Abstract:
:This study aimed to explore the characteristics of TGFBR1-epidermal growth factor receptor (EGFR)-CTNNB1-CDH1 axis in regulating the invasion and migration in lung cancer. Using the small interfering RNA technology, EGFR was silenced in H2170 and H1299 cells. Then, the colony formation, migration, and invasion abilities were detected using colony-forming assay and transwell assay. Moreover, the mRNA expression of smad2, smad3, CTNNB1, and CDH1, and the protein expression of TGFBR1, CDH1, and TCF were determined using the real-time polymerase chain reaction and western blotting. The results showed that silencing EGFR could significantly decrease the colony-forming ability in H2170 and H1299. Knocking down EGFR could significantly inhibit the invasion and migration ability of H2179 and H1299. Inhibiting the expression of EGFR could significantly decrease the expression of smad2, smad3, CDH1, and CTNNB1, with all P-values <0.05. In addition, silencing EGFR could markedly decrease the expression of TGFBR1 and CDH1 in H1299 and H2170, with all P-values <0.05. In conclusion, silencing EGFR could significantly regulate the progression of lung cancer via TGFBR1-EGFR-CTNNB1-CDH1 axis in Wnt signaling pathway.
journal_name
Cell Transplantjournal_title
Cell transplantationauthors
Liu R,Zhang Y,Ding Y,Zhang S,Pan Ldoi
10.1177/0963689720969167subject
Has Abstractpub_date
2020-01-01 00:00:00pages
963689720969167eissn
0963-6897issn
1555-3892journal_volume
29pub_type
杂志文章abstract::During the past 15 years, a variety of peptides, known as protein transduction domains (PTDs), or cell-penetrating peptides (CPPs), have been characterized for their ability to translocate into live cells. There are now numerous examples of biologically active full-length proteins and peptides that have been successfu...
journal_title:Cell transplantation
pub_type: 杂志文章,评审
doi:10.3727/096368910X508744
更新日期:2010-01-01 00:00:00
abstract::Autologous brain cell transplantation might be useful for repairing lesions and restoring function of the central nervous system. We have demonstrated that adult monkey brain cells, obtained from cortical biopsy and kept in culture for a few weeks, exhibit neural progenitor characteristics that make them useful for br...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368909X470847
更新日期:2009-01-01 00:00:00
abstract::Human islets are subjected to a number of stresses before and during their isolation that may influence their survival and engraftment after transplantation. Apoptosis is likely to be activated in response to these stresses. Apoptosis due to intrinsic stresses is regulated by pro- and antiapoptotic members of the Bcl...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368911X566262
更新日期:2012-01-01 00:00:00
abstract::Prior studies have suggested the possibility of immune-mediated death of xenogeneic hepatocytes in a bioartificial liver (BAL) during hemoperfusion. This study was designed to elucidate how immunity may cause death of xenogeneic hepatocytes in the BAL. Healthy dogs were treated with a BAL containing hollow fiber membr...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000004783983378
更新日期:2004-01-01 00:00:00
abstract::The diverse requirements for a successful islet encapsulation barrier suggest the benefit of a barrier system that presents differing functionalities to encapsulated cells and host cells. Initially, multifunctional hydrogels were synthesized via the sequential photopolymerization of PEG hydrogel layers, each with diff...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000007783472336
更新日期:2007-11-01 00:00:00
abstract::In previous work, we engineered functional cell sheets using bone marrow-derived mesenchymal stem cells (BM-MSCs) to promote islet graft survival. In the present study, we hypothesized that a cell sheet using dermal fibroblasts could be an alternative to MSCs, and then we aimed to evaluate the effects of this cell she...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368916X690854
更新日期:2016-01-01 00:00:00
abstract::The present study was designed to evaluate the clinical outcome of cell-based therapy with cultured adipose derived stromal cells (ASCs) for the treatment of cutaneous manifestations in patients affected by systemic sclerosis (SSc). ASCs have an extraordinary developmental plasticity, including the ability to undergo ...
