Abstract:
:1. Patch-clamp methods were used to study recombinant glutamate receptor (GluR) ion channels expressed in human embryonic kidney cells (HEK 293) after transfection of the cells with cDNAs encoding kainate (KA)-type GluR subunits. Cells were transiently or stably transfected with the fully edited (R) version of GluR6 or they were contransfected with GluR6(R) and KA2. 2. Concentration-response data were obtained for KA and glutamate activation of homomeric GluR6(R) channels and fitted with Hill-type equations to give values for the agonist concentration at half-maximal activation (EC50), the Hill coefficient (nH), and the maximum current (Imax). Analysis of results obtained in seven cells with KA gave mean values of 0.47 microM and 1.47 for the EC50 and nH, respectively. The corresponding values for glutamate were 32 microM and 1.21 (n = 5). The Imax values obtained for KA and glutamate in the same cells were similar, suggesting that both KA and glutamate are full agonists at homomeric GluR6(R) channels. 3. Spectral density analysis of current noise evoked by KA and glutamate in GluR6(R)-expressing cells, or in outside-out patches from these cells, was used to obtain estimates of the apparent unitary conductance (gamma noise) of homomeric GluR6(R) channels. Results obtained with 10 microM KA in 15 cells gave a mean gamma noise value of 231 fS. The corresponding value from analysis of noise in outside-out patches was 259 fS (n = 4). Spectral density analysis of glutamate-evoked noise in six cells gave a mean gamma noise value of 264 fS. 4. Noise analysis of whole cell currents recorded in GluR6(R)/ KA2-expressing cell gave a mean gamma noise value of 572 fS (n = 9). Unlike homomeric GluR6(R) channels, GluR6(R)/KA2 heteromers were activated by alpha-amino-3-hydroxy-5-methyl-isoxazole-4-propionic acid (AMPA) (0.25-5 mM). The mean EC50 for KA activation of GluR6(R)/KA2 channels was 1.62 microM (n = 6). 5. The results indicate that both homomeric GluR6(R) and heteromieric GluR6(R)/KA2 channels have a unitary conductance in the femtosiemen range. The coassembly of KA2 with GluR6(R) results in channels that have a different affinity for AMPA and KA and a two- to threefold larger unitary conductance.
journal_name
J Neurophysioljournal_title
Journal of neurophysiologyauthors
Howe JRdoi
10.1152/jn.1996.76.1.510subject
Has Abstractpub_date
1996-07-01 00:00:00pages
510-9issue
1eissn
0022-3077issn
1522-1598journal_volume
76pub_type
杂志文章abstract::Previous studies have reported that the translational vestibuloocular reflex (TVOR) follows a three-dimensional (3D) kinematic behavior that is more similar to visually guided eye movements, like pursuit, rather than the rotational VOR (RVOR). Accordingly, TVOR rotation axes tilted with eye position toward an eye-fixe...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.01137.2002
更新日期:2003-05-01 00:00:00
abstract::Depolarization-induced suppression of inhibition (DSI) is a form of retrograde signaling at GABAergic synapses that is initiated by the calcium- and depolarization-dependent release of endocannabinoids from postsynaptic neurons. In the neocortex, pyramidal neurons (PNs) appear to use DSI as a mechanism for regulating ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00351.2004
更新日期:2004-10-01 00:00:00
abstract::Synapsin is a synaptic vesicle-associated protein implicated in the regulation of vesicle trafficking and transmitter release, but its role in heterosynaptic plasticity remains elusive. Moreover, contradictory results have obscured the contribution of synapsin to homosynaptic plasticity. We previously reported that th...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00604.2007
更新日期:2007-12-01 00:00:00
abstract::The responses of spinothalamic tract neurons were studied by extra- and intracellular recordings from the lumbosacral spinal cord in anesthetized rhesus monkeys (Macaca mulatta). The neurons were identified by antidromic activation from the contralateral diencephalon. They were then classified by the mildest form of m...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1975.38.1.132
更新日期:1975-01-01 00:00:00
abstract::The activity of jaw muscle receptors was studied by recording neurons in the mesencephalic nucleus of the trigeminal nerve in monkeys trained to control the position and movement of their mandible. Jaw position was measured by a weighted lever resting on the mandibular incisors. The force required to maintain the posi...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1983.50.1.61
更新日期:1983-07-01 00:00:00
abstract::1. The antidromic compound action potential (ACAP) of the auditory nerve was evoked by shocks to the auditory nerve root and recorded at the round window of the cochlea in anesthetized guinea pigs. The goal of this study was to determine the characteristics of the ACAP and compare these characteristics with those of t...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1994.71.5.1826
更新日期:1994-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1152/jn.00507.2010
更新日期:2011-01-01 00:00:00
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journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1990.64.4.1291
更新日期:1990-10-01 00:00:00
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journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1998.79.5.2460
