Abstract：
:Two high molecular weight surface glycoproteins of cerebellar cells which are selectively labeled by lactoperoxidase-catalyzed iodination of monolayer cultures from the developing mouse cerebellum, have been identified and partially characterised. Both molecules, called peak 2 and peak 3 proteins, were the major glycoprotein species detected in cerebellar cell cultures after labelling with various radioactive sugars. The freshly iodinated molecules were firmly bound to the cells, but they were released into the medium upon prolonged incubation on the cultures. The soluble peak 2 and peak 3 proteins recovered from the medium comigrated on SDS-polyacrylamide gels with their cell-bound counterparts. Thus, their release results from mechanisms other than extensive degradation. The soluble proteins eluted from gel columns corresponding to molecular weights of over 500,000 and around 300,000, for peaks 2 and 3, respectively. They bound to and were specifically eluted from concanavalin A-Sepharose columns. Peak 3 protein could be easily identified as the most prominent iodinatable polypeptide in cerebellar cell cultures. Its surface expression depended on the presence of neuronal cells. After degeneration of neuron-like cells, a component of greater molecular weight than peak 3 or 2 was predominantly labeled by surface iodination. Peak 2 protein was quantitatively precipitated from labeled culture medium by two heterologous antiseRA. Anti-peak 2 activity was removed from the antiserum by absorption with adult mouse brain, but not by liver, spleen, thymus, kidney, heart and lung. Thus, peak 2 protein may be considered as a brain-specific glycoprotein.
journal_name
Brain Resjournal_title
Brain researchauthors
Goridis C,Hirsch M,Dossetto M,Baechler Edoi
10.1016/0006-8993(80)91197-xsubject
Has Abstractpub_date
1980-01-27 00:00:00pages
397-414issue
2eissn
0006-8993issn
1872-6240pii
0006-8993(80)91197-Xjournal_volume
182pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract：:Surgical tissue samples of the human dentate gyrus were immunostained for glutamate decarboxylase (GAD), the gamma-aminobutyric acid (GABA)-synthesizing enzyme, and studied by both light and electron microscopy. Immunoreactive neurons and terminals displayed similar morphological characteristics as known from studies ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(87)91181-4
更新日期：1987-01-13 00:00:00
abstract：:Rat striata were exposed to 15 mM quinolinic acid (QUIN), or QUIN plus the nitric oxide synthase inhibitors S-methyl-L-thiocitrulline dihydrochloride (L-MIN) or 7-nitroindazole monosodium salt (7-NINA) for 21 days. Co-administration of 100 microM or 1 mM L-MIN with QUIN significantly reduced lesion volume compared to ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(97)00962-1
更新日期：1997-11-14 00:00:00
abstract：:ACTH4-10, a fragment of the adrenocorticotropic hormone (ACTH) molecule, has marked effects on the compensation process following unilateral labyrinthectomy. In Rana temporaria ACTH4-10-treatment (5-250 micrograms/kg) influences both the acquisition and the maintenance of the compensated state. The compensation proces...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(82)91158-1
更新日期：1982-09-23 00:00:00
abstract：:Porcine calcitonin (pCT) encapsulated in sulfatide-containing reverse-phase evaporation vesicles (REVs) was shown to induce long-lasting and dose-dependent antinociceptive effect by systemic injection in mice. Its antinociceptive activity is found to be dependent on the route of administration. In contrast, neither un...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(93)91311-f
更新日期：1993-02-12 00:00:00
abstract：:Experiments were undertaken to compare effects of the NMDA and non-NMDA receptor antagonists, AP5 (40 microM) and NBQX (10 microM), on glutamate-induced firing in supraoptic oxytocin (OT) and vasopressin (VP) neurones in vitro. In putative OT neurones NBQX caused a significantly greater reduction in firing than AP5, w...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(97)00728-2
更新日期：1997-08-29 00:00:00
abstract：:Stress can precipitate onset of multiple mood-related disorders, including depression. Examination of the neural basis of this phenomenon has highlighted the amygdala as a key component. Alterations in amygdalar activity and structure accompany various mood-related disorders, and interestingly, amygdalar morphology an...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2008.12.022
更新日期：2009-02-23 00:00:00
abstract：:Murine cortical cell cultures deprived of glucose for 6-8 h developed extensive neuronal degeneration, apparent both morphologically and by efflux of lactate dehydrogenase to the bathing medium. This neuronal damage could be substantially reduced by addition of D-2-amino-5-phosphonovalerate (D-APV), in a concentration...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(89)90179-0
