Abstract：
:The present study was designed to evaluate the use of collagen gel loaded with human retinal pigment epithelium (ARPE19) in cellular transfer and to assess its viability within the gel. Collagen solution was prepared by dissolving calfskin in hydrochloric acid to make a final concentration of 2.0 mg/ml and this was mixed with 10,000 ARPE19 cells/ml. The cell viability in gel was determined using MTT assay. van Gieson stain and proliferating cell nuclear antigen (PCNA) were used to identify the location of collagen and to localize the site of cell proliferation, respectively. The ARPE19 cells in gel appeared to be healthy with a rounded morphology. The optimal collagen concentration was 1.9 mg/ml. When this concentration was used to hold cells for over 12 days, it could be seen that the growth rate was the same between day 2 and day 8 in gel and on plastic. When the cell-loaded gels were transferred onto standard tissue culture plastics, progressive cell migrations over time resembling cell migrations in organotypic explant cultures were observed. Upon intravitreal injection of cell-containing collagen suspension into a rabbit's eye, the gel became suspended within the vitreous a few hours after injection (day 0). However, it became obvious that the gel dispersed and spread around the vitreous even after just 24 h. These cells inside the vitreous were PCNA positive, indicating that the human ARPE19 cells have the capacity to proliferate even after 11 days. The present study demonstrated the potential use of collagen gel as a tool in the transfer of cellular matrix onto other substrates. The results show that the cell seeding number must be critically balanced with the concentration of gel for it to be used as transplant material.
journal_name
Cell Transplantjournal_title
Cell transplantationauthors
Yeung CK,Chiang SW,Chan KP,Lam DS,Pang CPdoi
10.3727/000000004783983666subject
Has Abstractpub_date
2004-01-01 00:00:00pages
585-94issue
5eissn
0963-6897issn
1555-3892journal_volume
13pub_type
杂志文章abstract：:Oligodendrocyte (OL) replacement can be a promising strategy for spinal cord injury (SCI) repair. However, the poor posttransplantation survival and inhibitory properties to axonal regeneration are two major challenges that limit their use as donor cells for repair of CNS injuries. Therefore, strategies aimed at enhan...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368912X661373
更新日期：2013-01-01 00:00:00
abstract：:Previous observations in heat-shocked pig islets revealed the ambivalent character of the stress response simultaneously inducing processes of protection and apoptosis. To clarify whether the proapoptotic character of the stress response is reduced in heat-exposed islets still embedded in their native environment, hyp...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/000000007783465163
更新日期：2007-01-01 00:00:00
abstract：:There has been no gold standard for intraoperative monitoring in carotid endarterectomy (CEA) till now. The purpose of the current study was to investigate the value of near-infrared spectroscopy (NIRS) monitoring in CEA and explore the thresholds for intraoperative cerebral hypoperfusion. Eighty-four consecutive pati...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1177/0963689718817760
更新日期：2019-02-01 00:00:00
abstract：:Endostatin (ES) is a potent inhibitor of angiogenesis and tumor growth. Continuous ES delivery of ES improves the efficacy and potency of the antitumoral therapy. The TheraCyte system is a polytetrafluoroethylene (PTFE) semipermeable membrane macroencapsulation system for implantation of genetically engineered cells s...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368909X480927
更新日期：2010-01-01 00:00:00
abstract：:Schwann cells naturally support axonal regeneration after injury in the peripheral nervous system, and have also shown a significant, albeit limited, ability to support axonal growth and remyelination after grafting to the central nervous system (CNS). It is possible that Schwann cell-induced axonal growth in the CNS ...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1016/s0963-6897(97)00166-8
更新日期：1998-03-01 00:00:00
abstract：:We attempted to regenerate bone in a significant osseous defect with various stem cells from deciduous teeth, extracted from puppies, and grafted them into a parent canine mandible as an allograft, parent dental pulp, and bone marrow by tissue engineering and regenerative medicine technology using platelet-rich plasm...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368910X539128
更新日期：2011-01-01 00:00:00
abstract：:Bone nonunion is a pathological condition in which all bone healing processes have stopped, resulting in abnormal mobility between 2 bone segments. The incidence of bone-related injuries will increase in an aging population, leading to such injuries reaching epidemic proportions. Tissue engineering and cell therapy us...
