Abstract:
:The anatomical specificity of axon growth from fetal pig septal xenografts was studied by transplanting septal cells from E30-35 pig fetuses into cholinergic deafferented (192-IgG-saporin-infused) rats or into aged rats (> 18 months). Cell suspensions (100,000 cells/microl) were injected bilaterally into the dorsal and ventral hippocampus of immunosuppressed rats (10 mg/kg/day cyclosporine A). To assess axonal growth and synapse formation, acetylcholinesterase histochemistry, an antibody to choline acetyltransferase (ChAT), and three pig-positive/rat-negative antibodies: bovine 70kD neurofilament (NF70), human low-affinity NGF receptor (hNGFr), and human synaptobrevin (hSB) were used. In rats with surviving grafts at 6 months, NF70 axonal labeling was more extensive than either ChAT or hNGFr labeling. All three markers demonstrated graft axons extending selectively through the hippocampal CA fields and the molecular layer of the dentate gyrus. Graft axons did not extend into adjacent entorhinal cortex or neocortex. The distribution of pig hSB-positive synapses correlated with AChE-positive fiber outgrowth in to the host. Electron microscopic analysis of hSB-immunostained hippocampal sections revealed pig presynaptic terminals in contact with normal rat postsynaptic structures in the CA fields and the dentate gyrus. These data demonstrate target-appropriate growth of pig cholinergic axons and the formation of cross-species synapses in the deafferented or aged rat hippocampus.
journal_name
Cell Transplantjournal_title
Cell transplantationauthors
Deacon T,Whatley B,LeBlanc C,Lin L,Isacson Odoi
10.1177/096368979900800104subject
Has Abstractpub_date
1999-01-01 00:00:00pages
111-29issue
1eissn
0963-6897issn
1555-3892journal_volume
8pub_type
杂志文章abstract::The purpose of this study was to investigate the clinical efficacy of neural stem/progenitor cell (NS/PC) transplantation to treat severe cortical visual impairment (CVI), a sequela of neonatal brain injury. Fifty-two patients with cerebral injury and CVI were randomly divided into two groups: the treatment group (n =...
journal_title:Cell transplantation
pub_type: 杂志文章,随机对照试验
doi:10.3727/096368913X672163
更新日期:2013-01-01 00:00:00
abstract::Cell-based therapy for the bladder has its beginnings in the 1990s with the successful isolation and culture of bladder smooth muscle cells. Since then, several attempts have been made to artificially implant native cell types and stem cell-derived cells into damaged bladders in the form of single-cell injectables or ...
journal_title:Cell transplantation
pub_type: 杂志文章,评审
doi:10.3727/096368914X685050
更新日期:2015-01-01 00:00:00
abstract::Multipotent adult progenitor cells (MAPCs) are bone marrow-derived nonhematopoietic stem cells with a broad differentiation potential and extensive expansion capacity. A comparative study between human mesenchymal stem cells (hMSCs) and human MAPCs (hMAPCs) has shown that hMAPCs have clearly distinct phenotypical and ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368912X657369
更新日期:2013-01-01 00:00:00
abstract::Recent advances in islet transplantation using highly purified islets and effective immunosuppression strategies have resulted in substantial improvement in achieving insulin independence in type 1 diabetes patients. However, there are side effects from long-term immunosuppression, and transplant rejection and/or the ...
journal_title:Cell transplantation
pub_type: 杂志文章,评审
doi:10.3727/000000008783907107
更新日期:2008-01-01 00:00:00
abstract::Cell therapy has been shown to be a promising alternative therapy for neurorestoration, and more than 30 different types of cells have been shown to possess some capability to restore lost or damaged neurological functions. Chinese physicians have successfully applied cell therapy to many neurological diseases and dam...