更新日期：1989-04-03 00:00:00
abstract：:The current study explored the effects of prolonged antidepressant treatment on mRNA levels of two nuclear receptors in specific brain regions of an animal model of depression, the Wistar-Kyoto (WKY) rat. Both nuclear receptors have been implicated in the development or treatment of depression. The expression of nucle...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2010.09.041
更新日期：2010-11-29 00:00:00
abstract：:This study was performed to understand the anatomical substrates of hypothalamic modulation of jaw movements. After cholera toxin B subunit (CTb) injection into the parvicellular reticular formation (RFp) of the rat medulla oblongata, where many trigeminal premotor neurons have been known to exist, numerous CTb-labele...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2008.09.076
更新日期：2008-12-09 00:00:00
abstract：:Animal models of cerebral infarction are crucial to understanding the mechanisms of neuronal survival following ischemic brain injury and to the development of therapeutic interventions for victims of all types of stroke. Rodents have been used extensively in such research. One rodent model of stroke utilizes either p...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2005.02.021
更新日期：2005-04-25 00:00:00
abstract：:Subcutaneous administration of rotenone has recently attracted attention because of its convenience, simplicity and efficacy in replicating features of Parkinson's disease (PD) in animal models. However, the wide range of doses reported in the literature makes it difficult to evaluate the effectiveness of this techniq...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2016.11.020
更新日期：2017-01-15 00:00:00
abstract：:To investigate whether differences in vulnerability to free radicals might underlie differences among striatal neurons in their vulnerability to neurodegenerative processes such as occur in ischemia and Huntington's disease, we have analyzed the localization of superoxide free radical scavengers in different striatal ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(95)01320-2
更新日期：1996-02-05 00:00:00
abstract：:The striatum has nearly equal numbers of striatonigral and striatopallidal projection neurons. All are GABAergic and inhibitory, but they lie in separate neuronal circuits ('direct' and 'indirect', respectively) that appear to exert opposite effects on movement. Methods are needed to evaluate the function of each circ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(00)03170-x
更新日期：2001-03-09 00:00:00
abstract：:Using a monoclonal antibody (192-IgG) directed against the rat beta-nerve growth factor (beta-NGF) receptor the distribution of beta-NGF receptors in the forebrain of the rat has been determined. beta-NGF receptor-containing cells were located in presumed cholinergic cells of the medial septal nucleus, vertical and ho...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(88)91175-4
更新日期：1988-02-02 00:00:00
abstract：:There is a high incidence of cardiac arrest and poorer post-resuscitation outcome in the elderly population. Cardiac arrest and resuscitation results in ischemia/reperfusion injury associated with oxidative stress, leading to post-resuscitation mortality and delayed selective neuronal cell loss. In this study we inves...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2010.02.080
更新日期：2010-04-30 00:00:00
abstract：:Alzheimer's disease (AD) classically presents with gray matter atrophy, as well as feature significant white matter abnormalities. Previous evidence indicates the overall burden of these pathological changes continues to advance as the disease progresses. The aim of this study was to investigate whether pathological a...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2013.03.052
更新日期：2013-06-17 00:00:00
abstract：:In the present study we examined the laminar distributions of four types of chemically defined subpopulations of non-principal neurons, that is, those immunoreactive for parvalbumin (PV), calretinin (CR), nitric oxide synthase (NOS) and somatostatin (SS), in the rat hippocampus, by estimating their approximate numeric...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(97)00457-5
更新日期：1997-08-01 00:00:00
abstract：:Following transection of a peripheral nerve in mice, a newly synthesized neurotropic pyrimidine compound, MS-818 was administered intraperitoneally at a dose of 1 mg kg-1 b.wt. day-1. The film model experiments for analyzing the early growth of axonal regeneration suggested that MS-818 activated Schwann cells which mi...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(97)01491-1
更新日期：1998-03-23 00:00:00
