Abstract:
:Following the procedure of Jeffcoate et al. [1974] we have successfully obtained a specific antiserum to LH-RH in rabbits, and are utilizing our antibody to study the synthesis of LH-RH by rat hypothalamic tissues in vitro. Our antibody, used at a dilution of 1:20,000, binds 37% of added 125I-labelled LH-RH, and cross-reacts minimally with thyrotropin releasing factor (TRF; 4.0%), somatotropin release inhibiting factor (SRIF; 0.035%), and melanocyte inhibiting factor (MIF; 0.009%). The antiserum recovers LH-RH added to charcoal-stripped rat serum quantitatively. Serial dilutions of rat hypothalamic extract and varying aliquots of normal rat serum are parallel to the standard curve (synthetic LH-RH: 0-100 pg/tube). Hypothalamic tissue of 4 male rats, bounded by the optic chiasm, mammillary bodies and hypothalamic fissures to a depth of 2-3 mm, was pooled, minced and incubated for 1-3 h in 2 ml of Eagle's Minimum Essential Medium in atmosphere of 95% O2/5% CO2 with 10 muCi of 3H-glycine. Reactions were stopped by the addition of 1 ml 0.1 N HC1. The tissue and medium were homogenized together and boiled for 3 min. Aliquots from the incubates were neutralized, LH-RH levels measured by radioimmunoassay (RIA), and 3H-glycine incorporation determined by immunoprecipitation, using our antibody. 3H-glycine incorporation into presumptive LH-RH increased linearly over the 3 h period. Aliquots of the 3 h incubates were chromatographed on Sephadex G-25 columns (1 x 10 cm) using 0.01 M acetic acid for elution; 1 ml fractions were collected. Synthetic LH-RH was chromatographed in a similar manner, and its elution profile determined by UV absorbance at 280 mm. Aliquots of each fraction of the eluted material from the 3 h incubates were counted in a scintillation counter to determine the elution pattern of the labelled material. Additionally, aliquots from the 1 ml fractions were neutralized and used for immunoprecipitation. Coincident peaks of 3H-label, immunoprecipitable 3H-labelled presumptive LH-RH, and synthetic LH-RH were observed. These results lend strong evidence to support our conclusion that the technique of immunoprecipitation is an efficacious approach to the study of the synthesis of LH-RH by rat hypothalamic tissue in vitro.
journal_name
Neuroendocrinologyjournal_title
Neuroendocrinologyauthors
Hall RW,Steinberger Edoi
10.1159/000122517subject
Has Abstractpub_date
1976-01-01 00:00:00pages
111-9issue
2eissn
0028-3835issn
1423-0194journal_volume
21pub_type
杂志文章abstract::The relationship between concentrations of vasopressin in plasma and cerebrospinal fluid (CSF) was examined under basal and hypoxic conditions in unanesthetized and unrestrained adult female sheep. Under basal conditions mean (+/- SE) concentration of vasopressin (4.1 +/- 0.3 pg/ml) in CSF was significantly (p less th...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000123933
更新日期:1984-06-01 00:00:00
abstract::Ghrelin is a 28-amino-acid gastric peptide that potently stimulates growth hormone (GH) secretion in vivo and in vitro. Ghrelin-expressing cells have been found in the oxyntic region of the stomach and in the arcuate nucleus of the hypothalamus. The aim of this work was to investigate the regional distribution and dev...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000068653
更新日期:2003-02-01 00:00:00
abstract::The effect of long-term decreases in circulating concentrations of prolactin was determined on the responsiveness of tuberoinfundibular dopamine (DA) neurons to this hormone. The activity of these neurons in ovariectomized rats was estimated by measuring the rate of DA synthesis (DOPA accumulation after the administra...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000124100
更新日期:1985-05-01 00:00:00
abstract::Three control point enzymes of the Embden-Meyerhof pathway, hexokinase (HK), phosphofructokinase (PFK) and pyruvate kinase (PK) were measured by quantitative histochemical methods in individual hypothalamic nuclei of adult neonatally androgenized female rats. HK activity was significantly increased in anterior hypotha...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000122715
更新日期:1977-01-01 00:00:00
abstract::The expression of the alpha-subunit of the glycoprotein hormones was studied in the developing rat pituitary by in situ hybridization and immunocytochemical techniques. The alpha-subunit mRNA labelling was detected in Rathke's pouch at 12 days' gestation (E12); at E13, it was exclusively concentrated in the pars tuber...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000126600
更新日期:1993-12-01 00:00:00
abstract::The present studies were undertaken to investigate the effect of gender on thyrotropin-releasing hormone (TRH) gene expression in cultured anterior pituitary (AP) cells. AP cells derived from 15-day-old male, female, or female pups that had been neonatally treated with testosterone propionate (TP), were cultured for u...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000126815
更新日期:1995-01-01 00:00:00
