Easy and Efficient Cell Tagging with Block Copolymer-Based Contrast Agents for Sensitive MRI Detection in Vivo.

Abstract:

:Superparamagnetic iron oxide nanoparticles (MNPs) together with magnetic resonance imaging (MRI) are the preferred tools for monitoring the fate and biodistribution of administered cells in stem cell therapy studies. Commercial MNPs need transfection agents and long incubation times for sufficient cell labeling and further in vivo cell detection. In this work, we have synthesized MNPs coated with pluronic F127 and tetronic 908, and validated their applicability as contrast agents for MRI cell detection on two different cell types: rat mesenchymal stem cells (MSCs) and multipotent neural progenitor cell line from mice (C17.2). No transfection agent was needed for a complete MNP internalization, and the uptake was only dependent on MNP concentration in medium and limited on the incubation time. By combining in vivo MRI and ex vivo histology microscopy, we have demonstrated the MRI signal detected corresponded exclusively to labeled cells and not to free particles. Pluronic F127- and tetronic 908-coated MNPs represent promising contrast agents for stem cell tracking due to their ease of use in preparation, their efficiency for cell labeling, and their high sensitivity for in vivo cell detection.

journal_name

Cell Transplant

journal_title

Cell transplantation

authors

Argibay B,Trekker J,Himmelreich U,Beiras A,Topete A,Taboada P,Pérez-Mato M,Iglesias-Rey R,Sobrino T,Rivas J,Campos F,Castillo J

doi

10.3727/096368916X691303

subject

Has Abstract

pub_date

2016-10-01 00:00:00

pages

1787-1800

issue

10

eissn

0963-6897

issn

1555-3892

pii

content-CT-1553_Argibay_et_al

journal_volume

25

pub_type

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