abstract：:The effects of alpha-bungarotoxin from Bungarus multicinctus and Bungarus caeruleus were studied on three types of cholinergic response in Aplysia central neurones. These responses are the result of three distinct changes in ionic permeability: selective increases in permeabiliyt to Na, Cl and K, respectively. It was ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(76)90142-6
更新日期：1976-05-14 00:00:00
abstract：:To test the hypothesis that there is a functional modulation of conventional EEG bands associated with preparatory attention, putative changes in the spontaneous brain rhythms and their associated cerebral sources were addressed. The goals of the present report were, first, to find the brain areas with maximal rhythmi...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2006.06.019
更新日期：2006-08-30 00:00:00
abstract：:Androgens are involved in a variety of centrally mediated functions after binding to their intracellular receptors. In the present report, we have employed the androgen receptor antibody, PG-21, and indirect immunohistochemistry to examine the distribution of cells containing androgen receptor-like immunoreactivity (A...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(95)00983-3
更新日期：1995-12-12 00:00:00
abstract：:In the dorsal horn of barbiturate-anaesthetized cats evidence was obtained for a different distribution of enkephalin-preferring and morphine-preferring receptors. When ejected electrophoretically from micropipettes, morphine reduced the nociceptive responses of neurones of laminae IV and V when administered in the su...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(81)91002-7
更新日期：1981-12-21 00:00:00
abstract：:Lateral ventricular injection of antibodies to dynorphin A(1-13) was previously shown to elevate lateral hypothalamic stimulation frequency threshold for eliciting feeding behavior. The antibodies utilized in that study cross-react completely with dynorphin A(1-17) and, to a lesser extent, dynorphin A(1-8). In the pre...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(90)90284-i
更新日期：1990-01-22 00:00:00
abstract：:The localization of human hand primary motor area (M1) has been the object of several studies during the last decades. EEG source analysis, functional magnetic resonance imaging (fMRI) and focal transcranial magnetic stimulation (TMS) are non-invasive methods for localizing M1 with good accuracy compared to direct ele...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2009.10.042
更新日期：2010-01-13 00:00:00
abstract：:Single neurons in culture express a heterogeneity of neurotransmitter receptor subtypes. The study of the effects of neurotransmitters on neuronal function is complicated by this heterogeneity. It would therefore be useful to be able to identify live neurons that express the receptors of interest and then use these ne...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(96)00436-2
更新日期：1996-08-26 00:00:00
abstract：:The presence of adrenocorticotropic hormone (ACTH)-immunoreactive cells and melanocortin (MC) receptors (MC4 and to a lesser extent MC3) has been demonstrated in the medullary reticular formation in the general area where rostral ventrolateral medullary pressor area (RVLM) is located. The importance of RVLM in the reg...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2006.05.002
更新日期：2006-08-02 00:00:00
abstract：:Benzodiazepine binding sites are present on gamma-aminobutyric acid (GABA) receptors in hippocampal neurons. Diazepam is known to potentiate the amplitude and prolong the decay of GABA(A) receptor-mediated inhibitory postsynaptic currents (IPSCs). In this study, benzodiazepine involvement in long-term potentiation (LT...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2005.09.008
更新日期：2005-11-16 00:00:00
abstract：:Previous studies resulted in conflicting conclusions that glutamate application either decreases or increases the activity of Ca2+ channels in hippocampal neurons. We studied whole-cell Ca2+ currents (ICa) in chick dorsal root ganglion neurons and rat hippocampal cells. For both cell types glutamate (1-30 microM) incr...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(92)90965-c
更新日期：1992-05-15 00:00:00
abstract：:Immunohistochemical localization of brain-derived neurotrophic factor (BDNF) in the monkey brain was investigated using a polyclonal anti-BDNF antibody produced in our laboratory. The antibody recognized a single band in monkey brain homogenates, and the estimated molecular weight was approximately 14 kDa, which corre...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(99)01082-3
更新日期：1999-03-13 00:00:00
abstract：:Niemann-Pick Type C (NPC) disease is an autosomal recessive neurodegenerative disorder with abnormal lipid storage as the major cellular pathologic hallmark. Genetic analyses have identified mutations in NPC1 gene in the great majority of cases, while mutations in NPC2 account for the remainders. Yet little is known r...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2010.02.019
更新日期：2010-04-14 00:00:00
