Abstract:
:The estimated frequency of MSCs in BM is about 0.001-0.01% of total nucleated cells. Most commonly, one applied therapeutic cell dose is about 1-5 million MSCs/kg body weight, necessitating a reliable, fast, and safe expansion system. The limited availability of MSCs demands for an extensive ex vivo amplification step to accumulate sufficient cell numbers. Human platelet lysate (PL) has proven to be a safe and feasible alternative to animal-derived serum as supplement for MSC cultivation. We have investigated the functionally closed automated cell culture hollow fiber bioreactor Quantum cell expansion system as an alternative novel tool to conventional tissue flasks for efficient clinical-scale MSC isolation and expansion from bone marrow using PL. Cells expanded in the Quantum system fulfilled MSC criteria as shown by flow cytometry and adipogenic, chondrogenic, and osteogenic differentiation capacity. Cell surface expression of a variety of chemokine receptors, adhesion molecules, and additional MSC markers was monitored for several passages by flow cytometry. The levels of critical media components like glucose and lactate were analyzed. PDGF-AA, PDGF-AB/BB, bFGF, TGF-β1, sICAM-1, sVCAM-1, RANTES, GRO, VEGF, sCD40L, and IL-6 were assessed using a LUMINEX platform. Originally optimized for the use of fetal calf serum (FCS) as supplement and fibronectin as coating reagent, we succeeded to obtain an average of more than 100×10(6) of MSCs from as little as 18.8-28.6 ml of BM aspirate using PL. We obtained similar yields of MSCs/µl BM in the FCS-containing and the xenogen-free expansion system. The Quantum system reliably produces a cellular therapeutic dose in a functionally closed system that requires minimal manipulation. Both isolation and expansion are possible using FCS or PL as supplement. Coating of the hollow fibers of the bioreactor is mandatory when loading MSCs. Fibronectin, PL, and human plasma may serve as coating reagents.
journal_name
Cell Transplantjournal_title
Cell transplantationauthors
Rojewski MT,Fekete N,Baila S,Nguyen K,Fürst D,Antwiler D,Dausend J,Kreja L,Ignatius A,Sensebé L,Schrezenmeier Hdoi
10.3727/096368912X657990subject
Has Abstractpub_date
2013-01-01 00:00:00pages
1981-2000issue
11eissn
0963-6897issn
1555-3892pii
ct0891rojewskijournal_volume
22pub_type
杂志文章abstract::Cell therapy has been shown to be a promising alternative therapy for neurorestoration, and more than 30 different types of cells have been shown to possess some capability to restore lost or damaged neurological functions. Chinese physicians have successfully applied cell therapy to many neurological diseases and dam...
journal_title:Cell transplantation
pub_type: 杂志文章,评审
doi:10.3727/096368913X672082
更新日期:2013-01-01 00:00:00
abstract::The effect of tensile stress on the orientation and phenotype of arterial smooth muscle cells (SMCs) cultured in three-dimensional (3D) type I collagen gels was morphologically investigated. Ring-shaped hybrid tissues were prepared by thermal gelation of a cold mixed solution of type I collagen and SMCs derived from b...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368979400300605
更新日期:1994-11-01 00:00:00
abstract::Addition of rIL-6 to IL-6-dependent B9 cells starved for IL-6 for 16-20 h stimulated a vigorous proliferative response. Glucocorticoids (GCs), in a concentration-dependent manner, inhibited rIL-6-stimulated proliferation of B9 cells This inhibition was specific for the GCs, evident by the capacity of the GCs, dexameth...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:
更新日期:2001-03-01 00:00:00
abstract::Establishing reliable islet potency assay is a critical and unmet issue for clinical islet transplantation. Recently, we reported that islets contained high levels of high mobility group box 1 (HMGB1) and damaged islets released HMGB1 in a mouse model. In this study, we hypothesized that the amount of released HMGB1 c...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368912X640592
更新日期:2012-01-01 00:00:00
abstract::The anatomical specificity of axon growth from fetal pig septal xenografts was studied by transplanting septal cells from E30-35 pig fetuses into cholinergic deafferented (192-IgG-saporin-infused) rats or into aged rats (> 18 months). Cell suspensions (100,000 cells/microl) were injected bilaterally into the dorsal an...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368979900800104
更新日期:1999-01-01 00:00:00
abstract::Traumatic brain injury (TBI), a major cause of mortality and morbidity, affects 10 million people worldwide, with limited treatment options. We have previously shown that (-)-phenserine (Phen), an acetylcholinesterase inhibitor originally designed and tested in clinical phase III trials for Alzheimer's disease, can re...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689719854693
更新日期:2019-09-01 00:00:00
abstract::Direct reprogramming of an adult cell into another differentiated lineage-such as fibroblasts into neurons, cardiomyocytes, or blood cells-without passage through an undifferentiated pluripotent stage is a new area of research that has recently emerged alongside stem cell technology and induced pluripotent stem cell r...
