Abstract:
:We developed a reliable and quantitative method for measuring the dynamic process of unidirectional two-dimensional (2-D) tissue formation of endothelial cells (ECs) in vitro. The culturing of bovine ECs in an assembled culture chamber provided a square monolayered cell sheet with a linear margin when disassembled at the confluency. The cell sheet maintained in culture showed a unidirectional which was determined from the daily observation of tissue, allowed us to determine quantitatively the dynamic process of unidirectional endothelialization in vitro. The endothelialized distance and the endothelializing zone on a glass slide were found to be nearly 500 microns/day and 750 microns in width, respectively. Thus, the method developed here provided information of the 2-D tissue formation process. This model would be useful as an in vitro model which simulates the anastomotic endothelialization of an artificial vascular graft.
journal_name
Cell Transplantjournal_title
Cell transplantationauthors
Niu S,Matsuda Tdoi
10.1177/096368979200100601subject
Has Abstractpub_date
1992-01-01 00:00:00pages
375-81issue
6eissn
0963-6897issn
1555-3892journal_volume
1pub_type
杂志文章abstract::Stem cells are unique cells in the ability that can self-renew and differentiate into a wide variety of cell types, suggesting that a specific molecular control network underlies these features. To date, stem cells have been applied to many clinical therapeutic approaches. For example, hematopoietic stem cells (HSCs) ...
journal_title:Cell transplantation
pub_type: 杂志文章,评审
doi:10.3727/096368909X471224
更新日期:2009-01-01 00:00:00
abstract::Schwann cells naturally support axonal regeneration after injury in the peripheral nervous system, and have also shown a significant, albeit limited, ability to support axonal growth and remyelination after grafting to the central nervous system (CNS). It is possible that Schwann cell-induced axonal growth in the CNS ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1016/s0963-6897(97)00166-8
更新日期:1998-03-01 00:00:00
abstract::End-stage organ failure is a major cause of death worldwide that can occur in patients of all ages and transplantation is the current standard of care for chronic end-stage disease of many organs. Despite the success of organ transplantation, it is becoming clear that there will never be enough organs made available t...
journal_title:Cell transplantation
pub_type: 历史文章,杂志文章
doi:10.3727/000000006783982340
更新日期:2006-01-01 00:00:00
abstract::Adrenal medullary tissue including chromaffin cells was grafted intrathecally in cancer patients to relieve intractable pain. The central nervous system (CNS) is considered an immune privileged site. Therefore, non-HLA-matched and unencapsulated tissue was grafted in 15 patients and 1 sham control in a series of at le...
journal_title:Cell transplantation
pub_type: 临床试验,杂志文章
doi:10.1177/096368970000900111
更新日期:2000-01-01 00:00:00
abstract::Radiotherapy is an effective treatment for brain tumors but often results in cognitive deficits in survivors. Transplantation of embryonic or brain-derived neural stem/progenitor cells (BNSPCs) ameliorated cognitive impairment after irradiation (IR) in animal models. However, such an approach in patients requires a cl...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368913X674648
更新日期:2014-01-01 00:00:00
abstract::There has been no gold standard for intraoperative monitoring in carotid endarterectomy (CEA) till now. The purpose of the current study was to investigate the value of near-infrared spectroscopy (NIRS) monitoring in CEA and explore the thresholds for intraoperative cerebral hypoperfusion. Eighty-four consecutive pati...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689718817760
更新日期:2019-02-01 00:00:00
abstract::A fresh osteochondral allograft is one of the most effective treatments for cartilage defects of the knee. Despite the clinical success, fresh osteochondral allografts have great limitations in relation to the short storage time that cartilage tissues can be well-preserved. Fresh osteochondral grafts are generally sto...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368970901805-604
更新日期:2009-01-01 00:00:00
abstract::Bone marrow (BM) cells may interact with coronary endothelium and modulate coronary atherosclerosis. We investigated the time course of coronary luminal loss and changes in conductance after intracoronary injection of enriched hematopoietic BM stem cells in patients with previous myocardial infarction (MI). Among 24 p...