journal_title：Cell transplantation
pub_type： 杂志文章,评审
doi：10.1177/0963689717721203
更新日期：2017-09-01 00:00:00
abstract：:As a putative marker of cerebral small vessel disease, cerebral microbleeds (CMBs) have been associated with vascular cognitive impairment. Both iron accumulation and amyloid protein precursor (APP) dysregulation are recognized as pathological hallmarks underlying the progression of CMBs, but their cross-talk is not y...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1177/0963689719831707
更新日期：2019-08-01 00:00:00
abstract：:Stem cell transplantation represents a promising therapeutic approach for myocardial ischemia/reperfusion (I/R) injury, where cortical bone-derived stem cells (CBSCs) stand out and hold superior cardioprotective effects on myocardial infarction than other types of stem cells. However, the molecular mechanism underlyin...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1177/0963689718786882
更新日期：2018-08-01 00:00:00
abstract：:Nowadays, cardiac regenerative medicine is facing many limitations because of the complexity to find the most suitable stem cell source and to understand the regenerative mechanisms involved. Mesenchymal stem cells (MSCs) have shown great regenerative potential due to their intrinsic properties and ability to restore ...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368914X685771
更新日期：2015-01-01 00:00:00
abstract：:A large proportion of islets are lost after transplantation partly due to a lack of functional vasculature. Islets revascularize from host tissue but the process takes up to 2 weeks and has been suggested to result in reduced vascular density in engrafted islets. We describe a method for observing and quantifying the ...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/000000007783464993
更新日期：2007-01-01 00:00:00
abstract：:Cells derived from the placenta have become the focus of extensive research concerning their ability to be used for regenerative medicine or cellular therapies. In a previous study, we characterized amnion-derived multipotent progenitor cells, or AMP cells, by in vitro methods and showed they were able to inhibit anti...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368913X670165
更新日期：2014-01-01 00:00:00
abstract：:To overcome common disadvantages of standard cultured epidermal sheet grafts (CEG) we have developed a new technique of transplanting cultured human keratinocytes suspended as single cells in a fibrin-glue matrix (Keratinocyte-fibrin-glue suspension-KFGS). In an athymic mouse model with reproducible standardized full ...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1016/s0963-6897(98)00005-0
更新日期：1998-05-01 00:00:00
abstract：:Embryonic stem (ES) cells have the ability to generate teratomas when transplanted into immunodeficient mice, but conditions affecting the generation remain to be elucidated. Nonhuman primate cynomolgus ES cells were transplanted into immunodeficient mice under different conditions; the number of transplanted cells, p...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368908786991560
更新日期：2008-09-01 00:00:00
abstract：:Electrostimulation represents a well-known trophic factor for different tissues. In vitro electrostimulation of non-stem and stem cells induces myogenic predifferentiation and may be a powerful tool to generate cells with the capacity to respond to local areas of injury. We evaluated the effects of in vivo electrostim...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368912X652977
更新日期：2013-01-01 00:00:00
abstract：:The hypogonadal (HPG) mouse is a mutant that lacks a functional gonadotropin-releasing hormone (GnRH) gene. In this study, female HPG mice received bilateral intrahypothalamic implants of an immortalized GnRH-secreting cell line (GT1-7). Nine mice were tested 42- 65 days after implantation to determine whether these c...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1177/096368979300200309
更新日期：1993-05-01 00:00:00
abstract：:We examined a possible protective effect of the nonpeptidyl low molecular weight radical scavenger IAC [bis(1-hydroxy-2,2,6,6-tetramethyl-4-piperidinyl)decanedioate di-hydrochloride] on isolated human islet cells against isolation and culture oxidative stress. Islets isolated from pancreases of nondiabetic multiorgan ...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368908787236639
更新日期：2008-01-01 00:00:00
abstract：:Testicular germ cell tumors (TGCTs) are highly prevalent in young men aged 20-40 years and are one of the most common lethal solid tumors in men of this age. Due to the current unclear mechanism of tumor development, there is a lack of effective treatment, and therefore in-depth research of the molecular mechanism of ...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1177/0963689720946653
更新日期：2020-01-01 00:00:00
abstract：:The diverse requirements for a successful islet encapsulation barrier suggest the benefit of a barrier system that presents differing functionalities to encapsulated cells and host cells. Initially, multifunctional hydrogels were synthesized via the sequential photopolymerization of PEG hydrogel layers, each with diff...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/000000007783472336
更新日期：2007-11-01 00:00:00
abstract：:Cell transplantation is now being explored as a new therapeutic strategy to restore function in the diseased human central nervous system. Neural grafts show long-term survival and function in patients with Parkinson's disease but the symptomatic relief needs to be increased. Cell transplantation seems justified in pa...
