Abstract:
:Electrostimulation represents a well-known trophic factor for different tissues. In vitro electrostimulation of non-stem and stem cells induces myogenic predifferentiation and may be a powerful tool to generate cells with the capacity to respond to local areas of injury. We evaluated the effects of in vivo electrostimulation on infarcted myocardium using a miniaturized multiparameter implantable stimulator in rats. Parameters of electrostimulation were organized to avoid a direct driving or pacing of native heart rhythm. Electrical stimuli were delivered for 14 days across the scar site. In situ electrostimulation used as a cell-free, cytokine-free stimulation system, improved myocardial function, and increased angiogenesis through endothelial progenitor cell migration and production of vascular endothelial growth factor (VEGF). In situ electrostimulation represents a novel means to stimulate repair of the heart and other organs, as well as to precondition tissues for treatment with cell-based therapies.
journal_name
Cell Transplantjournal_title
Cell transplantationauthors
Spadaccio C,Rainer A,De Marco F,Lusini M,Gallo P,Sedati P,Muda AO,De Porcellinis S,Gregorj C,Avvisati G,Trombetta M,Chello M,Covino E,Bull DA,Patel AN,Genovese JAdoi
10.3727/096368912X652977subject
Has Abstractpub_date
2013-01-01 00:00:00pages
493-503issue
3eissn
0963-6897issn
1555-3892pii
ct0480spadacciojournal_volume
22pub_type
杂志文章abstract::Hematopoietic stem and progenitor cells reside within the bone marrow (BM) microenvironment. By a well-balanced interplay between self-renewal and differentiation, they ensure a lifelong supply of mature blood cells. Physiologically, multiple different cell types contribute to the regulation of stem and progenitor cel...
journal_title:Cell transplantation
pub_type: 杂志文章,评审
doi:10.1177/0963689717745890
更新日期:2018-05-01 00:00:00
abstract::A human embryonic stem cell (HESC) line, H1, was studied after differentiation to a dopaminergic phenotype in vitro in order to carry out in vivo studies in Parkinsonian monkeys. To identify morphological characteristics of transplanted donor cells, HESCs were transfected with a GFP lentiviral vector. Gene expression ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368913X664865
更新日期:2014-01-01 00:00:00
abstract::Duchenne muscular dystrophy (DMD) is an inherited disease and a main target of myogenic cell transplantation (MT). After the failure of the first clinical trials with DMD patients, the poor migration of transplanted cells has been suspected to be a major problem for a more effective clinical application of MT. Previou...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368909X470900
更新日期:2009-01-01 00:00:00
abstract::Human islet allografts are well known to induce full and sustained remission of hyperglycemia, with complete normalization of key metabolic parameters. Nevertheless, acquiring human islets, even from cadaveric human donor pancreases, remains a significant impediment to successful transplantation therapy for diabetes. ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000006783982223
更新日期:2006-01-01 00:00:00
abstract::The effect of pretreatments of host muscles with metalloproteinases (MMPs) or with notexin on the migration of transplanted myoblasts was investigated. Transgenic TnILacZ mice in which the beta-galactosidase gene is under the control of a quail fast skeletal troponin I gene promoter were used as donors. A polyethylene...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:
更新日期:2000-07-01 00:00:00
abstract::Development of a tissue-engineered neo-kidney augment (NKA) requires evaluation of defined, therapeutically relevant cell and cell/biomaterial composites (NKA constructs) for regenerative potential in mammalian kidney. Previous work identified primary renal cell populations that extended survival and improved renal f...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368911X566172
更新日期:2011-01-01 00:00:00
abstract::Gastric cancer (GC) is a leading cause of cancer-related death with poor prognosis. Growing evidence has shown that long noncoding ribonucleic acid (lncRNA) FEZ family zinc finger 1 antisense RNA 1(FEZF1-AS1), an "oncogene," regulates tumor progression and supports cancer stem cell. However, the tumorigenic mechanism ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689720925059
更新日期:2020-01-01 00:00:00
abstract::Critical length nerve defects in the rat sciatic nerve model were reconstructed with chitosan nerve guides filled with Schwann cells (SCs) containing hydrogel. The transplanted SCs were naive or had been genetically modified to overexpress neurotrophic factors, thus providing a cellular neurotrophic factor delivery sy...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368915X688010
更新日期:2016-01-01 00:00:00
abstract::The requirement for more effective cryopreservation (CP) methodologies in support of the emerging fields of cell bioprocessing and cell therapy is now critical. Current CP strategies appropriately focus on minimizing the damaging actions of physicochemical stressors and membrane disruption associated with extra- and i...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
abstract::The choice of enzyme blend is critical for successful islet isolation. Islet yield, viability, integrity, and function are important factors that influence the outcome of islet transplantation. Liberase HI has been used as a standard enzyme for pancreas digestion and has successfully produced islets that reversed dia...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368911X623826
更新日期:2012-01-01 00:00:00
abstract::Nonhuman primate models of Parkinson's disease (PD) have been invaluable to our understanding of the human disease and in the advancement of novel therapies for its treatment. In this review, we attempt to give a brief overview of the animal models of PD currently used, with a more comprehensive focus on the advantage...