journal_title:Cell transplantation
pub_type: 杂志文章,评审
doi:10.3727/096368912X657477
更新日期:2013-01-01 00:00:00
abstract::Testis-derived Sertoli cells have been used to create an immune "privileged" site outside of the testis to facilitate cell transplantation protocols for diabetes and neurodegenerative diseases. In addition to secreting immunoprotective factors, Sertoli cells also secrete growth and trophic factors that appear to enhan...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1016/s0963-6897(97)00022-5
更新日期:1997-03-01 00:00:00
abstract::Granulocyte colony-stimulating factor (G-CSF) mobilizes peripheral blood stem cells (PBSCs) derived from bone marrow. We hypothesized that intraspinal transplantation of PBSCs mobilized by G-CSF could promote functional recovery after spinal cord injury. Spinal cords of adult nonobese diabetes/severe immunodeficiency ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368915X688146
更新日期:2016-01-01 00:00:00
abstract::Digital ulcers (DUs) are a rather frequent and invalidating complication in systemic sclerosis (SSc), often showing a very slow or null tendency to heal, in spite of the commonly used systemic and local therapeutic procedures. Recently, stem cell therapy has emerged as a new approach to accelerate wound healing. In th...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368914X685636
更新日期:2015-01-01 00:00:00
abstract::Gene therapy as well as methods capable of returning cells to a pluripotent state (iPS) have enabled the correction of genetic deficiencies in syngenic adult progenitors, reducing the need for immunosuppression in cell therapy approaches. However, in diseases involving mutations that lead to the complete lack of a pr...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368910X545068
更新日期:2011-01-01 00:00:00
abstract::Successful transplantation of cadaver embryonic neural tissue is highly dependent on the method used to obtain the tissue. It is important that the tissue not be contaminated bacteriologically by vaginal flora during the procedure, and that it not be disrupted mechanically. A low-pressure aspiration abortion technique...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368979400300202
更新日期:1994-03-01 00:00:00
abstract::To investigate potential cures for spinal cord injury (SCI), several researchers have transplanted neural stem/progenitor cells (NS/PCs) into the injured spinal cord by different procedures, including intralesional (IL), intrathecal (IT), and intravenous (IV) injection. However, there are no reports quantifying or com...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368910X536554
更新日期:2011-01-01 00:00:00
abstract::In Alzheimer's disease cholinergic neurons degenerate, resulting in loss of hippocampal acetylcholine. The fimbria-fornix aspiration is a well-known animal model mimicking hippocampal cholinergic deficiency. The aim of the present study was to use in vivo lipid-mediated gene transfer to introduce an expression vector ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
abstract::Cell-based therapy for the bladder has its beginnings in the 1990s with the successful isolation and culture of bladder smooth muscle cells. Since then, several attempts have been made to artificially implant native cell types and stem cell-derived cells into damaged bladders in the form of single-cell injectables or ...
journal_title:Cell transplantation
pub_type: 杂志文章,评审
doi:10.3727/096368914X685050
更新日期:2015-01-01 00:00:00
abstract::Critical length nerve defects in the rat sciatic nerve model were reconstructed with chitosan nerve guides filled with Schwann cells (SCs) containing hydrogel. The transplanted SCs were naive or had been genetically modified to overexpress neurotrophic factors, thus providing a cellular neurotrophic factor delivery sy...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368915X688010
更新日期:2016-01-01 00:00:00
abstract::We investigated the effects of environmental enrichment (EE) on the function of transplanted adipose stem cells (ASCs) and the combined effect of EE and ASC transplantation on neurobehavioral function in an animal model of chronic hypoxic-ischemic (HI) brain injury. HI brain damage was induced in 7-day-old mice by uni...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368912X662390
更新日期:2013-01-01 00:00:00
abstract::Efficient repopulation by transplanted hepatocytes in the severely injured liver is essential for their clinical application in the treatment of acute hepatic failure. We studied here whether and how the transplanted hepatocytes are able to efficiently repopulate the toxin-induced acute injured liver. Male dipeptidyl ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368909X12483162196647
更新日期:2009-01-01 00:00:00
abstract::Successful repair of damage in cerebral palsy (CP) needs effective clinical interventions other than simply symptomatic treatments. To elucidate the feasibility of using olfactory ensheathing cells (OECs) to treat CP in children and adolescents, we conducted a randomized controlled clinical trial (RCT) on 33 patients....