journal_title:Cell transplantation
pub_type: 临床试验,杂志文章
doi:10.3727/096368912X639017
更新日期:2013-01-01 00:00:00
abstract::Near infrared fluorescence (NIRF) optical imaging is a technique particularly powerful when studying in vivo processes at the molecular level in preclinical animal models. We recently demonstrated liver irradiation under the additional stimulus of partial hepatectomy as being an effective primer in the rat liver repop...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:
更新日期:2009-01-01 00:00:00
abstract::Recent advances in tissue engineering technologies have highlighted the ability to create functional liver systems using isolated hepatocytes in vivo. Considering the serious shortage of donor livers that can be used for hepatocyte isolation, it has remained imperative to establish a hepatocyte propagation protocol t...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368911X605330
更新日期:2012-01-01 00:00:00
abstract::In previous studies we demonstrated that transplantation of microencapsulated hepatocytes could correct congenital hyperbilirubinemia in Gunn rats for 4 to 6 wks. Reduction in hyperbilirubinemia followed a single transplantation of isolated encapsulated hepatocytes (IEH). After 4 to 6 wks of transplantation IEH gradua...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368979200100404
更新日期:1992-01-01 00:00:00
abstract::Bisphosphonates (BPs) are widely used to treat several metabolic and oncological diseases affecting the skeletal system. Despite BPs' well-known therapeutic potential, they also displayed important side effects, among which is BPs-related osteonecrosis of the jaw, by targeting osteoclast activities, osteoblast, and os...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689720948497
更新日期:2020-01-01 00:00:00
abstract::ET-Kyoto solution (ET-K) is an extracellular-type organ preservation solution containing the cytoprotective disaccharide, trehalose. A previous study reported the supplement of dibutyryl cyclic adenosine monophosphate (db-cAMP) in conventional ET-K to attenuate lung ischemia-reperfusion injury. In this study, the effi...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000008783906928
更新日期:2008-01-01 00:00:00
abstract::Posttransplant infusion of viable donor bone marrow cells (DBMC) has been shown in our previous studies to promote acceptance of incompatible kidney allografts in rhesus monkeys after treatment with polyclonal antithymocyte globulin to deplete peripheral T-lymphocytes. In this nonhuman primate model, the infusion of t...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1016/s0963-6897(97)00005-5
更新日期:1997-07-01 00:00:00
abstract::Recently, animal studies have demonstrated the efficacy of endothelial progenitor cell (EPC) therapy for diabetic wound healing. Based on these preclinical studies, we performed a prospective clinical trial phase I/IIa study of autologous G-CSF-mobilized peripheral blood (PB) CD34(+) cell transplantation for nonhealin...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368912X658007
更新日期:2014-02-01 00:00:00
abstract::Autologous cardiosphere-derived cells (CDCs) were the first therapeutic modality to demonstrate myocardial regeneration with a decrease in scar size and an increase in viable, functional tissue. Widespread applicability of autologous CDC therapy is limited by the need for patient-specific myocardial biopsy, cell proce...
journal_title:Cell transplantation
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.3727/096368916X692933
更新日期:2017-02-16 00:00:00
abstract::Hepatocyte transplantation (HT) has become an effective therapy for patients with metabolic inborn errors. We report the clinical outcome of four children with metabolic inborn errors that underwent HT, describing the cell infusion protocol and the metabolic outcome of transplanted patients. Cryopreserved hepatocytes...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368912X637505
更新日期:2012-01-01 00:00:00
abstract::Hematopoietic stem and progenitor cells reside within the bone marrow (BM) microenvironment. By a well-balanced interplay between self-renewal and differentiation, they ensure a lifelong supply of mature blood cells. Physiologically, multiple different cell types contribute to the regulation of stem and progenitor cel...
