Abstract：
UNLABELLED:Mediobasal hypothalamic slices of adult ovariectomized (OVX) rats treated or not with 17 beta-estradiol (E2) were superfused in buffered (pH 7.2) Locke medium containing bacitracin. A 6-min pulse of K+ (56 m M) was less effective in releasing luteinizing hormone releasing hormone (LH-RH) from mediobasal hypothalamic slices sampled from OVX rats than from OVX animals treated subcutaneously with either E2 or stilbestrol implants for 5 days; in contrast, the basal release of the neuropeptide was identical in both cases. Direct addition to the superfusion medium of 17 beta-estradiol (10(-10) to 10(-7) M) or stilbestrol (10(-8) M) potentiated the K+-induced LH-RH release from slices of OVX animals. The K+-induced LH-RH release observed after in vivo E2 implantation was not further amplified by in vitro addition of the hormone. Tamoxifen and hydroxytamoxifen, estrogen antagonists, were ineffective by themselves, but reversed the E2 facilitation of K+-evoked LH-RH release. In contrast, 17 alpha-estradiol, progesterone, or cholesterol (10(-8) or 10(-9) M) hat no effect on either basal or stimulated release of the neurohormone. Somatostatin release measured under identical conditions was not affected by castration or by in vitro addition of the steroid. IN CONCLUSION:(1) estradiol appears selectively and specifically involved in the process coupling, nerve endings depolarization, and LH-RH release, and (2) the effect is receptor-mediated and does not appear to require nuclear translocation of the steroid or transcription processes, since it can be readily elicited upon addition of the hormone to nerve endings disconnected from their cell bodies.
journal_name
Neuroendocrinologyjournal_title
Neuroendocrinologyauthors
Drouva SV,Laplante E,Gautron JP,Kordon Cdoi
10.1159/000123883subject
Has Abstractpub_date
1984-02-01 00:00:00pages
152-7issue
2eissn
0028-3835issn
1423-0194journal_volume
38pub_type
杂志文章abstract：:Recent evidence suggests that the free amino-terminal fragment of procalcitonin (N-PCT) plays a role in the central control of feeding behavior and energy homeostasis. However, little is known about the mechanisms through which N-PCT works. Here we report that intracerebroventricular administration of N-PCT to free-fe...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000155137
更新日期：2008-01-01 00:00:00
abstract：:The concentrations in plasma of the biologically active endogenous peptide Tyr-MIF-1 (Tyr-Pro-Leu-Gly-NH2) have not been measured during development or in female rats. By radioimmunoassay, we found that Tyr-MIF-1-like immunoreactivity (Tyr-MIF-1-LI) was first consistently detectable in plasma when the rat was 5 days o...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000126322
更新日期：1992-12-01 00:00:00
abstract：:Neurons in the adult rat ventromedial hypothalamic nucleus (VMN, 4-6 neurons per brain; 3-7 brains per group) were studied under various hormonal conditions using the single-section Golgi impregnation technique. Intact rats of both sexes and ovariectomized females treated with oil, estrogen or estrogen and progesteron...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000125387
更新日期：1990-05-01 00:00:00
abstract：:The c-fos protein is rapidly induced in hypothalamic magnocellular nuclei following hemorrhage. We used specific antibodies directed against c-fos and either vasopressin (AVP) or oxytocin (OT) neurophysin to investigate c-fos activation in individual AVP and OT neurons. AVP and OT neurons expressed c-fos in response t...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000126384
更新日期：1993-03-01 00:00:00
abstract：:The hypophysial portal vessels and anterior pituitary glands of adult male rats were surgically exposed, cannulated and infused for 1 min with saline, growth hormone-releasing factor (GH-RF), and dbcAMP. After cessation of infusion, anterior pituitary glands were collected at 1, 5, 15, 30 or 60 min for electron micros...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000122421
更新日期：1975-01-01 00:00:00
abstract：:While interleukin-1 (IL-1), a monocyte-derived polypeptide, clearly stimulates the hypothalamo-pituitary-adrenal (HPA) axis, its precise site of action is controversial. In these studies, the possibility of a hypothalamic and/or a pituitary site of action was investigated in vitro, using incubated rat hypothalami and ...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000125096
更新日期：1989-01-01 00:00:00
abstract：:In human African trypanosomiasis (sleeping sickness), sleep and wake episodes are sporadically distributed throughout the day and the night. Plasma melatonin, sleep-wakefulness and rectal temperature rhythms were studied in 9 Congolese patients suffering from sleeping sickness compared to 6 healthy controls submitted ...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000054351
