Abstract:
:The aims of this study were to determine whether endothelin-1 (ET-1), a positive inotropic agent, altered the production of cyclic AMP (cAMP) in adult feline cardiac myocytes and to characterize the effect with respect to G-protein-coupling and calcium regulation of adenylyl cyclase. ET-1 inhibited basal and/or stimulated cAMP accumulation in the intact cardiac myocyte and in membrane preparations in a dose-dependent manner. In intact cells, maximal inhibition of forskolin-stimulated cAMP accumulation was 90-95% with an EC50 of 5 x 10(-10) M. Inhibition of isoproterenol-stimulated cAMP was biphasic with maximal inhibition of 70% observed by 10(-11)M; at higher doses inhibition was not consistently observed. The inhibitory response to ET-1 occurred in the absence or presence of isobutylmethylxanthine suggesting that activation of cAMP phosphodiesterases was not the means for reducing cAMP levels. Prior exposure of cardiac myocytes to 100ng/ml pertussis toxin blocked the inhibitory action of ET-1, indicating that this response is mediated through the involvement of a pertussis toxin-sensitive G-protein such as Gi. Studies carried out in the absence of extracellular calcium and under conditions of cell-loading with the intracellular calcium chelator, 1,2-bis-(2-aminophenoxy)-ethane-N,N,N'N'-tetraacetic acid-acetoxymethyl ester (BAPTA/AM), suggest that the mechanism by which ET-1 inhibits cAMP accumulation is not calcium-dependent. Thus, inhibition of cAMP accumulation by ET-1 appears to be mediated through a pertussis toxin sensitive protein rather than by activation of phosphodiesterases or calcium inhibition of cardiac forms of adenylyl cyclase. Though unlikely to play a role in the positive inotropic effect of ET-1, transduction of ET-1 responses through Gi suggests another means for regulation of growth in these adult cardiac myocytes.
journal_name
Life Scijournal_title
Life sciencesauthors
Jones LGdoi
10.1016/0024-3205(95)02264-3subject
Has Abstractpub_date
1996-01-01 00:00:00pages
115-23issue
2eissn
0024-3205issn
1879-0631pii
0024320595022643journal_volume
58pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract::[3H]-Spiroperidol specifically binds at sites in human prefrontal cortex. The binding of this ligand is apparently anomalous at 37 degrees C, with a substantial loss of specific binding occurring between 5 and 40 min incubation. However, at 21 degrees C, this loss of binding is not observed even at 60 min. At 21 degre...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(84)90282-0
更新日期:1984-06-18 00:00:00
abstract::Plasma and urinary levels of MHPG were determined in six normal volunteers. Samples were obtained at 3-hour intervals for plasma and at 12-hour intervals for urine. Acrophase, amplitude and period were determined for plasma MHPG levels. A sinusoidal pattern was obtained for diurnal plasma MHPG with a peak at 15:00 hrs...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(85)90302-9
更新日期:1985-11-04 00:00:00
abstract::Promoter methylation is an important mechanism of regulating E-cadherin expression. Methylation-specific PCR (MSP) assay was done to evaluate the promoter methylation status of E-cadherin gene in primary tumor samples from 23 cases of Chinese women with invasive ductal breast cancers. Western blotting assay was employ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(02)01843-x
更新日期:2002-08-09 00:00:00
abstract:AIMS:Brain injuries based on their causes are divided into two categories, TBI and NTBI. TBI is caused by damages such as head injury, but non-physical injury causes NTBI. Prolactin is one of the blood factors that increase during brain injury. It has been assumed to play a regenerative role in post-injury recovery. M...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2020.118547
更新日期:2020-12-15 00:00:00
abstract::An experimental model which resembles human drug addiction was developed to study the effect of chronic drug (cocaine or morphine) administration on the immune system. As malnutrition has been associated with drug use, a low protein diet has been evaluated for its contribution to the impairment of the immune system du...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(91)90138-2
更新日期:1991-01-01 00:00:00
abstract::The objective of this study was to investigate whether age induces changes on vasodilator response induced by cromakalim, an ATP-sensitive K+ (K(ATP)) channel opener, as well as the underlying mechanism involved in this possible alteration. For this purpose, aortic segments from young (3-5 months) and old (3 years) ra...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(99)80003-4
更新日期:1998-01-01 00:00:00
abstract:AIMS:Constant development of chemotherapeutic strategies has considerably improved the efficiency of tumor treatment. However, adverse effects of chemotherapeutics enforce premature treatment cessation, which leads to the tumor recurrence and accelerated death of oncologic patients. Recently, sodium ascorbate (ASC) has...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2019.116657