journal_title:Cell transplantation
pub_type: 杂志文章,评审
doi:10.1177/0963689717745890
更新日期:2018-05-01 00:00:00
abstract::Adequate regulation of glucose levels by a microencapsulated pancreatic islet graft requires a minute-to-minute regulation of blood glucose. To design such a transplant, it is mandatory to have sufficient insight in factors influencing the kinetics of insulin secretion by encapsulated islets. The present study investi...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000003108747226
更新日期:2003-01-01 00:00:00
abstract::We investigated the effects of transplantation of CXCR4-overexpressing adipose tissue-derived stem cells (ADSCs) into a mouse diabetic hindlimb ischemia model on homing and engraftment as early as 48 h after transplant. CXCR4-overexpressing ADSCs were intramuscularly or intravenously injected into diabetic mice with h...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368916X692708
更新日期:2017-02-16 00:00:00
abstract::Spinocerebellar ataxia type 1 (SCA1) is a dominant inherited disease caused by expanded trinucleotide repeats resulting in an increased polyglutamine tract in the gene product. As a potential therapeutic approach for SCA1, we tested antisense RNAs targeting two regions of the ataxin-1 message. Single-stranded regions ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368908786516729
更新日期:2008-01-01 00:00:00
abstract::In multiple sclerosis (MS), a chronic inflammatory relapsing demyelinating disease, failure to control or repair damage leads to progressive neurological dysfunction and neurodegeneration. Implantation of neural stem cells (NSCs) has been shown to promote repair and functional recovery in the acute experimental autoi...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368911X637443
更新日期:2012-01-01 00:00:00
abstract::Ten mongrel dogs were used in this study. Diabetes was chemically induced in 7 dogs, and 3 dogs served as normal controls. For each diabetic dog, 5 million human bone marrow-derived mesenchymal stem cells/kg were differentiated to form insulin-producing cells using a trichostatin-based protocol. Cells were then loaded...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689718759913
更新日期:2018-06-01 00:00:00
abstract::Cell therapy has been shown to be a promising alternative therapy for neurorestoration, and more than 30 different types of cells have been shown to possess some capability to restore lost or damaged neurological functions. Chinese physicians have successfully applied cell therapy to many neurological diseases and dam...
journal_title:Cell transplantation
pub_type: 杂志文章,评审
doi:10.3727/096368913X672082
更新日期:2013-01-01 00:00:00
abstract::Large-scale sterile methods for isolating hepatocytes are desirable for the development of bioartificial liver support systems. In this study the traditional centrifuge method was compared with the use of a Baylor Rapid Autologous Transfusion (BRAT) machine for isolating large quantities of porcine hepatocytes. After ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000005783983007
更新日期:2005-01-01 00:00:00
abstract::Peripheral blood samples from HIV-seropositive individuals enrolled in a pilot clinical trial investigating the use of allogeneic dendritic cell therapy were evaluated for mixed chimerism. In this study, dendritic cells from HLA-identical, HIV-seronegative siblings were used. Patients received an infusion of dendritic...
journal_title:Cell transplantation
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:2000-05-01 00:00:00
abstract::Adrenal medullary tissue including chromaffin cells was grafted intrathecally in cancer patients to relieve intractable pain. The central nervous system (CNS) is considered an immune privileged site. Therefore, non-HLA-matched and unencapsulated tissue was grafted in 15 patients and 1 sham control in a series of at le...
journal_title:Cell transplantation
pub_type: 临床试验,杂志文章
doi:10.1177/096368970000900111
更新日期:2000-01-01 00:00:00
abstract::Intrauterine inflammation is a significant cause of injury to the developing fetal brain. Using a preterm fetal sheep model of in utero infection, we asked whether human amnion epithelial cells (hAECs) were able to reduce inflammation-induced fetal brain injury. Surgery was undertaken on pregnant sheep at ∼105 days ge...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368916X693572
更新日期:2017-04-13 00:00:00
abstract::Pluripotent stem cells, including human embryonic stem cells and induced pluripotent stem cells, have generated much excitement about their prospects for use in cell transplantation therapies. This is largely attributable to their virtually unlimited growth potential, their ability to be precisely genetically altered ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368910X498944
更新日期:2010-01-01 00:00:00
abstract::Circular RNAs (circRNAs) could sponge micro-RNAs (miRNAs) to regulate tumor progression of hepatocellular carcinoma (HCC). Hsa_circ_104566 contributes to papillary thyroid carcinoma progression. However, the tumorigenic mechanism of hsa_circ_104566 on HCC remains enigmatic. The role of hsa_circ_104566 on HCC was there...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689720963948
更新日期:2020-01-01 00:00:00
abstract::The common use of the marrow autograft mononuclear cell (MNC) count derives from positive correlative studies following allogeneic transplantation and from earlier conflicting data regarding the value of the bone marrow autograft colony-forming unit granulocyte-macrophage (CFU-GM) assay for prediction hematologic reco...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:
更新日期:1997-09-01 00:00:00