Abstract:
:Stem cells from dental tissues have been isolated and established for tooth regenerative applications. However, basic characterization on their biological properties still needs to be investigated before employing them for effective clinical trials. In this study, we compared the telomere length, relative telomerase activity (RTA), and relative reverse transcriptase activity (RRA) as well as the surface antigen profile and mesenchymal differentiation ability in human dental papilla stem cells (DPaSCs), dental pulp stem cells (DPuSCs), and dental follicle stem cells (DFSCs) with mesenchymal stem cells (MSCs) derived from bone marrow. Dental stem cells (DSCs) were strongly positive for cell surface markers, such as CD44 and CD90. However, slightly lower expression of CD105 was observed in DPaSCs and DPuSCs compared to DFSCs and MSCs. Following specific induction, DPaSCs, DFSCs, and MSCs were successfully differentiated into adipocytes and osteocytes. However, DPuSCS, in particular, were able to differentiate into adipocytes but failed to induce into osteogenic differentiation. Further, all DSCs, MSCs, and MRC-5 fibroblasts as control were investigated for telomere length by nonradioactive chemiluminescent assay, RTA by relative-quantitative telomerase repeat amplification protocol (RQ-TRAP), and RRA by PCR-based assay. Mean telomere lengths in DPaSCs, DPuSCs, DFSCs, and MSCs was ∼11 kb, and the values did not differ significantly (p < 0.05) among the cells analyzed. RTA levels in DPaSCs were significantly (p < 0.05) higher than in MSCs, DPuSCs, DFSCs, and MRC-5 fibroblasts and among DSCs, DFSCs showed a significantly (p < 0.05) lower RTA. Moreover, RRA levels were significantly (p < 0.05) higher in DPaSCs, DPuSCs, and MSCs than in DFSCs. Based on these observations, we conclude that among DSCs, DPaSCs possessed ideal characteristics on telomere length, telomerase activity and reverse transcriptase (RTase) activity, and may serve as suitable alternative candidates for regenerative medicine.
journal_name
Cell Transplantjournal_title
Cell transplantationauthors
Jeon BG,Kang EJ,Kumar BM,Maeng GH,Ock SA,Kwack DO,Park BW,Rho GJdoi
10.3727/096368911X565001subject
Has Abstractpub_date
2011-01-01 00:00:00pages
1693-705issue
11-12eissn
0963-6897issn
1555-3892pii
ct0279jeonjournal_volume
20pub_type
杂志文章abstract::Hepatocyte transplantation (HT) has become an effective therapy for patients with metabolic inborn errors. We report the clinical outcome of four children with metabolic inborn errors that underwent HT, describing the cell infusion protocol and the metabolic outcome of transplanted patients. Cryopreserved hepatocytes...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368912X637505
更新日期:2012-01-01 00:00:00
abstract::The aim of this work was to determine the effect of nicotine desensitization on dopamine (DA) release in the dorsal striatum and shell of the nucleus accumbens (NAc) from brain slices. In vitro fast-scan cyclic voltammetry analysis was used to evaluate dopamine release in the dorsal striatum and the NAc shell of Sprag...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689718775382
更新日期:2019-03-01 00:00:00
abstract::Superparamagnetic iron oxide nanoparticles (MNPs) together with magnetic resonance imaging (MRI) are the preferred tools for monitoring the fate and biodistribution of administered cells in stem cell therapy studies. Commercial MNPs need transfection agents and long incubation times for sufficient cell labeling and fu...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368916X691303
更新日期:2016-10-01 00:00:00
abstract::Potential labels for identifying embryonic raphe neurons and a clonal, neuronally differentiating, raphe-derived cell line, RN33B, in CNS transplantation studies were tested by first characterizing the labels in vitro. The labels that were tested included 4',6-diamidino-2-phenylindole hydrochloride, 1,1'-dioctadecyl-3...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368979300200207
更新日期:1993-03-01 00:00:00
abstract::There is conflicting evidence favoring both the use of human serum (HS) and of human serum albumin (HSA) in human islet culture. We evaluated the effects of HS versus HSA supplementation on 1) in vitro β-cell viability and function and 2) in vivo islet graft revascularization, islet viability, β-cell death, and metabo...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368915X688119
更新日期:2016-01-01 00:00:00
abstract::Traumatic brain injury (TBI), a major contributor to deaths and permanent disability worldwide, has been recently described as a progressive cell death process rather than an acute event. TBI pathophysiology is complicated and can be distinguished by the initial primary injury and the subsequent secondary injury that ...
