Loperamide inhibits corticotrophic cell function by a naloxone-insensitive mechanism in the rat in vitro.

Abstract:

:The effect of the antidiarrheal drug loperamide, a mu-opiate agonist, on ACTH secretion and biosynthesis, cAMP generation and phosphoinositide turnover was studied in rat anterior pituitary cell cultures. The cAMP-dependent protein kinase A pathway was stimulated with both corticotropin-releasing hormone (CRH; 2-5 nM) and the membrane-permeable Bu(2)cAMP (0.5-2.5 mM). The protein kinase C pathway was stimulated with 1 microM arginine vasopressin (AVP) and 1-10 nM phorbol 12-myristate 13-acetate (PMA). After 3.5 h, loperamide (10 microM) had no effect on basal ACTH levels but significantly suppressed CRH-induced ACTH release, in a dose-dependent manner, to 60 +/- 4% of control (100%) (p < 0.0001). After 24 h, basal proopiomelanocortin mRNA was significantly decreased to 50% of control by loperamide (p < 0.05). The suppressive effect of loperamide on CRH-induced ACTH secretion was not reversible by naloxone (0.1-1,000 microM). Morphine (0.01-10 microM) had no effect on basal and CRH-induced ACTH secretion. Loperamide did not influence basal and CRH-induced adenylate cyclase activity in anterior pituitary cell membrane preparations, but it significantly blunted Bu(2)cAMP-induced ACTH secretion in cell culture from 100 +/- 4 to 77 +/- 4% (p < 0.05). In Ca(2+)-depleted medium (Ca2+ < 0.1 mM), loperamide had no suppressive effect on CRH-induced ACTH secretion. AVP-induced ACTH secretion was significantly suppressed by loperamide from 100 +/- 5 to 74 +/- 3% (p < 0.0001), while basal and AVP-induced inositol 1-phosphate generation and PMA-induced ACTH secretion were not affected by loperamide.(ABSTRACT TRUNCATED AT 250 WORDS)

journal_name

Neuroendocrinology

journal_title

Neuroendocrinology

authors

Auernhammer CJ,Renner U,Müller OA,Stalla J,Stalla GK

doi

10.1159/000126466

subject

Has Abstract

pub_date

1993-06-01 00:00:00

pages

1019-27

issue

6

eissn

0028-3835

issn

1423-0194

journal_volume

57

pub_type

杂志文章
  • Effects of heterologous growth hormones on hypothalamic and pituitary function in transgenic mice.

    abstract::The expression of the mouse metallothionein-I (mMT) promoter/human growth hormone (hGH) fusion gene in transgenic mice leads to female sterility and major alterations in the function of the hypothalamic-adenohypophyseal system. These alterations include increases in median-eminence norepinephrine turnover and plasma L...

    journal_title:Neuroendocrinology

    pub_type: 杂志文章

    doi:10.1159/000125743

    authors: Steger RW,Bartke A,Parkening TA,Collins T,Buonomo FC,Tang KC,Wagner TE,Yun JS

    更新日期:1991-04-01 00:00:00

  • Evidence of vasopressin in adenohypophysis: research into its role in corticotrope activity.

    abstract::The presence of vasopressin (VP) in pars distalis of rats and pigs was investigated. Using radioimmunoassay and bioassay of VP, a substance with immunological and biological properties of this hormone was found. This substance was not detected in the adenohypophysis of rats with diabetes insipidus. A partial purificat...

    journal_title:Neuroendocrinology

    pub_type: 杂志文章

    doi:10.1159/000122841

    authors: Chateau M,Marchetti J,Burlet A,Boulange M

    更新日期:1979-01-01 00:00:00

  • DIETARY FIBERS AND PROTEINS MODULATE BEHAVIOR VIA THE ACTIVATION OF INTESTINAL GLUCONEOGENESIS.

    abstract:INTRODUCTION:Several studies have suggested that diet, especially one enriched in microbiota-fermented fibers or fat, regulates behavior. The underlying mechanisms are currently unknown. We previously reported that certain macronutrients (fermentable fiber and protein) regulate energy homeostasis via the activation of ...

    journal_title:Neuroendocrinology

    pub_type: 杂志文章

    doi:10.1159/000514289

    authors: Sinet F,Soty M,Zemdegs J,Guiard B,Estrada J,Malleret G,Silva M,Mithieux G,Gautier-Stein A

