Lectin reactivity of PNPF, a polyornithine-binding neurite-promoting factor.

Abstract:

:The fate of dissociated neurons from 8-day chick embryo ciliary ganglia, cultured in serum-containing media on polyornithine substrata, is influenced by two different macromolecular factors. The neurons will die within 24 h in the absence of CNTF, the eye-derived ciliary neuronotrophic factor. Even when supported by CNTF, however, ciliary neurons do not grow neurites unless the polyornithine substratum is coated with PNPF, a polyornithine-binding neurite-promoting factor. PNPF activity present in rat Schwannoma-conditioned medium has been shown to behave as a large, acidic, trypsin-sensitive molecule. In the experiments reported here the lectin reactivity of PNPF has been investigated. Using lectin affinity chromatography PNPF was found to bind to concanavalin A and wheat germ agglutinin from which it could be respectively eluted with the specific sugars alpha-methyl-D-mannoside and N-acetyl-D-glucosamine. PNPF did not bind to Ulex europaeus or Dolichus biflorus agglutinins. Pretreatment of polyornithine-bound PNPF with concanavalin A before cell seeding prevented neurite outgrowth from ciliary neurons in a dose-dependent manner, without affecting neuronal survival. This inhibitory effect of concanavalin A could be removed with alpha-methyl-D-mannoside. Wheat germ agglutinin failed to inhibit the neurite-promoting effects of polyornithine-bound PNPF.

journal_name

Brain Res

journal_title

Brain research

authors

Adler R,Manthorpe M,Varon S

doi

10.1016/0165-3806(82)90175-4

subject

Has Abstract

pub_date

1982-12-01 00:00:00

pages

69-75

issue

1

eissn

0006-8993

issn

1872-6240

journal_volume

282

pub_type

杂志文章
  • Termination of a separate (proprioceptive?) cuneothalamic tract from external cuneate nucleus in monkey.

    abstract::The termination of a pathway recently discovered in monkey, from the external cuneate nucleus (ECN) to the thalamus, has been investigated with the degeneration method. A previous HRP study showed that the projection is crossed. The ECN fibers terminate a wedge-shaped zone located dorsally in the rostral half of the c...

    journal_title:Brain research

    pub_type: 杂志文章

    doi:10.1016/0006-8993(81)90856-8

    authors: Boivie J,Boman K

    更新日期:1981-11-16 00:00:00

  • Prospects for cannabinoid therapies in viral encephalitis.

    abstract::Cannabinoids are promising therapies to support neurogenesis and decelerate disease progression in neuroinflammatory and degenerative disorders. Whether neuroprotective effects of cannabinoids are sustainable during persistent viral infection of the CNS is not known. Using a rodent model of chronic viral encephalitis ...

    journal_title:Brain research

    pub_type: 杂志文章

    doi:10.1016/j.brainres.2013.08.032

    authors: Solbrig MV,Fan Y,Hazelton P

    更新日期:2013-11-06 00:00:00

  • Microtubule organization and synaptogenesis in the vestibular sensory cells.

    abstract::Microtubule organization in type I hair cells has been investigated during the synaptogenesis of vestibular receptors in mammals. The different steps in the maturation of the synapse between the hair cell and the nerve chalice were: a slight symmetrical membrane densification; the appearance of synaptic bodies alongsi...

    journal_title:Brain research

    pub_type: 杂志文章

    doi:10.1016/0165-3806(86)90161-6

    authors: Favre D,Dememes D,Sans A

    更新日期:1986-02-01 00:00:00

  • Voluntary exercise increases neurotrophin-3 and its receptor TrkC in the spinal cord.

    abstract::We have evaluated changes in the expression of neurotrophin-3 (NT-3) and its tyrosine kinase C (TrkC) receptor in the neuromuscular system as a result of voluntary physical activity. We assessed changes in the mRNAs and proteins for NT-3 and TrkC in the lumbar spinal cord and associated soleus muscle following 3 and 7...

    journal_title:Brain research

    pub_type: 杂志文章

    doi:10.1016/s0006-8993(03)03258-x

    authors: Ying Z,Roy RR,Edgerton VR,Gómez-Pinilla F

    更新日期:2003-10-10 00:00:00

  • Differential effects of adenosine A1 receptor on pain-related behavior in normal and nerve-injured rats.

