Abstract:
:Diabetes mellitus (DM) may lead to gastrointestinal motility disorders. Rodent models of DM indicate the presence of morpho-functional abnormalities of the enteric nervous system. Here, we evaluated whether experimental DM can cause changes in the excitatory cholinergic fibers, neuronal density, and voltage-gated sodium channel (Nav) expression in the myenteric plexus of the ileum. After streptozotocin-induced hyperglycemia in female rats progressed for eight weeks, triple immunofluorescence labeling experiments revealed that the neuronal density in DM rats was significantly lower than that in control. On average, the density of total neurons reduced by 52.2% (p = 0.0001), cholinergic neurons by 50.0% (p = 0.0068), and nitrergic neurons by 54.8% (p = 0.0042). The number of neurons per ganglionic area was also significantly reduced (to 28.2% of total neurons, p = 0.0002; 27.7% of cholinergic neurons, p = 0.0002, and 32.1% of nitrergic neurons, p = 0.0016). Furthermore, the density of the cholinergic fibers at the surface of the longitudinal muscle was significantly reduced (DM: 24 ± 3%; p = 0.003, control: 41 ± 2%); however, western-blot analysis did not indicate a reduction in the expression of choline acetyltransferase (ChAT) in the DM group. The Nav1.6 isoform was detected in different myenteric neurons of the ileum. RT-qPCR data did not suggest an alteration of transcripts for ChAT, neuronal nitric oxide synthase, Nav1.3, Nav1.6, or Nav1.7. Our data support the view that chronic DM leads to a reduction of excitatory cholinergic fibers and neuronal density. However, changes in sodium channel expression pattern, which could cause neuronal dysfunction, were not detected.
journal_name
Brain Resjournal_title
Brain researchauthors
Brasileiro AD,Garcia LP,de Carvalho da Silva S,Rocha LB,Pedrosa AL,Vieira AS,da Silva VJD,Rodrigues ARAdoi
10.1016/j.brainres.2018.11.041subject
Has Abstractpub_date
2019-04-01 00:00:00pages
1-9eissn
0006-8993issn
1872-6240pii
S0006-8993(18)30605-Xjournal_volume
1708pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract::The fate of dissociated neurons from 8-day chick embryo ciliary ganglia, cultured in serum-containing media on polyornithine substrata, is influenced by two different macromolecular factors. The neurons will die within 24 h in the absence of CNTF, the eye-derived ciliary neuronotrophic factor. Even when supported by C...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0165-3806(82)90175-4
更新日期:1982-12-01 00:00:00
abstract::High-intensity stimulation of the supraorbital region elicits, together with a blink reflex, a reflex inhibition of the jaw-closing muscles in normal man. The response differs from the well known inhibition obtained by intra- and perioral stimulation in two main features. Firstly, it consists of a single late silent p...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(86)90366-5
更新日期:1986-04-23 00:00:00
abstract::The effects of toluene on synaptic transmission and neuronal morphology were investigated using guinea pig hippocampal slices. Population spikes (PS) were elicited in granule cell layer by stimulation of the perforant path and antidromic potentials (AP) were evoked in the same locii by stimulation of mossy fibers. Tol...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(94)91983-6
更新日期:1994-11-21 00:00:00
abstract::Unilateral microinjections (0.20 microliter) of the excitatory amino acids (EAA), L-aspartate (ASP), D,L-homocysteate (DLH) or kainate (KA) were made into the midbrain of freely moving cats. Injections of DLH (20 nmol) or ASP (200 nmol) made within the midbrain periaqueductal grey matter (PAG) consistently elicited a ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(88)91465-5
更新日期:1988-01-26 00:00:00
abstract::To determine whether post-traumatic seizure severity would be affected by the interval between seizures and head injury, we measured seizures after various times with or without fluid percussion brain injury (2atm fluid percussion injury; FPI). To determine efficacy of anti-seizure medication, we also determined if le...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2016.04.013
更新日期:2016-07-01 00:00:00
abstract::Brain-derived neurotrophic factor (BDNF) is a well neurotrophic factor with neuroprotective potentials for various diseases in the central nervous system. However several previous studies demonstrated that BDNF might deteriorate symptoms for epilepsy model of animals by progression of abnormal neurogenesis. We hypothe...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2010.10.054
更新日期:2011-01-12 00:00:00
abstract::Erythropoietin (EPO) provides neuroprotection and neurorestoration after traumatic brain injury (TBI). The EPO doses used for treatment of TBI significantly increase hematocrit, which may affect the efficacy of EPO therapy for TBI. The aim of this study was to investigate whether normalization of hematocrit would affe...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2009.07.077