更新日期:2019-09-01 00:00:00
abstract::Aminoalkylindoles (AAIs) are structurally dissimilar from the classical cannabinoids (CCs), however, both AAIs and CCs appear to bind at the same site on the cannabinoid receptor. To obtain better insights on the structural correlation between AAIs and CCs, we have studied the conformational properties of the potent c...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(95)00177-8
更新日期:1995-01-01 00:00:00
abstract::Repeated intermittent administration of amphetamine in mice caused reverse tolerance to 5-hydroxy-L-tryptophan (5-HTP)-induced head twitch, as well as to amphetamine-induced stereotypy. The repeated administration of 5-HTP alone also resulted in reverse tolerance in the head-twitch test. Daily pretreatment with halope...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(90)90141-d
更新日期:1990-01-01 00:00:00
abstract::Ipriflavone, 7-isopropoxy-3-phenyl-4H-1-benzopyran-4-one, was administered orally for 12 weeks to male rats with prednisolone-induced osteoporosis. Microdensitometric analysis of a roentgenograph of the femurs revealed that ipriflavone increased the density of the distal metaphysis dose-dependently and tended to incre...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(86)90265-1
更新日期:1986-03-10 00:00:00
abstract:BACKGROUND:Cisplatin resistance has been found to contribute to the failure of ovarian carcinoma treatment. Oridonin is a natural en-kaurane tetracyclic diterpenoid compound discovered in Rabdosia rubescene (Henmsl.) Hara. Herein, we tested whether oridonin could exert chemo-sensitization activity on cisplatin-resistan...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2019.116709
更新日期:2019-09-15 00:00:00
abstract::Nucleotides, e.g. ATP and ADP, are important signaling molecules, which elicit several biological responses. The degradation of nucleotides is catalyzed by a family of enzymes called NTPDases (nucleoside triphosphate diphosphohydrolases). The present study reports the enzymatic properties of a NTPDase (CD39, apyrase, ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(03)00596-4
更新日期:2003-09-05 00:00:00
abstract::Ethanol-induced emesis were investigated using Suncus murinus and the emetogenic mechanisms of ethanol were compared with those of cisplatin. Intraperitoneal injection of ethanol caused dose-dependent emesis with ED50 value of 22.3% (v/v) when injection volume was adjusted to 4 ml/kg. Intraperitoneal and subcutaneous ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(96)00625-x
更新日期:1997-01-01 00:00:00
abstract::The effects of erythropoietin (EPO) on cytosolic free calcium concentration ([Ca2+]i) in platelets of 20 essential hypertensive patients (HT) and of 25 normotensive subjects (NT) were investigated using the fura2 technique. In resting platelets [Ca2+]i were not significantly higher in HT compared to NT (74.3 +/- 7.8 n...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(92)90010-m
更新日期:1992-01-01 00:00:00
abstract:AIMS:Dyslipidemia-associated diabetic retinopathy is featured by macular edema and retinal angiogenesis. This study investigated the in vitro lipotoxicity of free fatty acids and their modulatory roles in regulation of autophagy and angiogenic factor production in cultured human retinal pigment epithelium (RPE) ARPE-19...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2020.118391
更新日期:2020-10-15 00:00:00
abstract:AIMS:High level of saturated fatty acids leads to mitochondrial dysfunction and inflammation in the development of insulin resistance in skeletal muscle. We recently found that puerarin improved impaired insulin signaling in skeletal muscle in diabetic animals and in myotubes in vitro. However, whether puerarin can act...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2018.05.041
更新日期:2018-08-01 00:00:00
abstract::In frog brain membranes, equilibrium saturation binding of the opiate agonist: 3H-etorphine and of the opiate antagonist: 3H-diprenorphine is biphasic. In the presence of sodium ions (120 mM), "kinetic conversions" (unchanged Bmax) are observed: high affinity binding of the 3H-agonist is decreased while high affinity ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(83)90477-0
更新日期:1983-01-01 00:00:00
abstract::The effects of a newly synthesized compound, 7-(3-[4-(2,3-dimethylphenyl)piperazinyl]propoxy)-2(1H)-quinolinone (OPC-4392), on tyrosine hydroxylation in situ and in vitro were studied using rat striatal slices and tyrosine hydroxylase (TH) purified from bovine adrenal medulla, respectively. OPC-4392 dose-dependently i...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(88)90644-3
更新日期:1988-01-01 00:00:00