journal_title:Cell transplantation
pub_type: 杂志文章,评审
doi:10.3727/096368915X686913
更新日期:2015-01-01 00:00:00
abstract::Posttransplant infusion of viable donor bone marrow cells (DBMC) has been shown in our previous studies to promote acceptance of incompatible kidney allografts in rhesus monkeys after treatment with polyclonal antithymocyte globulin to deplete peripheral T-lymphocytes. In this nonhuman primate model, the infusion of t...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1016/s0963-6897(97)00005-5
更新日期:1997-07-01 00:00:00
abstract::Ischemia-reperfusion and immunosuppressive therapy are a major cause of progressive renal failure after kidney transplantation. Recent studies have shown that administration of bone marrow mesenchymal stem cells (MSCs) improves kidney functional recovery in the acute phase of post ischemia-reperfusion injury. In the ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368912X640448
更新日期:2012-01-01 00:00:00
abstract::Tissue damage by ischemia/reperfusion (I/R) results from a temporary cessation of blood flow followed by the restoration of circulation. The injury depresses mitochondrial respiration, increases the production of reactive oxygen species (ROS), decreases the mitochondrial transmembrane potential, and stimulates invasio...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368912X659862
更新日期:2014-02-01 00:00:00
abstract::Currently, different techniques to expand human hepatocytes in vitro are being investigated to generate enough cells for liver-directed cell therapies. However, based on observations in fibroblasts and other cell types, telomere attrition limits the proliferative capacity of normal somatic cells. Therefore, we explore...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000003771000138
更新日期:2003-01-01 00:00:00
abstract::Adipose tissue contains a mesenchymal stem cell (MSC) population known as adipose-derived stem cells (ASCs) capable of differentiating into different cell types. Our aim was to induce hepatic transdifferentiation of ASCs by sequential exposure to several combinations of cytokines, growth factors, and hormones. The mos...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368909X12483162197321
更新日期:2009-01-01 00:00:00
abstract::To examine the effects of autologous sural nerve and adrenal medullary tissue intrastriatal cografts upon voluntary motor performance in parkinsonism, a non-human primate 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) model was employed to quantitatively assess skilled hand movements. Motor performance was studie...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1016/0963-6897(95)92155-r
更新日期:1995-01-01 00:00:00
abstract::The transplantation of encapsulated dopamine-secreting cells into the striatum represents one potential means of treating Parkinson's disease. The present study investigated the ability of encapsulated PC12 cells, which are derived from rat pheochromocytoma, to supply L-dopa and dopamine into the primate brain in the ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368970000900519
更新日期:2000-09-01 00:00:00
abstract::Stroke is one of the most devastating diseases worldwide. In recent years, a great number of studies have focused on the effects of microRNAs (miRNAs) on stroke and the results demonstrated that the expressions of miRNAs are associated with the prognosis of stroke. In the present study, we review relevant articles reg...
journal_title:Cell transplantation
pub_type: 杂志文章,评审
doi:10.1177/0963689718773361
更新日期:2018-12-01 00:00:00
abstract::Cell therapies consist in transplanting healthy cells into a disabled tissue with the goal to repopulate it and restore its function at least partially. In muscular diseases, most of the time, myoblasts are chosen for their expansion capacity in culture. Nevertheless, cell transplantation has limitations, among them,...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368912X655028
更新日期:2012-01-01 00:00:00
abstract::Huntington's disease (HD) produces severe neurodegeneration in the striatum leading to disabling motor impairments, including the loss of control of skilled reaching movements. Fetal GABAergic transplants can physically replace the lost striatal cells but with only partial success in functional recovery. Here, we aime...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368912X657918
更新日期:2013-01-01 00:00:00
abstract::Recently, we described a diffusion chamber for a bioartificial endocrine pancreas (Bio-AEP). Pancreatic islet cells in the Bio-AEP device were isolated from the immune system of the host by an artificial barrier, while nutrients, electrolytes, oxygen, and bioactive secretory products were exchanged across this barrier...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368979900800409
更新日期:1999-07-01 00:00:00
abstract::Ensheathing glia have been demonstrated to have neuroregenerative properties but this cell type from human sources has not been extensively studied because tissue samples are not easily obtained, primary cultures are slow growing, and human cell lines are not available. We previously isolated immortalized ensheathing ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368910X522108
更新日期:2011-01-01 00:00:00
abstract::Techniques for medical tissue regeneration require an abundant source of human adult stem cells. There is increasing evidence that adipose stem cells contribute to restoration of tissue vascularization and organ function. The object of our study was to isolate and characterize adult adipose-derived stem cells from pat...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/09638910X519265
更新日期:2010-01-01 00:00:00
abstract::This study was designed to investigate functional recovery after the transplantation of mesenchymal stem cells (MSCs) or neurally differentiated MSCs (NMSCs) derived from bone marrow in a rat model of spinal cord injury (SCI). Sprague-Dawley rats were subjected to incomplete SCI using an NYU impactor to create a free ...