    更新日期:2021-01-11 00:00:00

  • Dexamethasone inhibits corticotropin-releasing factor gene expression in the rat paraventricular nucleus.

    abstract::The effect of glucocorticoids on corticotropin-releasing factor (CRF) gene expression was studied by combination of in situ hybridization histochemistry and steroid implantation. Dexamethasone micropellets, implanted around the hypothalamic paraventricular nucleus (PVN), caused total inhibition of the hybridizable CRF...

    journal_title:Neuroendocrinology

    pub_type: 杂志文章

    doi:10.1159/000124846

    authors: Kovács KJ,Mezey E

    更新日期:1987-10-01 00:00:00

  • Hyperprolactinemia induced by pituitary isografts suppresses the priming effect of LH-releasing hormone in normal and hypogonadal mice.

    abstract::We have investigated the effects of hyperprolactinemia, produced by pituitary isografts under the kidney capsule (16-20 days), on the LH releasing action and priming effect of LH-releasing hormone (LHRH) in normal and hypogonadal (hpg) female mice. The pituitary grafts increased the plasma prolactin concentrations abo...

    journal_title:Neuroendocrinology

    pub_type: 杂志文章

    doi:10.1159/000124585

    authors: Lewis CE,Fink G,Dow RC,Morris JF

    更新日期:1986-01-01 00:00:00

  • Comparison of chemotherapeutic treatment outcomes of advanced extrapulmonary neuroendocrine carcinomas and advanced small-cell lung carcinoma.

    abstract:BACKGROUND:The chemotherapy for small-cell lung carcinoma (SCLC) has been adopted for advanced extrapulmonary neuroendocrine carcinomas (EP-NECs). The aim of this study was to clarify the efficacy of standard SCLC regimens when used to treat EP-NECs and to compare the outcome with that for SCLC. METHODS:We reviewed th...

    journal_title:Neuroendocrinology

    pub_type: 杂志文章

    doi:10.1159/000338794

    authors: Terashima T,Morizane C,Hiraoka N,Tsuda H,Tamura T,Shimada Y,Kaneko S,Kushima R,Ueno H,Kondo S,Ikeda M,Okusaka T

    更新日期:2012-01-01 00:00:00

  • Inhibition by prolactin of post-castration rise in LH.

    abstract::Castration of male and female rats resulted in a marked rise in serum LH. The rise in serum LH was partially or completely prevented by injection of prolactin (Prl), by implantation of a small amount of Prl in the median eminence (ME), by grafting 2 anterior pituitaries (APs) underneath the kidney capsule, or by trans...

    journal_title:Neuroendocrinology

    pub_type: 杂志文章

    doi:10.1159/000122679

    authors: Grandison L,Hodson C,Chen HT,Advis J,Simpkins J,Meites J

    更新日期:1977-01-01 00:00:00

  • Regulation of hypothalamic proopiomelanocortin by leptin in lean and obese rats.

    abstract::The mechanisms by which leptin influences energy homeostasis are not entirely understood. Several observations indicate that proopiomelanocortin (POMC) is involved in the regulation of food intake and may be a mediator of leptin action. To further study this interaction, a sensitive solution hybridization assay was us...

    journal_title:Neuroendocrinology

    pub_type: 杂志文章

    doi:10.1159/000054499

    authors: Korner J,Chua SC Jr,Williams JA,Leibel RL,Wardlaw SL

    更新日期:1999-12-01 00:00:00

  • Increased tumor cell multiplication after radiofrequency lesions in median hypothalamus in the mouse and rat.

    abstract::A significant increase of cell multiplication in inoculated ascitic and solid tumors was demonstrated in both DBA/2 and C57BL/6 mice as well as in Wistar rats after radiofrequency lesions in the median hypothalamus (ventromedial and dorsomedial nuclei; part of arcuate nucleus). The following tests were performed: mito...

    journal_title:Neuroendocrinology

    pub_type: 杂志文章

    doi:10.1159/000124479

    authors: Bindoni M,Belluardo N,Marchese AE,Cardile V,Mudò G,Cella S,Laguidara A,Denatale G

    更新日期:1986-01-01 00:00:00

  • Effects of carbohydrate-active steroids and ACTH on visually-evoked responses in patients with adrenal cortical insufficiency.

    abstract::Visual average evoked responses (AERs) to 4 intensities of light were studied in 5 patients with adrenal cortical insufficiency. Patients were tested off all medications, on ACTH while off all medications, and during replacement with carbohydrate-active steroids. Off treatment, when steroid levels were low, AERs were ...

