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DNA polymerase epsilon from Drosophila melanogaster.

Abstract：

:We identified a DNA polymerase species in Drosophila melanogaster embryos, and purified it. This polymerase shared some common properties with DNA polymerase epsilon from mammals and yeast as follows; it has a preference for poly(dA)/oligo(dT) as a template/primer, it is highly processive in DNA synthesis, it co-fractionates with 3'-5' exonuclease activity, it is sensitive to aphidicolin and is resistance to ddTTP. The polymerase activity was inhibited in the immuno-precipitation assay with anti-pol-epsilon antibodies, which were produced against a polypeptide coded on the cDNA of a putative Drosophila pol-epsilon we isolated previously. Using these antibodies, Western blot analysis revealed that this polymerase is a 250kDa polypeptide, which is the same size as observed in mammals and yeast. These results indicate that Drosophila produces the epsilon-class of DNA polymerase, and like mammals or yeast, possesses the 5 typical classes of DNA polymerases (alpha to epsilon) in its embryos.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Aoyagi N,Oshige M,Hirose F,Kuroda K,Matsukage A,Sakaguchi K

doi

10.1006/bbrc.1996.5945

subject

Has Abstract

pub_date

1997-01-13 00:00:00

pages

297-301

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006291X96959458

journal_volume

230

pub_type

杂志文章

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