Purification and identification of endogenous anti-opioid substances from bovine brain.

abstract：:Cullin-RING ligases (CRLs) regulate diverse cellular functions such as cell cycle progression and cytokine signaling by ubiquitinating key regulatory proteins. The activity of CRLs is controlled by Nedd8 modification of the cullin subunits. Recent reports have suggested that CAND1, which specifically binds to unmodifi...

journal_title：Biochemical and biophysical research communications

authors： Min KW,Kwon MJ,Park HS,Park Y,Yoon SK,Yoon JB

更新日期：2005-09-02 00:00:00

abstract：:Exposure of pancreatic islet B-cells to D-glucose and many other insulinotropic agents results in an increase of cytoplasmic calcium concentration, which triggers the exocytosis of secretroy granules. Previous studies have demonstrated that calcium itself, at concentrations ranging from 2 to 18 mM, is able to induce a...

journal_title：Biochemical and biophysical research communications

authors： Rasschaert J,Malaisse WJ

更新日期：1999-11-02 00:00:00

abstract：:Adriamycin was used in situ, in isolated liver mitochondria of hyperthyroid rats to study the role of cardiolipin in the functioning of FAD-linked L-glycerol-3-phosphate dehydrogenase. The apparent kinetic parameters of the reaction catalyzed by the enzyme were affected by adriamycin. The effect of adriamycin was depe...

journal_title：Biochemical and biophysical research communications

authors： Beleznai Z,Jancsik V

更新日期：1989-02-28 00:00:00

abstract：:Alpha-/beta-dystroglycans (DG) located at the outmost layer of myelin sheath play a critical role in its formation and stability in the peripheral nerve system. The demyelination of nerve fibers is present in autoimmune neuritis, however, it is not known about the molecular mechanisms underlying this pathological proc...

journal_title：Biochemical and biophysical research communications

authors： Zhao XL,Li GZ,Sun B,Zhang ZL,Yin YH,Tian YS,Li H,Li HL,Wang de S,Zhong D

更新日期：2010-02-19 00:00:00

abstract：:Adiponectin is an adipocyte-derived hormone and known to form several species of multimer, however, the precise components of each multimer have not been fully determined. We purified each multimer adiponectin selectively from human plasma and characterized them by affinity columns using anti-adiponectin, gelatin, or ...

journal_title：Biochemical and biophysical research communications

authors： Hada Y,Yamauchi T,Waki H,Tsuchida A,Hara K,Yago H,Miyazaki O,Ebinuma H,Kadowaki T

更新日期：2007-05-04 00:00:00

abstract：:A polyclonal antibody against a MAP kinase (DdERK2) in Dictyostelium has been made and used to study DdERK2 activation and localization. The activation of DdERK2 by the chemoattractants cAMP and folate is rapid and transient. Its activity peaks between 15 and 60 seconds after cAMP stimulation and declines to basal lev...

journal_title：Biochemical and biophysical research communications

authors： Wang Y,Segall JE

更新日期：1998-03-06 00:00:00

abstract：:Endothelialization has been considered a promising method to improve the biocompatibility of vascular implanted biomaterials. However, little is known about the anti-coagulation, anti-inflammatory, anti-atherosclerosis and anti-shedding property of the attached endothelial cells (ECs) and the relationship with their b...

journal_title：Biochemical and biophysical research communications

authors： Li J,Zhang K,Wu J,Liao Y,Yang P,Huang N

更新日期：2015-01-09 00:00:00

abstract：:Recent data has suggested a role for nitric oxide (NO) both in the induction of immunity and as an effector of tissue injury in experimental models of inflammation. In this study, we have tested the efficacy of two inhibitors of NO synthase, NG-monomethyl-L-arginine (L-NMMA) and aminoguanidine (AG), to modify the auto...

journal_title：Biochemical and biophysical research communications

authors： Woolfson RG,Qasim FJ,Thiru S,Oliveira DB,Neild GH,Mathieson PW

更新日期：1995-12-14 00:00:00

abstract：:The results presented here show that a decrease in the concentration of total polyamines, due to a decrease in putrescine and spermine, occurs during oogenesis in Xenopus laevis. The microinjection of spermine or spermidine decreases the hormonal responsiveness (maturation) of the fully-grown oocytes. This effect is s...

journal_title：Biochemical and biophysical research communications

authors： Osborne HB,Mulner-Lorillon O,Marot J,Belle R

更新日期：1989-01-31 00:00:00

abstract：:Glutathione (GSH) is the most abundant non-protein thiol that buffers reactive oxygen species in the brain. GSH does not reduce nitroxides directly, but in the presence of ascorbates, addition of GSH increases ascorbate-induced reduction of nitroxides. In this study, we used electron paramagnetic resonance (EPR) imagi...

journal_title：Biochemical and biophysical research communications

authors： Emoto MC,Matsuoka Y,Yamada KI,Sato-Akaba H,Fujii HG

更新日期：2017-04-15 00:00:00

abstract：:The chloroethylnitrosoureas are useful antitumor agents which evidently exert a significant part of their cytotoxic action through the formation of a unique 1-(3-deoxycytidyl), 2-(1-deoxyguanosinyl)ethane cross-link in DNA. It has been suggested that this cross-link is formed from O6-(2-chloro-ethyl)guanine, an initia...