    journal_title:Neuroendocrinology

    pub_type: 杂志文章

    doi:10.1159/000122452

    authors: Buchsbaum MS,Henkin RI

    更新日期:1975-01-01 00:00:00

  • Regulatory activity and topological distribution of folliculo-stellate cells in rat anterior pituitary cell aggregates.

    abstract::An enriched population of cells immunoreactive to antiserum against S-100 protein, a marker of folliculo-stellate (FS) cells in the rat pituitary, was obtained by separation of dispersed pituitary cells from adult female rats by gradient sedimentation at unit gravity. The effect of FS cells on the stimulation and inhi...

    journal_title:Neuroendocrinology

    pub_type: 杂志文章

    doi:10.1159/000125146

    authors: Allaerts W,Denef C

    更新日期:1989-04-01 00:00:00

  • Effects of food deprivation on the GH axis: immunocytochemical and molecular analysis.

    abstract::Neuroendocrine mechanisms governing growth hormone (GH) secretion are sensitive to nutritional status since the normal pulsatile pattern of GH release is disrupted during conditions of food deprivation or malnutrition. A reasonable hypothesis for this occurrence is the alteration of somatostatin and GH-releasing hormo...

    journal_title:Neuroendocrinology

    pub_type: 杂志文章

    doi:10.1159/000127172

    authors: Brogan RS,Fife SK,Conley LK,Giustina A,Wehrenberg WB

    更新日期:1997-02-01 00:00:00

  • Infundibular gonadotropin-releasing hormone neurons are inhibited by direct opioid and autoregulatory synapses in juvenile monkeys.

    abstract::A consistent group of gonadotropin-releasing hormone (GnRH) cell bodies occurs in the ventral hypothalamic tract at the infundibular lip (IL), just below the arcuate nucleus (ARC), at the site of the so-called GnRH 'pulse generator'. Immunocytochemical studies were performed to examine contacts between these GnRH neur...

    journal_title:Neuroendocrinology

    pub_type: 杂志文章

    doi:10.1159/000124914

    authors: Thind KK,Goldsmith PC

    更新日期:1988-03-01 00:00:00

  • Beta-adrenergic receptors in the premammillary nucleus mediate the inhibition of LH release evoked by locus ceruleus stimulation.

    abstract::The neurotransmitter and andrenoceptors involved in the inhibition of luteinizing hormone (LH) release induced by stimulating the locus ceruleus nucleus (LC) have been characterized in ovariectomized, estrogen-primed rats in which the release of LH was triggered by electrical stimulation of the medial preoptic area. B...

    journal_title:Neuroendocrinology

    pub_type: 杂志文章

    doi:10.1159/000123858

    authors: Dotti C,Taleisnik S

    更新日期:1984-01-01 00:00:00

  • Distribution of a renin-releasing factor in the central nervous system of the rat.

    abstract::We have previously shown that the serotonergic regulation of renin secretion from the kidneys is mediated by a renin-releasing factor (RRF) that is present in both plasma and hypothalamus. The present studies were designed to determine the distribution of RRF in the brain and peripheral tissues and to test whether RRF...

    journal_title:Neuroendocrinology

    pub_type: 杂志文章

    doi:10.1159/000126170

    authors: Urban JH,Brownfield MS,Levine JE,Van de Kar LD

    更新日期:1992-05-01 00:00:00

  • Effects of somatostatin on human satiety.

    abstract::Somatostatin (ST) inhibits gastrointestinal motility and exocrine and endocrine secretions. In animals, ST has been demonstrated to decrease food intake. We investigated, in a randomized double-blind investigation in 10 healthy humans, the effects of an intravenous ST infusion compared to saline on subjective hunger f...

    journal_title:Neuroendocrinology

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1159/000126831

    authors: Lieverse RJ,Jansen JB,Masclee AM,Lamers CB

    更新日期:1995-02-01 00:00:00

  • Activation of masculine sexual behavior by intracranial estradiol benzoate implants in male rats.

    abstract::Intracranial implants of estradiol benzoate (EB) were used to explore sites of action for the activation of masculine copulatory behavior in castrated male rats. In experiment 1, 27 or 30 gauge implants were placed unilaterally throughout the hypothalamus and preoptic area. Dihydrotestosterone (DHT) was administered s...

