Autophagy protects type II alveolar epithelial cells from Mycobacterium tuberculosis infection.

abstract：:We identified a DNA polymerase species in Drosophila melanogaster embryos, and purified it. This polymerase shared some common properties with DNA polymerase epsilon from mammals and yeast as follows; it has a preference for poly(dA)/oligo(dT) as a template/primer, it is highly processive in DNA synthesis, it co-fract...

journal_title：Biochemical and biophysical research communications

authors： Aoyagi N,Oshige M,Hirose F,Kuroda K,Matsukage A,Sakaguchi K

更新日期：1997-01-13 00:00:00

abstract：:Removal of parts of a known calmodulin binding site at the C-terminus of HIV-1 envelope glycoprotein, gp160, can result in diminished infectivity. We investigated whether expression of full length gp160 would result in changes in intracellular calmodulin compared to expression of gp160 truncated to remove both known c...

journal_title：Biochemical and biophysical research communications

authors： Radding W,Pan ZQ,Hunter E,Johnston P,Williams JP,McDonald JM

更新日期：1996-01-05 00:00:00

abstract：:An endonuclease that specifically removes 8-hydroxyguanine (oh8Gua) from DNA has been isolated from Escherichia coli. As the amount of oh8Gua produced in DNA of X-ray-irradiated mice is known to decrease with time after irradiation, an attempt was made to find a similar activity in human polymorphonuclear neutrophils ...

journal_title：Biochemical and biophysical research communications

authors： Chung MH,Kim HS,Ohtsuka E,Kasai H,Yamamoto F,Nishimura S

更新日期：1991-08-15 00:00:00

abstract：:The ability of exogenous nitric oxide (NO) to induce apoptosis in macrophages was analyzed using NOC, a NO-releasing compound, as a source of NO. Exogenous NO was shown to induce apoptosis in a dose dependent manner. Quantitative analysis revealed that the amount of NO required to induce apoptosis in more than half of...

journal_title：Biochemical and biophysical research communications

authors： Shimaoka M,Iida T,Ohara A,Taenaka N,Mashimo T,Honda T,Yoshiya I

更新日期：1995-04-17 00:00:00

abstract：:Epidemiological and experimental data suggest a role for polyunsaturated fatty acids in the etiology of breast cancer. In this report we have studied arachidonic acid and linoleic acid metabolism in the human breast carcinoma cell line BT-20 which overexpresses both EGF receptor and the homologous erbB-2 oncogene prod...

journal_title：Biochemical and biophysical research communications

authors： Reddy N,Everhart A,Eling T,Glasgow W

更新日期：1997-02-03 00:00:00

abstract：:Adrenomedullin (ADM) is a vasodilator peptide, first isolated from human pheochromocytoma. To explore the pathophysiological role of ADM in ischemic conditions, we investigated the effects of hypoxia on ADM production and ADM mRNA expression in a cultured human colorectal carcinoma cell line, DLD-1. Northern blot anal...

journal_title：Biochemical and biophysical research communications

authors： Nakayama M,Takahashi K,Murakami O,Shirato K,Shibahara S

更新日期：1998-02-13 00:00:00

abstract：:Vasoactive intestinal peptide (VIP) is a small neuropeptide, which exerts pleiotropic functions. Based on its immunomodulatory, secretory, and possibly trophic effects, VIP is a valuable candidate molecule for the management of autoimmune disease. The purpose of this study was to develop a recombinant viral vector cap...

journal_title：Biochemical and biophysical research communications

authors： Lodde BM,Delporte C,Goldsmith CM,Tak PP,Baum BJ

更新日期：2004-06-18 00:00:00

abstract：:An intein is a polypeptide that interrupts the functional domains of a protein, called the exteins. The intein can facilitate its own excision from the exteins, concomitant with the ligation of the exteins, in a process called protein splicing. The alpha subunit of the ribonucleotide reductase of the extreme thermophi...

journal_title：Biochemical and biophysical research communications

authors： Kerrigan AM,Powers TL,Dorval DM,Reitter JN,Mills KV

更新日期：2009-09-11 00:00:00

abstract：:The activity of the enzyme tyrosine aminotransferase and the synthesis of its specific mRNA were evaluated at different hours of the day in the liver of 3-, 12- and 24-month old BN rats. The enzyme activity has a circadian rhythm with a peak at midnight in 3- and 12-month old, which shifts to 03.00 hrs in 24-month old...

journal_title：Biochemical and biophysical research communications

authors： Servillo G,Della Fazia MA,Viola-Magni M

更新日期：1991-02-28 00:00:00

abstract：:The calcium homeostasis of the endoplasmic reticulum (ER) is connected to a multitude of cell functions involved in intracellular signal transduction, control of proliferation, programmed cell death, or the synthesis of mature proteins. Calcium is accumulated in the ER by various biochemically distinct sarco/endoplasm...

