Abstract:
:Transplanted embryonic stem (ES) cells, following myocardial infarction (MI), contribute to limited cardiac repair and regeneration with improved function. Therefore, novel strategies are still needed to understand the effects of genetically modified transplanted stem cells on cardiac remodeling. The present study evaluates whether transplanted mouse ES cells overexpressing TIMP-1, an antiapoptotic and antifibrotic protein, can enhance cardiac myocyte differentiation, inhibit native cardiac myocyte apoptosis, reduce fibrosis, and improve cardiac function in the infarcted myocardium. MI was produced in C57BL/6 mice by coronary artery ligation. TIMP-1-ES cells, ES cells, or culture medium (control) were transplanted into the peri-infarct region of the heart. Immunofluorescence, TUNEL staining, caspase-3 activity, ELISAs, histology, and echocardiography were used to identify newly differentiated cardiac myocytes and assess apoptosis, fibrosis, and heart function. Two weeks post-MI, significantly (p < 0.05) enhanced engraftment and cardiac myocyte differentiation was observed in TIMP-1-ES cell-transplanted hearts compared with hearts transplanted with ES cells and control. Hearts transplanted with TIMP-1-ES cells demonstrated a reduction in apoptosis as well as an increase (p< 0.05) in p-Akt activity compared with ES cells or culture media controls. Infarct size and interstitial and vascular fibrosis were significantly (p< 0.05) decreased in the TIMP-1-ES cell group compared to controls. Furthermore, MMP-9, a key profibrotic protein, was significantly (p < 0.01) reduced following TIMP-1-ES cell transplantation. Echocardiography data showed fractional shortening and ejection fraction were significantly (p< 0.05) improved in the TIMP-1-ES cell group compared with respective controls. Our data suggest that transplanted ES cells overexpressing TIMP-1 attenuate adverse myocardial remodeling and improve cardiac function compared with ES cells that may have therapeutic potential in regenerative medicine.
journal_name
Cell Transplantjournal_title
Cell transplantationauthors
Glass C,Singla DKdoi
10.3727/096368911X627561subject
Has Abstractpub_date
2012-01-01 00:00:00pages
1931-44issue
9eissn
0963-6897issn
1555-3892pii
ct2381glassjournal_volume
21pub_type
杂志文章abstract::Cells derived from the placenta have become the focus of extensive research concerning their ability to be used for regenerative medicine or cellular therapies. In a previous study, we characterized amnion-derived multipotent progenitor cells, or AMP cells, by in vitro methods and showed they were able to inhibit anti...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368913X670165
更新日期:2014-01-01 00:00:00
abstract::Spinal cord injury (SCI) causes loss of neural functions below the level of the lesion due to interruption of spinal pathways and secondary neurodegenerative processes. The transplant of neural stem cells (NSCs) is a promising approach for the repair of SCI. Reprogramming of adult somatic cells into induced pluripoten...
journal_title:Cell transplantation
pub_type: 杂志文章
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abstract::Recently, animal studies have demonstrated the efficacy of endothelial progenitor cell (EPC) therapy for diabetic wound healing. Based on these preclinical studies, we performed a prospective clinical trial phase I/IIa study of autologous G-CSF-mobilized peripheral blood (PB) CD34(+) cell transplantation for nonhealin...
journal_title:Cell transplantation
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journal_title:Cell transplantation
pub_type: 杂志文章,评审
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abstract::In Alzheimer's disease cholinergic neurons degenerate, resulting in loss of hippocampal acetylcholine. The fimbria-fornix aspiration is a well-known animal model mimicking hippocampal cholinergic deficiency. The aim of the present study was to use in vivo lipid-mediated gene transfer to introduce an expression vector ...
journal_title:Cell transplantation
pub_type: 杂志文章
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abstract::Previous observations in heat-shocked pig islets revealed the ambivalent character of the stress response simultaneously inducing processes of protection and apoptosis. To clarify whether the proapoptotic character of the stress response is reduced in heat-exposed islets still embedded in their native environment, hyp...
journal_title:Cell transplantation
pub_type: 杂志文章
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journal_title:Cell transplantation
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journal_title:Cell transplantation
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journal_title:Cell transplantation
pub_type: 临床试验,杂志文章
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更新日期:2013-01-01 00:00:00
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journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1016/0963-6897(95)02026-8
更新日期:1996-05-01 00:00:00
abstract::The purpose of this study was to investigate the clinical efficacy of neural stem/progenitor cell (NS/PC) transplantation to treat severe cortical visual impairment (CVI), a sequela of neonatal brain injury. Fifty-two patients with cerebral injury and CVI were randomly divided into two groups: the treatment group (n =...
journal_title:Cell transplantation
pub_type: 杂志文章,随机对照试验
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更新日期:2013-01-01 00:00:00
abstract::"Proof-of-principle" that cell replacement therapy works for neurodegeneration has been reported, but only using donor cells collected from fetal brain tissue obtained from surgical terminations of pregnancy. Surgical terminations of pregnancy represent an increasingly limited supply of donor cells due to the tendenc...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368910X546580
更新日期:2011-01-01 00:00:00
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journal_title:Cell transplantation
pub_type: 历史文章,杂志文章
doi:10.3727/000000006783982340
更新日期:2006-01-01 00:00:00
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journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689718775382
更新日期:2019-03-01 00:00:00
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journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689719857085
更新日期:2019-08-01 00:00:00
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journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000003783985142
更新日期:2003-01-01 00:00:00
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journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368911X627589
更新日期:2012-01-01 00:00:00
abstract::Possible strategies for treating stroke include: 1) thrombolytic therapy with tissue plasminogen activator (tPA): restoring cerebral blood flow in the acute phase of ischemic stroke but sometimes causing hemorrhagic transformation (HT); 2) stem cell therapy: the repair of disrupted neuronal networks with newly born ne...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368910X532800
更新日期:2011-01-01 00:00:00
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journal_title:Cell transplantation
pub_type: 杂志文章,评审
doi:10.1177/096368970000900213
更新日期:2000-03-01 00:00:00
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journal_title:Cell transplantation
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
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journal_title:Cell transplantation
pub_type: 杂志文章,评审
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doi:10.1177/0963689718780942
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journal_title:Cell transplantation
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doi:10.3727/096368912X655028
更新日期:2012-01-01 00:00:00
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journal_title:Cell transplantation
pub_type: 杂志文章
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更新日期:1997-11-01 00:00:00
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journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368912X653237
更新日期:2013-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2006-01-01 00:00:00
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pub_type: 杂志文章,评审
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journal_title:Cell transplantation
pub_type: 杂志文章
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abstract::To engineer liver tissues with a clinically significant size, in vivo evaluation of performance using large-scale animal studies are necessary before proceeding to human clinical trials. As pigs are the most suitable candidates, the development of culture conditions suitable for porcine hepatocyte progenitors is very ...
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更新日期:2006-01-01 00:00:00
abstract::Xenotransplantation of porcine fetal ventral mesencephalic (pfVM) cells to overcome the dopamine shortage in the striatum of patients with Parkinson's disease seems a viable alternative to allotransplantion of human fetal donor tissue, especially because the latter is complicated by both practical and ethical issues. ...
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更新日期:2006-01-01 00:00:00