Abstract:
:Somatic stem cells can be isolated from a variety of sources. Although some studies have suggested that somatic stem cells may represent a cell population that is very similar to embryonic stem (ES) cells, it remains unclear whether somatic stem cells retain the potential to differentiate into any cell type derived from the three germ layers. In this study, we investigated the transdifferentiation potential of somatic stem cells using adipose tissue-derived stem/progenitor cells (ASCs; mesodermal stem cells) and pancreatic stem cells (endodermal stem cells). Previous reports from other groups describe the protocol that has been used to differentiate ASCs or mesenchymal stem cells (MSCs) in bone marrow into insulin-producing cells. Induction 1: ASCs were cultured for 3 days in ultra-low attachment plates under serum-free conditions. Induction 2: ASCs were cultured for 24 h with L-DMEM, and reinduced with serum-free H-DMEM for another 10 h. Unlike previous reports, we did not get ASCs to express any pancreas-specific genes, including insulin-1 or insulin-2. Pancreatic stem cells were induced to differentiate into adipo/osteogenic by the following protocols. Induction protocol 1: ACSs were cultured for 7 days with medium containing indometacin, dexamethasone, hydrocortisone, and insulin for adipogenic differentiation. Induction protocol 2: The cells were cultured for 7 days with medium containing dexamethasone, ascorbate-2-phosphate, and beta-glycerophosphate for osteogenic differentiation. Although these approaches have been widely used for adipo/osteogenic differentiation from MSCs, adipo/osteogenic differentiation from pancreatic stem cells was not observed. These data suggest that it is not easy for somatic stem cells to transdifferentiate into other germ cell types, at least, under these conditions.
journal_name
Cell Transplantjournal_title
Cell transplantationauthors
Oishi K,Noguchi H,Yukawa H,Hayashi Sdoi
10.1177/096368970901805-614subject
Has Abstractpub_date
2009-01-01 00:00:00pages
581-9issue
5eissn
0963-6897issn
1555-3892journal_volume
18pub_type
杂志文章abstract::Mouse embryonal carcinoma cell line P19 could be differentiated to neurons or glial cells by appropriate induction. We introduced the lacZ gene driven by a calmodulin promoter into P19 cells, and cloned the transfectant CaM-Z that showed the strong expression of lacZ gene after the induction of differentiation. CaM-Z ...
journal_title:Cell transplantation
pub_type: 杂志文章
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journal_title:Cell transplantation
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doi:10.3727/096368909788809839
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journal_title:Cell transplantation
pub_type: 杂志文章
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pub_type: 杂志文章,评审
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更新日期:2018-05-01 00:00:00
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journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368913X663550
更新日期:2014-01-01 00:00:00
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journal_title:Cell transplantation
pub_type: 杂志文章
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journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368912X654948
更新日期:2013-01-01 00:00:00
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journal_title:Cell transplantation
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journal_title:Cell transplantation
pub_type: 杂志文章,评审
doi:10.1016/0963-6897(95)00035-v
更新日期:1995-09-01 00:00:00
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doi:10.1177/0963689719864318
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journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368908784153850
更新日期:2008-01-01 00:00:00
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journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000005783982783
更新日期:2005-01-01 00:00:00
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journal_title:Cell transplantation
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doi:10.1177/0963689719857085
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journal_title:Cell transplantation
pub_type: 临床试验,杂志文章,随机对照试验
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journal_title:Cell transplantation
pub_type: 杂志文章
doi:
更新日期:2008-01-01 00:00:00
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journal_title:Cell transplantation
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pub_type: 杂志文章,随机对照试验
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更新日期:2014-01-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2017-09-01 00:00:00
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journal_title:Cell transplantation
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