更新日期:1998-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:2000-01-01 00:00:00
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journal_title:Journal of neurophysiology
pub_type: 杂志文章
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更新日期:2019-08-01 00:00:00
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journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1983.50.5.1059
更新日期:1983-11-01 00:00:00
abstract::Odors activate lobster olfactory receptor neurons (ORNs) through phosphoinositide signaling that appears to target a Na(+)-gated nonselective cation channel. The Na(+)-gated channel is a potential member of the growing family of transient receptor potential (TRP) channels. Here, we test the effect of potential antagon...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00990.2004
更新日期:2005-03-01 00:00:00
abstract::1. The role of the microgeometry of planar surfaces in the detection of sliding of the surfaces on human and monkey fingerpads was investigated. By the use of a servo-controlled tactile stimulator to press and stroke glass plates on passive fingerpads of human subjects, the ability of humans to discriminate the direct...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1990.63.6.1323
更新日期:1990-06-01 00:00:00
abstract::In adult songbirds, auditory neurons in the primary auditory forebrain region of field L and a secondary auditory forebrain region of caudal mesopallium (CM) are highly responsive to natural sounds, such as conspecific song. Because these nuclei are involved in sensory representations of songs, we investigated how the...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.01066.2006
更新日期:2007-05-01 00:00:00
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journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00395.2005
更新日期:2006-02-01 00:00:00
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journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1997.77.6.2975
更新日期:1997-06-01 00:00:00
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journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.2000.83.2.639
更新日期:2000-02-01 00:00:00
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journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1994.72.3.1395
更新日期:1994-09-01 00:00:00
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journal_title:Journal of neurophysiology
pub_type: 杂志文章
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更新日期:2010-03-01 00:00:00
abstract::To elucidate how information is processed in the vestibuloocular reflex (VOR) pathways subserving vertical eye movements, extracellular single-unit recordings were obtained from the vestibular nuclei of alert monkeys trained to track a visual target with their eyes while undergoing sinusoidal pitch oscillations (0.2-1...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1984.52.4.724
更新日期:1984-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:2006-07-01 00:00:00
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journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1998.79.2.769
更新日期:1998-02-01 00:00:00
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journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.2000.83.4.2002
更新日期:2000-04-01 00:00:00
abstract::The general objectives of our research, presented in this series of papers, were to develop a computational model of the brain stem respiratory neural network and to explore possible neural mechanisms that provide the genesis of respiratory oscillations and the specific firing patterns of respiratory neurons. The pres...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1997.77.4.1994
更新日期:1997-04-01 00:00:00
abstract::In the absence of any obvious input, sensory neurons and interneurons can display resting or spontaneous activity. This is often regarded as noise and removed through trial averaging, although it may reflect history-dependent modulation of tuning or fidelity and, thus, be of functional relevance to downstream interneu...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00864.2018
更新日期:2019-12-01 00:00:00
abstract::The axons that project into the striatum are known to segregate according to macroscopic cortical systems; however, the within-region organization of these fibers has yet to be described in humans. We used in vivo fiber tractography, in neurologically healthy adults, to map white matter bundles that originate in diffe...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00995.2011
更新日期:2012-06-01 00:00:00
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journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00905.2015
更新日期:2016-03-01 00:00:00
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journal_title:Journal of neurophysiology
pub_type: 杂志文章
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更新日期:1998-10-01 00:00:00
abstract::One of the critical factors in determining network behavior of neurons is the influence of local circuit connections among interneurons. The short-term synaptic plasticity and the subtype of presynaptic calcium channels used at local circuit connections of inhibitory interneurons in CA1 were investigated using dual wh...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
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更新日期:2011-03-01 00:00:00