    abstract::This study investigated the effects of N6-cyclopentyladenosine (CPA), a potent and selective adenosine A1 receptor (A1R) agonist in normal and nerve-injured rats and mechanisms of its action by behavioral tests and electrophysiological technique. The results showed: (1) In normal rats, intraperitoneal administration o...

    journal_title:Brain research

    pub_type: 杂志文章

    doi:10.1016/j.brainres.2010.09.034

    authors: Gong QJ,Li YY,Xin WJ,Wei XH,Cui Y,Wang J,Liu Y,Liu CC,Li YY,Liu XG

    更新日期:2010-11-18 00:00:00

  • Alterations to the attention system in adults with tinnitus are modality specific.

    abstract::Generation and persistence of tinnitus following hearing loss may be due to aberrant engagement of attention. Here, functional MRI was used to determine differences in auditory and visual attention processing in adults with tinnitus and hearing loss compared to two age-matched control groups, one with matched hearing ...

    journal_title:Brain research

    pub_type: 杂志文章

    doi:10.1016/j.brainres.2015.05.010

    authors: Husain FT,Akrofi K,Carpenter-Thompson JR,Schmidt SA

    更新日期:2015-09-16 00:00:00

  • Losartan, nonpeptide angiotensin II-type 1 (AT1) receptor antagonist, attenuates pressor and sympathoexcitatory responses evoked by angiotensin II and L-glutamate in rostral ventrolateral medulla.

    abstract::We investigated the effect of losartan, a nonpeptide angiotensin II (Ang II)-type 1 (AT1) receptor antagonist, on the responses evoked by Ang II and L-glutamate (L-Glu) in the rostral ventrolateral medulla (RVLM). Adult spontaneously hypertensive rats (SHR) and Wistar-Kyoto (WKY) rats were anesthetized with halothane ...

    journal_title:Brain research

    pub_type: 杂志文章

    doi:10.1016/0006-8993(94)91344-7

    authors: Averill DB,Tsuchihashi T,Khosla MC,Ferrario CM

    更新日期:1994-12-05 00:00:00

  • Synchronous firing dynamics in a heterogeneous neurosecretory-cell population in an insect.

    abstract::Five pairs of neurosecretory cells in the subesophageal ganglion of the silkmoth Bombyx mori discharge action potentials in (near) synchrony to release a pheromone biosynthesis-activating neuropeptide (PBAN). Waveforms of compound action potentials recorded extracellularly from axonal tracts were analyzed to determine...

    journal_title:Brain research

    pub_type: 杂志文章

    doi:10.1016/s0006-8993(01)03349-2

    authors: Ichikawa T

    更新日期:2002-03-08 00:00:00

  • Pressor response to CSF sodium in mice: mediation by a ouabain-like substance and renin-angiotensin system in the brain.

    abstract::Intracerebroventricular (i.c.v.) infusion of sodium in rats increases cerebrospinal fluid (CSF) [Na], mimicking the effects of a high salt diet in salt-sensitive strains and causing sympathetic hyperactivity and a pressor response that are mediated via both an endogenous brain ouabainlike substance (OLS) and the brain...

    journal_title:Brain research

    pub_type: 杂志文章

    doi:10.1016/j.brainres.2004.06.056

    authors: Van Huysse JW,Hou X

    更新日期:2004-09-24 00:00:00

  • Cyclooxygenase 2 mRNA expression in rat brain after peripheral injection of lipopolysaccharide.

    abstract::Inducible cyclooxygenase 2 (COX 2) converts arachidonic acid to prostaglandins, which are thought to mediate various peripheral lipopolysaccharide (LPS)-induced central effects, including generation of fever and activation of the hypothalamic-pituitary-adrenal axis. To localize prostaglandin production in the brain fo...

    journal_title:Brain research

    pub_type: 杂志文章

    doi:10.1016/s0006-8993(98)00402-8

    authors: Quan N,Whiteside M,Herkenham M

    更新日期:1998-08-17 00:00:00

  • Angiotensin II excites neurones in cat solitary tract nuclei which are involved in respiration and related reflex activities.