    journal_title:Neuroendocrinology

    pub_type: 杂志文章

    doi:10.1159/000122865

    authors: Davis PG,Barfield RJ

    更新日期:1979-01-01 00:00:00

  • Hypothalamic infusion of activin A increases water consumption and urine volume in the rat.

    abstract::Activin A immunoreactivity has been localized within the nucleus tractus solitarius and its projections into regions of the hypothalamus such as the paraventricular nucleus. However, the function of activin within the central nervous system is unclear. Because these regions of the brain are involved in the regulation ...

    journal_title:Neuroendocrinology

    pub_type: 杂志文章

    doi:10.1159/000126399

    authors: Schwall RH,Jakeman LB,Altar CA

    更新日期:1993-03-01 00:00:00

  • New insights into the role of chronic inflammation and cytokines in the etiopathogenesis of gastroenteropancreatic neuroendocrine tumors.

    abstract::Although previously considered rare, recent epidemiological studies have revealed that the incidence (3.6/100,000) and prevalence (35/100,000) of gastroenteropancreatic neuroendocrine tumors (GEP-NETs) has increased over the past few decades. Despite the progress in the understanding of GEP-NET molecular biology, ther...

    journal_title:Neuroendocrinology

    pub_type: 杂志文章,评审

    doi:10.1159/000362339

    authors: Cigrovski Berkovic M,Cacev T,Catela Ivkovic T,Zjacic-Rotkvic V,Kapitanovic S

    更新日期:2014-01-01 00:00:00

  • Effects of sex steroid hormones on the expression of somatostatin receptors sst1 and sst5 in goldfish pituitary and forebrain.

    abstract::In the present paper the effects of estradiol and testosterone on the expression of the types 1 and 5 somatostatin receptors (sst1 and sst5) in the goldfish forebrain and pituitary were investigated. Estradiol increased the sst1 expression in both the forebrain and pituitary in a dose- and time-dependent manner. In ad...

    journal_title:Neuroendocrinology

    pub_type: 杂志文章

    doi:10.1159/000071963

    authors: Canosa LF,Lin X,Peter RE

    更新日期:2003-08-01 00:00:00

  • Monoclonal antibodies against the human somatostatin receptor subtypes 1-5: development and immunohistochemical application in neuroendocrine tumors.

    abstract:BACKGROUND:Activation of somatostatin receptors (sstr1-5) by somatostatin and its analogues exerts an inhibitory effect on hormone secretion and provides the basis for the treatment of a range of endocrine diseases such as acromegaly, Cushing's disease and neuroendocrine tumors (NET). The lack of well-characterized com...

    journal_title:Neuroendocrinology

    pub_type: 杂志文章

    doi:10.1159/000330616

    authors: Schmid HA,Lambertini C,van Vugt HH,Barzaghi-Rinaudo P,Schäfer J,Hillenbrand R,Sailer AW,Kaufmann M,Nuciforo P

    更新日期:2012-01-01 00:00:00

  • Beta-endorphin regulation of LHRH release at the median eminence level: immunocytochemical and physiological evidence in hens.

    abstract::We studied the effect that beta-endorphin (beta END) might have at the median eminence (ME) on luteinizing hormone (LH)-releasing hormone (LHRH) during the ovulatory cycle of domestic hens. Thus, we assessed (a) the immunocytochemical distribution of beta END and LHRH in the hen ME, (b) the temporal changes in ME and ...

    journal_title:Neuroendocrinology

    pub_type: 杂志文章

    doi:10.1159/000126381

    authors: Contijoch AM,Malamed S,Sarkar DK,Advis JP

    更新日期:1993-01-01 00:00:00

  • Fetal development of delta-sleep-inducing-peptide-like immunoreactivity in hypothalamus of guinea pig with special regard to the prenatal colocalization with gonadotropin-releasing-hormone-like immunoreactivity.

    abstract::Delta-sleep-inducing peptide (DSIP) colocalizes within gonadotropin-releasing-hormone (GnRH)-containing neurons in adult hypothalamus and could play a role in the regulation of hypothalamic-pituitary axis in adults. To support the possibility that DSIP also participates in fetal neuroendocrine events and to demonstrat...

    journal_title:Neuroendocrinology

    pub_type: 杂志文章

    doi:10.1159/000126098

    authors: Pu LP,Dubois PM

    更新日期:1992-01-01 00:00:00

  • Unifocal versus multiple ileal neuroendocrine tumors location: an embryological origin.

