Abstract：
:In the present study we demonstrated that TLQP-21, a biologically active peptide derived from the processing of the larger pro-VGF granin, plays a role in mammotrophic cell differentiation. We used an established in vitro model, the GH3 cell line, which upon treatment with epidermal growth factor develops a mammotrophic phenotype consisting of induction of prolactin expression and secretion, and inhibition of growth hormone. Here we determined for the first time that during mammotrophic differentiation, epidermal growth factor also induces Vgf gene expression and increases VGF protein precursor processing and peptide secretion. After this initial observation we set out to determine the specific role of the VGF encoded TLQP-21 peptide on this model. TLQP-21 induced a trophic effect on GH3 cells and increased prolactin expression and its own gene transcription without affecting growth hormone expression. TLQP-21 was also able to induce a significant rise of cytoplasmic calcium, as measured by Fura2AM, due to the release from a thapsigargin-sensitive store. TLQP-21-dependent rise in cytoplasmic calcium was, at least in part, dependent on the activation of phospholipase followed by phosphorylation of PKC and ERK. Taken together, the present results demonstrate that TLQP-21 contributes to differentiation of the GH3 cell line toward a mammotrophic phenotype and suggest that it may exert a neuroendocrine role in vivo on lactotroph cells in the pituitary gland.
journal_name
Neuroendocrinologyjournal_title
Neuroendocrinologyauthors
Petrocchi Passeri P,Biondini L,Mongiardi MP,Mordini N,Quaresima S,Frank C,Baratta M,Bartolomucci A,Levi A,Severini C,Possenti Rdoi
10.1159/000339855subject
Has Abstractpub_date
2013-01-01 00:00:00pages
212-24issue
3eissn
0028-3835issn
1423-0194pii
000339855journal_volume
97pub_type
杂志文章abstract：:In experiment I we studied the capacity of progesterone (P) and two nonsteroidal agents that activate lordosis, but do not bind to the progestin receptor (PR), i.e. luteinizing hormone-releasing hormone (LHRH) and prostaglandin E2 (PGE2) to induce sequential inhibition (SI) in ovariectomized estradiol-primed rats. The...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000126457
更新日期：1993-05-01 00:00:00
abstract：:The present study examined the effect of estradiol on hypothalamic serotonin-1A (5-HT(1A)) receptor signaling in female rats. We first examined the time-course effects of a single injection of the 5-HT(1A) receptor agonist (+/-)8-OH-DPAT (5, 15 or 30 min prior to decapitation), and dose response of (+)8-OH-DPAT (50, 1...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000080795
更新日期：2004-01-01 00:00:00
abstract：:The present study was designed to investigate whether the vasoactive intestinal peptide (VIP) concentration in hypothalamic nuclei, dorsal raphe nucleus (DR) and pituitary lobes of lactating rats changes in physiological situations when prolactin (PRL) secretion is stimulated (suckling) or inhibited (pup separation). ...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000125854
更新日期：1991-07-01 00:00:00
abstract：:Prolactin-releasing (PRF) activity was found in Pitressin (a commercial extract from posterior pituitary for vasopressin). Injection of Pitressin into conscious free-moving rats implanted with a permanent atrial indwelling cannula, produced a transient increase in prolactin concentration in the circulation. In order t...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000123277
更新日期：1982-01-01 00:00:00
abstract：:The role of endogenous opioid peptides in the control of gonadotropin-releasing hormone pulse generator activity was examined in long-term ovariectomized goats by recording the hypothalamic multiple-unit activity specifically associated with pulsatile luteinizing hormone secretion (MUA volleys). Administration of nalo...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000126418
更新日期：1993-04-01 00:00:00
abstract：:In the Djungarian hamster Phodopus sungorus, the daily temporal pattern of synthesis and release of pineal hormone melatonin, mainly the length of the period of elevated melatonin levels, may be involved in transferring the information on day length to the neuroendocrine-gonadal axis. The present study investigated th...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000123895
更新日期：1984-03-01 00:00:00
abstract：:The purpose of the present study was to evaluate the opioid receptor subtype mediating opioid modulation of growth hormone (GH) secretion during ontogeny. The mu-agonist morphine and the kappa agonist U50,488 caused a stimulation and inhibition of GH secretion, respectively, on postnatal day 10. Studies on postnatal d...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000127077
更新日期：1996-06-01 00:00:00