journal_title:Cell transplantation
pub_type: 杂志文章,随机对照试验
doi:10.3727/096368907783338244
更新日期:2007-01-01 00:00:00
abstract::Persistent corneal wounds result from numerous eye disorders, and to date, available treatments often fail to accelerate reepithelialization, the key initial step in wound healing. To speed reepithelialization, we explored a cell-transfer transplant method utilizing polydimethylsiloxane (PDMS) contact lenses to delive...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000005783982783
更新日期:2005-01-01 00:00:00
abstract::Embedded-culture of pancreatic beta-cells derived from a transgenic mouse insulinoma (MIN6 cells) was studied in vitro and in vivo. The MIN6 cells were enmeshed in an agarose-PVMA-collagen matrix for long-term maintenance. The cells formed islet-like cell clusters (ICCs) in the mixed matrix. When 10 mmol/L nicotinamid...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1016/0963-6897(95)00001-e
更新日期:1995-05-01 00:00:00
abstract::One of the serious obstacles of the aortopathies research is a considerable shortage of human aortic smooth muscle cells (SMCs), which can be used to model the disease. SMC in most cases come from the whole aorta of transplant donors, which are rather difficult to access. In the course of coronary artery bypass graft ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689717721226
更新日期:2017-10-01 00:00:00
abstract::The diverse requirements for a successful islet encapsulation barrier suggest the benefit of a barrier system that presents differing functionalities to encapsulated cells and host cells. Initially, multifunctional hydrogels were synthesized via the sequential photopolymerization of PEG hydrogel layers, each with diff...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000007783472336
更新日期:2007-11-01 00:00:00
abstract::Several molecular therapies require the implantation of cells that secrete biotherapeutic molecules and imaging the location and microenvironment of the cellular implant to ascertain its function. We demonstrate noninvasive in vivo magnetic resonance imaging (MRI) of self-assembled microcontainers that are capable of ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000007783464803
更新日期:2007-01-01 00:00:00
abstract::Despite the substantial efforts to elucidate the role of early brain injury in subarachnoid hemorrhage (SAH), an effective pharmaceutical therapy for patients with SAH continues to be unavailable. This study aims to reveal the role of necroptosis after SAH, and explore whether the disruption of the blood-brain barrier...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689719867285
更新日期:2019-11-01 00:00:00
abstract::Tumor recurrence is the main limitation of liver transplantation (LT) in patients with hepatocellular carcinoma (HCC) and can be promoted by immunosuppressants. However, there is no prevention or treatment for HCC recurrence after LT. Here we describe a clinical-scale method for an adoptive immunotherapy approach that...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368911X627589
更新日期:2012-01-01 00:00:00
abstract::Hepatocyte transplantation is a promising therapy for acute liver failure in humans. Recently, we succeeded in inducing various acute and chronic liver failures in nude mice. Engraftment of transplanted xenogeneic rat hepatocytes, visualized in the host liver by anti-MHC class I immunohistochemistry, revealed that liv...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368908785096051
更新日期:2008-01-01 00:00:00
abstract::Current good manufacturing practice (cGMP) islet processing facilities provide an ultraclean environment for the safe production of clinical grade islets for transplantation into immunosuppressed diabetic recipients. The objective of this study was to monitor the rate of microbial contamination in islet products after...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:
更新日期:2007-01-01 00:00:00
abstract::Porcine islets show notoriously low insulin secretion levels in response to glucose stimulation. While this is somehow expected in the case of immature islets isolated from fetal and neonatal pigs, disappointingly low secretory responses are frequently reported in studies using in vitro-maturated fetal and neonatal is...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368916X693798
更新日期:2017-05-09 00:00:00
abstract::ET-Kyoto solution (ET-K) is an extracellular-type organ preservation solution containing the cytoprotective disaccharide, trehalose. A previous study reported the supplement of dibutyryl cyclic adenosine monophosphate (db-cAMP) in conventional ET-K to attenuate lung ischemia-reperfusion injury. In this study, the effi...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000008783906928
更新日期:2008-01-01 00:00:00
abstract::This study examined how the difference in pore size of porous scaffolds affected the in vitro chondrogenic differentiation of seeded adipose stem cells (ASCs) and the in vivo cartilage repair of ASC/scaffold construct. ASCs were isolated from 18 rabbits and seeded in a porous poly (ε-caprolactone) (PCL) scaffold with ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368912X638865
更新日期:2012-01-01 00:00:00
abstract::Human skin contains keratinocytes in the epidermis. Such cells share their ectodermal origin with the central nervous system (CNS). Recent studies have demonstrated that terminally differentiated somatic cells can adopt a pluripotent state, or can directly convert its phenotype to neurons, after ectopic expression of ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689720978219
更新日期:2021-01-01 00:00:00
abstract::Successful repair of damage in cerebral palsy (CP) needs effective clinical interventions other than simply symptomatic treatments. To elucidate the feasibility of using olfactory ensheathing cells (OECs) to treat CP in children and adolescents, we conducted a randomized controlled clinical trial (RCT) on 33 patients....