更新日期:2009-10-19 00:00:00
abstract::Activation of cAMP-dependent protein kinase (kinase A) has recently been shown to enhance responses evoked by stimulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors in cultured hippocampal pyramidal neurons. Here we report results of experiments designed to determine if activation of the...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(92)90803-h
更新日期:1992-03-06 00:00:00
abstract::We determined whether pentobarbital anesthetic is required to culture postmortem adult rat neurons. Pentobarbital treatment resulted in two-fold increases in neuron survival in culture after 2 and 4 h postmortem compared to non-anesthetic controls, but was not as effective as simple postmortem treatment on ice and the...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2004.03.006
更新日期:2004-05-29 00:00:00
abstract::Multiple-unit activity (MUA) in the agranular medial prefrontal cortex (PFC) was recorded in conscious rabbits during a classical conditioning procedure involving repeated pairings of a 4-s tone conditioned stimulus (CS) with eye shock. Prior to behavioral training, significant tone-evoked increases in prefrontal MUA ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(88)91435-7
更新日期:1988-02-23 00:00:00
abstract::In anaesthetized, spontaneously breathing cats and dogs, extracellular recordings were made of the spontaneous activities of 28 single neurones with cardiac rhythm. It was confirmed by histological examination that the neurones were situated in the mediodorsal portion of the nucleus of the solitary tract. Different di...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(77)90049-x
更新日期:1977-09-09 00:00:00
abstract::The mechanism(s) underlying age-associated diminutions in the rat striatal D2 dopamine receptor (D2-receptor) number was investigated. The levels of D2-receptor mRNA in 4-, 12- and 18-month-old rat striata were found not to change. In contrast, the levels of 110 kDa protein, labeled with a D2-receptor specific antibod...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(92)90095-q
更新日期:1992-03-20 00:00:00
abstract::We hypothesized that abnormal activity and adrenergic sensitivity in injured dorsal root ganglion (DRG) neurons are due to an intrinsic alteration of the cell body membrane. We investigated the effects of adrenergic stimulus on the activities of Ca2+ and K+ channels of DRG neurons in a rat chronic constriction injury ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(99)01499-7
更新日期:1999-06-19 00:00:00
abstract::Alterations occurring in the antioxidant enzymes, copper, zinc-dependent superoxide dismutase (Cu,Zn-SOD) and glutathione peroxidase (GPX) following nigral dopaminergic denervation are unclear. We now report on the distribution and levels of m-RNA for Cu,Zn-SOD and GPX in basal ganglia of normal and 1-methyl-4-phenyl-...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(03)02240-6
更新日期:2003-04-11 00:00:00
abstract::The effects of chronic administration of naloxone, morphine and met-enkephalin on benzodiazepine (BDZ) receptor binding in rat brain were determined 2 and 50 days after treatments were accomplished. Two days after naloxone treatment (75 micrograms/h s.c. for 14 days), enhanced BDZ receptor binding was observed in cing...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(93)91668-i
更新日期:1993-05-28 00:00:00
abstract::A daily variation of tryptophan hydroxylase (TrH) activity was observed in the raphe dorsalis (RD), raphe centralis (RC) and striatum (St) of 3 inbred strains of mice (BALB/c, C57BL/6, C57BR) and of the reciprocal hybrids obtained from Balb/c and C57BL6. Significant differences of the characteristics of these rhythms ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(80)90322-4
更新日期:1980-06-02 00:00:00
abstract::We sought to further elucidate signal transduction pathways for the I(1)-imidazoline receptor in PC12 cells and their interaction with the well-characterized signaling events triggered by nerve growth factor (NGF) in these cells. Stimulation of the I(1)-imidazoline receptor with moxonidine, a centrally acting antihype...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(03)02893-2
更新日期:2003-08-01 00:00:00
abstract::Incubation of rat striatal synaptosomes with the adenosine receptor agonist 2-chloroadenosine (2-CADO) produced a concentration-dependent increase of dopamine (DA) synthesis (about 50% of control value). The effect was not additive with the stimulation produced by either 10 microM forskolin or 2 mM dibutyryl cyclic AM...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(88)90232-6
更新日期:1988-07-26 00:00:00
