Abstract:
:Superparamagnetic iron oxide nanoparticles (MNPs) together with magnetic resonance imaging (MRI) are the preferred tools for monitoring the fate and biodistribution of administered cells in stem cell therapy studies. Commercial MNPs need transfection agents and long incubation times for sufficient cell labeling and further in vivo cell detection. In this work, we have synthesized MNPs coated with pluronic F127 and tetronic 908, and validated their applicability as contrast agents for MRI cell detection on two different cell types: rat mesenchymal stem cells (MSCs) and multipotent neural progenitor cell line from mice (C17.2). No transfection agent was needed for a complete MNP internalization, and the uptake was only dependent on MNP concentration in medium and limited on the incubation time. By combining in vivo MRI and ex vivo histology microscopy, we have demonstrated the MRI signal detected corresponded exclusively to labeled cells and not to free particles. Pluronic F127- and tetronic 908-coated MNPs represent promising contrast agents for stem cell tracking due to their ease of use in preparation, their efficiency for cell labeling, and their high sensitivity for in vivo cell detection.
journal_name
Cell Transplantjournal_title
Cell transplantationauthors
Argibay B,Trekker J,Himmelreich U,Beiras A,Topete A,Taboada P,Pérez-Mato M,Iglesias-Rey R,Sobrino T,Rivas J,Campos F,Castillo Jdoi
10.3727/096368916X691303subject
Has Abstractpub_date
2016-10-01 00:00:00pages
1787-1800issue
10eissn
0963-6897issn
1555-3892pii
content-CT-1553_Argibay_et_aljournal_volume
25pub_type
杂志文章abstract::The diverse requirements for a successful islet encapsulation barrier suggest the benefit of a barrier system that presents differing functionalities to encapsulated cells and host cells. Initially, multifunctional hydrogels were synthesized via the sequential photopolymerization of PEG hydrogel layers, each with diff...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000007783472336
更新日期:2007-11-01 00:00:00
abstract::Apoptosis is a vital pathological factor that accounts for the poor prognosis of traumatic spinal cord injury (t-SCI). The 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFKFB3) is a critical regulator for energy metabolism and proven to have antiapoptotic effects. This study aimed to investigate the neuroprote...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689720950226
更新日期:2020-01-01 00:00:00
abstract::Previously, we found that hepatocyte growth factor receptor (c-Met)-and alpha-fetoprotein (AFP)-expressing cells were present in adult rat bone marrow, and that these cells also expressed hematopoietic stem cell markers, such as CD34, Thy-1, and c-Kit. When bone marrow cells were cultured in a hepatocyte growth medium...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000004783983800
更新日期:2004-01-01 00:00:00
abstract::Mechanical thrombectomy (MT) is effective in managing patients with acute ischemic stroke (AIS) caused by large-vessel occlusions and allows for valuable histological analysis of thrombi. However, whether bridging therapy (pretreatment with intravenous thrombolysis before MT) provides additional benefits in patients w...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689718823206
更新日期:2019-06-01 00:00:00
abstract::Posttransplant infusion of viable donor bone marrow cells (DBMC) has been shown in our previous studies to promote acceptance of incompatible kidney allografts in rhesus monkeys after treatment with polyclonal antithymocyte globulin to deplete peripheral T-lymphocytes. In this nonhuman primate model, the infusion of t...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1016/s0963-6897(97)00005-5
更新日期:1997-07-01 00:00:00
abstract::Previous observations in heat-shocked pig islets revealed the ambivalent character of the stress response simultaneously inducing processes of protection and apoptosis. To clarify whether the proapoptotic character of the stress response is reduced in heat-exposed islets still embedded in their native environment, hyp...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000007783465163
更新日期:2007-01-01 00:00:00
abstract::Currently, different techniques to expand human hepatocytes in vitro are being investigated to generate enough cells for liver-directed cell therapies. However, based on observations in fibroblasts and other cell types, telomere attrition limits the proliferative capacity of normal somatic cells. Therefore, we explore...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000003771000138
更新日期:2003-01-01 00:00:00