journal_title：Biochemical and biophysical research communications

authors： Papp B,Brouland JP,Gélébart P,Kovàcs T,Chomienne C

更新日期：2004-10-01 00:00:00

abstract：:An inhibitor against the egg beta-D-N-acetylglucosaminidase (GlcNAcase) was purified from the vitelline coat of eggs of the ascidian, Halocynthia roretzi. The purified inhibitor gave a single band with an apparent molecular mass of 70 kDa on SDS-PAGE under non-reducing conditions. The GlcNAcase inhibitor potently inhi...

journal_title：Biochemical and biophysical research communications

authors： Matsuura K,Sawada H,Yokosawa H

更新日期：1995-08-04 00:00:00

abstract：:A Bacillus thuringiensis isolate, 89-T-34-22, belonging to the serovar shandongiensis (H22) produced noninsecticidal and nonhemolytic proteins crystallizing into irregular-shaped parasporal inclusions. The proteins showed in vitro cytotoxicity to human cells, including cancer cells, only when activated by protease tre...

journal_title：Biochemical and biophysical research communications

authors： Lee DW,Akao T,Yamashita S,Katayama H,Maeda M,Saitoh H,Mizuki E,Ohba M

更新日期：2000-05-27 00:00:00

abstract：:Resistin is an adipokine related to obesity and insulin resistance. Expression of the resistin gene is repressed by the treatment of peroxisome proliferator-activated receptor gamma (PPARgamma) agonists, thiazolidinediones (TZDs). In this study, we investigated the mechanism by which TZDs inhibit the resistin gene exp...

journal_title：Biochemical and biophysical research communications

authors： Chung SS,Choi HH,Cho YM,Lee HK,Park KS

更新日期：2006-09-15 00:00:00

abstract：:Blockage of either nuclear factor-kappaB (NF-kappaB) or Akt sensitizes cancer cells to TNF-induced apoptosis. In this study, we investigated the undetermined effect of concurrent blockage of these two survival pathways on TNF-induced cytotoxicity in lung cancer cells. The results show that Akt contributes to TNF-induc...

journal_title：Biochemical and biophysical research communications

authors： Wang X,Chen W,Lin Y

更新日期：2007-04-13 00:00:00

abstract：:Ghrelin, a novel growth-hormone-releasing acylated peptide, was recently isolated from rat stomach by the search of an endogenous ligand to an "orphan" G-protein-coupled-receptor. Ghrelin neuron is present in the arcuate nucleus of rat hypothalamus, but its central effect on growth hormone (GH) release has yet to be c...

journal_title：Biochemical and biophysical research communications

authors： Date Y,Murakami N,Kojima M,Kuroiwa T,Matsukura S,Kangawa K,Nakazato M

更新日期：2000-08-28 00:00:00

abstract：:The effect of tyrosine-phosphorylation on the conformation of three tyrosine-based immunoreceptor activation motifs, zeta(69-86), zeta(106-126), and zeta(138-155), located in the T cell receptor/CD3 zeta-chain was investigated. Circular dichroism and Fourier-transform infrared spectroscopy of the nonphosphorylated and...

journal_title：Biochemical and biophysical research communications

authors： Laczkó I,Hollósi M,Vass E,Hegedüs Z,Monostori E,Tóth GK

更新日期：1998-01-26 00:00:00

abstract：:Using isolated cartilage cells from the epiphyseal growth plate of rachitic chicks and utilizing the fluorescence indicator quin2 for measurements of cytosolic free calcium, it has been possible to demonstrate that 1,25-dihydroxyvitamin D3 regulates the concentrations of cytosolic free calcium. The reduction in cytoso...

journal_title：Biochemical and biophysical research communications

authors： Bar S,Lidor C,Harell A,Edelstein S

更新日期：1986-12-30 00:00:00

abstract：:A simple and high yield purification procedure for the isolation of F0F1-ATPase from spinach leaf mitochondria has been developed. This is the first report concerning purification and composition of the plant mitochondrial F0F1-ATPase. The enzyme is selectively extracted from inner membrane vesicles with the zwitterio...

journal_title：Biochemical and biophysical research communications

authors： Hamasur B,Glaser E

更新日期：1990-08-16 00:00:00

abstract：:The effects of endothelins (ETs) on the production of stromelysins, a sub-family of matrix metalloproteinases, were examined in cultured astrocytes. The treatment of cultured rat astrocytes with ET-1 increased stromelysin-1 mRNA levels, while stromelysin-2 and -3 mRNAs were not affected. Immunocytochemical observation...

journal_title：Biochemical and biophysical research communications

authors： Koyama Y,Tanaka K

更新日期：2008-07-11 00:00:00

abstract：:Behcet's disease (BD) is a chronic relapsing inflammatory autoimmune disease characterized by recurrent oral and genital ulcers, skin legions and uveitis and its pathogenesis is not fully elucidated. Previously we identified that two novel susceptible SNPs are associated with BD. One is located in putative RNF39 promo...