abstract::Spontaneous intracerebral hemorrhage (ICH) is one of the most devastating subtypes of stroke. Since angiogenesis is a fundamental process to brain development and repair by new blood vessel formation from pre-existing ones, mediated by numerous angiogenic factors including vascular endothelial growth factor (VEGF), th...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2007.08.028
更新日期:2007-10-17 00:00:00
abstract::Sensitisation to the behavioural effects of amphetamine, a phenomenon which appears to involve the potentiation of excitatory amino acid (EAA)-mediated transmission at the level of dopaminergic (DA) neurons in the ventral tegmental area (the A10 cell group), is known to be affected by corticosteroid manipulations. Sin...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(96)00722-6
更新日期:1996-10-21 00:00:00
abstract::We investigated the effect of repetitive transcranial magnetic stimulation (rTMS) on long-term potentiation (LTP) in the rat hippocampus. Rats were magnetically stimulated at a rate of 1000 pulses/day for 7 days by a round coil, in which the peak magnetic fields at the center of the coil were 0.75 and 1.00 T. LTP enha...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2003.09.009
更新日期:2003-12-12 00:00:00
abstract::Axotomy produced an increase in the spike width and a decrease in amplitude of the afterhyperpolarization which followed the action potential in C-cells of bullfrog sympathetic ganglia. Although no change in B-cell conduction velocity was noted, C-cell conduction velocity increased significantly and approached that of...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(87)80044-6
更新日期:1987-04-28 00:00:00
abstract::Fluctuation analysis has been used to study the influence of temperature on the conductance and mean open time of Cl- permeable ion channels induced by gamma-aminobutyric acid (GABA) and glycine in the membrane of tissue cultured mouse spinal neurons. The conductance of GABA- and glycine-activated channels increased s...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(81)90875-1
更新日期:1981-11-16 00:00:00
abstract::The carrier-mediated uptake of anionic and cationic amino acids by the basolateral face (blood side) of the isolated perfused sheep choroid plexus was demonstrated using the paired-tracer dilution technique. The uptake of these two classes of amino acid was higher than at the blood-brain barrier with no cross-competit...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(92)90731-n
更新日期:1992-05-29 00:00:00
abstract::Phosphocreatine-Mg-complex acetate (PCr-Mg-CPLX) is a creatine-derived compound that in previous in vitro research was able to increase neuronal creatine independently of the creatine transporter, thus providing hope to cure the hereditary syndrome of creatine transporter deficiency. In previous research we showed tha...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2009.06.009
更新日期:2009-08-18 00:00:00
abstract::Acupuncture is widely used to relieve pain; however, the mechanism underlying electroacupuncture analgesia (EAA) during inflammatory pain is unclear. We investigated whether endogenous peripheral opioid receptors participated in EAA during hyperalgesia elicited by carrageenan-induced inflammation. Moreover, we investi...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2010.08.014
更新日期:2010-10-08 00:00:00
abstract::Horseradish peroxidase (HRP, 30% solution, 0.1-0.3 mul, 72 h) was injected unilaterally into the basal striatum (STR) and the dorsal ventricular ridge (DVR) of adult turtles (Chrysemys picta) in order to demonstrate the cells of origin of some afferents to these telencephalic structures. After selective STR injection,...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(76)90161-x
更新日期:1976-05-21 00:00:00
abstract::To characterize properties and mechanisms of non-photic entrainment of circadian rhythms, the effects of scheduled feeding were assessed in intact and suprachiasmatic nuclei (SCN) ablated C57BL/6j mice. During ad libitum food access, mice with no or partial SCN damage exhibited free-running activity and drinking rhyth...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(97)00571-4
更新日期:1997-08-15 00:00:00
abstract::Vascular dysfunction is important in the pathogenesis of peripheral complications of diabetes. However, the effects of diabetes on cerebral blood flow and the role of vascular deficits in the pathogenesis of diabetic encephalopathy are still unknown. The present study examined whether experimental diabetes is associat...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(02)04211-7
更新日期:2003-03-21 00:00:00
abstract::Some afferent fibers from the pudendal nerve of the female cat were stimulated by pressing on the perineal skin, the vagina or the uterine cervix. Three different types of skin mechanoreceptors were found: (1) with low threshold (< 20 mg) and slow-adapting discharges; (2) with high threshold (0.1-0.5 g) and slow-adapt...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(94)91589-x
更新日期:1994-08-29 00:00:00