Abstract:
:Mouse embryonal carcinoma cell line P19 could be differentiated to neurons or glial cells by appropriate induction. We introduced the lacZ gene driven by a calmodulin promoter into P19 cells, and cloned the transfectant CaM-Z that showed the strong expression of lacZ gene after the induction of differentiation. CaM-Z cells after differentiation were then implanted in newborn rats or mice intracranially. PCR amplification of the lacZ gene and histological examination of the recipient animals revealed that transplanted cells survived for 1 to 7 mo in the rat or mouse brains with lacZ gene expression and neuronal differentiation. The rate of the successful transplantation was higher in mice than in rats.
journal_name
Cell Transplantjournal_title
Cell transplantationauthors
Watanabe R,Takase-Yoden S,Ikeda T,Atsumi Tdoi
10.1016/0963-6897(96)00030-9subject
Has Abstractpub_date
1996-09-01 00:00:00pages
S9-12issue
5 Suppl 1eissn
0963-6897issn
1555-3892pii
0963689796000309journal_volume
5pub_type
杂志文章abstract::Efficient repopulation by transplanted hepatocytes in the severely injured liver is essential for their clinical application in the treatment of acute hepatic failure. We studied here whether and how the transplanted hepatocytes are able to efficiently repopulate the toxin-induced acute injured liver. Male dipeptidyl ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368909X12483162196647
更新日期:2009-01-01 00:00:00
abstract::Cell therapy has attracted considerable interest as a promising therapeutic alternative for patients with Parkinson's disease (PD). Clinical studies have shown that grafted fetal neural tissue can achieve considerable biochemical and clinical improvements in PD. However, the source of fetal tissue grafts is limited an...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368916X691682
更新日期:2016-11-01 00:00:00
abstract::After a unilateral striatal lesion, animals have generally been seen to have a bilateral impairment in paw reaching, with the contralateral paw being more affected. However, most studies to date have not used a pretraining paradigm to assess maximal capacity for paw reaching, to compare with any lesion-induced loss. T...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000003108746812
更新日期:2003-01-01 00:00:00
abstract::ZNF281 is one of the core transcription factors in embryonic stem cells (ESCs) and has activation and repression roles in the transcription of ESC genes. A known target molecule of Zfp281 (the mouse homologue of ZNF281) is Nanog. However, NANOG is not expressed in most human multipotent stem cells (hMSCs). Here, we in...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368912X654948
更新日期:2013-01-01 00:00:00
abstract::There are conflicting reports on the survival and immune reaction to allografts and xenografts of cultured skin substitutes (CSS). In this study, we investigated the allogeneic and xenogeneic responses to CSS of human keratinocytes and genetically engineered CSS expressing keratinocyte growth factor (KGF) that forms a...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000004783983594
更新日期:2004-01-01 00:00:00
abstract::Xenotransplantation of porcine fetal ventral mesencephalic (pfVM) cells to overcome the dopamine shortage in the striatum of patients with Parkinson's disease seems a viable alternative to allotransplantion of human fetal donor tissue, especially because the latter is complicated by both practical and ethical issues. ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000006783981846
更新日期:2006-01-01 00:00:00
abstract::This study examined how the difference in pore size of porous scaffolds affected the in vitro chondrogenic differentiation of seeded adipose stem cells (ASCs) and the in vivo cartilage repair of ASC/scaffold construct. ASCs were isolated from 18 rabbits and seeded in a porous poly (ε-caprolactone) (PCL) scaffold with ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368912X638865
更新日期:2012-01-01 00:00:00
abstract::Islet transplantation as a therapy or cure for type 1 diabetes has significant promise but has been limited by islet mass requirements and long-term graft failure. The intrahepatic and intravascular site may be responsible for significant loss of transplanted islets. Nonencapsulating biomaterial scaffolds provide a st...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368912X636966
更新日期:2013-01-01 00:00:00
abstract::Since donor T-cells' allorecognition of host antigens is a prerequisite for the onset of graft-versus-host disease (GVHD), blocking their cellular signaling pathways can decrease the severity of GVHD. We hypothesized that epigallocatechin-3-gallate (EGCG), due to its strong affinity to macromolecules, would adhere to...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368911X623934
更新日期:2012-01-01 00:00:00
abstract::The development of new cell replacement strategies using neural stem cells (NSC) may provide an alternative and unlimited cell source for clinical neural transplantation in neurodegenerative diseases such as Parkinson's and Huntington's disease. The clinical application of neural transplantation using NSC will therefo...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000006783464426
更新日期:2006-01-01 00:00:00
abstract::Cerebral palsy (CP) is a common disability which results in permanent chronic motor disability appearing in early childhood. Recently human umbilical cord blood mesenchymal stem cell (hUCB-MSC) infusion has emerged as a promising therapeutic strategy for CP, and the treatment efficacy remains to be confirmed by clinic...
