Abstract:
:Grafting autologous cultured satellite cells in irreversibly injured rat extensor digitorum longus EDL muscle leads to myofiber regeneration at the grafting site. In this study, we investigated whether cell grafts induced functional improvement and correlated mechanophysiological findings with histological observations. In cell grafted muscles, the number of myofibers did not differ significantly between 2 wk and 3 mo, whereas no regenerating myofibers were observed in ungrafted controls. During this period, the total number of myofibers in the cell grafted muscles represented 48.2-51.9% of that in normal muscles. The mean diameter of regenerated myofibers increased with time, reaching a maximum (32 microns) at the second mo and remained smaller than that of normal myofibers (47 microns). Muscle function was measured by mechanophysiological recordings of muscle response to supramaximal electrical stimulation of the nerve in situ. Cell grafted muscles exhibited a progressive improvement of all contractile parameters. After 3 mo, a 4-fold increase in absolute values of twitch and tetanic tension outputs was measured in cell grafted muscles when compared to ungrafted controls. However, these parameters remained much lower than in normal muscles (23.4% and 22.3% of control, respectively). This study showed that myogenic cell grafts replace degenerated myofibers and form functional myofibers. Functional improvement observed, between 2 wk and 3 mo after cell grafting, correlated with the development, differentiation, and maturation of the regenerated myofibers rather than with an increase in the number of regenerated myofibers.
journal_name
Cell Transplantjournal_title
Cell transplantationauthors
Alameddine HS,Louboutin JP,Dehaupas M,Sébille A,Fardeau Mdoi
10.1177/096368979400300103subject
Has Abstractpub_date
1994-01-01 00:00:00pages
3-14issue
1eissn
0963-6897issn
1555-3892journal_volume
3pub_type
杂志文章abstract::We have recently reported that small-sized bone marrow cells (BMCs) isolated by counterflow centrifugal elutriation and depleted of lineage markers (Fr25lin(-)) have the capacity to differentiate and contribute to regeneration of injured islets. In this study, we assess some of the characteristics of these cells compa...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368913X676899
更新日期:2015-01-01 00:00:00
abstract::To overcome common disadvantages of standard cultured epidermal sheet grafts (CEG) we have developed a new technique of transplanting cultured human keratinocytes suspended as single cells in a fibrin-glue matrix (Keratinocyte-fibrin-glue suspension-KFGS). In an athymic mouse model with reproducible standardized full ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1016/s0963-6897(98)00005-0
更新日期:1998-05-01 00:00:00
abstract::Pancreas procurement for islet isolation and transplantation is limited by concerns for the detrimental effects of postmortem ischemia. Hypothermic machine perfusion (HMP) preservation technology has had a major impact in circumventing ischemic injury in clinical kidney transplantation and is applied here to the prese...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368910X486316
更新日期:2010-01-01 00:00:00
abstract::Mesenchymal stem cells (MSCs) hold great promise for bone regeneration. However, the power of mesenchymal stem cells has not been applied to structural bone allografts in clinical practice. This study designed a new strategy to enhance the efficiency of allografts for segmental bone regeneration. Isolated MSCs were cu...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368909788809839
更新日期:2009-01-01 00:00:00
abstract::Mechanical thrombectomy (MT) is effective in managing patients with acute ischemic stroke (AIS) caused by large-vessel occlusions and allows for valuable histological analysis of thrombi. However, whether bridging therapy (pretreatment with intravenous thrombolysis before MT) provides additional benefits in patients w...
journal_title:Cell transplantation
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abstract::Radiotherapy is an effective treatment for brain tumors but often results in cognitive deficits in survivors. Transplantation of embryonic or brain-derived neural stem/progenitor cells (BNSPCs) ameliorated cognitive impairment after irradiation (IR) in animal models. However, such an approach in patients requires a cl...