    abstract::In anaesthetized cats, effects of iontophoretic application of angiotensin II were studied in the nuclei of the tractus solitarius. Specifically, its effects were tested on single respiratory neurones, having rhythmic activity related temporally with the phrenic nerve discharge, and on presumed reflex interneurones, w...

    journal_title:Brain research

    pub_type: 杂志文章

    doi:10.1016/0006-8993(87)91232-7

    authors: Sessle BJ,Henry JL

    更新日期:1987-03-24 00:00:00

  • Role of central nervous system neurotransmitters in mediating the effects of morphine on growth hormone- and prolactin-secretion in the rat.

    abstract::Unanesthetized adult male rats with indwelling right atrial cannulae were used in the majority of experiments. Morphine (MOR, 3.0 mg/kg) caused a large but transient increase in both GH and PRL levels, which could be prevented with naloxone. Disruption of central noradrenergic function with diethyldithiocarbamate (400...

    journal_title:Brain research

    pub_type: 杂志文章

    doi:10.1016/0006-8993(80)91130-0

    authors: Koenig J,Mayfield MA,Coppings RJ,McCann SM,Krulich L

    更新日期:1980-09-22 00:00:00

  • Effect of external acidosis on basal and ATP-evoked calcium influx in cultured astrocytes.

    abstract::The effect of lactic acidosis on calcium influx, accumulation and efflux was studied in primary cultures of neonatal cortical rat astrocytes. Treatment of cultures with 20 mM sodium lactate, pH 6.0, for 10-60 min resulted in a 35% reduction of 45Ca2+ influx. The decrease in calcium influx was pH dependent because a si...

    journal_title:Brain research

    pub_type: 杂志文章

    doi:10.1016/0006-8993(93)90371-s

    authors: Neary JT,Fu Q,Bender AS,Norenberg MD

    更新日期:1993-02-26 00:00:00

  • Three-dimensional mapping of norepinephrine and serotonin in human thalamus.

    abstract::Detailed quantitative information on catecholamines and 5-hydroxytryptamine (serotonin) in the human thalamus is much needed because of increasing interest in norepinephrine and serotonin as modulators of thalamic behavioral state control and overall information processing. This study provides three-dimensional distri...

    journal_title:Brain research

    pub_type: 杂志文章

    doi:10.1016/s0006-8993(97)00404-6

    authors: Oke AF,Carver LA,Gouvion CM,Adams RN

    更新日期:1997-07-18 00:00:00

  • Reduced anxiety-like and cognitive performance in mice lacking the corticotropin-releasing factor receptor 1.

    abstract::Corticotropin-releasing factor (CRF) has been hypothesized to be involved in the pathophysiology of anxiety, depression, cognitive and feeding disorders. Two distinct CRF receptor subtypes, CRFR1 and CRFR2, are thought to mediate CRF actions in the CNS. However, the role for each receptor subtype in animal models of n...

    journal_title:Brain research

    pub_type: 杂志文章

    doi:10.1016/s0006-8993(98)01158-5

    authors: Contarino A,Dellu F,Koob GF,Smith GW,Lee KF,Vale W,Gold LH

    更新日期:1999-07-17 00:00:00

  • Gangliosides modulate Schwann cell proliferation and morphology.

    abstract::We examined the effect of gangliosides on Schwann cell cultures isolated from neonatal rat sciatic nerves. Addition of gangliosides (GM1, GM3, and ganglioside mixture) at concentrations between 0.25 and 2 mg/ml significantly diminished both the baseline rate of proliferation of the Schwann cells and their response to ...

    journal_title:Brain research

    pub_type: 杂志文章

    doi:10.1016/0006-8993(88)90442-8

    authors: Sobue G,Taki T,Yasuda T,Mitsuma T

    更新日期:1988-12-06 00:00:00

  • Habituation of the local cyclic GMP response during amygdaloid carbachol kindling in the rat.

    abstract::Seizures kindled with amygdaloid carbachol injections are transynaptic, dependent on activation of a specific population of muscarinic receptors, and some components of their expression could be mediated by intracellular second messengers. We measured cyclic GMP and cyclic AMP concentrations in micropunch biopsies of ...