journal_title:Cell transplantation
pub_type: 杂志文章,评审
doi:10.3727/096368913X672082
更新日期:2013-01-01 00:00:00
abstract::Granulocyte colony-stimulating factor (G-CSF) mobilizes peripheral blood stem cells (PBSCs) derived from bone marrow. We hypothesized that intraspinal transplantation of PBSCs mobilized by G-CSF could promote functional recovery after spinal cord injury. Spinal cords of adult nonobese diabetes/severe immunodeficiency ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368915X688146
更新日期:2016-01-01 00:00:00
abstract::Epithelial wound repair assures the recovery of the epithelial barrier after wounding. During wound healing epithelial cells migrate to cover the wound surface. The presented experiments were carried out to compare the migration of human keratinocytes from primary and secondary culture on polystyrene, collagen, and fi...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
abstract::Potential labels for identifying embryonic raphe neurons and a clonal, neuronally differentiating, raphe-derived cell line, RN33B, in CNS transplantation studies were tested by first characterizing the labels in vitro. The labels that were tested included 4',6-diamidino-2-phenylindole hydrochloride, 1,1'-dioctadecyl-3...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368979300200207
更新日期:1993-03-01 00:00:00
abstract:BACKGROUND:Extracellular vesicles (EVs) released from mesenchymal stem/stromal cells (MSCs) mediate their paracrine effect, but their efficacy to protect the microcirculation of the kidney is unknown. Using a novel swine model of unilateral renovascular disease (RVD) complicated by metabolic syndrome (MetS), we tested ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689718780942
更新日期:2018-07-01 00:00:00
abstract::We attempted to regenerate bone in a significant osseous defect with various stem cells from deciduous teeth, extracted from puppies, and grafted them into a parent canine mandible as an allograft, parent dental pulp, and bone marrow by tissue engineering and regenerative medicine technology using platelet-rich plasm...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368910X539128
更新日期:2011-01-01 00:00:00
abstract::International standards for stem cell treatment of neurological disorders have not yet been established. In particular, specific quantitative methods have not yet been adopted to assess the effectiveness of stem cell treatment. The aim of this study is to evaluate the functional changes detectable by conventional neu...
journal_title:Cell transplantation
pub_type: 临床试验,杂志文章
doi:10.3727/096368912x633761
更新日期:2012-01-01 00:00:00
abstract::Pancreatic islet transplantation is limited by shortage of donor organs. Although beta-cell lines could be used, their secretion of insulin is characteristically glucose independent and immunoisolation is required. Here we show that intrasplenic transplantation of encapsulated glucose-responsive mouse insulinoma cells...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000005783982990
更新日期:2005-01-01 00:00:00
abstract::In multiple sclerosis (MS), a chronic inflammatory relapsing demyelinating disease, failure to control or repair damage leads to progressive neurological dysfunction and neurodegeneration. Implantation of neural stem cells (NSCs) has been shown to promote repair and functional recovery in the acute experimental autoi...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368911X637443
更新日期:2012-01-01 00:00:00
abstract::An in vivo tracer technique that uses radiolabeled insulin as the tracer molecule has been developed to assess the rate of chemical transport between the cell transplantation chamber of an implantable bioartificial device and the host's circulatory system. The device considered here employs site-directed neovasculariz...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1016/0963-6897(95)90033-0
更新日期:1995-03-01 00:00:00
abstract::The periodontal ligament displays a reservoir of mesenchymal stem cells which can account for periodontal regeneration. Despite the numerous studies directed at the definition of optimal culture conditions for long-term expansion of periodontal ligament stem cells (PDLSCs), no consensus has been reached as to what is ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689718807680
更新日期:2019-01-01 00:00:00
abstract::A new extracorporeal bioartificial liver using alginate-entrapped hepatocytes was developed and evaluated for its ability to correct the lack of bilirubin conjugation in the Gunn rat. Hepatocytes were harvested from Sprague-Dawley rats by the two-step collagenase perfusion method and then immobilized in Ca(++)-alginat...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368979300200603
更新日期:1993-11-01 00:00:00
abstract::Transplantation of human cells after isolation and culture has become an important alternative for treatment of acute or chronic skin wounds. To increase the efficacy and reduce cost for transplantation of skin cells, more efficient and accurate techniques for evaluation of cell proliferation are needed. Hemocytometer...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000006783982115
更新日期:2006-01-01 00:00:00
abstract::Mesenchymal stem cells (MSCs) hold great promise for bone regeneration. However, the power of mesenchymal stem cells has not been applied to structural bone allografts in clinical practice. This study designed a new strategy to enhance the efficiency of allografts for segmental bone regeneration. Isolated MSCs were cu...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368909788809839
更新日期:2009-01-01 00:00:00
abstract::We investigated the effects of environmental enrichment (EE) on the function of transplanted adipose stem cells (ASCs) and the combined effect of EE and ASC transplantation on neurobehavioral function in an animal model of chronic hypoxic-ischemic (HI) brain injury. HI brain damage was induced in 7-day-old mice by uni...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368912X662390
更新日期:2013-01-01 00:00:00
abstract::Huntington's disease (HD) is a devastating neurodegenerative disorder that occurs in patients with a mutation in the huntingtin or IT15 gene. Patients are plagued by early cognitive signs, motor deficits, and psychiatric disturbances. Symptoms are attributed to cell death in the striatum and disruption of cortical-str...