journal_title:Cell transplantation
pub_type: 杂志文章,已发布勘误
doi:10.3727/096368916X692078
更新日期:2016-07-01 00:00:00
abstract::Researchers have accumulated a decade of experience with autologous cell therapy in the treatment of critical limb ischemia (CLI). We conducted a systematic review of clinical trials in the literature to determine the safety and efficacy of cell therapy in CLI. We searched the literature for clinical trials of autolog...
journal_title:Cell transplantation
pub_type: 杂志文章,meta分析,评审
doi:10.3727/096368912X636777
更新日期:2013-01-01 00:00:00
abstract::In cartilage tissue engineering using stem cells, it is important to stimulate proliferation and control the differentiation of stem cells to specific lineages. Here we reported a combined technique for articular cartilage repair, consisting of bone marrow mesenchymal stem cells (BMMSCs) transfected with connective ti...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368913X669770
更新日期:2014-04-01 00:00:00
abstract::Neural progenitor cells (NPCs) overexpressing fibroblast growth factor 2 (FGF-2) have the distinct tendency to associate with the vasculature and establish multiple proliferative clusters in the perivascular environment after transplantation into the cerebral cortex. Strikingly, the vascular clusters of progenitor cel...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368916X690421
更新日期:2016-01-01 00:00:00
abstract::Human marrow stromal cells (hMSCs) provide functional benefit in rats subjected to stroke. Astrocytes are coupled into a cellular network via gap junction channels, predominantly composed of connexin-43 (Cx43) proteins. Astrocytes are believed to play a vital role in neuroprotection by providing energy substrates to n...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000005783983205
更新日期:2005-01-01 00:00:00
abstract::The choice of enzyme blend is critical for successful islet isolation. Islet yield, viability, integrity, and function are important factors that influence the outcome of islet transplantation. Liberase HI has been used as a standard enzyme for pancreas digestion and has successfully produced islets that reversed dia...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368911X623826
更新日期:2012-01-01 00:00:00
abstract::Natural immunological tolerance can be induced in certain types of allogeneic liver transplantation in rats. To screen for genes associated with the induction of tolerance, suppression subtractive hybridization was performed in the rat liver transplantation model between a DA donor and PVG recipient combination where ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000008783906955
更新日期:2008-01-01 00:00:00
abstract::Sepsis is a life-threatening disease that results in excessive stimulation of the host's immune cells. In the animal study, the purpose was to investigate the roles of fresh frozen plasma (FFP) transfusion in shaping the CD4+ T lymphocytes immune response through modulating the secreted exosome protein Galectin-9 in m...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689720947347
更新日期:2020-01-01 00:00:00
abstract::Parkinson's disease (PD), while primarily associated with degeneration of nigrostriatal dopamine neurons, is now increasingly recognized to have more widespread cell loss and so the most effective cell replacement therapy should target all these neuronal losses. Neural precursor cells might be ideal in this regard as ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000006783981684
更新日期:2006-01-01 00:00:00
abstract::Several molecular therapies require the implantation of cells that secrete biotherapeutic molecules and imaging the location and microenvironment of the cellular implant to ascertain its function. We demonstrate noninvasive in vivo magnetic resonance imaging (MRI) of self-assembled microcontainers that are capable of ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000007783464803
更新日期:2007-01-01 00:00:00
abstract::The clinical consequences of acute liver failure are associated with high mortality. Intensive medical intervention is required to treat the symptoms of liver failure, including coagulopathy, metabolic instability, and encephalopathy. Providing temporary liver support with an extracorporeal liver assist device could s...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368970000900101
更新日期:2000-01-01 00:00:00