    journal_title:Brain research

    pub_type: 杂志文章

    doi:10.1016/0006-8993(86)90637-2

    authors: Wasterlain CG,Csiszar E,Fairchild MD

    更新日期:1986-11-12 00:00:00

  • Effects of intraventricular 6-hydroxydopamine injections on serum prolactin and LH levels: absence of stress-induced pituitary prolactin release.

    abstract::The drug 6-hydroxydopamine (6-OHDA) has been reported to reduce hypothalamic norepinephrine (NE) content after administration into the lateral ventricle without altering the dopamine content of tubero-infundibular neurons. Serum prolactin levels in male rats injected with 2 X 250 mug 6-OHDA were significantly higher t...

    journal_title:Brain research

    pub_type: 杂志文章

    doi:10.1016/0006-8993(76)90647-8

    authors: Fenske M,Wuttke W

    更新日期:1976-03-05 00:00:00

  • Inhibition of the soleus H-reflex in standing man.

    abstract::There exists evidence to support the notion that the segmental reflex system is not fixed and inflexible, but rather is highly modifiable under a variety of circumstances. In this study the H-wave and M-wave recruitment curves were obtained from 19 subjects, utilizing the procedures outlined by Hugon. Each subject was...

    journal_title:Brain research

    pub_type: 杂志文章

    doi:10.1016/0006-8993(93)90495-9

    authors: Koceja DM,Trimble MH,Earles DR

    更新日期:1993-11-26 00:00:00

  • Metabolic and behavioral deficits following a routine surgical procedure in rats.

    abstract::To test the hypothesis that functional metabolic deficits observed following surgical brain injury are associated with changes in cognitive performance in rodents, we performed serial imaging studies in parallel with behavioral measures in control animals and in animals with surgical implants. Memory function was asse...

    journal_title:Brain research

    pub_type: 杂志文章

    doi:10.1016/j.brainres.2007.01.134

    authors: Frumberg DB,Fernando MS,Lee DE,Biegon A,Schiffer WK

    更新日期:2007-05-04 00:00:00

  • Immunolocalization of the Na(+)-Ca2+ exchanger in mammalian myelinated axons.

    abstract::Previous studies on the pathophysiology of white matter anoxic injury have revealed that the Na(+)-Ca2+ exchanger is an important mediator of Ca2+ overload. To date, however, the localization of this key Ca2+ transporter in myelinated axons has not been demonstrated. The present study uses immunofluorescence labeling ...

    journal_title:Brain research

    pub_type: 杂志文章

    doi:10.1016/s0006-8993(97)00868-8

    authors: Steffensen I,Waxman SG,Mills L,Stys PK

    更新日期:1997-11-21 00:00:00

  • Proximal segment retraction increases the probability of nerve cell survival after dendrite transection.

    abstract::Dendrites were transected 100 microns from nerve cell somata. The probability of cell survival increased as a function of the extent of proximal segment retraction. The retraction effect is probably the result of increased resistance to calcium and sodium currents at the lesion. It is speculated that limitation of ner...

    journal_title:Brain research

    pub_type: 杂志文章

    doi:10.1016/0006-8993(87)90526-9

    authors: Lucas JH

    更新日期:1987-11-10 00:00:00

  • Caspase activation contributes to astrogliosis.

    abstract::Caspases, a family of cysteine proteases, are widely activated in neurons and glia in the injured brain, a response thought to induce apoptosis. However, caspase activation in astrocytes following injury is not strongly associated with apoptosis. The present study investigates the potential role of caspase activation ...

    journal_title:Brain research

    pub_type: 杂志文章

    doi:10.1016/j.brainres.2012.02.056

    authors: Aras R,Barron AM,Pike CJ

    更新日期:2012-04-23 00:00:00

  • Evolution of Bergmann glia in developing human fetal cerebellum: a Golgi, electron microscopic and immunofluorescent study.