更新日期：1998-07-01 00:00:00
abstract：:Activin A immunoreactivity has been localized within the nucleus tractus solitarius and its projections into regions of the hypothalamus such as the paraventricular nucleus. However, the function of activin within the central nervous system is unclear. Because these regions of the brain are involved in the regulation ...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000126399
更新日期：1993-03-01 00:00:00
abstract：:We have previously reported that the somatotropin regulatory peptides, growth hormone-releasing hormone (GRF) and somatostatin (SS) increase feeding when applied centrally in low picomole-range doses. The similarity between the dose-response characteristics and photoperiod sensitivities of these two peptide's feeding ...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000126372
更新日期：1993-01-01 00:00:00
abstract：:Hypophysectomized male rats with adenohypophyses grafted under the kidney capsule (HG rat) for 3 weeks with or without estradiol implantation, were used to examine prolactin (PRL) secretion independent of hypothalamic influence. Changes in the circulating PRL concentration were monitored by taking blood samples every ...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000123146
更新日期：1981-03-01 00:00:00
abstract：:The anterior pituitary is permanently regulated by processes of apoptosis and proliferation in order to maintain tissue homeostasis. Several factors have been implicated in this regulation and lately, prolactin (PRL) has been included into that list. However, since PRL is secreted by anterior pituitary lactotropes, th...
journal_title：Neuroendocrinology
pub_type： 杂志文章,评审
doi：10.1159/000354701
更新日期：2013-01-01 00:00:00
abstract：:The effect of altering gamma-aminobutyric acid (GABA) activity on growth hormone (GH) secretion of freely moving chronically cannulated male rats was studied. Systemic injection of the GABA agonist muscimol (2 mg/kg i.v.) inhibited the anticipated secretory episode. Increasing brain GABA levels by gamma-acetylenic-GAB...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000124031
更新日期：1984-12-01 00:00:00
abstract：:Allopregnanolone is a neuroactive steroid present in the brain, but also measurable in systemic circulation. It exhibits anxiolytic and anticonvulsant effects and is able to produce hyperphagia. Since eating behavior disturbances and increased peripheral basal sympathetic activity have been reported in obese subjects,...
journal_title：Neuroendocrinology
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1159/000048228
更新日期：2002-02-01 00:00:00
abstract：:Previous work in many laboratories has shown that luteinizing hormone-releasing hormone (LHRH) neurons display a range of shapes from smooth-contoured to extremely irregular or 'thorny'. To determine if these forms reflect or can be modified by the gonadal steroid milieu, the morphology of LHRH neurons was examined in...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000125138
更新日期：1989-04-01 00:00:00
abstract：:Serotonin, a biologically active amine, is related to carcinoid syndrome in functioning neuroendocrine tumors (NETs). Telotristat ethyl is a novel inhibitor of the tryptophan hydroxylase (TPH), a key enzyme in the production of serotonin. While its use in patients with carcinoid syndrome and uncontrolled diarrhea unde...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000502200
更新日期：2020-01-01 00:00:00
abstract：:There is evidence that alpha-melanocyte-stimulating hormone (alpha-MSH) has immunomodulatory and anti-inflammatory actions within the brain. In this study, we tested whether these actions are due to inhibition of the synthesis of nitric oxide (NO) and prostaglandins induced by lipopolysaccharide (LPS). Since melanocor...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000079321
更新日期：2004-01-01 00:00:00
abstract：:The injection of purified alpha-bungarotoxin (alpha-BTX), an antagonist of the muscle nicotinic cholinergic receptors, into the third ventricle (3rd V) of ovariectomized rats decreased the frequency of pulses of luteinizing hormone (LH) but had no significant effect on the amplitude or nadir of LH secretion. Approxima...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000125153
更新日期：1989-05-01 00:00:00
abstract：BACKGROUND/AIM:The mode of action of the somatostatin analog octreotide on neuro-endocrine tumour proliferation is largely unknown. Overexpression of the proto-oncogene Akt/PKB (protein kinase B) has been demonstrated in certain neuro-endocrine tumours: Akt activates downstream proteins including mTOR and p70S6K, which...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000111501
更新日期：2008-01-01 00:00:00