abstract::Grafting autologous cultured satellite cells in irreversibly injured rat extensor digitorum longus EDL muscle leads to myofiber regeneration at the grafting site. In this study, we investigated whether cell grafts induced functional improvement and correlated mechanophysiological findings with histological observation...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368979400300103
更新日期:1994-01-01 00:00:00
abstract::Myoblast transplantation is being considered as a potential strategy to improve muscle function in myopathies; hence, it is important to identify the transplanted cells and to have available efficient reagents to track these cells. We first validated a human to mouse xenotransplantation model warranting the complete a...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368908786991588
更新日期:2008-01-01 00:00:00
abstract::A population of Hoechst 33342-stained cells, termed side population (SP) cells, can reconstitute the hematopoietic system of syngeneic mice. This study examined whether limiting numbers of SP cells can repopulate mice across a xenogeneic MHC class I barrier. SP cells were isolated from HLA.B7 and HLA.A2.1 transgenic m...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
abstract::Successful transplantation of cadaver embryonic neural tissue is highly dependent on the method used to obtain the tissue. It is important that the tissue not be contaminated bacteriologically by vaginal flora during the procedure, and that it not be disrupted mechanically. A low-pressure aspiration abortion technique...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368979400300202
更新日期:1994-03-01 00:00:00
abstract::We examined a possible protective effect of the nonpeptidyl low molecular weight radical scavenger IAC [bis(1-hydroxy-2,2,6,6-tetramethyl-4-piperidinyl)decanedioate di-hydrochloride] on isolated human islet cells against isolation and culture oxidative stress. Islets isolated from pancreases of nondiabetic multiorgan ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368908787236639
更新日期:2008-01-01 00:00:00
abstract::The development of cell- and gene-based strategies for regenerative medicine offers a therapeutic option for the repair and potential regeneration of damaged cardiac tissue post-myocardial infarction (MI). Human umbilical cord subepithelial cell-derived stem cells (hUC-SECs), human bone marrow-derived mesenchymal stem...
journal_title:Cell transplantation
pub_type: 杂志文章,评审
doi:10.3727/096368916X691501
更新日期:2016-01-01 00:00:00
abstract::Tumor recurrence is the main limitation of liver transplantation (LT) in patients with hepatocellular carcinoma (HCC) and can be promoted by immunosuppressants. However, there is no prevention or treatment for HCC recurrence after LT. Here we describe a clinical-scale method for an adoptive immunotherapy approach that...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368911X627589
更新日期:2012-01-01 00:00:00
abstract::To investigate potential cures for spinal cord injury (SCI), several researchers have transplanted neural stem/progenitor cells (NS/PCs) into the injured spinal cord by different procedures, including intralesional (IL), intrathecal (IT), and intravenous (IV) injection. However, there are no reports quantifying or com...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368910X536554
更新日期:2011-01-01 00:00:00
abstract::To examine the effects of autologous sural nerve and adrenal medullary tissue intrastriatal cografts upon voluntary motor performance in parkinsonism, a non-human primate 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) model was employed to quantitatively assess skilled hand movements. Motor performance was studie...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1016/0963-6897(95)92155-r
更新日期:1995-01-01 00:00:00
abstract::Sepsis is a life-threatening disease that results in excessive stimulation of the host's immune cells. In the animal study, the purpose was to investigate the roles of fresh frozen plasma (FFP) transfusion in shaping the CD4+ T lymphocytes immune response through modulating the secreted exosome protein Galectin-9 in m...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689720947347
更新日期:2020-01-01 00:00:00
abstract::It is difficult to normalize plasma glucose for a prolonged period of time by s.c. injection in experimental animals. The goal of this study was to determine the feasibility and the dosage of insulin needed to maintain 24-h normoglycemia in streptozotocin-diabetic Syrian hamsters with a s.c.-implanted osmotic minipump...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368979900800511
更新日期:1999-09-01 00:00:00
abstract::This study is a preliminary investigation to determine if bone marrow-derived cells, when implanted into freeze-injured urinary bladders, differentiate into smooth muscle cells and reconstruct smooth muscle layers. Bone marrow cells were harvested from femurs of male ICR mice and cultured in collagen-coated dishes for...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368908784153850