abstract:INTRODUCTION:Several studies have suggested that diet, especially one enriched in microbiota-fermented fibers or fat, regulates behavior. The underlying mechanisms are currently unknown. We previously reported that certain macronutrients (fermentable fiber and protein) regulate energy homeostasis via the activation of ...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000514289
更新日期:2021-01-11 00:00:00
abstract::We have previously shown that the serotonergic regulation of renin secretion from the kidneys is mediated by a renin-releasing factor (RRF) that is present in both plasma and hypothalamus. The present studies were designed to determine the distribution of RRF in the brain and peripheral tissues and to test whether RRF...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000126170
更新日期:1992-05-01 00:00:00
abstract::Bromocriptine or other dopamine agonists are usually effective for the treatment of prolactin-secreting adenomas. Five to 18% of prolactinomas, however, do not respond to such therapy. We have shown previously that such resistance to bromocriptine correlates with reduced binding to the D2 receptor subtype of dopamine,...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000126764
更新日期:1994-09-01 00:00:00
abstract::Serotonin, a biologically active amine, is related to carcinoid syndrome in functioning neuroendocrine tumors (NETs). Telotristat ethyl is a novel inhibitor of the tryptophan hydroxylase (TPH), a key enzyme in the production of serotonin. While its use in patients with carcinoid syndrome and uncontrolled diarrhea unde...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000502200
更新日期:2020-01-01 00:00:00
abstract::In human African trypanosomiasis (sleeping sickness), sleep and wake episodes are sporadically distributed throughout the day and the night. Plasma melatonin, sleep-wakefulness and rectal temperature rhythms were studied in 9 Congolese patients suffering from sleeping sickness compared to 6 healthy controls submitted ...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000054351
更新日期:1998-07-01 00:00:00
abstract::We have examined the spinal cord for the presence of glucocorticoid-binding sites. For this purpose, cytosol from the spinal cord of adrenalectomized rats was incubated with (3H)-dexamethasone. Maximal binding was obtained after 20 h of incubation at 0 degree C in the presence of 20 mM molybdate, whereas at 20 degrees...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000124134
更新日期:1985-03-01 00:00:00
abstract::Circadian rhythms occur in hypothalamic serotonin content in the turkey. Inhibition of serotonin (5-HT) synthesis by parachlorophenylalanine (PCPA) blocks circadian variation in hypothalamic serotonin levels suggesting that the major cause of diurnal fluctuations is differing rates of synthesis of the amine. PCPA trea...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000123299
更新日期:1982-03-01 00:00:00
abstract:BACKGROUND:In 2014, the World Health Organization (WHO) released a classification system introducing neuroendocrine neoplasms (NENs) of the female reproductive tract, excluding the ovaries. This study aimed to evaluate whether retrospective adaption of the gastroenteropancreatic (GEP)-NEN classification is feasible for...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000506743
更新日期:2020-02-25 00:00:00
abstract::In the course of the search for the prolactin-releasing factor (PRF), we noticed that the posterior pituitary contained strong PRF activity and subsequently traced this activity to that of bovine neurophysin-II (NP-II). NP-II prepared in our lab was judged to be a homogeneous preparation according to Sephadex G-75 gel...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000124190
更新日期:1985-10-01 00:00:00
abstract::Prolactin release from primary cultured pituitary cells was investigated using a dynamic perifusion system. Although epinephrine (1 mumol/l) produced an elevation in the mean value of the prolactin concentration at the onset of the perifusion, the elevation was not statistically significant, and the overall effect of ...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000126424
更新日期:1993-04-01 00:00:00
abstract::It is not known where melatonin acts to influence the neuroendocrine axis of seasonally breeding mammals. However, since the pars tuberalis (PT) contains the highest density of melatonin receptors, this adenohypophyseal subdivision is a potential target. Gonadotropes are the only immunocytochemically detectable adenoh...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000127247
更新日期:1997-10-01 00:00:00
abstract::The effect of intravertebral infusion of angiotensin II on cardiac vagal efferent activity was studied in anesthetized mongrel dogs. Unilateral infusion of doses of angiotensin II as low as 5 ng/kg/min inhibited basal vagal tone and also decreased the slope of the relationship between cardiac vagal efferent activity a...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000124120
更新日期:1985-06-01 00:00:00
