Abstract：
:Single neurons in culture express a heterogeneity of neurotransmitter receptor subtypes. The study of the effects of neurotransmitters on neuronal function is complicated by this heterogeneity. It would therefore be useful to be able to identify live neurons that express the receptors of interest and then use these neurons for functional studies. We have used quantitative fluorescence microscopy to identify single live striatal neurons that express D1 dopamine receptors. The binding of the fluorescent D1 dopamine receptor antagonist bodipy-SCH 23390 was measured in 2-3-week-old primary striatal cultures derived from fetal rats (embryonic day 18). Binding of bodipy-SCH 23390 to live neurons was displaced by (+)-butaclamol, dopamine or SCH 23390, indicating that it specifically labelled D1 dopamine receptors. However, the fraction of bodipy-SCH 23390 binding that was specific varied substantially among individual neurons indicating heterogeneity of D1 dopamine receptor expression. Interestingly, bodipy-SCH 23390 also specifically labelled discrete spots of receptors on the neuronal processes. This technique should prove useful in the study of the effects of dopaminergic drugs on neuronal function in primary culture.
journal_name
Brain Resjournal_title
Brain researchauthors
Hoyt KR,Reynolds IJdoi
10.1016/0006-8993(96)00436-2subject
Has Abstractpub_date
1996-08-26 00:00:00pages
21-30issue
1-2eissn
0006-8993issn
1872-6240pii
0006-8993(96)00436-2journal_volume
731pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract：:The cloned 5-HT3 receptor from NCB-20 neuroblastoma cells was expressed in Xenopus oocytes. In these oocytes, 5-HT, the selective 5-HT3 receptor agonists, 2-methyl-5-HT and m-chlorophenylbiguanide activated an inward current which was sensitive to the specific 5-HT3 receptor antagonist LY278584. Cocaine (0.1 to 10 mic...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(94)01316-a
更新日期：1995-03-06 00:00:00
abstract：:We have previously shown that systemic administration of ondansetron, a selective serotonin type-3 (5-HT3) receptor antagonist, attenuates cholecystokinin (CCK)-induced suppression of food intake. The exact location of 5-HT3 receptors mediating this action is not clear and may involve hindbrain 5-HT3 receptors. In thi...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2006.05.058
更新日期：2006-08-04 00:00:00
abstract：:The herpes virus suis has been used as a tracer for the pathways in the central nervous system of the rat. The viruses have been inoculated in various peripheral structures innervated by the trigeminal nerve, namely in the cornea by instillation or scarification, in the anterior chamber of the eye by injection and als...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(83)90850-8
更新日期：1983-08-29 00:00:00
abstract：:The functional organization of projections from the cerebellar subcortical nuclei to the motor cortex through the nucleus ventralis lateralis (VL) of the thalamus was studied using tungsten microelectrodes for stimulation and recordings in acute and chronic cats. The following results were obtained. (1) Microstimulati...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(75)90510-7
更新日期：1975-11-07 00:00:00
abstract：:The present study sought to determine if axonal connectivity is established between 6-month-old neostriatal transplants and the host substantia nigra. Cell suspensions of fetal neostriatum were transplanted into the adult rat neostriatum lesioned previously by kainic acid. Horseradish peroxidase injections into the ip...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(89)91256-0
更新日期：1989-01-09 00:00:00
abstract：:Glutamate is the main excitatory neurotransmitter in the central nervous system. This amino acid mediates learning and memory processes acting through ionotropic and metabotropic receptor binding. Metabotropic glutamate receptors (mGluRs) are G protein-coupled receptors that stimulate phospholipase C (PLC) or inhibit ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2007.01.010
更新日期：2007-06-11 00:00:00
abstract：:Strong evidence obtained from in vivo and ex-vivo studies suggests the existence of interaction between dopaminergic and nitrergic systems. Some of the observations suggest a possible implication of nitric oxide (NO) in dopamine (DA) uptake mechanism. The present work investigated the interaction between both systems ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2007.12.054
更新日期：2008-03-10 00:00:00
abstract：:Adaptive decision making in dynamic social settings requires frequent re-evaluation of choice outcomes and revision of strategies. This requires an array of multiple cognitive abilities, such as working memory and response inhibition. Thus, the disruption of such abilities in schizophrenia can have significant implica...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2007.08.049
