract::Gene therapy as well as methods capable of returning cells to a pluripotent state (iPS) have enabled the correction of genetic deficiencies in syngenic adult progenitors, reducing the need for immunosuppression in cell therapy approaches. However, in diseases involving mutations that lead to the complete lack of a pr...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368910X545068
更新日期:2011-01-01 00:00:00
abstract::"Proof-of-principle" that cell replacement therapy works for neurodegeneration has been reported, but only using donor cells collected from fetal brain tissue obtained from surgical terminations of pregnancy. Surgical terminations of pregnancy represent an increasingly limited supply of donor cells due to the tendenc...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368910X546580
更新日期:2011-01-01 00:00:00
abstract::Transplantation of human cells after isolation and culture has become an important alternative for treatment of acute or chronic skin wounds. To increase the efficacy and reduce cost for transplantation of skin cells, more efficient and accurate techniques for evaluation of cell proliferation are needed. Hemocytometer...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000006783982115
更新日期:2006-01-01 00:00:00
abstract::Osteoarthritis (OA) is degenerative disease, leading to pain and functional disability. It is reported that polydeoxyribonucleotide (PDRN) is a suitable therapy for OA. However, the therapeutic mechanisms of PDRN in OA are not fully understood. To investigate the effect of PDRN in an in vitro model of OA, interleukin ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689718804130
更新日期:2018-11-01 00:00:00
abstract::Peripheral blood samples from HIV-seropositive individuals enrolled in a pilot clinical trial investigating the use of allogeneic dendritic cell therapy were evaluated for mixed chimerism. In this study, dendritic cells from HLA-identical, HIV-seronegative siblings were used. Patients received an infusion of dendritic...
journal_title:Cell transplantation
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:2000-05-01 00:00:00
abstract::ET-Kyoto solution (ET-K) is an extracellular-type organ preservation solution containing the cytoprotective disaccharide, trehalose. A previous study reported the supplement of dibutyryl cyclic adenosine monophosphate (db-cAMP) in conventional ET-K to attenuate lung ischemia-reperfusion injury. In this study, the effi...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000008783906928
更新日期:2008-01-01 00:00:00
abstract::Liver transplantation is the only existing modality for treating decompensated liver cirrhosis. Several factors, such as nonavailability of donors, combined with operative risks, complications associated with rejection, usage of immunosuppressive agents, and cost intensiveness, make this strategy available to only a f...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368910X498241
更新日期:2010-01-01 00:00:00
abstract::Critical length nerve defects in the rat sciatic nerve model were reconstructed with chitosan nerve guides filled with Schwann cells (SCs) containing hydrogel. The transplanted SCs were naive or had been genetically modified to overexpress neurotrophic factors, thus providing a cellular neurotrophic factor delivery sy...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368915X688010
更新日期:2016-01-01 00:00:00
abstract::Despite the high prevalence and devastating outcome, there remain a few options for treatment of ischemic stroke. Currently available treatments are limited by a short time window for treatment and marginal efficacy when used. We have tested a human umbilical cord blood-derived stem cell line that has been shown to re...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368916X691277
更新日期:2016-01-01 00:00:00
abstract::In previous studies we demonstrated that transplantation of microencapsulated hepatocytes could correct congenital hyperbilirubinemia in Gunn rats for 4 to 6 wks. Reduction in hyperbilirubinemia followed a single transplantation of isolated encapsulated hepatocytes (IEH). After 4 to 6 wks of transplantation IEH gradua...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368979200100404
更新日期:1992-01-01 00:00:00
abstract::Hepatocyte transplantation is an alternative therapy to orthotopic liver transplantation for the treatment of liver diseases. However, the supply of hepatocytes is limited given the shortage of organs available to isolate good-functioning quality cells. Neonatal livers may be a potential source alternative to adult li...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368913X669743
更新日期:2014-01-01 00:00:00
abstract::Prognosis of patients with heart failure remains poor despite improved conventional and interventional treatment regimens. The improvement of neovascularization and repair processes by administration of bone marrow-derived cells modestly improved the recovery after acute myocardial infarction. However, circulating pat...
journal_title:Cell transplantation
pub_type: 杂志文章,随机对照试验
doi:10.3727/096368912X654957
更新日期:2012-01-01 00:00:00
abstract::In human neonates, immature GABAergic interneurons are markedly affected by an excitotoxic insult. While in adults the interest of cell transplantation has been demonstrated in several neurological disorders, few data are available regarding the immature brain. The low survival rate constitutes a strong limitation in ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368913X670174
更新日期:2014-01-01 00:00:00
abstract::Several previous studies, suggesting the potential use of embryonic xenografts in the treatment of neurological disorders, indicate that neural growth and axonal guidance factors may function across species. In this light, blocks of fetal porcine neocortex were grafted into small cortical lesion cavities made in newbo...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000003108747343
更新日期:2003-01-01 00:00:00
abstract::Human marrow stromal cells (hMSCs) provide functional benefit in rats subjected to stroke. Astrocytes are coupled into a cellular network via gap junction channels, predominantly composed of connexin-43 (Cx43) proteins. Astrocytes are believed to play a vital role in neuroprotection by providing energy substrates to n...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000005783983205
更新日期:2005-01-01 00:00:00
abstract::The requirement for more effective cryopreservation (CP) methodologies in support of the emerging fields of cell bioprocessing and cell therapy is now critical. Current CP strategies appropriately focus on minimizing the damaging actions of physicochemical stressors and membrane disruption associated with extra- and i...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
abstract::Human amniotic membrane (hAM) is the innermost layer of fetal membranes, which surrounds the developing fetus and forms the amniotic cavity. hAM and hAM-derived cells possess many properties that make them suitable for use in regenerative medicine, such as low immunogenicity, promotion of epithelization, anti-inflamma...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689717725528
更新日期:2018-01-01 00:00:00
abstract::We attempted to regenerate bone in a significant osseous defect with various stem cells from deciduous teeth, extracted from puppies, and grafted them into a parent canine mandible as an allograft, parent dental pulp, and bone marrow by tissue engineering and regenerative medicine technology using platelet-rich plasm...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368910X539128
更新日期:2011-01-01 00:00:00