Abstract:
:Digital ulcers (DUs) are a rather frequent and invalidating complication in systemic sclerosis (SSc), often showing a very slow or null tendency to heal, in spite of the commonly used systemic and local therapeutic procedures. Recently, stem cell therapy has emerged as a new approach to accelerate wound healing. In the present study, we have tentatively treated long-lasting and poorly responsive to traditional therapy SSc-related DUs by implantation of autologous adipose tissue-derived cell (ATDC) fractions. Fifteen patients with SSc having a long-lasting DU in only one fingertip who were unresponsive to intensive systemic and local treatment were enrolled in the study. The grafting procedure consisted of the injection, at the basis of the corresponding finger, of 0.5-1 ml of autologous ATDC fractions, separated by centrifugation of adipose tissue collected through liposuction from subcutaneous abdominal fat. Time to heal after the procedure was the primary end point of the study, while reduction of pain intensity and of analgesic consumption represented a secondary end point. Furthermore, the posttherapy variation of the number of capillaries, observed in the nailfold video capillaroscopy (NVC) exam and of the resistivity in the digit arteries, measured by high-resolution echocolor-Doppler, were also taken into account. A rather fast healing of the DUs was reached in all of the enrolled patients (mean time to healing 4.23 weeks; range 2-7 weeks). A significant reduction of pain intensity was observed after a few weeks (p < 0.001), while the number of capillaries was significantly increased at 3- and 6-month NVC assessment (p < 0.0001 in both cases). Finally, a significant after-treatment reduction of digit artery resistivity was also recorded (p < 0.0001). Even with the limitations related to the small number of patients included and to the open-label design of the study, the observed strongly favorable outcome suggests that local grafting with ATDCs could represent a promising option for the treatment of SSc-related DUs unresponsive to more consolidated therapies.
journal_name
Cell Transplantjournal_title
Cell transplantationauthors
Del Papa N,Di Luca G,Sambataro D,Zaccara E,Maglione W,Gabrielli A,Fraticelli P,Moroncini G,Beretta L,Santaniello A,Sambataro G,Ferraresi R,Vitali Cdoi
10.3727/096368914X685636subject
Has Abstractpub_date
2015-01-01 00:00:00pages
2297-305issue
11eissn
0963-6897issn
1555-3892pii
content-CT-1325_Del_Papa_et_aljournal_volume
24pub_type
杂志文章abstract::Exenatide is an analog of the incretin hormone glucagon-like peptide (GLP-1) that is used for the treatment of T2D for their metabolic effects. In addition to its insulinotropic effects, exenatide increases functional islet mass and improves their survival. Improved outcomes have been reported in recent clinical isle...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368911X576027
更新日期:2012-01-01 00:00:00
abstract::This study examined how the difference in pore size of porous scaffolds affected the in vitro chondrogenic differentiation of seeded adipose stem cells (ASCs) and the in vivo cartilage repair of ASC/scaffold construct. ASCs were isolated from 18 rabbits and seeded in a porous poly (ε-caprolactone) (PCL) scaffold with ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368912X638865
更新日期:2012-01-01 00:00:00
abstract::Procurement of multipotential neuroglial stem cells is possible with the addition of epidermal growth factor (EGF). Stem cells will differentiate into neurons and glia upon the removal of EGF from the culture medium. We have previously characterized the neuronal differentiation of stem cells derived from long-term cul...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1016/0963-6897(95)02043-8
更新日期:1996-03-01 00:00:00
abstract::Porcine neonatal islet-like cell clusters (NICCs) may be an attractive source of insulin-producing tissue for xenotransplantation in type I diabetic patients. We examined the functional and immunohistochemical outcome of the islet grafts in vitro during long-term culture and in vivo after transplantation to athymic nu...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000003783985142
更新日期:2003-01-01 00:00:00
abstract::Stem cell-based therapy shows great potential in stroke patients. Intra-artery infusion exhibits greater biological distribution compared to intravenous delivery. In addition, umbilical cord mesenchymal stem cells (UCMSCs) have several advantages compared with other types of stem cells. The aim of this study was to ev...