更新日期：2007-11-14 00:00:00
abstract：:Serotonin (5HT) systems play important roles in brain development, and early perturbations of 5HT receptor expression produce permanent changes in 5HT synaptic function and associated behaviors. We exposed pregnant Rhesus monkeys to environmental tobacco smoke (ETS) during gestation and for up to 3 months postnatally ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2006.06.094
更新日期：2006-09-21 00:00:00
abstract：:The axonal arborizations of the basket cells in the cerebral neocortex have long been considered as the source of the presynaptic terminals contacting the cell bodies of pyramidal cells. Given that the concept of the cortical basket cell is based upon indirect evidence only, it was deemed worthwhile to re-investigate ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(82)90898-8
更新日期：1982-07-22 00:00:00
abstract：:Developmental delay, cognitive impairment, and refractory epilepsy are the most frequent consequences found in patients suffering from malformations of cortical development (MCD). However, therapeutic options for these psychiatric and neurological comorbidities are currently limited. The development of white matter un...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2015.07.012
更新日期：2015-10-05 00:00:00
abstract：:Activity produced by direct microelectrophoretic application of glutamate onto 19 convergent neurones in trigeminal nucleus caudalis, was strongly depressed during and after the application of heterotopic noxious conditioning stimuli: noxious heat (52 degrees C) applied to the tail, noxious pinches applied to the tail...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(84)90695-4
更新日期：1984-10-29 00:00:00
abstract：:Severed medial giant axons in crayfish can be rejoined in vitro with polyethylene glycol (PEG) to produce axoplasmic continuity and through transmission of action potentials. Severed axon-like processes of a mammalian neuroblastoma/glioma cell line seem to be rejoined to the cell body using PEG in tissue culture. Our ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(86)91617-3
更新日期：1986-03-05 00:00:00
abstract：:Gastrin-releasing peptide (GRP) mediated signals in the central nervous system (CNS) influence many functions associated with energy metabolism. The purpose of the present study was to investigate the central effect of GRP on glucose metabolism in the male rat. Intracerebroventricular (icv) administration of GRP cause...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2015.08.040
更新日期：2015-11-02 00:00:00
abstract：:This study tested the hypothesis that the short-latency excitation of the phrenic motor output elicited by superior laryngeal nerve (SLN) stimulation requires non-NMDA receptor-mediated neurotransmission in the region of the dorsal respiratory group (DRG) of the adult cat. Injection of the non-NMDA receptor antagonist...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(95)00355-t
更新日期：1995-06-05 00:00:00
abstract：:Measurement of histamine and its metabolizing enzymes in a variety of chordate species indicated that histamine and histamine methyltransferase were present in brain of all vertebrate species with a recognizable brain structure. Diamine oxidase was absent in mammalian brain but was present in brain of lower vertebrate...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(81)90642-9
更新日期：1981-03-09 00:00:00
abstract：:The recent development of rodent models of bone cancer pain has started to provide the basis for demonstrating the particular neurochemical and behavioral entity of cancer pain. Behaviourally, both spontaneous pain and hyperalgesia related to mechanical, but not thermal, noxious stimuli have been described in cancer-b...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(03)02284-4
更新日期：2003-04-18 00:00:00
abstract：:This study used microdialysis to monitor extracellular levels of 5-HT and its metabolite, 5-hydroxyindole acetic acid (5-HIAA) in the lateral hypothalamus (LH) and hippocampus of freely moving rats that had developed a CTA to a 2.5 mM saccharin solution (CS) following its pairing with illness induced by lithium chlori...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(91)90551-6
更新日期：1991-08-09 00:00:00
abstract：:The release of adipokinetic hormones from the neurosecretory cells within the glandular lobe of the corpus cardiacum (CC) of locusts is under the synaptic control of axons within the nervi corporis cardiaca II (NCC II). This synapse has been shown to be aminergic with the transmitter tentatively identified as octopami...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(83)91242-8