abstract：:The effects of melatonin and an aqueous extract of bovine pineal glands on adrenal function were studied in pinealectomized, ovariectomized and hypophysectomized rats. Melatonin (50 microgram i.p., twice daily for 1 week) stimulates adrenal 5 alpha-reductase activity in intact animals and after ovariectomy. A correspo...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000122659
更新日期：1977-01-01 00:00:00
abstract：:Acute electrolytic stimulation of the median raphe (MR) nucleus (MR Stim) between 13:00 and 16:00 h on proestrus blocked ovulation in 10 of the 21 rats tested, whereas all sham-treated (MR Sham, no current) animals ovulated normally (n = 12). MR Stim at 16:00 h on proestrus in individual cannulated rats 'turned off' t...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000122884
更新日期：1979-01-01 00:00:00
abstract：:Molecules of astrocyte origin influence gonadotropin-releasing hormone (GnRH) release and GnRH neuronal growth and differentiation. Furthermore, type 1 astrocytes express steroid receptors, presenting the possibility that steroid actions on GnRH neurons might occur via astrocytes. Utilizing GT1-7 cells, a GnRH-secreti...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000059434
更新日期：2002-06-01 00:00:00
abstract：:Vasostatin, a fragment of calreticulin, was transfected in the BON cell line to evaluate the feasibility of using it for gene therapy in neuroendocrine tumors. Vasostatin transfected cells were subcutaneously inoculated in nude mice. Burkitt lymphoma cell line, CA46, colorectal adenocarcinoma cell line, SW480, as well...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000089749
更新日期：2005-01-01 00:00:00
abstract：:In the present study we demonstrated that TLQP-21, a biologically active peptide derived from the processing of the larger pro-VGF granin, plays a role in mammotrophic cell differentiation. We used an established in vitro model, the GH3 cell line, which upon treatment with epidermal growth factor develops a mammotroph...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000339855
更新日期：2013-01-01 00:00:00
abstract：:To determine whether the inhibitory effect of clonidine (CLON) on renin secretion is due in part to a direct action on the kidneys or due entirely to an action on the brain, the drug was administered intravenously in a dose of 30 microgram/kg to dogs in which the spinal cord had been transected in the cervical region....
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000122733
更新日期：1978-01-01 00:00:00
abstract：BACKGROUND:Kisspeptins are recognized as important stimulators of gonadotropin-releasing hormone (GnRH) and gonadotropin secretion. In sheep, the nonbreeding (anestrous) period is characterized by reduced Kiss1 mRNA expression in the hypothalamus. The aim of the present study was to determine whether the response to ki...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000227806
更新日期：2009-01-01 00:00:00
abstract：:Corticosteroids regulate gene expression through the activation of mineralocorticoid and glucocorticoid receptors. The hippocampus contains the highest density of mineralocorticoid and glucocorticoid receptors in the central nervous system. The modulation of neuron excitability by corticosteroids in hippocampal subfie...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000054299
更新日期：1998-01-01 00:00:00
abstract：:We have investigated the hypothesis that administration of progesterone to estrogen-treated ovariectomized (OVX) rats enhances the stimulatory effects of neuropeptide Y (NPY) on the secretion of luteinizing hormone-releasing hormone (LHRH) from median eminence (ME) fragments in vitro. Adult rats were bilaterally OVX a...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000125651
更新日期：1990-12-01 00:00:00
abstract：:We studied the effect that beta-endorphin (beta END) might have at the median eminence (ME) on luteinizing hormone (LH)-releasing hormone (LHRH) during the ovulatory cycle of domestic hens. Thus, we assessed (a) the immunocytochemical distribution of beta END and LHRH in the hen ME, (b) the temporal changes in ME and ...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000126381
更新日期：1993-01-01 00:00:00
abstract：:The present study was designed to investigate the role of glucocorticoids in mediating the stimulatory effect of interleukin-1 (IL-1) upon the adrenocortical (AC) axis. Intact male rats were injected with either vehicle or dexamethasone (DEX) (5-50 micrograms/100 g b.w.) and were subsequently exposed to ether stress o...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000125294
更新日期：1989-12-01 00:00:00
abstract：:An abolishment of 24 h periodicity of the adrenocortical activity was observed in rats subjected to a prolonged food deprivation of 7--8 days. Total locomotor activity progressively decreased and its circadian rhythm faded out in a similar fashion as the adrenocortical rhythm. The circadian rhythms of both locomotor a...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000122924
更新日期：1979-01-01 00:00:00