journal_title：Biochemical and biophysical research communications

authors： Parker S,Kirk MC,Ludlum DB

更新日期：1987-11-13 00:00:00

abstract：:Hartnup disorder is an autosomal recessive phenotype involving a transporter for monoamino-monocarboxylic acids. Genetic analysis of the mouse model mapped its locus to human chromosome 11q13 (8). We report here the results of linkage analysis in two Japanese first cousin-marriage families. In the first family, the pr...

journal_title：Biochemical and biophysical research communications

authors： Nozaki J,Dakeishi M,Ohura T,Inoue K,Manabe M,Wada Y,Koizumi A

更新日期：2001-06-08 00:00:00

abstract：:Tumor protein D52-like proteins (TPD52) are small coiled-coil motif bearing proteins that were first identified in breast cancer. TPD52 and related proteins have been implicated in cell proliferation, apoptosis, and vesicle trafficking. To date, three human TPD52 members had been identified, named hD52 (TPD52), hD53 (...

journal_title：Biochemical and biophysical research communications

authors： Cao Q,Chen J,Zhu L,Liu Y,Zhou Z,Sha J,Wang S,Li J

更新日期：2006-06-09 00:00:00

abstract：:The aim of this study was to investigate the response to and the physiological consequences of copper-mediated cross-linking of S100A2 and S100A4, two members of the S100 family of EF-hand calcium-binding proteins. As demonstrated by electrophoresis and mass spectrometry techniques S100A2 and S100A4 show formation of ...

journal_title：Biochemical and biophysical research communications

authors： Haase-Kohn C,Wolf S,Lenk J,Pietzsch J

更新日期：2011-09-30 00:00:00

abstract：:Inactivated vaccines are widely used for prevention of viral disease. Both humoral and cellular immune responses have been shown to play important roles in the control and clearance of virus infections. CpG motif containing oligodeoxynucleotides (ODN) have recently gained considerable interest and been used as vaccine...

journal_title：Biochemical and biophysical research communications

authors： Liang H,Xiao X,Zhang X,Hu Q,Yang Y,Cen S,Deng X,Sheng W

更新日期：2019-04-02 00:00:00

abstract：:Sphingolipids have been reported to regulate the growth and death of mammalian and yeast cells, but their precise mechanisms are unknown. In this paper, it was shown that the deletion of the oxysterol binding protein homologue 3 (OSH3) gene confers hyper resistance against ISP-1, an inhibitor of sphingolipid biosynthe...

journal_title：Biochemical and biophysical research communications

authors： Yano T,Inukai M,Isono F

更新日期：2004-02-27 00:00:00

abstract：:To elucidate whether PTH(7-84), a degradation product of PTH(1-84), which inhibits PTH(1-84)-induced bone resorption, also exerts an antagonistic effect on the kidney, we studied the effect of PTH(7-84) on PTH(1-34)-induced production of 1,25-(OH)2D3 in primary cultured murine renal tubules. Neonatal mouse renal tubul...

journal_title：Biochemical and biophysical research communications

authors： Nakajima K,Nohtomi K,Sato M,Takano K,Sato K

更新日期：2009-04-03 00:00:00

abstract：:Studies on the physiological role of motilin, and more recently, on the relationship between motilin and erythromycin A, have been hampered by the lack of antagonists. We now have discovered such a compound. ANQ-11125 displaces motilin bound to an homogenate of rabbit antral smooth muscle tissue. The dissociation cons...

journal_title：Biochemical and biophysical research communications

authors： Peeters TL,Depoortere I,Macielag MJ,Dharanipragada R,Marvin MS,Florance JR,Galdes A

更新日期：1994-01-28 00:00:00

abstract：:maf is a family of oncogenes, originally identified from the avian oncogenic retrovirus AS42, which encodes a nuclear bZip transcription factor protein. It has been reported that maf family genes have critical roles in embryological development and cellular differentiation. In this study, the distribution of maf-1 gen...

journal_title：Biochemical and biophysical research communications

authors： Imaki J,Onodera H,Tsuchiya K,Imaki T,Mochizuki T,Mishima T,Yamashita K,Yoshida K,Sakai M

更新日期：2000-06-16 00:00:00

abstract：:An Aspergillus niger (ficuum) genomic DNA lambda EMBL3 library was probed with a 354-bp DNA fragment obtained by polymerase chain reaction of A. niger DNA with oligonucleotides based on partial amino acid sequence of a pH 2.5 optimum acid phosphatase. A clone containing a 1605 bp segment (phyB) encoding the 479 amino ...