journal_title:Cell transplantation
pub_type: 杂志文章,随机对照试验
doi:10.1177/0963689717729379
更新日期:2018-02-01 00:00:00
abstract::The use of biomaterials has been demonstrated as a viable strategy to promote cell survival and cardiac repair. However, limitations on combinational cell-biomaterial therapies exist, as cellular behavior is influenced by the microenvironment and physical characteristics of the material. Among the different scaffolds ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368915X689794
更新日期:2016-01-01 00:00:00
abstract::The development of cell- and gene-based strategies for regenerative medicine offers a therapeutic option for the repair and potential regeneration of damaged cardiac tissue post-myocardial infarction (MI). Human umbilical cord subepithelial cell-derived stem cells (hUC-SECs), human bone marrow-derived mesenchymal stem...
journal_title:Cell transplantation
pub_type: 杂志文章,评审
doi:10.3727/096368916X691501
更新日期:2016-01-01 00:00:00
abstract::As an alternative to drug immunosuppression, attempts at inducing donor-specific tolerance by intrathymic (IT) inoculations to transplant recipient of donor origin alloantigenic products has proven very promising. Using fiber optic thoracoscopy, a technique for the study of this phenomena was developed for the dog. We...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368979400300413
更新日期:1994-07-01 00:00:00
abstract::Adoptive cell transfer (ACT) of antigen (Ag)-specific CD8(+) cytotoxic T lymphocytes (CTLs) is a highly promising treatment for a variety of diseases. Naive or central memory T-cell-derived effector CTLs are optimal populations for ACT-based immunotherapy because these cells have a high proliferative potential, are le...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368916X690467
更新日期:2016-01-01 00:00:00
abstract::Mechanical thrombectomy (MT) is effective in managing patients with acute ischemic stroke (AIS) caused by large-vessel occlusions and allows for valuable histological analysis of thrombi. However, whether bridging therapy (pretreatment with intravenous thrombolysis before MT) provides additional benefits in patients w...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689718823206
更新日期:2019-06-01 00:00:00
abstract::Testis-derived Sertoli cells have been used to create an immune "privileged" site outside of the testis to facilitate cell transplantation protocols for diabetes and neurodegenerative diseases. In addition to secreting immunoprotective factors, Sertoli cells also secrete growth and trophic factors that appear to enhan...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1016/s0963-6897(97)00022-5
更新日期:1997-03-01 00:00:00
abstract::A human embryonic stem cell (HESC) line, H1, was studied after differentiation to a dopaminergic phenotype in vitro in order to carry out in vivo studies in Parkinsonian monkeys. To identify morphological characteristics of transplanted donor cells, HESCs were transfected with a GFP lentiviral vector. Gene expression ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368913X664865
更新日期:2014-01-01 00:00:00
abstract::Osteoarthritis (OA) is degenerative disease, leading to pain and functional disability. It is reported that polydeoxyribonucleotide (PDRN) is a suitable therapy for OA. However, the therapeutic mechanisms of PDRN in OA are not fully understood. To investigate the effect of PDRN in an in vitro model of OA, interleukin ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689718804130
更新日期:2018-11-01 00:00:00
abstract::Porcine islets show notoriously low insulin secretion levels in response to glucose stimulation. While this is somehow expected in the case of immature islets isolated from fetal and neonatal pigs, disappointingly low secretory responses are frequently reported in studies using in vitro-maturated fetal and neonatal is...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368916X693798
更新日期:2017-05-09 00:00:00
abstract::Heart failure continues to be a major health care concern with relatively few options for severely advanced heart failure patients. The hallmark of heart failure is the progressive dilatation of the left ventricle, thinning of the left ventricular wall leading to increased wall stress and increased myocardial oxygen c...
