Abstract:
:To overcome common disadvantages of standard cultured epidermal sheet grafts (CEG) we have developed a new technique of transplanting cultured human keratinocytes suspended as single cells in a fibrin-glue matrix (Keratinocyte-fibrin-glue suspension-KFGS). In an athymic mouse model with reproducible standardized full thickness wounds this new technique was compared directly to CEG. Reepithelialization was similar in both groups, but reconstitution of the dermo-epidermal junction zone, as shown by electron microscopy and immunohistochemistry was significantly enhanced by the fibrin-glue suspension technique. The new KFGS technique is earlier available than sheet grafts, is able to transfer actively proliferative single keratinocytes, and simplifies the application.
journal_name
Cell Transplantjournal_title
Cell transplantationauthors
Horch RE,Bannasch H,Kopp J,Andree C,Stark GBdoi
10.1016/s0963-6897(98)00005-0subject
Has Abstractpub_date
1998-05-01 00:00:00pages
309-17issue
3eissn
0963-6897issn
1555-3892pii
S0963689798000050journal_volume
7pub_type
杂志文章abstract::The aim of this study was to evaluate the qualitative change in reparative cartilage after autologous chondrocyte implantation (ACI). Ten knees of 10 patients were studied. The signal intensities of reparative and normal cartilage were evaluated by fat-suppressed three-dimensional spoiled-gradient recalled (FS 3D-SPGR...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000005783982666
更新日期:2005-01-01 00:00:00
abstract::Prairie voles show strong pair bonding with their mating partners, and they demonstrate parental behavior toward their infants, indicating that the prairie vole is a unique animal model for analysis of molecular mechanisms of social behavior. Until a recent study, the signaling pathway of oxytocin was thought to be cr...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368916X690502
更新日期:2016-01-01 00:00:00
abstract::Although hepatic stem cells are believed to exist and play a critical role in developing and regenerating liver, little is known about their cell surface specificity or differentiation capabilities. To make prospective studies of hepatic stem cells possible, we established an in vitro culture system for identification...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
abstract::We transplanted adult whole bone marrow prelabeled with bromodeoxyuridine (BrdU) into the ischemic boundary zone of the adult rat brain at 1 day after 2 h of middle cerebral artery occlusion (MCAo). Approximately 3.3% of 10(6) transplanted bone marrow cells were BrdU reactive at 14 days after MCAo. BrdU-reactive cells...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
abstract::Acute kidney injury (AKI) is a major clinical problem that still has no established treatment. We investigated the efficacy of cultured human peripheral blood mononuclear cells (PBMNCs) for AKI. Ischemia/reperfusion injury (IRI) was used to induce AKI in male nonobese diabetic (NOD/severe combined immunodeficiency) mi...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689717753186
更新日期:2018-03-01 00:00:00
abstract::The transplantation of encapsulated dopamine-secreting cells into the striatum represents one potential means of treating Parkinson's disease. The present study investigated the ability of encapsulated PC12 cells, which are derived from rat pheochromocytoma, to supply L-dopa and dopamine into the primate brain in the ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368970000900519
更新日期:2000-09-01 00:00:00
abstract::Mesenchymal stem cells (MSCs) may be of value in regeneration of renal tissue after damage; however, lack of biological knowledge and variability of results in animal models limit their utilization. We studied the effects of MSCs on podocytes in vitro and in vivo utilizing adriamycin (ADR) as a model of renal toxicity...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368908787236567
更新日期:2008-01-01 00:00:00
abstract::Several types of hair loss result from the inability of hair follicles to initiate the anagen phase of the hair regeneration cycle. Modulating signaling pathways in the hair follicle niche can stimulate entry into the anagen phase. Despite much effort, stem cell-based or pharmacological therapies to activate the hair ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368916X691466
更新日期:2016-01-01 00:00:00
abstract::Heart failure continues to be a major health care concern with relatively few options for severely advanced heart failure patients. The hallmark of heart failure is the progressive dilatation of the left ventricle, thinning of the left ventricular wall leading to increased wall stress and increased myocardial oxygen c...