journal_title:Cell transplantation
pub_type: 杂志文章,随机对照试验
doi:10.3727/096368910X492652
更新日期:2010-01-01 00:00:00
abstract::Spinal cord injury (SCI) causes loss of neural functions below the level of the lesion due to interruption of spinal pathways and secondary neurodegenerative processes. The transplant of neural stem cells (NSCs) is a promising approach for the repair of SCI. Reprogramming of adult somatic cells into induced pluripoten...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368916X691312
更新日期:2016-10-01 00:00:00
abstract::Tumor recurrence is the main limitation of liver transplantation (LT) in patients with hepatocellular carcinoma (HCC) and can be promoted by immunosuppressants. However, there is no prevention or treatment for HCC recurrence after LT. Here we describe a clinical-scale method for an adoptive immunotherapy approach that...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368911X627589
更新日期:2012-01-01 00:00:00
abstract::The aim of this work was to determine the effect of nicotine desensitization on dopamine (DA) release in the dorsal striatum and shell of the nucleus accumbens (NAc) from brain slices. In vitro fast-scan cyclic voltammetry analysis was used to evaluate dopamine release in the dorsal striatum and the NAc shell of Sprag...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689718775382
更新日期:2019-03-01 00:00:00
abstract::Glucocorticoids (GCs) are used as immunosuppressive and antiinflammatory agents in organ transplantation and in treating autoimmune diseases and inflammatory disorders. GCs were shown to exert their antiproliferative effects directly through blockade of certain elements of an early membrane-associated signal transduct...
journal_title:Cell transplantation
pub_type: 杂志文章,评审
doi:10.1016/s0963-6897(98)00042-6
更新日期:1998-11-01 00:00:00
abstract::Current good manufacturing practice (cGMP) islet processing facilities provide an ultraclean environment for the safe production of clinical grade islets for transplantation into immunosuppressed diabetic recipients. The objective of this study was to monitor the rate of microbial contamination in islet products after...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:
更新日期:2007-01-01 00:00:00
abstract::Heart failure continues to be a major health care concern with relatively few options for severely advanced heart failure patients. The hallmark of heart failure is the progressive dilatation of the left ventricle, thinning of the left ventricular wall leading to increased wall stress and increased myocardial oxygen c...
journal_title:Cell transplantation
pub_type: 临床试验,杂志文章
doi:10.3727/096368911X637461
更新日期:2013-01-01 00:00:00
abstract::We have recently reported that small-sized bone marrow cells (BMCs) isolated by counterflow centrifugal elutriation and depleted of lineage markers (Fr25lin(-)) have the capacity to differentiate and contribute to regeneration of injured islets. In this study, we assess some of the characteristics of these cells compa...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368913X676899
更新日期:2015-01-01 00:00:00
abstract::Acute kidney injury (AKI) is a major clinical problem that still has no established treatment. We investigated the efficacy of cultured human peripheral blood mononuclear cells (PBMNCs) for AKI. Ischemia/reperfusion injury (IRI) was used to induce AKI in male nonobese diabetic (NOD/severe combined immunodeficiency) mi...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689717753186
更新日期:2018-03-01 00:00:00
abstract::The limited availability of human embryonic tissue for dopamine cell transplants in Parkinson's patients has led to an increased interest in using xenogeneic donor tissue. Unfortunately, without aggressive immunosuppression, such brain xenografts are rejected by the host immune system. Chronic brain xenograft rejectio...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
abstract::A population of Hoechst 33342-stained cells, termed side population (SP) cells, can reconstitute the hematopoietic system of syngeneic mice. This study examined whether limiting numbers of SP cells can repopulate mice across a xenogeneic MHC class I barrier. SP cells were isolated from HLA.B7 and HLA.A2.1 transgenic m...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
abstract::Since donor T-cells' allorecognition of host antigens is a prerequisite for the onset of graft-versus-host disease (GVHD), blocking their cellular signaling pathways can decrease the severity of GVHD. We hypothesized that epigallocatechin-3-gallate (EGCG), due to its strong affinity to macromolecules, would adhere to...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368911X623934
更新日期:2012-01-01 00:00:00