journal_title:Cell transplantation
pub_type: 临床试验,杂志文章
doi:10.3727/096368911X637461
更新日期:2013-01-01 00:00:00
abstract::We here present an immunologic head-to-head comparison between human umbilical cord lining mesenchymal stem cells (clMSCs) and adult bone marrow MSCs (bmMSCs) from patients >65 years of age. clMSCs had significantly lower HLA class I expression, higher production of tolerogenic TGF-β and IL-10, and showed significantl...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368910X536473
更新日期:2011-01-01 00:00:00
abstract::Wharton's jelly-derived mesenchymal stromal cells (WJ-MSCs) have distinct immunomodulatory and protective effects against kidney, liver, or heart injury. Limited studies have shown that WJ-MSCs attenuates oxygen-glucose deprivation-mediated inflammation in hippocampal slices. The neuroprotective effect of intracerebra...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689718802754
更新日期:2018-11-01 00:00:00
abstract::Human skin contains keratinocytes in the epidermis. Such cells share their ectodermal origin with the central nervous system (CNS). Recent studies have demonstrated that terminally differentiated somatic cells can adopt a pluripotent state, or can directly convert its phenotype to neurons, after ectopic expression of ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689720978219
更新日期:2021-01-01 00:00:00
abstract::Gastric cancer (GC) is a leading cause of cancer-related death with poor prognosis. Growing evidence has shown that long noncoding ribonucleic acid (lncRNA) FEZ family zinc finger 1 antisense RNA 1(FEZF1-AS1), an "oncogene," regulates tumor progression and supports cancer stem cell. However, the tumorigenic mechanism ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689720925059
更新日期:2020-01-01 00:00:00
abstract::Myoblast transplantation is being considered as a potential strategy to improve muscle function in myopathies; hence, it is important to identify the transplanted cells and to have available efficient reagents to track these cells. We first validated a human to mouse xenotransplantation model warranting the complete a...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368908786991588
更新日期:2008-01-01 00:00:00
abstract::Despite recent advances and promising results in children, liver cell transplantation (LCT) should still be regarded as an experimental therapy. Several substantial complications are known from animal studies and individual patients. However, safety data on liver cell infusion in children are scarce. We used LCT in fo...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368909X485058
更新日期:2010-01-01 00:00:00
abstract::The common use of the marrow autograft mononuclear cell (MNC) count derives from positive correlative studies following allogeneic transplantation and from earlier conflicting data regarding the value of the bone marrow autograft colony-forming unit granulocyte-macrophage (CFU-GM) assay for prediction hematologic reco...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:
更新日期:1997-09-01 00:00:00
abstract::Periosteal autografts have the potential to regenerate articular cartilage defects, but this potential is limited by the patient's age. Allograft transplantation from a young donor to an older recipient might bypass this limitation. The effect of the time delay, between death and harvesting of a periosteal graft, on t...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368979900800607
更新日期:1999-11-01 00:00:00
abstract::Potential labels for identifying embryonic raphe neurons and a clonal, neuronally differentiating, raphe-derived cell line, RN33B, in CNS transplantation studies were tested by first characterizing the labels in vitro. The labels that were tested included 4',6-diamidino-2-phenylindole hydrochloride, 1,1'-dioctadecyl-3...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368979300200207
更新日期:1993-03-01 00:00:00