journal_title:Cell transplantation
pub_type: 杂志文章
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更新日期:2014-01-01 00:00:00
abstract::In diabetic patients cardiovascular morbidity and mortality is still a major problem. Our aim was to study the effect of kidney-pancreas transplantation on survival, cardiovascular events, and causes of death in diabetic type 1 uremic patients. Three hundred and thirty-three uremic IDDM patients were enrolled in our w...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368970000900621
更新日期:2000-11-01 00:00:00
abstract::Changes in morphology, metabolism, myosin heavy chain gene expression, and functional performances in damaged rabbit muscles with or without transplantation of primary satellite cells were investigated. For this purpose, we damaged bilaterally the fast muscle tibialis anterior (TA) with either 1.5 or 2.6 ml cardiotoxi...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
abstract::Previously, we found that hepatocyte growth factor receptor (c-Met)-and alpha-fetoprotein (AFP)-expressing cells were present in adult rat bone marrow, and that these cells also expressed hematopoietic stem cell markers, such as CD34, Thy-1, and c-Kit. When bone marrow cells were cultured in a hepatocyte growth medium...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000004783983800
更新日期:2004-01-01 00:00:00
abstract::Electrostimulation represents a well-known trophic factor for different tissues. In vitro electrostimulation of non-stem and stem cells induces myogenic predifferentiation and may be a powerful tool to generate cells with the capacity to respond to local areas of injury. We evaluated the effects of in vivo electrostim...
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pub_type: 杂志文章
doi:10.3727/096368912X652977
更新日期:2013-01-01 00:00:00
abstract::The purpose of this study was to investigate the clinical efficacy of neural stem/progenitor cell (NS/PC) transplantation to treat severe cortical visual impairment (CVI), a sequela of neonatal brain injury. Fifty-two patients with cerebral injury and CVI were randomly divided into two groups: the treatment group (n =...
journal_title:Cell transplantation
pub_type: 杂志文章,随机对照试验
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更新日期:2013-01-01 00:00:00
abstract::Clinically, many candidates for islet transplantation are already immunized, which increases their risk of graft rejection. Encapsulation of pancreatic islets using the TheraCyte™ device has been shown to protect against allograft rejection in nonimmunized recipients. However, the capacity of the TheraCyte™ device to ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368912X657486
更新日期:2013-01-01 00:00:00
abstract::We previously demonstrated that mesenchymal cells from human amniotic membrane (hAMTCs) inhibit the generation and maturation of monocyte-derived dendritic cells (DCs) in vitro. Considering the crucial role of DCs in the immune response and that epithelial cells of the human amniotic membrane (hAECs) share some of the...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368914X684033
更新日期:2015-01-01 00:00:00
abstract::Oligodendrocyte (OL) replacement can be a promising strategy for spinal cord injury (SCI) repair. However, the poor posttransplantation survival and inhibitory properties to axonal regeneration are two major challenges that limit their use as donor cells for repair of CNS injuries. Therefore, strategies aimed at enhan...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368912X661373
更新日期:2013-01-01 00:00:00
abstract::Gene therapy as well as methods capable of returning cells to a pluripotent state (iPS) have enabled the correction of genetic deficiencies in syngenic adult progenitors, reducing the need for immunosuppression in cell therapy approaches. However, in diseases involving mutations that lead to the complete lack of a pr...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368910X545068
更新日期:2011-01-01 00:00:00
abstract::Hematopoietic stem and progenitor cells reside within the bone marrow (BM) microenvironment. By a well-balanced interplay between self-renewal and differentiation, they ensure a lifelong supply of mature blood cells. Physiologically, multiple different cell types contribute to the regulation of stem and progenitor cel...