abstract:AIMS:Two natural alkaloids, methyl (M) and isopropyl (I) N-methylanthranilates, with recently demonstrated significant pharmacological activities, were assayed for their possible overall effect on intact gastric mucosa and their protective properties towards the onset of gastric lesions induced by diclofenac (a non-ste...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2013.10.002
更新日期:2013-11-19 00:00:00
abstract::Evidence for a drug-induced activation of central sigma systems is presented. The model is the locomotor activation initiated by a subcutaneous (SC) challenge of 1.6 mg/kg of (+)-butaclamol, (+)-BUT, given 30 min before 10 mg/kg SC of (-)-N-allylnormetazocine, (-)-NAN, in Sprague-Dawley male rats which have been pretr...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(89)90448-7
更新日期:1989-01-01 00:00:00
abstract::Tissue copper levels of brindled (Mobr) mice and normal litter-mates after single and repeated dosing with CuCl2 and/or D-penicillamine are examined, together with a study of the cytosol distribution of copper after CuCl2 treatment. The results confirm that the mutant mouse kidney is capable of extensive copper accumu...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(82)90326-5
更新日期:1982-08-02 00:00:00
abstract::Recently, 4.4'-bis(1-p-carboxyphenyl-3-methyl-5-dydroxyl)-pyrazol (DRD156) has been developed as a new sensitive reagent that reacts specifically with singlet oxygen. The specificity of DRD156 for singlet oxygen in a biomimetic solution (micellar solution) and the effects of its coexistence with other reagent were exa...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(02)02234-8
更新日期:2002-12-06 00:00:00
abstract::SARS-CoV-2 infection or COVID-19 has become a worldwide pandemic; however, effective treatment for COVID-19 remains to be established. Along with acute respiratory distress syndrome (ARDS), new and old cardiovascular injuries are important causes of significant morbidity and mortality in COVID-19. Exploring new approa...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2021.119130
更新日期:2021-01-26 00:00:00
abstract:AIMS:The long non-coding RNA (lncRNA) was reported to be involved in the progress of various cancers, however, its effect in colorectal cancer (CRC) remains unknown. The goal of the present study is to investigate the function role of lncRNA PCAT-1 in colorectal cancer. MAIN METHODS:The expression of lncRNA PCAT-1 in ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2017.08.024
更新日期:2017-11-01 00:00:00
abstract::The effects of t-butylhydroperoxide (TBH) and cigarette smoke on lung mechanics (CDYN and RL) and glutathione status (GSH) were studied using an isolated perfused and ventilated rat lung preparation. TBH (200, 400, 1000 microM) infused via the pulmonary circulation caused a dose-related bronchoconstriction. The lung G...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(88)90054-9
更新日期:1988-01-01 00:00:00
abstract::Several different vasodilator substances can be released by vascular endothelium in response to mechanical stimuli and vasoactive agents. The purpose of this study was to determine whether there is a male-female difference in the relative contributions of nitric oxide (NO) and endothelium-derived hyperpolarizing facto...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(02)01851-9
更新日期:2002-08-23 00:00:00
abstract::Superoxide dismutase (SOD) levels, thought to be the first cellular defence against free radicals, were studied in the nonobese diabetesprone (NOD-p) mouse, an animal model of type 1 diabetes in which about 100% of females and 20% of males become diabetic. Nonobese diabetes nonprone (NON-p) mice were used as controls....
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(95)00211-n
更新日期:1995-01-01 00:00:00
abstract::The aggregation of melanin-granules within fish pigment cells (melanophores) can be elicited either by electrical stimulation of intrinsic nerves or by the addition of adrenergic agonists. The pigment aggregation seems to be mediated by alpha-2-adrenoceptors. In this investigation we have used various agonists and ant...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(85)90595-8
更新日期:1985-09-16 00:00:00
abstract::The aim of the present study was to assess the effects of peanut (Arachis hypogaea L.) shell extracts (PSE) on lipases and to evaluate its potential development for the treatment of obesity. The peanut shells were extracted in 95% ethanol, and the extracts were screened for inhibitory effects on pancreatic lipase (PL)...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2005.11.012
更新日期:2006-05-08 00:00:00
abstract::Botanical sources for medicines in America have been known since long before the arrival of Columbus. Nevertheless, both scientists and the general public are often unaware that some of these botanical drugs are also potent intoxicants. We provide a quick overview of hallucinogenic and dissociative drugs harvested fro...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2005.09.005
更新日期:2005-12-22 00:00:00