journal_title:Cell transplantation
pub_type: 临床试验,杂志文章
doi:10.3727/096368912X658818
更新日期:2013-01-01 00:00:00
abstract::The present study aimed to elucidate the mechanisms by which hydrogen sulfide (H2S) attenuates left ventricular remodeling after myocardial infarction (MI). MI was created in mice by left coronary artery ligation. One group of mice received injections of the H2S donor sodium hydrosulfide (NaHS) immediately before and ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368917X695029
更新日期:2017-05-09 00:00:00
abstract::Expression of a fluorescent reporter gene has been studied using two alternate promoters to transcribe the green fluorescent protein (gfp) from Aequorea victoria. The human cytomegalovirus (CMV) enhancer/ promoter or the human muscle-specific creatine kinase promoter (CKM) were inserted along with the gfp cDNA into a ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1016/0963-6897(95)02026-8
更新日期:1996-05-01 00:00:00
abstract::The objective of this study was to define pretransplant islet culture conditions for optimum tissue engraftment in the rat islet allotransplantation model. Lewis rat islets were cultured in TCM 199/5% fetal calf serum for I day at 37 degrees C, followed by 1 day of culture at 22 degrees C. When islets from single dono...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
abstract::Despite the high prevalence and devastating outcome, there remain a few options for treatment of ischemic stroke. Currently available treatments are limited by a short time window for treatment and marginal efficacy when used. We have tested a human umbilical cord blood-derived stem cell line that has been shown to re...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368916X691277
更新日期:2016-01-01 00:00:00
abstract::Epithelial wound repair assures the recovery of the epithelial barrier after wounding. During wound healing epithelial cells migrate to cover the wound surface. The presented experiments were carried out to compare the migration of human keratinocytes from primary and secondary culture on polystyrene, collagen, and fi...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
abstract::Embedded-culture of pancreatic beta-cells derived from a transgenic mouse insulinoma (MIN6 cells) was studied in vitro and in vivo. The MIN6 cells were enmeshed in an agarose-PVMA-collagen matrix for long-term maintenance. The cells formed islet-like cell clusters (ICCs) in the mixed matrix. When 10 mmol/L nicotinamid...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1016/0963-6897(95)00001-e
更新日期:1995-05-01 00:00:00
abstract::Oligodendrocyte (OL) replacement can be a promising strategy for spinal cord injury (SCI) repair. However, the poor posttransplantation survival and inhibitory properties to axonal regeneration are two major challenges that limit their use as donor cells for repair of CNS injuries. Therefore, strategies aimed at enhan...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368912X661373
更新日期:2013-01-01 00:00:00
abstract::Head and neck squamous cell carcinoma (HNSCC) is a malignant tumor of the upper aerodigestive tract affecting the oral cavity, lips, paranasal sinuses, larynx, and nasopharynx. Proteogenomics combines proteomics and genomics and employs mass spectrometry and high-throughput sequencing technologies to identify novel pe...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689720929308
更新日期:2020-01-01 00:00:00
abstract::Natural immunological tolerance can be induced in certain types of allogeneic liver transplantation in rats. To screen for genes associated with the induction of tolerance, suppression subtractive hybridization was performed in the rat liver transplantation model between a DA donor and PVG recipient combination where ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000008783906955
更新日期:2008-01-01 00:00:00
abstract::Large-scale sterile methods for isolating hepatocytes are desirable for the development of bioartificial liver support systems. In this study the traditional centrifuge method was compared with the use of a Baylor Rapid Autologous Transfusion (BRAT) machine for isolating large quantities of porcine hepatocytes. After ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000005783983007
更新日期:2005-01-01 00:00:00
abstract::Angiopoietin-1 (Ang1) mediates vascular maturation and immune response. Diabetes decreases Ang1 expression and disrupts Ang1/Tie2 signaling activity. Vasculotide is an Ang1 mimetic peptide, and has anti-inflammatory effects. In this study, we test the hypothesis that vasculotide treatment induces neuroprotection and d...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689718791568
更新日期:2018-12-01 00:00:00
abstract::Acute kidney injury (AKI) is a major clinical problem that still has no established treatment. We investigated the efficacy of cultured human peripheral blood mononuclear cells (PBMNCs) for AKI. Ischemia/reperfusion injury (IRI) was used to induce AKI in male nonobese diabetic (NOD/severe combined immunodeficiency) mi...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689717753186
更新日期:2018-03-01 00:00:00