journal_title:Cell transplantation
pub_type: 杂志文章,评审
doi:10.3727/000000007783464687
更新日期:2007-01-01 00:00:00
abstract::Isolation of islets from market-sized pigs is costly, with considerable islet losses from fragmentation occurring during isolation and tissue culture. Fetal and neonatal pigs yield insulin unresponsive islet-like cell clusters that become glucose-responsive after extended periods of time. Both issues impact clinical a...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368912X662372
更新日期:2014-03-01 00:00:00
abstract::The human neuronal cell-1 (HCN-1) line has recently been established. Under favorable conditions, these cells differentiate into mature neuronal phenotypes. Here we report on further characterization of these cells. Cultured HCN-1 cells express fibronectin immunoreactivity and grow well on fibronectin substrate but do...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368979200100104
更新日期:1992-01-01 00:00:00
abstract::Liver transplantation is the only existing modality for treating decompensated liver cirrhosis. Several factors, such as nonavailability of donors, combined with operative risks, complications associated with rejection, usage of immunosuppressive agents, and cost intensiveness, make this strategy available to only a f...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368910X498241
更新日期:2010-01-01 00:00:00
abstract::As a putative marker of cerebral small vessel disease, cerebral microbleeds (CMBs) have been associated with vascular cognitive impairment. Both iron accumulation and amyloid protein precursor (APP) dysregulation are recognized as pathological hallmarks underlying the progression of CMBs, but their cross-talk is not y...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689719831707
更新日期:2019-08-01 00:00:00
abstract::At present, there are still significant barriers that impede the clinical use of hESCs and iPS cells, including ethics, immunorejection, tumorigenesis from hESCs, and teratoma formation from iPS cells. It is therefore necessary to search for alternative sources of stem cells. WJ-MSCs originate from embryonic epiblasts...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368912X662444
更新日期:2013-01-01 00:00:00
abstract::Successful transplantation of cadaver embryonic neural tissue is highly dependent on the method used to obtain the tissue. It is important that the tissue not be contaminated bacteriologically by vaginal flora during the procedure, and that it not be disrupted mechanically. A low-pressure aspiration abortion technique...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368979400300202
更新日期:1994-03-01 00:00:00
abstract::The major limitation of nonhuman primate (NHP) embryonic stem (ES) cell research is inefficient genetic modification and limited knowledge of differentiation mechanisms. A genetically modified NHP-ES cell with biomarkers, such as green fluorescent protein (GFP), that allow noninvasive monitoring of transgenic cells, i...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000002783985350
更新日期:2002-01-01 00:00:00
abstract::In this study, we assessed the ability of miR-26b-5p to regulate T cell factor 4 (TCF-4) expression and thereby control human adipose-derived mesenchymal stem cell (hADMSC) adipogenic differentiation. Adipogenic medium was used to induce hADMSC differentiation over a 6-d period. The ability of miR-26b-5p to interact w...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689720934418
更新日期:2020-01-01 00:00:00
abstract::Persistent fetal vasculature (PFV) is a potentially serious developmental anomaly in human eyes, which results from a failure of the primary vitreous and the hyaloid vascular systems to regress during development. Recent findings from our laboratory indicate that fibrovascular membranes harvested from subjects with P...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368912X647153
更新日期:2012-01-01 00:00:00
abstract::Multilineage-differentiating stress-enduring (Muse) cells are endogenous pluripotent stem cells that can be isolated based on stage-specific embryonic antigen-3 (SSEA-3), a pluripotent stem cell-surface marker. However, their capacities for survival, neurotrophic factor secretion, and neuronal and glial differentiatio...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689719863809
更新日期:2019-09-01 00:00:00