Abstract:
:The clinical consequences of acute liver failure are associated with high mortality. Intensive medical intervention is required to treat the symptoms of liver failure, including coagulopathy, metabolic instability, and encephalopathy. Providing temporary liver support with an extracorporeal liver assist device could stabilize the patient until a donor liver became available or the patient's own liver was able to recover. The use of human hepatocytes as the biologic component of the assist device is precluded by the scarcity of available tissue and the limited proliferative potential of adult hepatocytes in vitro. Consequently, porcine hepatocytes are being evaluated as a cell source for liver assist devices. Maintaining differentiated function in isolated hepatocytes, however, remains a challenge in the development of this technology and is complicated by the fact that the key therapeutic functions for short-term survival have not been well defined. Several approaches have been effective in prolonging rodent hepatocyte function in vitro, including manipulation of extracellular matrix. Here, we have investigated porcine hepatocyte function in vitro with a specific emphasis on the response to exogenous collagen matrix. In control cultures, albumin secretion increased during the first 7-10 days of culture to an average of 50 +/- 17 microg/day/10(6) cells and then decreased over the next 2 weeks. The pattern of urea synthesis was slightly different in that it was highest in the first 1-3 days postisolation (140 +/- 19 microg/day/10(6) cells) and then decreased by about 50% to a plateau level that was stable during the next 3-4 weeks of culture. Cytochrome P450-mediated activities were the most labile with time in culture and were undetectable after the first week in the absence of pharmacological inducers. In contrast to results reported for rat cells, porcine hepatocytes exhibited differentiated function in the absence of any modification of the culture dish surface and function was not increased or prolonged in the presence of exogenous collagen.
journal_name
Cell Transplantjournal_title
Cell transplantationauthors
Gregory PG,Connolly CK,Toner M,Sullivan SJdoi
10.1177/096368970000900101subject
Has Abstractpub_date
2000-01-01 00:00:00pages
1-10issue
1eissn
0963-6897issn
1555-3892journal_volume
9pub_type
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journal_title:Cell transplantation
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journal_title:Cell transplantation
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journal_title:Cell transplantation
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doi:10.3727/096368916X692645
更新日期:2016-12-13 00:00:00
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更新日期:2007-01-01 00:00:00
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abstract::Circular RNAs (circRNAs) could sponge micro-RNAs (miRNAs) to regulate tumor progression of hepatocellular carcinoma (HCC). Hsa_circ_104566 contributes to papillary thyroid carcinoma progression. However, the tumorigenic mechanism of hsa_circ_104566 on HCC remains enigmatic. The role of hsa_circ_104566 on HCC was there...
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pub_type: 杂志文章,评审
doi:10.1177/0963689718773361
更新日期:2018-12-01 00:00:00
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journal_title:Cell transplantation
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doi:10.3727/096368908787236639
更新日期:2008-01-01 00:00:00
abstract::Addition of rIL-6 to IL-6-dependent B9 cells starved for IL-6 for 16-20 h stimulated a vigorous proliferative response. Glucocorticoids (GCs), in a concentration-dependent manner, inhibited rIL-6-stimulated proliferation of B9 cells This inhibition was specific for the GCs, evident by the capacity of the GCs, dexameth...
journal_title:Cell transplantation
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doi:
更新日期:2001-03-01 00:00:00
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abstract::The objective of this study was to define pretransplant islet culture conditions for optimum tissue engraftment in the rat islet allotransplantation model. Lewis rat islets were cultured in TCM 199/5% fetal calf serum for I day at 37 degrees C, followed by 1 day of culture at 22 degrees C. When islets from single dono...
journal_title:Cell transplantation
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doi:
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journal_title:Cell transplantation
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journal_title:Cell transplantation
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更新日期:2014-03-01 00:00:00
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journal_title:Cell transplantation
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journal_title:Cell transplantation
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