journal_title:Cell transplantation
pub_type: 杂志文章,评审
doi:10.3727/000000006783982025
更新日期:2006-01-01 00:00:00
abstract::Human islets are subjected to a number of stresses before and during their isolation that may influence their survival and engraftment after transplantation. Apoptosis is likely to be activated in response to these stresses. Apoptosis due to intrinsic stresses is regulated by pro- and antiapoptotic members of the Bcl...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368911X566262
更新日期:2012-01-01 00:00:00
abstract::Ten mongrel dogs were used in this study. Diabetes was chemically induced in 7 dogs, and 3 dogs served as normal controls. For each diabetic dog, 5 million human bone marrow-derived mesenchymal stem cells/kg were differentiated to form insulin-producing cells using a trichostatin-based protocol. Cells were then loaded...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689718759913
更新日期:2018-06-01 00:00:00
abstract::Cell escape occurs after intramyocardial injection for treatment of myocardial infarction (MI) and then the migrated cells might be entrapped by extracardiac organs. We investigated the fate of migrated bone marrow-derived mesenchymal stromal cells (MSCs) and their impact on lung, liver, and spleen. MI model was creat...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368910X513982
更新日期:2010-01-01 00:00:00
abstract::Spinal cord injury (SCI) causes loss of neural functions below the level of the lesion due to interruption of spinal pathways and secondary neurodegenerative processes. The transplant of neural stem cells (NSCs) is a promising approach for the repair of SCI. Reprogramming of adult somatic cells into induced pluripoten...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368916X691312
更新日期:2016-10-01 00:00:00
abstract::Somatic stem cells can be isolated from a variety of sources. Although some studies have suggested that somatic stem cells may represent a cell population that is very similar to embryonic stem (ES) cells, it remains unclear whether somatic stem cells retain the potential to differentiate into any cell type derived fr...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368970901805-614
更新日期:2009-01-01 00:00:00
abstract::The ability to replace damaged myocardial tissue with new striated muscle would constitute a major advance in the treatment of diseases that irreversibly injure cardiac muscle cells. The creation of focal grafts of skeletal muscle has been reported following the intramural injection of skeletal myoblasts into both nor...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1016/0963-6897(95)02016-0
更新日期:1996-01-01 00:00:00
abstract::Tumor recurrence is the main limitation of liver transplantation (LT) in patients with hepatocellular carcinoma (HCC) and can be promoted by immunosuppressants. However, there is no prevention or treatment for HCC recurrence after LT. Here we describe a clinical-scale method for an adoptive immunotherapy approach that...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368911X627589
更新日期:2012-01-01 00:00:00
abstract::In Alzheimer's disease cholinergic neurons degenerate, resulting in loss of hippocampal acetylcholine. The fimbria-fornix aspiration is a well-known animal model mimicking hippocampal cholinergic deficiency. The aim of the present study was to use in vivo lipid-mediated gene transfer to introduce an expression vector ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
abstract::Periosteal autografts have the potential to regenerate articular cartilage defects, but this potential is limited by the patient's age. Allograft transplantation from a young donor to an older recipient might bypass this limitation. The effect of the time delay, between death and harvesting of a periosteal graft, on t...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368979900800607
更新日期:1999-11-01 00:00:00
abstract::Prognosis of patients with heart failure remains poor despite improved conventional and interventional treatment regimens. The improvement of neovascularization and repair processes by administration of bone marrow-derived cells modestly improved the recovery after acute myocardial infarction. However, circulating pat...