journal_title：Cell transplantation
pub_type： 杂志文章,评审
doi：10.1016/0963-6897(95)00022-p
更新日期：1995-07-01 00:00:00
abstract：:Bone marrow-derived cells have different plastic properties, especially regarding cell fusion, which increases with time and is prompted by tissue injury. Several recessive mutations, including Purkinje Cell Degeneration, affect the number of Purkinje cells in homozygosis; heterozygous young animals have an apparently...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368912X638900
更新日期：2012-01-01 00:00:00
abstract：:The main challenge of cell transplantation as a treatment of myopathies is the large amount of tissue to treat. Intravascular delivery of cells may be an ideal route if proven to be effective and safe. Given the importance of nonhuman primates for preclinical research in transplantation, we tested the intra-arterial i...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368914X685032
更新日期：2014-01-01 00:00:00
abstract：:At present, there are still significant barriers that impede the clinical use of hESCs and iPS cells, including ethics, immunorejection, tumorigenesis from hESCs, and teratoma formation from iPS cells. It is therefore necessary to search for alternative sources of stem cells. WJ-MSCs originate from embryonic epiblasts...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368912X662444
更新日期：2013-01-01 00:00:00
abstract：:A human embryonic stem cell (HESC) line, H1, was studied after differentiation to a dopaminergic phenotype in vitro in order to carry out in vivo studies in Parkinsonian monkeys. To identify morphological characteristics of transplanted donor cells, HESCs were transfected with a GFP lentiviral vector. Gene expression ...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368913X664865
更新日期：2014-01-01 00:00:00
abstract：:Cardiac cell therapy with mesenchymal stem cells (MSCs) represents a promising treatment approach for end-stage heart failure. However, little is known about the underlying mechanisms and the fate of the transplanted cells. The objective of the presented work is to determine the feasibility of magnetic resonance imagi...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368912X657747
更新日期：2013-01-01 00:00:00
abstract：:Antibodies to the ligand for CD40 (CD154) have been shown to exert profound effects on the development of cell-mediated immune responses in mice. The present study shows that an antibody to human CD154 (hCD40L) inhibits in vivo Tetanus toxoid (TT) specific secondary antibody responses in hu-PBL-scid mice, as well as t...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1016/s0963-6897(97)00113-9
更新日期：1998-01-01 00:00:00
abstract：:Pancreatic islet transplantation is limited by shortage of donor organs. Although beta-cell lines could be used, their secretion of insulin is characteristically glucose independent and immunoisolation is required. Here we show that intrasplenic transplantation of encapsulated glucose-responsive mouse insulinoma cells...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/000000005783982990
更新日期：2005-01-01 00:00:00
abstract：:Human islets are subjected to a number of stresses before and during their isolation that may influence their survival and engraftment after transplantation. Apoptosis is likely to be activated in response to these stresses. Apoptosis due to intrinsic stresses is regulated by pro- and antiapoptotic members of the Bcl...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368911X566262
更新日期：2012-01-01 00:00:00
abstract：:There is still a lack of sufficient research on the mechanism behind neurogenic bladder (NB) treatment. The aim of this study was to explore the effect of overexpressed stromal cell-derived factor-1 (SDF-1) secreted by engineered immortalized mesenchymal stem cells (imMSCs) on the NB. In this study, primary bone marro...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1177/0963689720902466
更新日期：2020-01-01 00:00:00
abstract：:Cellular transplantation strategies utilizing intraspinal or intrathecal olfactory ensheathing cells (OECs) have been reported as beneficial for spinal cord injury (SCI). However, there are many disadvantages of these methods, including additional trauma to the spinal cord parenchyma and technical challenges. Therefor...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1177/0963689719883842
更新日期：2019-12-01 00:00:00