abstract：:Leptin exerts important effects on the regulation of food intake and energy expenditure by acting in the brain. Leptin action is mediated by the interaction with a receptor that is alternatively spliced, resulting in at least five different isoforms. The long form (OB-Rb) has a long intracellular domain that is essent...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000054536
更新日期：2000-03-01 00:00:00
abstract：:Endogenous gonadoliberin was localized by using immunocytological methods in the hypothalamic neurons which synthetize the peptide and in the hypophysial target cells. The rat tissues were fixed and frozen. Ultrathin sections were obtained by cryoultramicrotomy. In the pituitary gland, GnRH was observed in both gonado...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000123300
更新日期：1982-03-01 00:00:00
abstract：:It has been well documented that interleukin-1beta (IL-1beta) is a major mediator for recruiting the hypothalamo-pituitary-adrenal (HPA) axis following infectious disease. The recent localization of IL-1beta receptors in neurons of the hippocampus provides further support for the role of IL-1beta as a neurotransmitter...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000054405
更新日期：1999-02-01 00:00:00
abstract：:Insulin-deficient states are associated with an impaired function of the hypothalamic-pituitary-gonadal axis, but the mechanisms underlying hypothalamic alterations in experimental diabetes are still unknown. We investigated the effect of glucose concentrations, in the presence and absence of insulin, on LHRH release ...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000126257
更新日期：1992-09-01 00:00:00
abstract：:Gonadal steroids exert an important regulatory influence upon the biosynthetic and secretory activity of the somatostatin and growth hormone-releasing hormone (GHRH) neurons controlling the release of growth hormone. It is hypothesized that some of these effects occur in an indirect transsynaptic manner through the st...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000064428
更新日期：2002-08-01 00:00:00
abstract：:Studies were undertaken to characterize the secretion of corticotropin-releasing factor (CRF) and arginine vasopressin (AVP) into the hypophysial-portal circulation of the conscious sheep. In addition, we examined the temporal relationship between the secretion of these two hypothalamic peptides and the secretion of t...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000125141
更新日期：1989-04-01 00:00:00
abstract：:The response of folliculo-stellate (FS) cells of the anterior pituitary to estrogen has been studied in two strains of rat which differ in estrogen responsiveness. Fischer 344 (F344) rats are highly estrogen-responsive in comparison to Sprague-Dawley (S-D) rats. Ovariectomized adults were implanted with silastic capsu...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000125064
更新日期：1988-11-01 00:00:00
abstract：:To characterize the short-term ACTH secretion pattern and to investigate factors regulating it, pituitary venous (PV) blood was collected using our nonsurgical method from 8 unperturbed horses every 20 or 30 s for approximately 1 h. In all but 1 horse, sampling occurred during the broad circadian maximum in plasma cor...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000126755
更新日期：1994-09-01 00:00:00
abstract：:The arcuate nucleus-median eminence complex (AM) undergoes major structural and functional changes during normal puberty or if exposed to a pulse of estradiol in the prepuberal period. Those changes are expressed by increased synaptogenesis and by a drastic alteration in the feedback control of anterior pituitary glan...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000124775
更新日期：1987-06-01 00:00:00
abstract：UNLABELLED:Opioid peptides are present in both the posterior pituitary (PP) and stalk-median eminence (SME). Their effects on the dopaminergic neurons in the SME are well documented, but little is known concerning their role in the regulation of dopamine (DA) release from the PP. The objectives of this study were (1) t...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000125612
更新日期：1990-10-01 00:00:00
abstract：:Methanol extracts of rat plasma resulted in release of prolactin (PRL) from rat hemipituitaries in vitro with a linear log-dose relationship. This prolactin-releasing factor (PRF)-like activity was not altered in plasma from rats treated with bromocryptine or chlorpromazine despite significant suppression and stimulat...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000122925
更新日期：1979-01-01 00:00:00
abstract：:To determine the possible physiological role of endogenous growth hormone-releasing factor (GRF) in the neuronal content and release of cerebral somatostatin (SS), we studied the effect of endogenous GRF blockade on the immunoreactive SS (IR-SS) content of cells and media in fetal rat cerebral cortical and hypothalami...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000126118