    abstract::Astrogliogenesis in the human fetal cerebellum was examined in 46 cerebella obtained from hysterotomy specimens ranging between 9 and 20 weeks of ovulation age. By correlating the results obtained by rapid Golgi and Golgi-Cox methods, the indirect immunofluorescence technique for glial fibrillary acidic protein, and e...

    journal_title:Brain research

    pub_type: 杂志文章

    doi:10.1016/0006-8993(80)90280-2

    authors: Choi BH,Lapham LW

    更新日期:1980-05-26 00:00:00

  • Intraventricular carbachol mimics the phase-shifting effect of light on the circadian rhythm of wheel-running activity.

    abstract::Intraventricular injections of carbachol, a cholinergic agonist, into free-running mice can cause phase dependent phase shifts, in both directions, in the circadian rhythm of wheel-running activity. These effects mimic the action of light in entraining the circadian pacemaker, and suggest that acetylcholine may play a...

    journal_title:Brain research

    pub_type: 杂志文章

    doi:10.1016/0006-8993(81)90059-7

    authors: Zatz M,Herkenham MA

    更新日期:1981-05-11 00:00:00

  • Serotonergic modulation of reward and punishment: evidence from pharmacological fMRI studies.

    abstract::Until recently, the bulk of research on the human reward system was focused on studying the dopaminergic and opioid neurotransmitter systems. However, extending the initial data from animal studies on reward, recent pharmacological brain imaging studies on human participants bring a new line of evidence on the key rol...

    journal_title:Brain research

    pub_type: 杂志文章,评审

    doi:10.1016/j.brainres.2014.02.003

    authors: Macoveanu J

    更新日期:2014-03-27 00:00:00

  • MDMA (ecstasy) modulates locomotor and prefrontal cortex sensory evoked activity.

    abstract::Ingestion of 3, 4-methylenedioxymethamphetamine (MDMA) leads to heightened response to sensory stimulation; thus, MDMA is referred to as "ecstasy" because it produces pleasurable enhancement of such sensation. There have been no electrophysiological studies that report the consequences of MDMA on sensory input. The pr...

    journal_title:Brain research

    pub_type: 杂志文章

    doi:10.1016/j.brainres.2009.09.048

    authors: Atkins K,Burks T,Swann AC,Dafny N

    更新日期:2009-12-11 00:00:00

  • Pharmacological characterization of perifornical hypothalamic dopamine receptors mediating feeding inhibition in the rat.

    abstract::Mapping studies with central drug injections in the hungry rat have identified the perifornical lateral hypothalamus as being uniquely sensitive to the feeding suppressive effects of exogenous dopamine, as well as anorexic drugs which release endogenous catecholamines. In the present study, the hypothalamic dopamine-r...

    journal_title:Brain research

    pub_type: 杂志文章

    doi:10.1016/0006-8993(79)90899-0

    authors: Leibowitz SF,Rossakis C

    更新日期:1979-08-17 00:00:00

  • Spatial distribution of corticotropin-releasing factor immunopositive climbing fibers in the mouse cerebellum: analysis by whole mount immunohistochemistry.

    abstract::This study examined the spatial organization of corticotropin-releasing factor (CRF) immunopositive climbing fibers in the mouse cerebellum by whole mount immunohistochemistry. A striking pattern of parasagittal stripes of CRF staining was revealed. Cryosections of whole mount CRF stained cerebellum showed that anti-C...

    journal_title:Brain research

    pub_type: 杂志文章

    doi:10.1016/j.brainres.2008.05.029

    authors: Sawada K,Fukui Y,Hawkes R

    更新日期:2008-07-30 00:00:00

  • Substance P: depletion in the dorsal horn of rat spinal cord after section of the peripheral processes of primary sensory neurons.

    abstract::The substance P content, glutamic acid decarboxylase and choline acetyltransferase activities and the level of [3H]diprenorphine binding were measured in various regions of the lumbar spinal cord of rats after unilateral section of the sciatic nerve or after dorsal rhizotomy. Sciatic nerve section produced a 75--80% d...

    journal_title:Brain research

    pub_type: 杂志文章

    doi:10.1016/0006-8993(79)90167-7

    authors: Jessell T,Tsunoo A,Kanazawa I,Otsuka M

    更新日期:1979-05-25 00:00:00