更新日期：1983-01-03 00:00:00
abstract：:Previous studies have revealed that there are increases in the incorporation of [3H]amino acids into protein in the denervated neuropil of the dentate gyrus during periods of reactive synaptogenesis. The present study evaluates whether the increase in incorporation reflects an increase in protein synthetic machinery (...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0169-328x(87)90032-5
更新日期：1987-09-01 00:00:00
abstract：:The objective was to obtain detailed topographic determinations of cytochrome oxidase activity in the gerbil central auditory system at the light microscopic level. Quantitative techniques were developed using (1) tissue standards calibrated to express histochemical measures as actual enzyme activity units, (2) densit...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(94)90836-2
更新日期：1994-10-10 00:00:00
abstract：:The Wistar Audiogenic Rat (WAR) is a genetic model of reflex epilepsy with seizures induced by high-intensity sound stimulation (120 dB SPL). In spite of the known neural substrates involved in WAR seizure phenotype, neuroendocrine hypothalamic neurons were never investigated. In this work, AVP immunohistochemistry in...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2004.12.030
更新日期：2005-03-15 00:00:00
abstract：:Results from the application of a procedure that allows simultaneous estimation of the rates of glucose transport from blood and of glucose phosphorylation in discrete regions of the brain are given for groups of rats displaying chemically induced motor disturbances. The procedure is based on sequential injections of ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(81)90782-4
更新日期：1981-09-28 00:00:00
abstract：:The purposes of this research were to quantify gravity receptor function in inbred mouse strains and compare vestibular and auditory function for strain- and age-matched animals. Vestibular evoked potentials (VsEPs) were collected for 19 inbred strains at ages from 35 to 389 days old. On average, C57BL/6J (35 to 190 d...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2006.01.066
更新日期：2006-05-26 00:00:00
abstract：:There is evidence that D1 and D2 dopamine receptor subtypes coexist at the cellular level in the striatum and act synergistically to mediate the effects of dopamine. Other data suggest that these receptor subtypes are largely segregated in different striatal projection pathways. We used in situ hybridization in serial...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(93)90303-5
更新日期：1993-09-03 00:00:00
abstract：:The distribution of neurocalcin-immunoreactive (NC-ir) primary sensory neurons was examined in the trigeminal ganglion (TG), mesencephalic trigeminal tract nucleus (Mes5) and intraoral structures. NC-ir primary sensory neurons were located in the TG but not the Mes5. The coexpression study demonstrated that virtually ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(00)02175-2
更新日期：2000-05-02 00:00:00
abstract：:This study shows that the axons of some raphespinal sympathoinhibitory neurons projecting to the third thoracic spinal segment emit branches in the third-fourth cervical spinal segments of cats. This was demonstrated by using time-controlled collision of neuronal action potentials initiated by stimuli applied at these...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(88)91417-5
更新日期：1988-02-16 00:00:00
abstract：:An apparent binding activity of [3H]glutathione was examined by using synaptic membrane preparations of the rat brain. The activity was found to be more than two times as high at 30 degrees C as that found at 2 degrees C. At 2 degrees C, the apparent binding sites consisted of a single component with a Kd of 0.77 micr...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(88)90524-0
更新日期：1988-10-25 00:00:00
abstract：:Development of noradrenergic responses in the rat dorsal motor nucleus of the vagus (DMV) from postnatal days 0 to 22 was studied by a conventional microelectrode technique using brain slice preparations. Between postnatal days 0 and 6, noradrenaline (NA) caused only alpha 1-adrenoceptor-mediated depolarizations. By p...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(89)91170-0
更新日期：1989-07-31 00:00:00
abstract：:Spatial neglect may involve neglect dyslexia resulting in a failure to identify words or parts of words in the contralesional hemispace. The present study explored a well-documented dissociation in neglect dyslexics' word processing, i.e. impaired reading aloud in the presence of good lexical decision performance for ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2007.10.069
更新日期：2008-01-16 00:00:00