journal_title:Cell transplantation
pub_type: 杂志文章,评审
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更新日期:2018-05-01 00:00:00
abstract::To learn more about nonimmune-mediated islet graft failure, we transplanted different preparations (preps) of isolated human islets under the kidney capsule of streptozotocin (STZ)-diabetic nude mice. One month after the implantation of 1,000 or 2,000 islets, grafts were harvested for morphological, immunohistochemica...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368908787648137
更新日期:2008-01-01 00:00:00
abstract::Procurement of multipotential neuroglial stem cells is possible with the addition of epidermal growth factor (EGF). Stem cells will differentiate into neurons and glia upon the removal of EGF from the culture medium. We have previously characterized the neuronal differentiation of stem cells derived from long-term cul...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1016/0963-6897(95)02043-8
更新日期:1996-03-01 00:00:00
abstract::Embedded-culture of pancreatic beta-cells derived from a transgenic mouse insulinoma (MIN6 cells) was studied in vitro and in vivo. The MIN6 cells were enmeshed in an agarose-PVMA-collagen matrix for long-term maintenance. The cells formed islet-like cell clusters (ICCs) in the mixed matrix. When 10 mmol/L nicotinamid...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1016/0963-6897(95)00001-e
更新日期:1995-05-01 00:00:00
abstract::In previous work, we engineered functional cell sheets using bone marrow-derived mesenchymal stem cells (BM-MSCs) to promote islet graft survival. In the present study, we hypothesized that a cell sheet using dermal fibroblasts could be an alternative to MSCs, and then we aimed to evaluate the effects of this cell she...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368916X690854
更新日期:2016-01-01 00:00:00
abstract::Investigations indicate that an extract of green tea, polyphenol, can significantly increase the culture survival rate of rat islets without deteriorating their functionality. In this study, we examined the effect of adding polyphenol to islets isolated from human pancreata and nonhuman primate pancreata. Islets were ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000004773301825
更新日期:2004-01-01 00:00:00
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doi:10.1177/0963689718780942
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doi:10.3727/096368912X657747
更新日期:2013-01-01 00:00:00
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journal_title:Cell transplantation
pub_type: 临床试验,杂志文章
doi:10.3727/096368912X639017
更新日期:2013-01-01 00:00:00
abstract::Periosteal autografts have the potential to regenerate articular cartilage defects, but this potential is limited by the patient's age. Allograft transplantation from a young donor to an older recipient might bypass this limitation. The effect of the time delay, between death and harvesting of a periosteal graft, on t...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368979900800607
更新日期:1999-11-01 00:00:00
abstract::Autologous cardiosphere-derived cells (CDCs) were the first therapeutic modality to demonstrate myocardial regeneration with a decrease in scar size and an increase in viable, functional tissue. Widespread applicability of autologous CDC therapy is limited by the need for patient-specific myocardial biopsy, cell proce...
journal_title:Cell transplantation
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.3727/096368916X692933
更新日期:2017-02-16 00:00:00
abstract::Large-scale sterile methods for isolating hepatocytes are desirable for the development of bioartificial liver support systems. In this study the traditional centrifuge method was compared with the use of a Baylor Rapid Autologous Transfusion (BRAT) machine for isolating large quantities of porcine hepatocytes. After ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000005783983007
更新日期:2005-01-01 00:00:00
abstract::Possible strategies for treating stroke include: 1) thrombolytic therapy with tissue plasminogen activator (tPA): restoring cerebral blood flow in the acute phase of ischemic stroke but sometimes causing hemorrhagic transformation (HT); 2) stem cell therapy: the repair of disrupted neuronal networks with newly born ne...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368910X532800
更新日期:2011-01-01 00:00:00
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journal_title:Cell transplantation
pub_type: 杂志文章
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更新日期:2020-01-01 00:00:00
abstract::During the past 15 years, a variety of peptides, known as protein transduction domains (PTDs), or cell-penetrating peptides (CPPs), have been characterized for their ability to translocate into live cells. There are now numerous examples of biologically active full-length proteins and peptides that have been successfu...
journal_title:Cell transplantation
pub_type: 杂志文章,评审
doi:10.3727/096368910X508744
更新日期:2010-01-01 00:00:00