更新日期：1992-02-01 00:00:00
abstract：:Our previous studies on annexin 5, a member of the annexin family of proteins, have shown its expression in the anterior pituitary gland, its preferential distribution in gonadotropes, and its increase after ovariectomy. In the present study, we examined (1) whether annexin 5 is synthesized in gonadotropes, (2) whethe...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000048216
更新日期：2002-01-01 00:00:00
abstract：:Rats bearing transplanted prolactin-secreting tumors are known to have very high prolactin levels in the serum, reduced hypothalamic opiate concentrations, increasing hypothalamic dopamine synthesis and increased hypophyseal portal blood dopamine levels. Further, it has been shown that incubation in 3H-leucine of ante...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000123211
更新日期：1981-08-01 00:00:00
abstract：BACKGROUND:Manipulating thyroid hormones has been shown to influence learning and memory. Although a large body of literature is available on the effects of thyroid hormone deficiency on learning and memory functions during developmental or adult-onset hypothyroidism, electrophysiological findings are limited. This lim...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000328513
更新日期：2011-01-01 00:00:00
abstract：:Neuroanatomical attempts have been made to determine the synapses between luteinizing hormone-releasing hormone (LHRH)-containing neurons and substance P (SP)-containing neurons in the hypothalamus of female rats. Wheat germ agglutinin was injected into the septo-preoptic area (SPA) and found to be incorporated into c...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000125724
更新日期：1991-03-01 00:00:00
abstract：:The effects of corticotropin-releasing factor (CRF) on GnRH release from the adult male rat mediobasal hypothalamus (MBH) and median eminence (ME) were studied in an in vitro incubation system. CRF induced a dose-related inhibition of GnRH release from both the MBH and the isolated ME, and this inhibition was blocked ...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000124578
更新日期：1986-01-01 00:00:00
abstract：:There is a great deal of conflicting data regarding the issue of whether androgens influence opiate receptors in the whole male rat brain. Although Hahn and Fishman initially reported that long-term castration produced a large increase in the density of opiate-binding sites, relative to controls, no other independent ...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000124816
更新日期：1987-08-01 00:00:00
abstract：:Cell-intact patch-clamp recording was used to determine the electrophysiological responses of sheep anterior pituitary gonadotropes to stimulation with gonadotropin-releasing hormone (GnRH). Cells were identified prior to recording by reverse haemolytic plaque assay (RHPA), or using morphological criteria in preparati...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000126605
更新日期：1993-12-01 00:00:00
abstract：:The effect of intraventricular and intravenous (i.v.) hypertonic saline on plasma and perfusate arginine vasopressin (AVP) and oxytocin (OT) levels was determined. A push-pull technique was used to sample third ventricular cerebrospinal fluid (CSF) in the conscious unrestrained rat. Intraventricular perfusion of hyper...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000124008
更新日期：1984-11-01 00:00:00
abstract：:His-dTrp-Ala-Trp-dPhe,Lys-NH2(GHRP-6) is a synthetic compound that releases GH in a dose-response and specific manner in several species and that may well be related to an endogenous compound of similar structure. The aim of this study was to investigate the in vivo GH responses to GHRP-6 in pentobarbital anesthetized...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000126366
更新日期：1993-01-01 00:00:00
abstract：:The present study was undertaken in order to better characterize the functional state of anterior pituitary gland in young and old rats by using prolactin secretion and incorporation of radioactive phosphate into phosphatidylinositol (PI) as markers. The in vitro incorporation of radiolabeled phosphate into anterior p...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000124714
更新日期：1987-02-01 00:00:00
abstract：:The effects of acute and chronic administration of a combination of lynestrenol-mestrenol, a widely employed contraceptive medication, on the dopaminergic system of the rat forebrain and striatum were investigated to better understand the biochemical basis of the neurological side-effects of steroid contraceptive drug...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000122643
更新日期：1976-01-01 00:00:00