journal_title:Cell transplantation
pub_type: 杂志文章,随机对照试验
doi:10.3727/096368910X492652
更新日期:2010-01-01 00:00:00
abstract::Multiple sclerosis (MS) is a severe debilitating disorder characterized by progressive demyelination and axonal damage of the central nervous system (CNS). Current therapies for MS inhibit the immune response and demonstrate reasonable benefits if applied during the early phase of relapsing–remitting MS (RRMS) while t...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368912X657404
更新日期:2013-01-01 00:00:00
abstract::The transplantation of porcine islets of Langerhans to treat type 1 diabetes may provide a solution to the demand for insulin-producing cells. Porcine islets encapsulated in agarose-agarose macrobeads have been shown to function in nonimmunosuppressed xenogeneic models of both streptozotocin-induced and autoimmune typ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368913X667033
更新日期:2014-01-01 00:00:00
abstract::The need for chronic immune suppression (IS) is one of the hurdles precluding widespread use of islet cell transplantation to restore glycemic control in patients with type 1 diabetes. We report the case of a healthy nonhuman primate (NHP) treated on and off for over 2.5 years with steroid-free IS, consisting of dacli...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689718823505
更新日期:2019-03-01 00:00:00
abstract::Researchers have accumulated a decade of experience with autologous cell therapy in the treatment of critical limb ischemia (CLI). We conducted a systematic review of clinical trials in the literature to determine the safety and efficacy of cell therapy in CLI. We searched the literature for clinical trials of autolog...
journal_title:Cell transplantation
pub_type: 杂志文章,meta分析,评审
doi:10.3727/096368912X636777
更新日期:2013-01-01 00:00:00
abstract::This study aimed to explore the characteristics of TGFBR1-epidermal growth factor receptor (EGFR)-CTNNB1-CDH1 axis in regulating the invasion and migration in lung cancer. Using the small interfering RNA technology, EGFR was silenced in H2170 and H1299 cells. Then, the colony formation, migration, and invasion abiliti...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689720969167
更新日期:2020-01-01 00:00:00
abstract::Adipose tissue contains a mesenchymal stem cell (MSC) population known as adipose-derived stem cells (ASCs) capable of differentiating into different cell types. Our aim was to induce hepatic transdifferentiation of ASCs by sequential exposure to several combinations of cytokines, growth factors, and hormones. The mos...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368909X12483162197321
更新日期:2009-01-01 00:00:00
abstract::The limited availability of human embryonic tissue for dopamine cell transplants in Parkinson's patients has led to an increased interest in using xenogeneic donor tissue. Unfortunately, without aggressive immunosuppression, such brain xenografts are rejected by the host immune system. Chronic brain xenograft rejectio...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
abstract::The main purpose of this work has been to establish a new culturing technique to improve the chondrogenic commitment of isolated adult human chondrocytes, with the aim of being used during cell-based therapies or tissue engineering strategies. By using a rather novel technique to generate scaffold-free three-dimension...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368908786576499
更新日期:2008-01-01 00:00:00