journal_title：Biochemical and biophysical research communications

authors： Ehrlich KC,Montalbano BG,Mullaney EJ,Dischinger HC Jr,Ullah AH

更新日期：1993-08-31 00:00:00

abstract：:The small heat shock protein hsp27 is associated with aggressive tumor behavior in certain subsets of breast cancer patients. Previously we demonstrated that hsp27 overexpression in breast cancer cells increased in vitro and in vivo invasiveness, suggesting that hsp27 influences the metastatic process. To investigate ...

journal_title：Biochemical and biophysical research communications

authors： Hansen RK,Parra I,Hilsenbeck SG,Himelstein B,Fuqua SA

更新日期：2001-03-23 00:00:00

abstract：:To examine the possible role of lipids as adhesion receptors for infection, Chlamydia trachomatis and Chlamydia pneumoniae were labeled with 125I and layered on thin-layer chromatograms (tlc) of separated lipids isolated from target cells, and bound bacteria were detected by autoradiography. Elementary bodies from bot...

journal_title：Biochemical and biophysical research communications

authors： Krivan HC,Nilsson B,Lingwood CA,Ryu H

更新日期：1991-03-29 00:00:00

abstract：BACKGROUND:Autophagy and apoptosis are two important regulators of cell survival, and are often observed simultaneously in response to noxious stimuli. Anoxia is a known stimulus for autophagy and apoptosis, and angiotensin (Ang) II is a major mediator of anoxic injury. However, specific responses to anoxia and Ang II ...

journal_title：Biochemical and biophysical research communications

authors： Wang X,Dai Y,Ding Z,Khaidakov M,Mercanti F,Mehta JL

更新日期：2013-11-01 00:00:00

abstract：:Strong temperature dependent low angle X-ray scattering by trifluoroethanol- and hexafluoro-2-propanol-water mixtures was observed in conditions commonly used in NMR work on peptides. At least two types of molecular effects may contribute to the observed scattering: formation of clathrate hydrate-like aggregates of al...

journal_title：Biochemical and biophysical research communications

authors： Kuprin S,Gräslund A,Ehrenberg A,Koch MH

更新日期：1995-12-26 00:00:00

abstract：:The heat shock response is known to inhibit NF-kappaB activation and NF-kappaB-dependent gene expression. Herein we determined if cells lacking heat shock factor-1 (HSF-1), the major transcription factor regulating heat shock protein gene expression, have an altered ability to modulate NF-kappaB activation. Embryonic ...

journal_title：Biochemical and biophysical research communications

authors： Malhotra V,Eaves-Pyles T,Odoms K,Quaid G,Shanley TP,Wong HR

更新日期：2002-03-01 00:00:00

abstract：:Sex determining region Y-box 2 (SOX2) is well known as one of the "stemness" factors and is often expressed in cancers including breast cancer. In this study, we developed a reporter system using fluorescent protein driven by the promoter for SOX2 gene to detect and isolate living SOX2-positive cells. Using this syste...

journal_title：Biochemical and biophysical research communications

authors： Liang S,Furuhashi M,Nakane R,Nakazawa S,Goudarzi H,Hamada J,Iizasa H

更新日期：2013-07-26 00:00:00

abstract：:The lipase from Malassezia globosa (SMG1) was identified to be strictly specific for mono- and diacylglycerol but not triacylglycerol. The crystal structures of SMG1 were solved in the closed conformation, but they failed to provide direct evidence of factors responsible for this unique selectivity. To address this pr...

journal_title：Biochemical and biophysical research communications

authors： Liu L,Gao C,Lan D,Yang B,Wang Y

更新日期：2012-07-27 00:00:00

abstract：:Cardiac fibrosis is an important cardiac remodeling event in the development of inflammation dilated cardiomyopathy （iDCM）. We have previously observed that degradation enhancer of androgen receptor (ASC-J9®) could improve cardiac inflammation and fibrosis. Using Primary CFs, we demonstrated that ASC-J9® attenuates th...

journal_title：Biochemical and biophysical research communications

authors： Wang Y,Ma W,Lu S,Yan L,Hu F,Wang Z,Cheng B

更新日期：2018-11-17 00:00:00

abstract：:Although the regeneration of the adult liver depends on hepatic progenitor cells (HPCs), many uncertainties regarding hepatic regeneration in the injured liver remain. Trefoil factor family 1 (TFF1), a secretory protein predominantly expressed in the gastrointestinal tract, is responsible for mucosal restitution. Here...

journal_title：Biochemical and biophysical research communications

authors： Hayashi Y,Yamaguchi J,Kokuryo T,Ebata T,Yokoyama Y,Nagino M

更新日期：2018-11-17 00:00:00

abstract：:Kruppel-like factor 2 (KLF2) is a crucial anti-angiogenic factor. However, its precise role in hepatic angiogenesis induced by liver sinusoidal endothelial cells (LSECs) remain unclear. This study was aimed to evaluate the effect of KLF2 on angiogenesis of LSECs and to explore the corresponding mechanism. Cultured hum...

journal_title：Biochemical and biophysical research communications

authors： Zeng XQ,Li N,Pan DY,Miao Q,Ma GF,Liu YM,Tseng YJ,Li F,Xu LL,Chen SY

更新日期：2015-09-04 00:00:00