journal_title:Cell transplantation
pub_type: 临床试验,杂志文章
doi:10.3727/096368911X637461
更新日期:2013-01-01 00:00:00
abstract::Human embryonic stem (hES) cells have the ability to differentiate into a variety of different cell lineages and potentially provide a source of differentiated cells for many therapeutic uses. Here we investigated an efficient method of hepatic differentiation from hES cells. A human ES cell line, KhES-1, was used and...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000006783981305
更新日期:2006-01-01 00:00:00
abstract::Grafting autologous cultured satellite cells in irreversibly injured rat extensor digitorum longus EDL muscle leads to myofiber regeneration at the grafting site. In this study, we investigated whether cell grafts induced functional improvement and correlated mechanophysiological findings with histological observation...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368979400300103
更新日期:1994-01-01 00:00:00
abstract::It is widely believed that human embryonic stem (huES) cells may represent a valid alternative to donor pancreata as a source of islets for transplantation. Much is known about the transcription factors whose sequential activation results in the generation of islets during pancreatic development. This knowledge has be...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000006783982359
更新日期:2006-01-01 00:00:00
abstract::Neuroblastoma, a malignant tumor of the sympathetic nervous system, is an aggressive extracranial tumor in childhood. Long noncoding RNAs (lncRNAs) have been discovered to play a key role in the eukaryotic regulatory gene network and be involved in a wide variety of biological processes. We observed that the expressio...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689720943608
更新日期:2020-01-01 00:00:00
abstract::Stem cell transplantation represents a promising therapeutic approach for myocardial ischemia/reperfusion (I/R) injury, where cortical bone-derived stem cells (CBSCs) stand out and hold superior cardioprotective effects on myocardial infarction than other types of stem cells. However, the molecular mechanism underlyin...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689718786882
更新日期:2018-08-01 00:00:00
abstract::In cartilage tissue engineering using stem cells, it is important to stimulate proliferation and control the differentiation of stem cells to specific lineages. Here we reported a combined technique for articular cartilage repair, consisting of bone marrow mesenchymal stem cells (BMMSCs) transfected with connective ti...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368913X669770
更新日期:2014-04-01 00:00:00
abstract::Glioblastoma multiforme (GBM) displays high resistance to radiation and chemotherapy, due to the presence of a fraction of GBM stem-like cells (GSLCs), which are thus representing the target for GBM elimination. Since mesenchymal stem cells (MSCs) display high tumor tropism, we examined possible antitumor effects of t...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368915X687787
更新日期:2015-01-01 00:00:00
abstract::Efficient maintenance of the undifferentiated status of embryonic stem cells (ESCs) may be important for preparation of high-quality cell sources that can be successfully used for stem cell research and therapy. Here we tried to identify a compound that can enhance the quality of pluripotent stem cells. Treatment of E...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368914X683511
更新日期:2015-01-01 00:00:00
abstract::There is still a lack of sufficient research on the mechanism behind neurogenic bladder (NB) treatment. The aim of this study was to explore the effect of overexpressed stromal cell-derived factor-1 (SDF-1) secreted by engineered immortalized mesenchymal stem cells (imMSCs) on the NB. In this study, primary bone marro...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689720902466
更新日期:2020-01-01 00:00:00
abstract::A human embryonic stem cell (HESC) line, H1, was studied after differentiation to a dopaminergic phenotype in vitro in order to carry out in vivo studies in Parkinsonian monkeys. To identify morphological characteristics of transplanted donor cells, HESCs were transfected with a GFP lentiviral vector. Gene expression ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368913X664865
更新日期:2014-01-01 00:00:00
abstract::Several previous studies, suggesting the potential use of embryonic xenografts in the treatment of neurological disorders, indicate that neural growth and axonal guidance factors may function across species. In this light, blocks of fetal porcine neocortex were grafted into small cortical lesion cavities made in newbo...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000003108747343
更新日期:2003-01-01 00:00:00
abstract::Cell-based therapy for the bladder has its beginnings in the 1990s with the successful isolation and culture of bladder smooth muscle cells. Since then, several attempts have been made to artificially implant native cell types and stem cell-derived cells into damaged bladders in the form of single-cell injectables or ...
journal_title:Cell transplantation
pub_type: 杂志文章,评审
doi:10.3727/096368914X685050
更新日期:2015-01-01 00:00:00
abstract::This study was designed to investigate functional recovery after the transplantation of mesenchymal stem cells (MSCs) or neurally differentiated MSCs (NMSCs) derived from bone marrow in a rat model of spinal cord injury (SCI). Sprague-Dawley rats were subjected to incomplete SCI using an NYU impactor to create a free ...
journal_title:Cell transplantation
pub_type: 杂志文章,已发布勘误
doi:10.3727/096368916X692078
更新日期:2016-07-01 00:00:00
abstract::As an alternative to drug immunosuppression, attempts at inducing donor-specific tolerance by intrathymic (IT) inoculations to transplant recipient of donor origin alloantigenic products has proven very promising. Using fiber optic thoracoscopy, a technique for the study of this phenomena was developed for the dog. We...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368979400300413
更新日期:1994-07-01 00:00:00
abstract:REGISTRATION NUMBER OF CLINICAL TRIALS:ChiCTR1900021317. ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689720931428
更新日期:2020-01-01 00:00:00
abstract::Ischemic stroke remains a serious threat to human life. There are limited effective therapies for the treatment of stroke. We have previously demonstrated that angiogenesis and neurogenesis in the brain play an important role in functional recovery following ischemic stroke. Recent studies indicate that increased arte...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689718771889
更新日期:2018-04-01 00:00:00
abstract::Superparamagnetic iron oxide nanoparticles (MNPs) together with magnetic resonance imaging (MRI) are the preferred tools for monitoring the fate and biodistribution of administered cells in stem cell therapy studies. Commercial MNPs need transfection agents and long incubation times for sufficient cell labeling and fu...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368916X691303
更新日期:2016-10-01 00:00:00
abstract::The long-term durability of agarose microencapsulated islets against autoimmunity was evaluated in NOD mice. Islets were isolated from 6-8-week-old prediabetic male NOD mice and microencapsulated in 5% agarose hydrogel. Microencapsulated or nonencapsulated islets were transplanted into the omental pouch of spontaneous...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000006783981954
更新日期:2006-01-01 00:00:00
abstract::Direct reprogramming of an adult cell into another differentiated lineage-such as fibroblasts into neurons, cardiomyocytes, or blood cells-without passage through an undifferentiated pluripotent stage is a new area of research that has recently emerged alongside stem cell technology and induced pluripotent stem cell r...
journal_title:Cell transplantation
pub_type: 杂志文章,评审
doi:10.3727/096368912X657477
更新日期:2013-01-01 00:00:00
abstract::Gene therapy as well as methods capable of returning cells to a pluripotent state (iPS) have enabled the correction of genetic deficiencies in syngenic adult progenitors, reducing the need for immunosuppression in cell therapy approaches. However, in diseases involving mutations that lead to the complete lack of a pr...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368910X545068
更新日期:2011-01-01 00:00:00
abstract::Hepatocyte-based biological therapies are increasingly envisioned for temporary support in acute liver failure and provision of specific-liver functions in liver-based metabolic deficiency. One of the hurdles to develop such therapies is severe shortage of human livers for hepatocyte isolation. To address the issue, w...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000002783985693
更新日期:2002-07-01 00:00:00