更新日期:2008-01-01 00:00:00
abstract::Spinocerebellar ataxia type 1 (SCA1) is a dominant inherited disease caused by expanded trinucleotide repeats resulting in an increased polyglutamine tract in the gene product. As a potential therapeutic approach for SCA1, we tested antisense RNAs targeting two regions of the ataxin-1 message. Single-stranded regions ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368908786516729
更新日期:2008-01-01 00:00:00
abstract::In this study, we assessed the ability of miR-26b-5p to regulate T cell factor 4 (TCF-4) expression and thereby control human adipose-derived mesenchymal stem cell (hADMSC) adipogenic differentiation. Adipogenic medium was used to induce hADMSC differentiation over a 6-d period. The ability of miR-26b-5p to interact w...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689720934418
更新日期:2020-01-01 00:00:00
abstract::The human neuronal cell-1 (HCN-1) line has recently been established. Under favorable conditions, these cells differentiate into mature neuronal phenotypes. Here we report on further characterization of these cells. Cultured HCN-1 cells express fibronectin immunoreactivity and grow well on fibronectin substrate but do...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368979200100104
更新日期:1992-01-01 00:00:00
abstract::Digital ulcers (DUs) are a rather frequent and invalidating complication in systemic sclerosis (SSc), often showing a very slow or null tendency to heal, in spite of the commonly used systemic and local therapeutic procedures. Recently, stem cell therapy has emerged as a new approach to accelerate wound healing. In th...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368914X685636
更新日期:2015-01-01 00:00:00
abstract::Persistent fetal vasculature (PFV) is a potentially serious developmental anomaly in human eyes, which results from a failure of the primary vitreous and the hyaloid vascular systems to regress during development. Recent findings from our laboratory indicate that fibrovascular membranes harvested from subjects with P...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368912X647153
更新日期:2012-01-01 00:00:00
abstract::Prior studies have suggested the possibility of immune-mediated death of xenogeneic hepatocytes in a bioartificial liver (BAL) during hemoperfusion. This study was designed to elucidate how immunity may cause death of xenogeneic hepatocytes in the BAL. Healthy dogs were treated with a BAL containing hollow fiber membr...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000004783983378
更新日期:2004-01-01 00:00:00
abstract::Liver fibrosis is characterized by excessive accumulation of extracellular matrix components in the liver parenchyma that distorts the normal architecture and hepatic function. Progressive fibrosis could end in the advanced stage known as cirrhosis, resulting in the need to resort to liver transplantation. Amniotic me...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368916X692645
更新日期:2016-12-13 00:00:00
abstract::Islet-like cell clusters generated from human fetal pancreases between 18-24 wk gestation were able to grow and mature both morphologically and functionally after transplantation under the kidney capsule of athymic nude mice. Grafted tissue, predominantly ductal in appearance was unresponsive to glucose 2 wk after gra...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368979400300509
更新日期:1994-09-01 00:00:00
abstract::Spinal cord injury (SCI) causes loss of neural functions below the level of the lesion due to interruption of spinal pathways and secondary neurodegenerative processes. The transplant of neural stem cells (NSCs) is a promising approach for the repair of SCI. Reprogramming of adult somatic cells into induced pluripoten...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368916X691312
更新日期:2016-10-01 00:00:00
abstract::The effect of pretreatments of host muscles with metalloproteinases (MMPs) or with notexin on the migration of transplanted myoblasts was investigated. Transgenic TnILacZ mice in which the beta-galactosidase gene is under the control of a quail fast skeletal troponin I gene promoter were used as donors. A polyethylene...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:
更新日期:2000-07-01 00:00:00
abstract::Although hepatic stem cells are believed to exist and play a critical role in developing and regenerating liver, little is known about their cell surface specificity or differentiation capabilities. To make prospective studies of hepatic stem cells possible, we established an in vitro culture system for identification...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00