journal_title:Cell transplantation
pub_type: 杂志文章,随机对照试验
doi:10.3727/096368912X654957
更新日期:2012-01-01 00:00:00
abstract::Achieving good islet isolation is one of the most important factors for successful islet transplantation. Porcine pancreas is suitable for islet isolation research due to its anatomical and physiological similarities to human pancreas. In this study, we evaluated a new porcine islet isolation method designed to maximi...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000005783982512
更新日期:2005-01-01 00:00:00
abstract::Stem cell-based therapy shows great potential in stroke patients. Intra-artery infusion exhibits greater biological distribution compared to intravenous delivery. In addition, umbilical cord mesenchymal stem cells (UCMSCs) have several advantages compared with other types of stem cells. The aim of this study was to ev...
journal_title:Cell transplantation
pub_type: 临床试验,杂志文章
doi:10.3727/096368912X658818
更新日期:2013-01-01 00:00:00
abstract::In this study the time course of homing and the body distribution of systemically delivered bone marrow mesenchymal stem cells (BM-MSCs) after myocardial infarction (MI) were evaluated. BM-MSCs were isolated from Wistar rats, expanded in vitro, and their phenotypical characterization was performed by flow cytometer. R...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368909X479677
更新日期:2010-01-01 00:00:00
abstract::Endothelial progenitor cells (EPCs) have been applied for cell therapy because of their roles in angiogenesis and neovascularization in ischemic tissue. However, adverse responses caused by EPC therapy have not been fully investigated. In this study, a human peripheral blood sample was collected from a healthy donor a...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689718821686
更新日期:2019-07-01 00:00:00
abstract::Several clinical trials of bone marrow cell infusion in patients with liver cirrhosis (LC) have shown clinical improvement, despite conflicting results from animal models. We investigated serial pathological features and the clinical impact after autologous bone marrow infusion (ABMI) in patients with advanced LC. Ten...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368910X506863
更新日期:2010-01-01 00:00:00
abstract::ET-Kyoto solution (ET-K) is an extracellular-type organ preservation solution containing the cytoprotective disaccharide, trehalose. A previous study reported the supplement of dibutyryl cyclic adenosine monophosphate (db-cAMP) in conventional ET-K to attenuate lung ischemia-reperfusion injury. In this study, the effi...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000008783906928
更新日期:2008-01-01 00:00:00
abstract::"Proof-of-principle" that cell replacement therapy works for neurodegeneration has been reported, but only using donor cells collected from fetal brain tissue obtained from surgical terminations of pregnancy. Surgical terminations of pregnancy represent an increasingly limited supply of donor cells due to the tendenc...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368910X546580
更新日期:2011-01-01 00:00:00
abstract::Bone marrow-derived cells have different plastic properties, especially regarding cell fusion, which increases with time and is prompted by tissue injury. Several recessive mutations, including Purkinje Cell Degeneration, affect the number of Purkinje cells in homozygosis; heterozygous young animals have an apparently...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368912X638900
更新日期:2012-01-01 00:00:00
abstract::Development of β-cells from human embryonic stem cells (hESCs) could compensate for the shortage of islet donors required for diabetes therapy. Although pancreatic progenitors have been derived from hESCs using various protocols, no fully functional b-cells could be generated in vitro. We evaluated the in vivo growth ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368912X636812
更新日期:2013-01-01 00:00:00