abstract::The neuropeptide galanin (GAL) is widely distributed in the central and peripheral nervous systems where it often coexists with catecholamines and acetylcholine. Recently we have reported that human GAL (hGAL) in man depresses the release of norepinephrine (NE) and the responses to both assumption of upright posture a...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000127143
更新日期:1996-11-01 00:00:00
abstract::Histamine (HA) stimulates the release of the pro-opiomelanocortin (POMC)-derived peptides ACTH, beta-endorphin (beta-END), beta-lipotropin and alpha-melanocyte-stimulating hormone, and HA is involved in the mediation of the stress-induced release of these peptides. The effect of HA is indirect and may involve the hypo...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000126258
更新日期:1992-09-01 00:00:00
abstract::The purpose of the present study was to evaluate the opioid receptor subtype mediating opioid modulation of growth hormone (GH) secretion during ontogeny. The mu-agonist morphine and the kappa agonist U50,488 caused a stimulation and inhibition of GH secretion, respectively, on postnatal day 10. Studies on postnatal d...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000127077
更新日期:1996-06-01 00:00:00
abstract::It is known that the neonatal treatment of rats with monosodium L-glutamate (MSG) induces several metabolic abnormalities, resulting in enhanced adiposity and hyperleptinemia. Our study was designed to explore the consequences of MSG-induced chronic hyperleptinemia on adrenal sensitivity to the inhibitory effect of ex...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000072799
更新日期:2003-09-01 00:00:00
abstract::In vivo single-unit extracellular recording was used to assess neuronal membrane sensitivity at the level of the midbrain central gray (MCG) to the iontophoresis of luteinizing hormone releasing hormone (LHRH) and beta-endorphin (beta-END). The percentage of change in firing rate was evaluated to determine the effect ...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000124792
更新日期:1987-06-01 00:00:00
abstract::The effect of the antidiarrheal drug loperamide, a mu-opiate agonist, on ACTH secretion and biosynthesis, cAMP generation and phosphoinositide turnover was studied in rat anterior pituitary cell cultures. The cAMP-dependent protein kinase A pathway was stimulated with both corticotropin-releasing hormone (CRH; 2-5 nM)...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000126466
更新日期:1993-06-01 00:00:00
abstract::alpha-Methyl-p-tyrosine (alpha-MT), a competitive inhibitor of tyrosine hydroxylase, was used to block the synthesis of hypothalamic catecholamines in immature female rats of 14, 16 and 30 days of age and in castrated adults. The administration of alpha-MT (300 mg/kg body weight, free base) induced a significant decay...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000125611
更新日期:1990-10-01 00:00:00
abstract::The sleep-wakefulness pattern and brain protein levels were determined in rats for 3 h following these conditions: administration of an inhibitor of protein synthesis (anisomycin), administration of several doses of rat growth hormone (GH) or thyrotropin, and administration of a combination of anisomycin + GH or thyro...
journal_title:Neuroendocrinology
pub_type: 杂志文章,评审
doi:10.1159/000122377
更新日期:1975-01-01 00:00:00
abstract::The effects of melatonin administration on thyroid physiology of female hamsters was investigated. A protocol of 25 micrograms given daily as subcutaneous injections late in the light period was found to inhibit blood levels of thyroxin (T4), triiodothyronine (T3) and thyrotropin (TSH). The free T4 index (FT4I) and th...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000123359
更新日期:1982-01-01 00:00:00
abstract::Distribution and properties of receptors for gonadotropin-releasing hormone (GnRH) were analyzed in the brain of adult male rats. Binding of the iodinated GnRH agonist Des-Gly10-(D-Ala6)-GnRH ethylamide was studied in hippocampus and anterior pituitary using three convergent approaches: quantitative autoradiography on...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000125053
更新日期:1988-11-01 00:00:00
abstract::Molecules of astrocyte origin influence gonadotropin-releasing hormone (GnRH) release and GnRH neuronal growth and differentiation. Furthermore, type 1 astrocytes express steroid receptors, presenting the possibility that steroid actions on GnRH neurons might occur via astrocytes. Utilizing GT1-7 cells, a GnRH-secreti...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000059434
更新日期:2002-06-01 00:00:00
abstract:UNLABELLED:Mediobasal hypothalamic slices of adult ovariectomized (OVX) rats treated or not with 17 beta-estradiol (E2) were superfused in buffered (pH 7.2) Locke medium containing bacitracin. A 6-min pulse of K+ (56 m M) was less effective in releasing luteinizing hormone releasing hormone (LH-RH) from mediobasal hypo...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000123883
更新日期:1984-02-01 00:00:00