abstract::Mesenchymal stem cells (MSCs) may be of value in regeneration of renal tissue after damage; however, lack of biological knowledge and variability of results in animal models limit their utilization. We studied the effects of MSCs on podocytes in vitro and in vivo utilizing adriamycin (ADR) as a model of renal toxicity...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368908787236567
更新日期:2008-01-01 00:00:00
abstract::Efficient maintenance of the undifferentiated status of embryonic stem cells (ESCs) may be important for preparation of high-quality cell sources that can be successfully used for stem cell research and therapy. Here we tried to identify a compound that can enhance the quality of pluripotent stem cells. Treatment of E...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368914X683511
更新日期:2015-01-01 00:00:00
abstract::Airway epithelial cell injury is a key triggering event to activate allergic airway inflammation, such as asthma. We previously reported that administration of mesenchymal stem cells (MSCs) significantly alleviated allergic inflammation in a mouse model of asthma, and the mmu-miR-21/ACVR2A axis may be involved. Howeve...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689718767159
更新日期:2018-03-01 00:00:00
abstract::Glioblastoma multiforme (GBM) displays high resistance to radiation and chemotherapy, due to the presence of a fraction of GBM stem-like cells (GSLCs), which are thus representing the target for GBM elimination. Since mesenchymal stem cells (MSCs) display high tumor tropism, we examined possible antitumor effects of t...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368915X687787
更新日期:2015-01-01 00:00:00
abstract::Cells derived from the placenta have become the focus of extensive research concerning their ability to be used for regenerative medicine or cellular therapies. In a previous study, we characterized amnion-derived multipotent progenitor cells, or AMP cells, by in vitro methods and showed they were able to inhibit anti...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368913X670165
更新日期:2014-01-01 00:00:00
abstract::The need for chronic immune suppression (IS) is one of the hurdles precluding widespread use of islet cell transplantation to restore glycemic control in patients with type 1 diabetes. We report the case of a healthy nonhuman primate (NHP) treated on and off for over 2.5 years with steroid-free IS, consisting of dacli...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689718823505
更新日期:2019-03-01 00:00:00
abstract::It is widely believed that human embryonic stem (huES) cells may represent a valid alternative to donor pancreata as a source of islets for transplantation. Much is known about the transcription factors whose sequential activation results in the generation of islets during pancreatic development. This knowledge has be...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000006783982359
更新日期:2006-01-01 00:00:00
abstract::Human islet allografts are well known to induce full and sustained remission of hyperglycemia, with complete normalization of key metabolic parameters. Nevertheless, acquiring human islets, even from cadaveric human donor pancreases, remains a significant impediment to successful transplantation therapy for diabetes. ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000006783982223
更新日期:2006-01-01 00:00:00
abstract::Future improvements in the recovery and function of pancreatic islets following cryopreservation will require a more precise quantification of the stresses that occur at each stage of the cryopreservation protocol. Changes in solution osmolality during the addition and dilution of cryoprotectants and during freezing a...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368979900800308
更新日期:1999-05-01 00:00:00
abstract::The present study was designed to evaluate the use of collagen gel loaded with human retinal pigment epithelium (ARPE19) in cellular transfer and to assess its viability within the gel. Collagen solution was prepared by dissolving calfskin in hydrochloric acid to make a final concentration of 2.0 mg/ml and this was mi...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000004783983666
更新日期:2004-01-01 00:00:00
abstract::Gammopathies, multiple myeloma, and amyloidosis are plasma dyscrasias characterized by clonal proliferation and immunoglobulin overproduction. Renal impairment is the most common and serious complication with an incidence of 20-30% patients at the diagnosis. Kidney transplant has not been considered feasible in the pr...
journal_title:Cell transplantation
pub_type: 杂志文章,评审
doi:10.3727/096368915X688560
更新日期:2016-01-01 00:00:00
abstract::Gastric cancer (GC) is a leading cause of cancer-related death with poor prognosis. Growing evidence has shown that long noncoding ribonucleic acid (lncRNA) FEZ family zinc finger 1 antisense RNA 1(FEZF1-AS1), an "oncogene," regulates tumor progression and supports cancer stem cell. However, the tumorigenic mechanism ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689720925059
更新日期:2020-01-01 00:00:00
abstract::In multiple sclerosis (MS), a chronic inflammatory relapsing demyelinating disease, failure to control or repair damage leads to progressive neurological dysfunction and neurodegeneration. Implantation of neural stem cells (NSCs) has been shown to promote repair and functional recovery in the acute experimental autoi...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368911X637443
更新日期:2012-01-01 00:00:00