journal_title：Biochemical and biophysical research communications

authors： Kurata R,Tajima A,Yonezawa T,Inoko H

更新日期：2013-06-21 00:00:00

abstract：:A comparison was made of cytosynalin- and MAPs-induced microtubule formation. Cytosynalin (a 35 kDa cytoskeleton-interacting and calmodulin-binding protein) facilitated more rapid and extensive microtubule formation than MAPs. Electron microscopic examination revealed that the cytosynalin-induced microtubules were 24 ...

journal_title：Biochemical and biophysical research communications

authors： Itoh K,Sobue K

更新日期：1987-07-31 00:00:00

abstract：:The crystal structure of the mu and kappa opioid receptors has revealed dimeric structural arrangements. Mu-delta receptors heteromers also exist and we have identified discrete cytoplasmic regions in each receptor required for oligomer formation. In the carboxyl tail of the delta receptor we identified three glycine ...

journal_title：Biochemical and biophysical research communications

authors： O'Dowd BF,Ji X,O'Dowd PB,Nguyen T,George SR

更新日期：2012-06-15 00:00:00

abstract：:We previously reported that fucoidan, a dietary fiber purified from seaweed, inhibited IgE production by B cells in vitro. In this study, we examined the effect of fucoidan on IgE production in vivo. The OVA-induced increase of plasma IgE was significantly suppressed when fucoidan was intraperitoneally, but not orally...

journal_title：Biochemical and biophysical research communications

authors： Yanase Y,Hiragun T,Uchida K,Ishii K,Oomizu S,Suzuki H,Mihara S,Iwamoto K,Matsuo H,Onishi N,Kameyoshi Y,Hide M

更新日期：2009-09-25 00:00:00

abstract：:Entry of human immunodeficiency virus type 1 (HIV-1) virion into host cells involves three major steps, each being a potential target for the development of entry inhibitors: gp120 binding to CD4, gp120-CD4 complex interacting with a coreceptor, and gp41 refolding to form a six-helix bundle. Using a D-amino acid decap...

journal_title：Biochemical and biophysical research communications

authors： Boggiano C,Jiang S,Lu H,Zhao Q,Liu S,Binley J,Blondelle SE

更新日期：2006-09-08 00:00:00

abstract：:The repair of point mutations in hprt gene by single-stranded oligonucleotides represents a model to test targeted nucleotide exchange. We studied the concurrent nucleotide exchange of two or three nucleotides in the hprt deficient hamster cell line V79-151. The used oligonucleotides resulted in mismatches at two (151...

journal_title：Biochemical and biophysical research communications

authors： Kenner O,Lutomska A,Speit G,Vogel W,Kaufmann D

更新日期：2004-09-03 00:00:00

abstract：:A representative phenotype of patients with tricho-rhino-phalangeal syndrome (TRPS) is sparse hair. To understand the developmental defects of these patient's hair follicles, we analyzed the development of hair follicles histologically and biochemically using Trps1 deficient (KO) mice. First, we compared the numbers o...

journal_title：Biochemical and biophysical research communications

authors： Sun Y,Nakanishi M,Sato F,Oikawa K,Muragaki Y,Zhou G

更新日期：2015-01-16 00:00:00

abstract：:Placental choriocarcinoma, a reproductive system carcinoma in women, has about 0.81% occurrence frequency in China, which leads to over 90% lethality due to indistinct pathogenesis and the absence of efficient therapeutic treatment. In the present study, using immunostaining and reverse transcription PCR, we reported ...

journal_title：Biochemical and biophysical research communications

authors： Peng S,Miao C,Li J,Fan X,Cao Y,Duan E

更新日期：2006-11-24 00:00:00

abstract：:GOTO cells, a neuroblastoma cell line retaining the ability to differentiate into neuronal or Schwann cells, were found to be rich in membrane rafts containing ganglioside GM2 and hypersensitive to lipid raft-disrupting methyl-beta-cyclodextrin (MbetaCD); the GM2-rich rafts and sensitivity to MbetaCD were markedly dim...

journal_title：Biochemical and biophysical research communications

authors： Tomioka R,Minami N,Kushida A,Horibe S,Izumi I,Kato A,Fukushima K,Ideo H,Yamashita K,Hirose S,Saito Y

更新日期：2009-11-06 00:00:00

abstract：:Concomitant with the depletion of glutathione content, phorone (250 mg/kg, ip.) produced a marked increase in heme oxygenase activity, biphasic effect on delta-aminolevulinic acid synthetase activity, and slight decreases in cytochrome P-450 content and aminopyrine demethylase activity in the liver of rats. The increa...

journal_title：Biochemical and biophysical research communications

authors： Yoshida T,Oguro T,Numazawa S,Kuroiwa Y

更新日期：1987-05-29 00:00:00

abstract：:Apolipoprotein (apo-) B mRNA editing at nucleotide 6666 converts cytidine to uridine, transforming the codon for glutamine-2153 to a termination codon. To investigate this editing mechanism, [a-32P] and [5-3H] CTP were incorporated into synthetic apo-B RNA. After the substrate had been edited extensively in vitro by a...

journal_title：Biochemical and biophysical research communications

authors： Johnson DF,Poksay KS,Innerarity TL

更新日期：1993-09-30 00:00:00