Synaptology of the cerebello-olivary pathway. Double labelling with anterograde axonal tracing and GABA immunocytochemistry in the rat.

Abstract:

:The dentato-olivary projection has been ultrastructurally studied in rats that received a wheatgerm agglutinin-horseradish peroxidase (WGA-HRP) injection in the nucleus lateralis. Ultrathin sections containing the inferior olive have been double-labelled with the GABA-immunogold method. About 97% of the WGA-HRP labelled axon terminals are GABA-immunopositive. Most of them belong to a single type consisting of small boutons establishing symmetrical synapses on dendrites. Nevertheless, there is some morphological and neurochemical diversity among the labelled terminals, and particularly, a small contingent are GABA-immunonegative. Of the GABAergic dentato-olivary boutons, 4% occupy a privileged position, with synaptic contacts straddling two dendritic profiles linked by gap junctions. The strategic location of these inhibitory dentato-olivary synapses suggests that they can modulate the electrotonic coupling rate between sets of inferior olivary neurons.

journal_name

Brain Res

journal_title

Brain research

authors

Angaut P,Sotelo C

doi

10.1016/0006-8993(89)91641-7

subject

Has Abstract

pub_date

1989-02-13 00:00:00

pages

361-5

issue

2

eissn

0006-8993

issn

1872-6240

pii

0006-8993(89)91641-7

journal_volume

479

pub_type

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