    abstract:INTRODUCTION:Small-intestinal neuroendocrine tumors (SI-NET) are situated preferentially within the ileum. The aim was to describe a potential difference in location between unifocal and multiple ileal-NET. METHODS:Between December 2010 and December 2019, all consecutive patients who underwent resection in our Europea...

    journal_title:Neuroendocrinology

    pub_type: 杂志文章

    doi:10.1159/000511849

    authors: Kalifi M,Walter T,Milot L,Hervieu V,Millot I,Gibert B,Roche C,Forestier J,Lombard-Bohas C,Pasquer A,Poncet G

    更新日期:2020-09-30 00:00:00

  • Androgen and estrogen receptors coexist within individual neurons in the brain of the Syrian hamster.

    abstract::Many aspects of reproductive neuroendocrine function and sexual behavior are responsive to both androgens and estrogens, suggesting that receptors for these steroid hormones may reside within single cells in brain regions that control reproductive function. We determined the distribution of estrogen receptor-containin...

    journal_title:Neuroendocrinology

    pub_type: 杂志文章

    doi:10.1159/000127039

    authors: Wood RI,Newman SW

    更新日期:1995-11-01 00:00:00

  • Septal and hippocampal release of vasopressin and oxytocin during late pregnancy and parturition in the rat.

    abstract::The push-pull perfusion technique, in conjunction with specific radioimmunoassays, was used to monitor the release of both arginine vasopressin (AVP) and oxytocin (OXT) within distinct limbic brain areas of conscious female rats. In pregnant rats near term, the release of AVP was greater than that of virgin rats in bo...

    journal_title:Neuroendocrinology

    pub_type: 杂志文章

    doi:10.1159/000125917

    authors: Landgraf R,Neumann I,Pittman QJ

    更新日期:1991-10-01 00:00:00

  • Stimulatory versus inhibitory effects of progesterone on estrogen-induced phasic LH and prolactin secretion correlated with estrogen nuclear and progestin cytosol receptor concentrations in brain and pituitary gland.

    abstract::The purpose of these studies was to determine the temporal requirements and time of day required for progesterone (P4) to amplify versus extinguish LH surges. The experimental animal model used in these studies was the 7-day ovariectomized rat (day 0) treated continuously with estradiol (E2) for 2-4 days (days 2, 3 or...

    journal_title:Neuroendocrinology

    pub_type: 杂志文章

    doi:10.1159/000124241

    authors: Barraclough CA,Camp P,Weiland N,Akabori A

    更新日期:1986-01-01 00:00:00

  • ADH release from cut pituitary stalk and intact pituitary gland during amygdala stimulation at various frequencies in rats.

    abstract::Changes in antidiuretic substance (i.e., vasopressin) release from the cut pituitary stalk and the intact pituitary gland were observed during electrical stimulation of amygdala medial nucleus. Electrical pulses of 4 or 36 cycles per second (cps) were used and in both cases the same energy (about 2.160 nanowatt second...

    journal_title:Neuroendocrinology

    pub_type: 杂志文章

    doi:10.1159/000122349

    authors: Tomas T

    更新日期:1975-01-01 00:00:00

  • Progesterone, but not LHRH or prostaglandin E2, induces sequential inhibition of lordosis to various lordogenic agents.

    abstract::In experiment I we studied the capacity of progesterone (P) and two nonsteroidal agents that activate lordosis, but do not bind to the progestin receptor (PR), i.e. luteinizing hormone-releasing hormone (LHRH) and prostaglandin E2 (PGE2) to induce sequential inhibition (SI) in ovariectomized estradiol-primed rats. The...

    journal_title:Neuroendocrinology

    pub_type: 杂志文章

    doi:10.1159/000126457

    authors: González-Mariscal G,Melo AI,Beyer C

    更新日期:1993-05-01 00:00:00

  • Site-specific induction of Fos immunoreactivity in preoptic and hypothalamic NADPH-positive neurons during glucoprivation.

    abstract::Neuronal nitric oxide synthase, e.g. NADPH diaphorase (NADPH-d), catalyzes formation of the free radical, nitric oxide (NO), and occurs within brain structures that have functional significance for energy fuel homeostasis. The following studies examined whether populations of NADPH-d-positive neurons in the hypothalam...

    journal_title:Neuroendocrinology

    pub_type: 杂志文章

    doi:10.1159/000054417

    authors: Briski KP,Sylvester PW

    更新日期:1999-03-01 00:00:00